Desenvolvimento da microscopia de força química usando modelagem molecular
Abstract
In this work was developed a prototype of a new nanobiosensor with molecular specificity through a study of theoretical models of Chemical Force Microscope. For the sensing were used molecular modeling techniques as well as experimental models of the functionalized Atomic Force Microscope tip with the Acetil co-A Carboxylase (ACC) attached. Specific and non-specific inhibitors were used to evaluate substrate-enzyme interactions. The nanobiosensor investigates specific enzymatic inhibition characteristics of the ACC enzyme through the herbicide Diclofop by reversing this process applying a force in a determined direction. The force is theoretically calculated by using molecular dynamic techniques associated to the adhesion force experimentally obtained. Theoretical and experimental questions involving nanobiosensors of AFM tips still obscure until now, such as, the number of functional enzymes attached on the AFM tip, the number of the active sites available to interact after immobilization process, the consequences of the enzyme immobilization as well as the substrate and theoretical adhesion between AFM tip and substrate were analyzed here.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Avaliação dos efeitos de polimorfismos e da origem parental do alelo na expressão de genes candidatos à característica maciez da carne em bovinos da raça Nelore
Souza, Marcela Maria de (Universidade Federal de São Carlos, UFSCar, Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv, , 29/02/2012)Tenderness is the main trait appreciated by consumers of bovine meat, however Nellore animals that comprise the largest part of Brazilian cattle, have lower tenderness when compared with European animals. In this way, it ... -
Associação entre polimorfismos no gene da glicoproteína-P (PgP) e resistência múltipla a anti-helmínticos em Haemonchus contortus e identificação de fatores de risco relacionados
Mello, Suelen Scarpa de (Universidade Federal de São Carlos, UFSCar, Programa de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEv, , 28/02/2014)Among the sheep parasites, Haemonchus contortus is the most prevalent and pathogenic nematode in tropical areas, causing huge economic losses. Alterations in the gene encoding the membrane P-glycoprotein (PgP) have been ... -
Estudo do potencial eletrostático e da termodinâmica de agregação de surfactantes por simulações de dinâmica molecular
Bernardino, Kalil (Universidade Federal de São Carlos, UFSCar, Programa de Pós-Graduação em Química - PPGQ, Câmpus São Carlos, 13/03/2018)Due to the amphiphilic character, the surfactants tend to be adsorbed at the water surface and, in concentrations larger than the critical micellar concentration, they self-assembling in solution with the purpose to reduce ...