Método fundamentado em processamento digital de imagens para contagem automática de unidades formadoras de colônias
Resumo
This work presents a computer aided system for automated counting of colony formation units
in Petri dishes. The method implemented uses digital image processing, and among them Hough
transform for circular objects and Match for correlation with objects having different forms. The
method was developed based on the visual Borland Builderr C++ environment, and for the image
acquisition a luminance equalization system was used to allow stabilized and continuous 1,200 lux.
For the validation of the method, samples containing Acidithiobacillus ferrooxidans, Escherichia
Coli, and Staphylococcus aureus were analyzed. Results have shown the potentiality of the methodology,
which is applied for laboratory applications that involved the quantification of bacterial
colonies in a solid culture environment.