Efeitos da L-histamina e de antagonistas histaminérgicos sobre a recuperação funcional após hemilabirintectomia em Carassius auratus.
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Data
2006-06-02Autor
Ferrara, Aline Cristina Piratello
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The aim of this study was to verify the effects of histamine on the process of functional
recovery after hemilabyrinthectomy in goldfish, Carassius auratus. To this end, the fish were
submitted to unilateral vestibular lesion and treated with a histamine precursor (L-histidine)
and antagonists of the receptors H1, H2 and H3 (respectively chlorpheniramine, zolantidine
and thioperamide). The success of the surgery was evidenced by the ataxia they suffered
during approximately thirty minutes of observation and the presence of these signs was used
as criterion for the inclusion of the animal in the experiment. The subjects were treated during
fifteen consecutive days with L-histidine (150 mg/kg), chlorpheniramine (24 mg/kg),
zolantidine (18 mg/kg) and thioperamide (15 mg/kg). All substances were administrated in a
volume of 1.5 ml/ kg of body weight and sterile saline (1.5 ml/kg) was used with the
experimental control group. Another group, the sham lesion group, was submitted to the same
surgical procedure, but in this case the vestibular lesion was not carried out. The subjects were
submitted to seven tests, the first test being carried out on the first day after the lesion, and
then five more at three day intervals. The seventh test was carried out three days after the
conclusion of treatment. In each test, the subject was placed inside a translucent tube inside a
black aquarium which was illuminated on the ipsilateral side for five minutes. The fish
behavior was recorded with a video camera located in front of a small opening in the front
wall of the aquarium. Body tilt was measured by comparing a line traced between the
subject s eyes with a horizontal line traced on the television screen. The mean of the tilt in
degrees obtained every thirty seconds was considered. The data were subjected to variance
analysis (Two-way ANOVA), followed by the Student Newman Keuls multiple comparisons
test.