O treinamento resistido previne a disfunção mitocondrial no córtex pré-frontal e no músculo vasto lateral de ratas ovariectomizadas
Resumen
Introduction: As we age, human body goes through several important physiological
changes, especially in women after menopause, during which estrogen levels are declining,
causing changes in mitochondrial function both in skeletal muscle and brain. Menopause is
mimicked experimentally by a technique called ovariectomy, that causes some alterations in
rats, such as the loss of muscle mass (sarcopenia); however studies have demonstrated that
loss of age-related muscle is a different process from strength loss. Whereas the loss of
muscle strength is dissociated from this condition, it was renamed as dinapenia. Resistance
Training (RT) has beneficial effects on postmenopausal women, but is not well established
in the literature the possible effects of RT on mitochondrial function of the cerebral motor
cortex and skeletal muscle of ovariectomized rats. Objective: This study aimed to investigate
the effects of ovariectomy and resistance training on mitochondrial function (oxygen
consumption) in tissue biopsy of the prefrontal cortex and vastus lateralis muscle (VL) in
rats. Materials and Methods: were used 40 adult female rats divided into 4 experimental
groups (Sham-SED, Sham- TR, OVX-SED and OVX-TR). The ovariectomy was
performed at 13 weeks. The Resistance Training (RT) proposed was the climb stairs
model, and held for 13 weeks. The masses of the prefrontal cortex, cerebellum, Vastus
Lateralis (VL) and Retus Femoralis (RF) were analyzed after sacrifice, which took place 48
hours after the last training session. Oxygen consumption and mitochondrial respiratory
parameters were performed on tissue biopsy of the prefrontal cortex and the vastus lateralis
(VL) by technique of High Resolution Respirometry (Oroboros®). Results: it was found
decreased muscle mass (VL and RF), as well as the cerebellum in OVX-SED group
compared to other groups (p <0.05). TR prevented the loss of muscle and cerebelar mass.
Mitochondrial oxygen consumption was decreased in OVX-SED group compared to the
other experimental groups (p <0.05), effect once again prevented by TR. Conclusion:
ovariectomy produced mitochondrial dysfunction in the prefrontal cortex and VL,
deleterious effect prevented by RT.