Efeitos da fototerapia em marcadores inflamatórios, na função mitocondrial e no estresse oxidativo em roedores com diabetes induzida
Visualizar/ Abrir
Data
2016-05-09Autor
Rocha, José Carlos Tatmatsu
Metadata
Mostrar registro completoResumo
Tissue repair in diabetic patients is hampered by several factors, including secretion of
insulin deficiency that leads to hyperglycemia and production of free radicals.
Mitochondrial dysfunction has been identified as one of the probable factors of DM. It
is known that phototherapy has proliferative effects in tissues with some pathologies.
This study aimed to evaluate the effects of phototherapy using laser and low power
LED on tissue regeneration, oxidative stress and mitochondrial markers in diabetic
animals. Methodology: 20 Swiss mice (30g, 6 weeks) for the study relating to oxidative
stress and 20 Wistar rats (250 g, 8 weeks) were subjected to induction of DM
(streptozotocin injection 70 mg / kg) and after 120 days follow up, suffered skin lesions
and were treated with Low Level Laser Therapy and LED for 5 consecutive days and on
day 6 the animals were killed and the skin removed. Another group of animals (20 male
Wistar rats weighing 250 g, 8 weeks) was subjected to laser irradiation and in the LED
region of the pancreas for 5 consecutive days and on day 6 animals were killed and the
pancreas, liver, and gastrocnemius muscle were removed. Animals were divided into
four groups: Sham (healthy control), NID (non-irradiated diabetic), DLED (diabetic
irradiated by LED) and DLASER (diabetic irradiated by LED). Levels of catalase,
thiobarbituric acid (TBARS), nitrite, collagen content, vascular endothelial growth
factor (VEGF), cyclooxygenase-2 (COX2) Mitofusin 2 (MFN2), Mitochondrial fission
1 protein (FIS1) liver and muscle glycogen and functional insulin and glucose tests were
measured. Results: It was observed in the skin of these animals, increase in collagen
content and angiogenesis in irradiated diabetic animals, TBARS levels and lower nitrite
in irradiated diabetic. Furthermore, COX-2 expression was higher among NID
compared to SHAM and DLED groups. DLED showed the highest scores against
MFN2 and FIS1 expression was higher in DLASER group. Laser and LED Therapy
altered the expression of VEGF and COX2 and stimulated the proliferation of mature
collagen fibers in diabetic animal wounds. About irradiation over pancreas results
showed that the NID group, hepatic and muscle glycogen content were statistically
lower than DLASER and DLED groups. Adding to these data, the density of the
pancreatic duct and pancreatic islets were significantly different when compared to
NID. These results suggest that both the low-level laser LED obtained as potential
therapeutic effects on the skin and pancreas under conditions hyperglycemic and that
both therapies acted modulating histological, angiogenic and mitochondrial parameters
on pancreas and skin of diabetic animals.