Complexos metálicos luminescentes com ácidos fenólicos coordenados: alvos seletivos a mitocôndrias e mapeamento da atividade antioxidante in vitro e in vivo por técnicas fluorescentes

Abstract

In this work, the syntheses and characterizations of the complexes are presented, cis-[Mg(phen)(iso)(H2O)3](CH3COO) and [Mg(RPA)(iso)(H2O)(OH)], where phn =phenanthroline, iso= isovanillic , and N-(9-(2-(((4-((1,10-phenanthrolin-5-yl)carbamoyl)benzyl)oxy)carbonyl)phenyl)-6-ethylamino) -3H-xanthen-3-ylidene)-N-ethylethanaminium bromide (RPA) by elemental analysis of C, H, N and thermogravimetric analysis and by NMR of 1H and 13C, 2D COSY and DOSY. Compared to isovanillic acid, the complexes obtained are stable in the solid state, in aqueous solution and are not photodegradable. The insecticidal activity of cis-[Mg(phen)(iso)(H2O)3](CH3COO) in the form of the baits (2.0mgg-1) investigated against leaf-cutting ants of the genus Atta sexdens rubropilosa presented a mode of action slow with 100% mortality on the sixth day, S50 = 2b, and complete inhibition of the development of the symbiotic fungus leucoagaricus gongylophorus. This complex did not show any toxicity to aquatic environment when exposed to paulistinha fish (Zebrafish) for 96 hours at a concentration of 2.0 mgL-1. It also showed no toxicity in the terrestrial environment. In this chronic study during 21day, male Wistar rats were treated with cis-[Mg(phen)(iso)(H2O)3](CH3COO) complex saline solution via intraperitoneal (3.0 mg/kg). Behavioral studies (open field, forced swimming and high cross labyrinth) have suggested that this complex possesses characteristics of anxiolytic drugs. Complex I has high inhibition capacity to oxygen reactive species (1O2, O2•‾), with IC50 below 100M. It has presented excellent inhibition of lipid peroxidation (ROO•, LO•) investigated in pork samples with IC50 at the scale 500 mmolL-1. complex, presented in this work, opens a new horizon of applications in agricultural, medic, and food areas.