Detecção e identificação de micobactérias em corpos de água destinados à captação para abastecimento urbano da cidade de São Carlos-SP
Abstract
Fifty-nine water samples from Feijão River, Monjolinho River and
the Water Treatment Station (SAAE) were analyzed, and 51 were positive for
mycobacteria. Concerning the two procedures of decontamination, we obtained
83.0% of positive samples isolated with sulfuric acid and 39.0% with
cetylpyridinium chloride. We recovered 425 strains of mycobacteria, and 402
were characterized concerning growth rate and pigment production. In drinking
water samples there were predominance of mycobacteria with slow growth,
wherein 66.7% were scotochromogenics species and 47.5% were identified as
M. lentiflavum. The identification was done through biochemical tests and
phenotypics features (classical methodology), micolic acid analyses by thinlayer
chromatography (TLC) and PCR-Restriction Analysis (PRA). Through the
classical methodology associated to TLC 33.3% of the mycobacteria were
identified and 18 species were defined. From the 402 mycobacteria 93 strains
were selected and submitted to PRA, being 35 identified by the classical and
molecular methodology, 51 only identified by PRA and 40% remained obscure
concerned identification. The PRA pattern 440 in BstEII and 130/110 in HaeIII
was defined as M. new. This pattern was found for 24 strains. For an accurate
identification, the association of different methodologies is necessary.