Metabólitos secundários de Streptomyces clavuligerus ATCC 27064 e fungos endofíticos filamentosos

Abstract

This work describes the investigation of part of the secondary metabolism of actinomicete Streptomyces clavuligerus and endophytic filamentous fungus Penicillium sp isolated from Murraya paniculata; Penicillium brasilianum and Aspergillus aculeatus isolated from Melia azedarach and Penicillium griseoroseum isolated from the grains of Coffea arabica. Preliminary tests were realized to define the best composition of medium culture and the best condition of cultivation Streptomyces clavuligerus. On the other hand, the endophytic fungus were cultivated in rice and Czapec´k (medium liquid). The anthranilic acid and the 7-hydroxy-2- methyl-chromone were identified from the extracts of S. clavuligerus by spectroscopic methods 1D e 2D NMR and mass spectrometry. Methodologies of analysis for detection of β-lactam antibiotics were developed by liquid chromatography combined with mass spectrometry. The identification was based on studies of standard compounds and comparison with literature data. These methodologies were efficient in the analysis of this type of substances and they allowed the detection and identification of β-lactam antibiotics such as the penicillin N, the cephamycin C, deacetoxy-cephalosporin C and the clavulanic acid in small amounts in S. clavuligerus the extracts. In the endophytic fungus extracts were possible to identify the 6-aminopenicillanic acid, precursor of the penicillin G and metabolites no β-lactams as nucleotides thymine and adenine.