Avaliação comportamental e caracterização eletrofisiológica de juvenis de Tambaqui (Colossoma macropomum) submetidos à anestesia com propofol e óleo essencial de Nepeta cataria

Abstract

The new drugs are new like unproven fish anesthetics, although they have behaviors of induction and anesthetic recovery, only the monitoring of the electrophysiological results and the verification of the cardiorespiratory and neuronal activity of power over the confirmation and the anesthetic potential of the agent tested. The study was carried out with tambaqui juveniles, Colossoma macropomum through the monitoring of behavioral and electrophysiological occurrences, where its efficacy under the effect of anesthetic agents, cardiac support, electrocardiogram, heartbeat (FBO) and intensity of opercular beats (IBO) and electromyography, in addition to its antioxidant capacity. Incredible catary (ENP) and the essential of propofol (PRP) and the oil in which were tested from tests that were tested six PRP and OEN for agreement with preliminaries for the definition of tracks-test, a was able to provoke the sedimentation capacity in approximately 3 min and recovery in approximately 5 min for these tests were used in animals (n = 10) and the test times for each stage of sedation and anesthesia recovery, where a concentration ideal of 175 μL L - 1 for EON and 4 μL L - 1 for PRP. For electromyographic evaluation, electrocardiographic data, intensity of operative beats (OBI) and rate (OBR), fish (5.6 ± 1.8g, 8.0 ± 0.3cm, total length) were attributed to the following groups: a) control (baseline b) fish exposed to ethanol (control vehicle), c) fish submitted to induction with 175 μL L-1 EON and subsequent recovery and d) fish submitted to induction with PRP at 4 μL L-1 and subsequent recovery. Eight fish (n = 8) per group were used. Both products induced complete immobilization of the body and suppressed the contraction power of the dorsal muscle (99.9%), determining a state similar to general anesthesia. During exposure to any of the anesthetics, cardiac function was affected transiently, with decreased heart rate (42 - 43%) and mild arrhythmia in fish in PRP recovery. In addition, ventilatory capacity was reduced with this drug (85.3% and 59.6% reduction in OBI and OBR, respectively), but without mortality. On the other hand, EON did not alter ECG tracings, permitting maintenance of normal ventilation intensity during exposure, although a slight increase in OBR during deep anesthesia occurred. This study confirms the anesthesia with propofol in fish and presents the essential oil of Nepeta cataria as a new and safe natural anesthetic for juveniles of Colossoma macropomum and potentially for other species of teleosts.