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Estudo da expressão de lipase BTL2 de Bacillus thermocatenulatus em E. coli recombinante
(Universidade Federal de São Carlos, 2009-04-03)
Using recombinant DNA techniques, the lipase BTL2 gene of Bacillus thermocatenulatus was cloned in E. coli BL321 under control of the strong temperature-inducible λPL promoter. It
was investigated, initially, in this ...
Separação de compostos com atividade antibacteriana obtidos a partir de cultivos de Streptomyces clavuligerus em meio complexo
(Universidade Federal de São Carlos, 2009-09-01)
The cephamycin C is a natural cephalosporin produced by Streptomyces clavuligerus, of great importance in the pharmaceutical industry; it is a β-lactam antibiotic that acts as an inhibitor of the cellular wall synthesis. ...
Desenvolvimento e otimização do processo fermentativo para a produção de vinho branco a partir da uva Itália.
(Universidade Federal de São Carlos, 2005-08-19)
With the reduction of vineyards in São Roque, the wine industry of this region has been seeking for alternatives to produce common wine with low cost. In the present work, wine production from Italia grape obtained at the ...
Avaliação de variáveis operacionais e aplicação de enfoque híbrido na modelagem do processo de produção de penicilina G acilase por Bacillus megaterium.
(Universidade Federal de São Carlos, 2005-09-08)
In the present work it was studied the influence of operational conditions particularly dissolved oxygen concentration in the penicillin G acylase (PGA) production. The
experiments were carried out in aerated and agitated ...
Avaliação de transferência de calor e massa em biorreator Airlift de bancada para a produção de ácido clavulânico.
(Universidade Federal de São Carlos, 2005-03-28)
The importance of the airlift bioreactor has grown in the biotechnology
field by aspects as high oxygen transfer, with power input low.
In this work were study the conditions heat and mass transfer, as well
as the condenser ...
Avaliação diferencial de fatores fisiológicos e cinéticos em cultivos de linhagens de células selvagem e recombinante de Drosophila melanogaster S2
(Universidade Federal de São Carlos, 2005-09-19)
In the last 10 years the insect cell Drosophila melanogaster Schneider S2 has been increasingly used for studies of proteins recombinants. However, there is a lack of studies that involved parameters related to the use of ...
Contribuição ao desenvolvimento de metodologia para detecção de desintegrina recombinante produzida em cultivos de células CHO-K1.
(Universidade Federal de São Carlos, 2004-06-25)
Disintegrins are proteins present in the poison of serpents that have been calling the pharmaceutical industry attention due to their capacity to prevent the progression of cancerous cells. A receiving key-protein called ...
Separação e purificação do ácido clavulânico através de extração líquido-líquido seguida de adsorção em coluna de leito fixo.
(Universidade Federal de São Carlos, 2005-10-19)
A study was made of the extraction of clavulanic acid (CA) from a fermented medium and
its concentration and purification, based on a comparison of two liquid-liquid extraction
methods, followed by adsorption in a fixed ...
Estudo de condições operacionais na produção de penicilina G acilase por diferentes microrganismos
(Universidade Federal de São Carlos, 2006-12-20)
Penicillin G acylase (PGA) is an enzyme of major impact in human health for
catalyzing the deacilation of the penicillin G, to yield 6-aminopenicillanic acid (6-
APA), key intermediate in the semi-synthetic antibiotics ...
Avaliação da velocidade de cisalhamento média em biorreator convencional tipo tanque agitado e aerado
(Universidade Federal de São Carlos, 2007-03-12)
In biochemical processes, shear rate ( γ&) in the system is an important variable on design
and operation of bioreactors. Shear can result in damage to the cells and the microorganisms,
taking to the viability loss ...