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Imobilização da enzima β-galactosidase de Kluyveromyces fragilis em agarose e quitosana utilizando diferentes protocolos de ativação
(Universidade Federal de São Carlos, 2009-02-27)
The objective of this work was stabilize and immobilize β-galactosidase from activated agarose and chitosan supports. Initially, it was evaluated the buffer type, ionic strength and bivalent ions Mn2+ and Mg2+ on the ...
Pré-tratamento alcalino (NaOH) do bagaço de cana-de açúcar para a produção de etanol e obtenção de xilooligômeros
(Universidade Federal de São Carlos, 2011-03-28)
The aim of this study was to optimize the pretreatment of sugarcane bagasse raw and exploded with diluted sulfuric acid, water and sodium hydroxide in order to obtain Xylooligosaccharides in hemicellulose and production ...
Influência da linhagem da levedura e das condições de cultivo no processo de isomerização e fermentação simultâneas da xilose
(Universidade Federal de São Carlos, 2013-03-21)
The conversion of the hemicellulosic fraction in ethanol is a factor that impacts on the economic viability of the second generation ethanol production process from sugar cane bagasse. Hemicellulose from bagasse is a ...
Estudo de condições operacionais na produção de penicilina G acilase por diferentes microrganismos
(Universidade Federal de São Carlos, 2006-12-20)
Penicillin G acylase (PGA) is an enzyme of major impact in human health for
catalyzing the deacilation of the penicillin G, to yield 6-aminopenicillanic acid (6-
APA), key intermediate in the semi-synthetic antibiotics ...
Secagem convectiva de folhas de hortelã: análise baseada no ajuste de correlações empíricas, superfícies de respostas e redes neurais
(Universidade Federal de São Carlos, 2013-03-15)
Mentha x villosa H. is a species of mint popularly known as regular mint. Besides being widely used as a spice, their leaves yield an essential oil with many healthy compounds that are commonly used in formulation of ...
Quantificação de apoptose e necrose mediante corantes fluorescentes e análise de imagens, no cultivo de células de inseto: o caso da Drosophila melanogaster S2
(Universidade Federal de São Carlos, 2007-03-28)
In animal cell cultures there are two kinds of cell death: apoptosis
(programmed cell death) and necrosis (cell death due to external sources). The inovative
method of cell death identification that have attracted interest ...
Aplicação da lógica fuzzi na produção de penicilina G Acilase em cultivos de Bacillus megaterium
(Universidade Federal de São Carlos, 2003-07-28)
Penicillin G acylase (PGA) is an important enzyme for the pharmaceutical industry. This enzyme has industrial use in the hydrolysis of penicillin G to obtain 6-aminocephalosporanic acid (6-APA), essential intermediate for ...
Aspectos morfológicos, reológicos e fisiológicos dos cultivos de Escherichia coli recombinante
(Universidade Federal de São Carlos, 2015-04-30)
The use of Escherichia coli cultures to obtain heterologous proteins poses as a successful application of biotechnology for the production of drugs and enzymes. However, the stress caused by the synthesis of the recombinant ...
Estratégias para a síntese de agregados reticulados porosos de lipase de pâncreas de porco com propriedades magnéticas
(Universidade Federal de São Carlos, 2019-02-28)
Cross-linked enzyme aggregates (CLEAs) is an immobilization technique that does not require solid support, allows the use of semi-purified enzymes and the biocatalyst show higher volumetric activity. However, they present ...
Solubilização biológica de rocha potássica para aplicação como biofertilizante
(Universidade Federal de São Carlos, 2020-02-18)
Potassium (K) plays an important role in several biochemical and physiological processes in plants, but the low concentrations of soluble K in the soil make it necessary to use chemical fertilizers in order to supply the ...