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Identificação e clonagem de genes espécie-específicos de Leishmania infantum para expressão piloto de proteínas recombinantes com potencial para o diagnóstico de Leishmaniose Visceral
(Universidade Federal de São Carlos, 2023-02-16)
Leishmaniasis is a public health problem observed in several countries, being considered by the World Health Organization (WHO) one of the 10 neglected tropical diseases. It is caused by Leishmania parasites that affect ...
Expressão recombinante e ensaios de atividade de duas thaumatin-like proteins do Maqui (Aristotelia chilensis)
(Universidade Federal de São Carlos, 2022-10-24)
Thaumatin-like Proteins (TLP) are members of the Pathogenesis-Related Proteins family 5 (PR-5), and are present in fungi, plants and invertebrates. In plants, these proteins are involved both in the host’s defense, often ...
Efeito da microvibração sobre a produção in vitro de embriões bovinos
(Universidade Federal de São Carlos, 2022-11-07)
Much effort has been employed to improve the quality of embryos obtained by in vitro production (IVP) given the relevance of this technology to current livestock systems. In this context, dynamic IVP systems have been shown ...
Cistatinas de maqui: produção recombinante e inibição de cisteíno peptidases
(Universidade Federal de São Carlos, 2022-02-15)
Cystatins are tight binding competitive inhibitors of cysteine peptidases. In plants, they act regulating different physiological mechanisms, as well as defending from biotic and abiotic stresses, making them of potential ...
Seleção e caracterização molecular e fermentativa de leveduras personalizadas para a produção de vinhos brasileiros
(Universidade Federal de São Carlos, 2022-03-04)
Brazilian wine production has been standing out with high quality awarded wines around the world. However, major changes are still needed so that our country can compete with other wineries in the world. Among them, we can ...
Estudo do efeito do silenciamento do gene ITGB3 em células tumorais de mama
(Universidade Federal de São Carlos, 2022-09-01)
Breast cancer is the major disease that affects women, especially the triple negative phenotype, which has the worst prognosis and metastatic potential. Included, metastasis is still the main cause of death in cancer ...
Análise da expressão gênica e conteúdo proteico de ABCB10, APEX1 e HMGB1 em pacientes com beta talassemia maior e intermediaria
(Universidade Federal de São Carlos, 2017-09-04)
Beta thalassemias (BT) are a group of hereditary diseases characterized by the partial or total suppression of beta globin chains synthesis. Clinically, it manifests itself in three distinct forms: minor (BTm), intermedia ...
Produção recombinante, caracterização enzimática e estudos sobre a ocorrência de pectinases no "bicudo da cana-de-açúcar" (Sphenophorus levis, Curculionidae)
(Universidade Federal de São Carlos, 2012-04-04)
Plant cell wall confers to the cell plant, structural support as well as protection against pathogens and phytophagous. Among the cell wall polysaccharides includes pectic substances, which are composed of partially ...
Isolamento e caracterização de cepas termotolerantes de Saccharomyces cerevisiae e análise da expressão de genes possivelmente envolvidos com a termotolerância
(Universidade Federal de São Carlos, 2018-03-28)
Brazil is the world’s second largest producer of ethanol, which is produced, in our country, from the fermentation of sugarcane sucrose by Saccharomyces cerevisiae yeast. Despite having a high capacity for ethanol production, ...
Produção heteróloga de inibidores de pectina metil esterase (PMEI), para ensaios de inibição com PME e Invertase, enzimas intestinais do Sphenophorus levis
(Universidade Federal de São Carlos, 2018-02-20)
The pectin is a fundamental part of the cell wall from plants and it is composed mainly by 4 polysaccharides. The polysaccharide most abundant is the homogalacturonan (HG) representing up to 65% from pectin, and it is a ...