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Pré-condicionamento em sementes de Cassia excelsa Schrad
(Universidade Federal de São Carlos, 2002-06-05) Jeller, Helma; Perez, Sonia Cristina Juliano Gualtieri de Andrade; https://lattes.cnpq.br/9973185407856723
Cassia excelsa is a woody species commun in cerrado and srubland of brazilian northeast, known by cassia-do-nordeste . It could be used in landscape place and due to small size is used in street arborization, and could be recommended to recomposition of degradated areas. The aim of this work was to extend the knowlege about the ecophysiological behaviour, with the the study of priming in seed germination. For this purpuse, it was evaluated the effects of water and PEG solutions (-0,2; -0,4 and -0,6 MPa) in uptake process at 20ºC. The effects of the drying and storage after priming; the answer under water, thermic and salt stress after priming was also verified. Besides it, the priming effects in seed viability and vigor under optimal conditions (27ºC) were observed by eletrical conductivity, accelerated aging and submersion tests. In all the tests was used 4 replicates of 25 seeds, with daily evaluation, and considered germinated when radicle was > 2 mm long. According to the results of uptake curve of Cassia excelsa seeds was observed that with the decreased in the osmotic potential increase the time necessary to priming. At optimal temperature (27ºC) the answer of primed seeds was efficient if water or PEG solution at -0,2 MPa was used, and this answer is maintened after 15 days of storage at 5ºC, but the drying of the seeds overcome the positive effects of the pré-treatment. The priming in water or in PEG solutions increased germination percentage under water, salt and thermal stress, however, with restriction to germination rate. The electrical conductivity of the primed seeds in water or in PEG was reduced, constrated to the non-primed seeds, and increased in the dried seeds after the priming. The priming in water or PEG was efficiente to overcome the deteriorative process occurring in aging, in dehidrated and in moist seeds, after priming. The seed vigor of primed seeds was no affected by submersion in distilled water during diferents time periods.
Caracterização estrutural e funcional de hemoglobinas de skua, Catharacta maccormicki
(Universidade Federal de São Carlos, 2002-06-05) Landini, Gustavo Fraga; Schwantes, Arno Rudi; https://lattes.cnpq.br/1515297609478949
Hemoglobin samples of Catharacta maccormiki (Skua), an Antarctic bird, collected in winter and in summer were studied. Starch gel eletrophoretic pattern hemoglobin oxygen affinities on blood and stripped hemoglobin; influence of organic phosphates that may change affinity and level of those intra-erythrocytic phosphates were analyzed. No differences were found in Hb-O2 affinities for samples collected in winter and in summer, which leads to believe that migration (Winter) is not a factor for the oxygen affinities in blood. Bohr effect for both situations was practically the same (Φ= -2,1), indicating normal Bohr effect. The 5 samples show a electrophoretic pattern of two components, so, no evidence of intra-specific variation was found. For the stripped hemoglobin the P50 values were inferior to the ones obtained for whole blood, showing a higher affinity for the hemoglobin to oxygen in the absence of possible modulators. The Bohr effect in the alkaline side (pH 7 8) was normal (Φ = -1,9), and in the acid side it was reverse, characteristic of birds hemoglobin. The influence of organic phosphates that could affect oxygen affinity was also tested. 2,3-diphosphoglycerate (2,3DPG) and inositol hexaphosphate (IHP) were added to the stripped hemoglobin in order to test them as modulators. Negative hemoglobin oxygen affinity modulation was observed after addition of both phosphates. IHP is more effective than 2,3DPG. For animal collected during winter only the GTP was present in its erythrocytes, while the summer ones ATP was found instead of GTP. No differences was found in the concentration of inositol penta and hexaphosphsphate in samples collected in the two seasons. No 2,3DPG were found in any sample.