Navegando por Data de Publicação, começando com "2003-01-24"

Filtrar resultados por ano ou mês

Agora exibindo 1 - 2 de 2

Estudo de polimorfismos bioquímicos e grupos sangüineos em cavalos das raças mangalarga e mangalarga marchador
(Universidade Federal de São Carlos, 2003-01-24) Lippi, Andréia Samaha; Mortari, Norma; https://lattes.cnpq.br/3043874405923556
Allelic frequencies at 12 loci (five blood groups: C, D, K, P, and U; and seven protein polymorphisms: Al, A1B, Es, Gc, Hb, PGD, and Tf), are given for two Brazilian horse breeds: Mangalarga Marchador and Mangalarga. The high genetic identity value found (96.0%) is consistent with their common origin, although, at some point of the development of Mangalarga Marchador, Mangalarga separated from the original stock. The expected average heterozygosity was higher in Mangalarga Marchador. The populations presented genetic differentiation, as shown by the statistically significant value of FST. The nonsignificant FIS values showed that there was no appreciable consanguineous mating in any of the two populations. Exclusion probability calculated for the 12 loci was 87.0% and 86.5% for Mangalarga Marchador and Mangalarga, respectively. No genetic equilibrium was observed in the A1B, Tf, and Es loci of Mangalarga Marchador. The frequencies of blood factors A, Q, and T were calculated.
Estudos das proteínas ligantes à DCRA e DSCR1 (envolvidas com a síndrome de Down) utilizando a metodologia do duplo-híbrido
(Universidade Federal de São Carlos, 2003-01-24) Silveira, Henrique César Santejo; Silva, Flávio Henrique da; https://lattes.cnpq.br/1757309852446263
The genes DCRA and DSCR1 are located in the Down Syndrome Critical Region , a specific portion of human chromosome 21 responsible for the main traits of the disease. In the present work we have used the two-hybrid system as an approach to find proteins that interact with DCRA and DSCR1. This method is based on the properties of the yeast GAL4 protein, which consists of separable domains responsible for DNA-binding and transcriptional activation. The open reading frames of DCRA and DSCR1 were cloned in frame with the GAL4 DNA-binding domain. These constructions were used to analyse protein interactions using a human foetal brain cDNA library, cloned in frame with the GAL4 transcriptional activation domain. No DCRA protein partners were found in any of the screenings performed; nevertheless, the analysis allowed the detection of several false positive clones. In the analysis of DSCR1 we identified two proteins, UXT (ubiquitously expressed transcript) and APLP1 (amyloid precursor-like protein 1). These results may help elucidate a new function for DSCR1 in the nucleus, probably related gene expresion.