Browsing by Issue Date, starting with "2005-05-24"

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Análise comparativa dos mecanismos de governança das redes agroindustriais avícolas da macrorregião de Ribeirão Preto SP e de Santa Catarina.
(Universidade Federal de São Carlos, 2005-05-24)
Pinotti, Raquel Nakazato
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Paulillo, Luiz Fernando de Oriani e
;
https://lattes.cnpq.br/9919942357089937
This text has as objective main to carry through a comparative analysis it enters the power network of being poltry of the macroregion of Ribeirão Preto of the state of São Paulo and of the state of Santa Catarina, taking for base the individual and collective action of the actors and its main dynamic resources of power (constitutional, technological, financial, legal politicians and organizacionais ). From the studies of the characteristics of the actors of each network (number of participants; function, types of relation, structure and distribution of being able), were possible to verify that the catarinense poltry network presents more dynamic resources of power, greater coordination and harmony between its actors. The catarinense network has presence of formal relations of production, significant presence of dynamic resources of power and the legitimacy of the great industrial firms in the coordination of the system of integration and the network, while that the São Paulo network presents sufficiently distinct configuration: more types of actors (the independent agricultural producers, the intermediate and the corrector of chicken), low representation of its actors, informal relations of production and minor presence of dynamic resources power. In this context, it was possible to conclude that the networks possess trajectories of dependence (path dependence) well distinct, that lead to the structure of the distribution of dynamic resources of power presented.
C-CORE : uma Ferramenta de programação para construção e reuso de componentes
(Universidade Federal de São Carlos, 2005-05-24)
Souza Neto, Raphael Marcilio de
;
Prado, Antonio Francisco do
;
https://lattes.cnpq.br/2668568143800755
;
https://lattes.cnpq.br/5956634284343253
The Software Development contains several activities that are not always supported by tools that aid the Software Engineer and automate great part of his activities. Different researches have been accomplished and many resources have been spent aiming to construct tools that support the Software Development Process. Among these tools, stand out those that support project and implementation activities, and that are integrated with tools that support other activities of the software construction process. With those tools, it can be obtained a larger consistence of the results that are produced along the whole software development cycle. Another important point of the development process is related to obtain a software with better quality and less cost. One of the areas that is highlighted in the software production with quality and less cost, is those that researches the software components reuse. Thus, the Software Component Oriented Programming Tools integrated with modeling tools that aim mainly to improve the software productivity based on reuse is standing out. Motivated by these ideas, this work presents a tool denominated C-CORE, that supports the project and implementation of components and their applications integrated with other modeling tool, denominated MVCASE.
Estudo do desempenho de um precipitador eletrostático operando na captura de partículas submicrométricas e nanométricas
(Universidade Federal de São Carlos, 2005-05-24)
Falaguasta, Maria Carolina Ribeiro
;
Coury, José Renato
The removal of submicron particles from gaseous streams is becoming an increasingly important operation, both in industrial (nanotechnology) as well as in occupational (inhalation of particula te matter) applications. Among the equipments commonly employed for the removal of particula tes of the gaseous stream, the electrostatic precipitator, ESP, is the one capable of removing particles of such size with high efficiency. Furthermore, it has other advantages as the low pressure drop and the flexibility of working at higher temperatures. Therefore, the objective of this research was to evaluate the influence of operational and design parameters in the efficiency of particle removal of a plate-wire ESP operating in the removal of submicron particles. The precipitator was built in acrylic, with copper plates as collecting electrodes and stainless steel wires as discharge electrodes. The study was divided in two stages: the first studied the collection of PM2.5 (high risk particle matter), using alumina, with median diameter of 0.6 mm and maximum diameter of 2.5 mm as test powder, while the stage 2 focused the nanometric range, utilizing particles of sodium chloride, with diameters between 8 and 100 nm. In stage 1, the project parameters analyzed were duct length (L), diameter of the discharge electrodes (2r) and spacing between them (2c); the operational variables were the electric field (E) and the air velocity (v). Each variable was evaluated in two levels, whose values were Lne = 15 and 30 cm; 2r = 0.045 and 0.025 cm; 2c = 4 and 2 cm; E = 5 and 8 kV/cm and, finally, v = 0.5 and 1.0 m/s. The process performance was evaluated through the analysis of the global efficiency of removal of the particula te matter, obtained through isokinetic sampling. In stage 2, the project parameter analyzed were the duct length (Lne), the ratio s/2c and the distance between the collection plates (2s) and the operational variables were the air velocity (v) and current density (j). Each operation variable was evaluated in three levels, whose values were v = 0.125, 0.25 and 0.50 m/s and j = 1.8, 2.8 and 3.8 mA/m2. The project parameters were studied in two leve ls, Lne = 15 and 30 cm and 2s = 0.04 and 0.06 m. The process performance was evaluated through the analysis of the removal efficiency of the particulates obtained by particle counting, with the use of the Condensation Particle Counter 3007, from TSI. It was observed that the precipitator possesses high collection efficiency, even in the nanometric size range, were efficiencies larger than 99% were obtained, depending on the used configuration. As expected, the increase in air velocity, as well as the decrease of the collecting plate length and of the generated current caused a decrease in the efficiency. Conversely, larger spacing between plates resulted in an increment in the efficiency, phenomenon also observed by some other researchers for larger particles. The ratio s/2c did not show a clear tendency in particle removal. When compared to models from the literature, the one that offered the better adjustment to the experimental data was the classic Deutsch model when used with a drag coefficient given by a modified version of the Li and Wang (2003a e b) equation.
Método baseado em processamento digital de imagens para diagnóstico precoce de micro-estruturas dentárias
(Universidade Federal de São Carlos, 2005-05-25)
Ohnishi, Yuji de Oliveira
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Cruvinel, Paulo Estevão
;
https://lattes.cnpq.br/7924553462118511
This work presents a new method based on digital image processing dedicated to establish diagnosis of dental microstructures. The system uses image techniques such as segmentation, Hough Transform for circular pattern recognition for dental microstructures, and Bayesian technique to classify patients that present different dental structures. Results show the qualification of the method. Also, the adoption of the DICOM standard has shown the suitability of the method to be used in clinical and specialized environments.
Os professores de matemática e a geometria: opiniões sobre a área e seu ensino.
(Universidade Federal de São Carlos, 2005-05-25)
Crescenti, Eliane Portalone
;
Tancredi, Regina Maria Simões Puccinelli
Based on the professional experience with the Geometry teaching and on the existing bibliography which is approaching the abandon of Geometry and its teaching, the possible causes of this abandon and the importance of its teaching as well as its ransom in the Mathematics classes appeared disquietude which originated the present research, of which objective constitutes in knowing how the Geometry teaching developed in the 5ths 8ths grades of the Elementary School (Ensino Fundamental) and what the Mathematics teachers thought about Geometry and its teaching. Being so, the focus of this research was teachers, professionals whose voices must be heard in order to understand the school reality better. The data collecting involved interviewing nine Mathematics teachers with different years of experience and the methodological foundation of our work was based on their narratives. Their narratives showed that they have some lack of autonomy as well as precarious knowledge of the importance of the Geometry contents. The justification of the more experienced teachers did not differ from the ones placed by the less experienced teachers, being the less experienced teachers very attached to the bureaucratic organization and basing themselves more on the didactic books. More emphasis in the Geometry teaching was noticed among the more experienced teachers and these teachers were more successful and more respected by the students, although their practices were closer to the traditional teaching. The resources used by two of the less experienced teachers were closer to the more modern pedagogical approaches. We have also noticed, in general, that the educational programs basic or continuing education have not backrested the educational practices with the Geometry teaching and that in the school there isn t support for this developing either. On the other hand, to work with narratives turned possible to know the teacher practice better, in order to indicate subsidies to the present situation of the Geometry teaching to improve that situation.
Avaliação do repertório verbal inicial em crianças com deficiência auditiva pré-lingual usuárias de implante colclear.
(Universidade Federal de São Carlos, 2005-05-29)
Gaia, Tatiana Francis
;
Souza, Deisy das Graças de
;
https://lattes.cnpq.br/4404800720856419
The cochlear implant is a device that allows deaf people to detect surrounding sounds, including the human speech, which are transformed in electric stimulation of the cochlea. This is just the starting point for those who were deprived of audition, especially if the deafness occurred before language acquisition (pre-lingual deafness). After the implant, the person needs to learn (or relearn) to listen, and this is an important research and intervention issue. The goal of this study was to describe the evolution of the listening behavior in pre-lingual implanted children, in a period of time correspondent to the first 26 months after the implant. The analysis consisted of word recognition, figure naming and echoic behavior. In the recognition task, for each dictated word the participants were required to identify the corresponding figure (between three possible figures simultaneously available). On the figure naming task, a figure was shown and the participants were required to pronounce its name. On the echoic behavior required that a dictated word should be repeated by the participants. Twenty words were tested on each evaluation for word recognition, and the same twenty words were tested on the two remaining tasks. A microcomputer presented the participants with the stimuli (so oral-facial clues were avoided), and recorded responses that required selection, spoken words by children were videotaped and transcribed by direct observation. Three successive evaluations were performed with a mean interval of 5 months between them. Pre-training was conducted in order to orient the children on how to do the tasks and use the mouse. The tasks were applied individually, and the data analysis was also performed separately for each participant. The initial evaluation showed intermediate scores on word recognition and low scores or no correct answers on figure naming and echoic behavior. Progressive and substantial increase in word recognition naming and echoic behaviors also improved, but the magnitude of progress was much smaller than that observed in word recognition. In addition to the correct/incorrect criterion of analysis, answers that were close to the vocal emission considered correct (partially correct answers) were also analyzed, and a distinction between wrong answers and no answers at all was made. The evaluation of the partially correct answers was important for the identification of a progressive improvement on the echoic behavior and figure naming performances in which, although the children didn t reach the absolute correct criterion, they showed a systematic progress and attested learning of these important behaviors, which are part of language and whose acquisition is dependent upon audition. A second important observation was the reduced occurrence of no answers in the initial evaluations and the absence of this category of responding in the last evaluation. The improvements observed in the children s behavior are important indictors of learning oral language (comprehension and intelligible speech) after the implant; in the other hand, the gaps observed in their performances strongly suggest the need for educational interventions that aim to accelerate the acquisition of the implant user s repertoires, as well as to refine them, in order to maximize the benefits provided by this device.
Uma heurística para a programação da produção de sistemas flexíveis de manufatura usando modelagem em redes de Petri
(Universidade Federal de São Carlos, 2005-05-30)
Maggio, Eduardo Gomes Ribeiro
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Morandin Júnior, Orides
;
https://lattes.cnpq.br/4192845106907956
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https://lattes.cnpq.br/4851579746092098
The Petri Net based Search has been shown as a promising way to solve Flexible manufacturing Systems (FMS) Scheduling Problem. However, the response time is critical since it s a system with high computational complexity. Focusing the reduction of response time, this work proposes a heuristic for Petri Net based Search to solve FMS Scheduling problem of makespan minimization. Experiments showed improvements on response time reduction comparing with prior works
Um método de análise de cenários para sequenciamento da produção usando lógica nebulosa
(Universidade Federal de São Carlos, 2005-05-31)
Silva, Allan Rodrigues da
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Morandin Júnior, Orides
;
https://lattes.cnpq.br/4192845106907956
Production planning in automated manufacturing environments is a complex task which comprehends, among other activities, the production sequencing. One of the techniques used to determine the best production sequencing is the simulation. All possible sequences can be simulated and, right after that, the sequences with the best performance are verified, according to some criteria. However, due to the combinatory nature of the sequencing, the simulation of all possible combinations takes long time, becoming quite impracticable in many cases. Besides, events that were not programmed occur frequently in automated manufacturing environments and the decision maker must be fast choosing alternatives. Aiming to contour this problem, this work proposes a fuzzy analyser of sceneries for the production sequencing (ANCSP). The goal is to reduce the quantity of sequences to be simulated by the decision maker when non programmed events occur in the productive system, according to a defined performance criteria. The maximum quantity of generated sequences is calculated based on the time avaliable to realize the simulation. The proposed model intends to support the usage of the simulation technique by the decision makers. The ANCSP was implemented and various tests were performed. A specific model of flexible manufacturing system was considered to perform the tests. The performance of the ANCSP was analyzed comparing its results with those presented by a simulation software, for the same test sets and in accordance to the performance measures established.
Extensibilidade de ambientes virtuais colaborativos através de estórias interativas não lineares
(Universidade Federal de São Carlos, 2005-05-31)
Duarte, Diego Daniel
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Araujo, Regina Borges de
;
https://lattes.cnpq.br/8964123297688432
;
https://lattes.cnpq.br/3770885649274622
3D Collaborative Virtual Environments (CVE) applications have been impelled mainly by the computer games industry, military and industrial training and product design development. However, most of the existing systems are focused on specific tasks and have a supporting structure, which is closely tight to the application. As a consequence, a change in the application usually means a change in the supporting structure too, making it difficult and expensive to develop new or to extend existing CVEs. This work presents a novel approach to building and extending CVEs through the integration of interactive non-linear stories and Virtual Reality. With this approach, CVEs applications are composed as non-linear stories which can be changed either completely or partly, making it easier for developers to build and/or extend CVEs applications, such as highly dynamic marketing campaigns to 3D virtual shops, customized training courses, educational and entertainment games, etc. To allow the description of the CVE through interactive non-linear stories was developed a description language based on XML, named VEML. The use of a VEML for building a CVE application can facilitate the communication among the different professionals involved in the building of complex VE applications.
AMGra: uma abordagem para migração gradativa de aplicações legadas
(Universidade Federal de São Carlos, 2005-05-31)
Fontanette, Valdirene
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Prado, Antonio Francisco do
;
https://lattes.cnpq.br/2668568143800755
Along the time the applications usually need to evolve to catch up with and to assist to the new technologies that appear or get mature in the different computing areas, as it happens, for instance, with the programming languages. Starting from that demand, new researches are being accomplished to offer support to this development. This situation was improved with the ripening of Web technologies, once the need of the company nowadays is not always to convert an application totally, but to adapt these applications to accommodate these new technologies. The modernization of old applications, denominated legacy applications, is an arduous and a high cost task for the companies. Although there are several approaches that support this process in the literature, there is still a lack of resources that offer flexibility, take advantage of previous investments of the companies and get practical results. This way, the modernization process in the companies is almost always accomplished in a manual and "ad-hoc" form. Based on these ideas, this work presents AMGraA, an approach for gradual modernization of legacy applications, reconstructing them to attend to new technologies that came out along the time. This research project resulted of a partnership among GOES (Group of Software Engineering), a software development company, and FAPESP in support to the research and the development in the small companies.
Sensibilidade comportamental cruzada entre anfetamina e nicotina induzida na adolescência persiste até a idade adulta
(Universidade Federal de São Carlos, 2005-06-06)
Santos, Gabriela Cristina dos
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Planeta, Cleopatra da Silva
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https://lattes.cnpq.br/2514762545280942
Behavioral sensitization is a progressive and enduring enhancement of the motor stimulant effects of psychostimulants and other drugs of abuse, elicited by repeated administration of these drugs. This phenomenon has been extensively studied in experimental animals and has been implicated as a key component in drug addiction. Nicotine and psychostimulants are often abused in combination. The aim of the present study was to examine the cross-sensitization between nicotine and amphetamine in rats exposed to repeated treatment with the drugs during adolescence. We evaluated the effects of the treatment with nicotine (0.4 mg/kg; s.c.) or amphetamine (5.0 mg/kg, i.p.) from postnatal day (P) 28 P34 on amphetamine- (1.0, 2.5, 5.0 mg/kg, i.p.) or nicotine induced locomotor activity 3 (P37) or 10 days (P44) after drug treatment ended. Further, the effects of amphetamine or nicotine were examined 36 days (P70) after nicotine or amphetamine treatments to determine whether the effects of drug exposure during periadolescence persisted into adulthood. Adolescent animals pretreated with amphetamine were sensitized to the locomotor-activating effects of nicotine. Moreover, the pretreatment with nicotine during adolescence increased locomotor activity induced by 5.0 mg/kg of amphetamine. Although adult rats are less sensitive to nicotine, this drug significantly increased the locomotor activity in adult rats that were pretreated with amphetamine during adolescence. In addition, in adult rats, the pretreatment with nicotine during adolescence increased locomotor activity induced by 1.0 mg/kg of amphetamine. These data suggest the crossensitization between amphetamine and nicotine in adolescent rats. Moreover, the sensitization is long-lasting and persists until adulthood.
Contribuição do uso de modelagem de processos de negócios na implementação de ERP de fornecedores nacionais em pequenas e médias empresas.
(Universidade Federal de São Carlos, 2005-06-06)
Silva, Fernanda Pereira Carneiro da
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Pereira, Neocles Alves
;
https://lattes.cnpq.br/3744216524256924
The purpose of this study is to investigate the implementation of Enterprise Resource Planning (ERP) in small and medium enterprises (SME) in whom it says respect to the Business Processes Modeling (BPM) use. Until little time behind, the SME were not using conventionals of system ERP. Observed the BPM use, for the suppliers, and verified it influence in the integration of the internal processes of the business of the customer. Due to the fact that the utilization of (ERP) in small and medium enterprises is a recent issue, most of the reviewed literature was found in articles and books addressed to big companies. Substantive matters on business processes modeling (BPM) were found in international articles and books. Hardly ever, BPM, ERP or SME issues were discussed in the same article or book. Therefore, for this inquiry, one searched the information in the suppliers and customers of ERP, by means of differentiated questionnaires, that had been applied in the national supplying greaters of systems and in some customers of the system of these suppliers. This research contributed with the systematization of theoretical information regarding the concepts of BPM and in the vision of the ERP sustem in SME. With the carried through research of field, it made possible to get real information of the main characteristics of implementation with the use of the BPM for the suppliers and of its interference in the internal processes of business of some using customers, showing indications of that the BPM contributes for a bigger integration of the internal processes of business and that this option is made by the customer and that all the suppliers of this research had conditions to take care of it.
Ensino e pesquisa no centro universitário o caso da UNORP
(Universidade Federal de São Carlos, 2005-06-07)
Corrêa, José Luiz Falotico
;
Marques, Waldemar
The present work aims to perform a study in the Universitary Institution, specially at Centro Universitário do Norte Paulista Unorp about the teaching and researching activities based on an explanatory research using a Case Study methodology. In order to obtain the data that is based on the performance analysis, using the bibliography and documental research to establish the theoretical reference that supports our work and our considerations. After this survey questionnaires were handled to 55 teachers from the universitary institution, which is the object of our work. In these matters we aimed to raise concepts which would be the basis of our analysis, that is the teaching excellence concept, the relations between teaching and research and the methodologies adopted in the class which may be considered as research activities. Another aspect presented is related to the experiences brought by the teacher about his academic and personal life, as well as our school life course is part of the work s first chapter. These elements support the explanations presented and lead us to understand better what kind of research is conducted in the universitary institutions and which place it occupies in the role of pedagogical and teaching activities that are responsible to major the students from different courses
Ictiofauna da microbacia do Córrego da Lapa, Bacia do Alto Paraná, Itirapina/Ipeúna, SP
(Universidade Federal de São Carlos, 2005-06-07)
Fragoso, Evelise Nunes
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Verani, Nelsy Fenerich
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https://lattes.cnpq.br/1862746100743391
;
https://lattes.cnpq.br/6561803596789261
Preparação e investigação das propriedades de filmes de polipirrol modificados com partículas metálicas magnéticas
(Universidade Federal de São Carlos, 2005-06-07)
Bergamaski, Franciele de Oliveira Freitas
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Souza, Ernesto Chaves Pereira de
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https://lattes.cnpq.br/1505400360366643
;
https://lattes.cnpq.br/1192013631012905
In this work, it was investigated the preparation and characterization of polypyrrole and nickel or cobalt doped popypyrrole. To prepare the doped samples, the monomer was polymerized in a solution containing the metal salt. The following preparation variables were investigated using the factorial design: synthesis charge, nickel salt concentration and synthesis potential or synthesis charge, synthesis potential and temperature for nickel and cobalt doped samples, respectvely. Besides, the surface response analysis was used to propose an empirical law to describe the amount of incorporated particles in the samples. The polymers were characterized using cyclic voltammetry and a new reduction current density peak was detected which was attributed to the reduction of the metallic particles. The quartz crystal microbalance allowed us to propose the existence of a redox pair between the polypyrrole and the nickel particles. The nickel doped film morphologies were characterized using the SEM technique and the results that the these parameters were affect by the presence of nickel salt during the sample preparation.
Ecologia de peixes que ocupam diferentes habitats da Planícies de inundação do rio Mogi-Guaçu, SP
(Universidade Federal de São Carlos, 2005-06-08)
Simabuku, Mara Adriana Marçal
;
Peret, Alberto Carvalho
;
https://lattes.cnpq.br/0995579236780271
Fish from the Mogi-Guaçu River floodplains were sampled mostly in the wet season of 2001 to 2004, using several sampling gears, such as gill nets, sieves and a Dip net. Sixty two species were collected, belonging to 5 orders and 23 families; Capture for Unit Efford (CPUE) data indicated the predominance of lentic species of small characids in all habitats, except in the temporary flooded areas where early juvenil and juveniles of facultative migratory species prevailed; in lower proportion juveniles and adults of migratory species occurred in oxbow lakes and backwaters, early juvenil in tributaries and larvae, early juvenil, and juveniles in flooded areas. Dissolved oxygen concentrations were low in small temporary pools and temporary flooded areas, varying in the oxbow lakes. The relative abundance of standard length classes of some species such as Prochilodus lineatus and three species of Astyanax evidenced the preference of lower population strata for temporary flooded areas of upper Mogi-Guaçu, whereas higher strata occurred in oxbow lakes; backwaters and tributaries are inhabited by all population strata in low abundance. Eggs, in high densities, were sampled by ichthyoplankton net and Dip net only in the main river and its backwaters downstream Cachoeira de Emas dam, in the medium-upper stretch of Mogi-Guaçu River, in January 2003 and 2004. Evaluation of the anthropic influence on the watershed, revealed that deepening of the river channel by sand excavation, drainage of floodplains and river flow control by hydroelectric dams are changing flood patterns, as well as pollution by domestic sewage is negatively influencing fish nurseries.
Pescadores e turistas em uma área marinha protegida: uma contribuição ao planejamento da Reserva Extrativista Marinha da Ponta do Corumbau, Bahia - Brasil
(Universidade Federal de São Carlos, 2005-06-10)
Stori, Fernanda Terra
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Nordi, Nivaldo
;
https://lattes.cnpq.br/8168865557947289
Marine-coastal areas in the South of Bahia State in Brazil has experimented environmental and social changes, related to the over-exploitation of the fishery stocks and to the overflow of carrying capacity of the bathing resorts. Most of traditional communities are migrating out forced by the oppressive tourism economy, leaving fishery and facing cultural and social conflicts. A Marine Protected Area called Ponta do Corumbau Marine Extractive Reserve (or Corumbau Resex) was created in the year 2000, with the aim of sustainability of artisanal fisheries against predatory industrial activity, and to protect a rich biodiversity. The present research has as main objective to contribute to the planning of tourism in this Marine Protected Area through identification of potentialities and conflicts, social and ecological, of tourism activity in the tree studied villages: Cumuruxatiba, Corumbau e Caraíva. There were applied 157 semi-structured questionnaires between tourists and extractivists (fishermen) allowing to investigate the perception of the region and its context in two points of view. The scenic beauty, an opportunity of resting and freedom as well as local receptivity were appointed as the main potentialities by the interviewed tourists. Speculation for land property was identified as motive of conflicts on social relations and extractive culture. Other problems, mostly related to the infrastructure from the villages were also identified. Most of the interviewed extrativists (45% in Cumuruxatiba, 55% in Corumbau and 85% in Caraíva) are nowadays involved with tourism activities. It was possible to note that an initial period of visitation was occurred in the end of seventies and the tourist activity on the tree villages was identified in transition between the Involvement stage to Development, according to Butler (1980). In the same way, according to Smith (1991), Corumbau was identified as Resort Established and Cumuruxatiba and Caraíva as Business Area Established. Such results shows the necessity to control the tourism growing and to an adequate planning of this activity, aiming to link conservation of natural resources, social and economic benefits to extractive communities and the valorization of the artisanal fishery system, the main reason of the Corumbau Resex creation.
O envolvimento do aluno no processo de ensino - aprendizagem durante a realização de atividades experimentais
(Universidade Federal de São Carlos, 2005-06-10)
Serafim Junior, Irineu Modes
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Ferreira, Luiz Henrique
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https://lattes.cnpq.br/4819009378941531
This work had as an objective to identify the motivation level of the students who belong to three public grade schools in the city of São Carlos with different socio-economical realities for the chemistry learning. Experimental activities were developed with the main objective of promoting the student s interaction with their friends and teachers. During the accomplishment of these experimental activities, it was also sought to arive the student s alternative conceptions of those who participated in the research, once this class type is quite favorable for discussions and spontaneous manifestations that has as a base the previous knowledge regarding the phenomenon in observation. At the end of the offered mini-courses, the students were invited to act as voluntary monitors in the chemistry classes in their own school. It was observed that the number of monitors that participated in the project, in relation to the total number of students, was very small in the outlying school and in the schools of central location. When compared by their shifts, the smallest number of participants was of students of the night period, probably due to the fact of they are hard-working students, in their majority. Establishing a comparison among the students from the different schools, a percentile adult of the students of the central schools participation was observed, once these students seem to be more motivated for learning. In the three schools, the monitorship activity was shown to be a motivation factor for the learning. All the monitors wrapped up in the project and they really helped in the laboratory classes, playing an active part in the teaching-learning process. Differences were also verified in the students of the three public schools profile, what signals for the importance of accomplishing more consistent studies on the profile of the schools before the definition of public politics for the teaching.
Aspectos metabólicos, fisiológicos e taxonômicos de actinomiceto produtor de exopolissacarídeos, proveniente de águas subterrâneas contaminadas (São Carlos, SP)
(Universidade Federal de São Carlos, 2005-06-10)
Fusconi, Roberta
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Godinho, Mirna Januária Leal
;
https://lattes.cnpq.br/4446317622651166
Strains Lc (CCT 7277) and Lg (CCT 7137), isolated from landfill leachate-contaminated groundwater and found to produce exopolysaccharides (ESP), were characterized by polyphasic taxonomy. The growth and EPS production of Lg strain on sugarcane molasses, at concentrations between 2% and 10% and on the standard GYM medium were studied and its responses to nutrient starvation were analysed. Morphological, biochemical, chemotaxonomic and molecular tests indicated that Lc and Lg have properties typical of Gordonia polyisoprenivorans DSM 44303T. Viable cell counts revealed that GYM medium was the best for growth (µmax = 0,232 h-1). EPS production started during the exponential phase and continued during the stationary phase, in all culture media. After 24h of stationary phase, G. polyisoprenivorans CCT 7137 showed a highest total EPS production in 6% (172,86 g/l) and 10% (139,47 g/l) sugarcane molasses media, a highest EPS production for unit of cellular concentration in 10% sugarcane molasses medium (39,03 x 10- 11g/UFC) and a highest porcentage of free EPS in 2%, 6% e 10% sugarcane molasses media (88,37%, 62,4% e 64,22%, respectively). G. polyisoprenivorans CCT 7137 showed three responses to nutrient starvation: a decrease in the cell viability (13,7% and 3,25% after 7 and 56 days respectively), a reduction in cell size (from 0,55 to 0,33 µm3) and a cell shape change (from rod to coccobacillus). G. polyisoprenivorans CCT 7277 and CCT 7137 are two of six strains of G. polyisoprenivorans so far isolated and recorded in the literature, and the first strains found in a brazilian ecosystem. The data presented here suggest the potencial of G. polyisoprenivorans CCT7137 to be used as ultramicrobacteria in porous media experiments such as to aim for the study of sugarcane molasses as nutrient source for in situ EPS to biorremediate contaminated sites through the biobarrier technology.
Estudos funcionais e estruturais da proteína recombinante humana UBE2G2 (Ubiquitin-conjugating enzyme E2G2)
(Universidade Federal de São Carlos, 2005-06-10)
Reyes, Luis Fernando
;
Silva, Flávio Henrique da
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https://lattes.cnpq.br/1757309852446263
The ubiquitin system represents a selective mechanism for intracellular proteolysis in eukaryotic cells that involves the sequential activity of three enzymes, E1 (Ubiquitin activating enzyme), E2 (Ubiquitin-conjugating enzyme), and E3 (Ubiquitin-protein ligase). The identification of these proteins and their targets as well as structural data is essential to understand their function in the eukaryotic cell. In the present study the open reading frame of human Ubiquitin- conjugating enzyme UBE2G2 was isolated from a human brain cDNA panel, cloned into pET28 vector and expressed in Escherichia coli. His-tagged protein was then purified by nickel-affinity chromatography and subjected to structural and functional studies using circular dichroism (CD) and an in vitro ubiquitin-binding assay, respectively. The affinity chromatography assay rendered approximately the 27 mg of the soluble recombinant HisUBE2G2 after expressed in bacteria at low amounts of IPTG (0,1mM) in 3 hours of induction. The CD spectra of recombinant pure protein showed a secondary structure content according with the expected for a member of the E2 family (Ubiquitin-conjugating enzyme), with 35 % of α-Helix, 21 % of β sheets and 23 % of turns. Moreover, purified protein was able to bind ubiquitin molecules when mixed with a HeLa cell extract during the pull-down assay. Taken together the results presented in this work allow inferring that HisUBE2G2 was expressed in their active form.