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Estudo do efeito da Alternagina-C, uma desintegrina do veneno de Bothrops alternatus e de um peptídeo sintético derivado de sua estrutura, sobre a expressão de fatores de crescimento, angiogênese e cicatrização de lesão em pele de rato
(Universidade Federal de São Carlos, 2008-05-07) Sant'Ana, Estela Maria Correia; Araújo, Heloísa Sobreiro Selistre de; https://lattes.cnpq.br/8749163000663076
Angiogenesis and wound repair are mediated by several growth factors that are strictly released. Alternagin-C (ALT-C), a disintegrin-like protein from the venom of Bothrops alternatus induces in vivo angiogenesis as well as the cyclic peptide derived from its primary structure, with the ECD motif (ALT-C PEP). This study investigated the effects of ALT-C and ALT-C PEP on angiogenesis and expression of growth factors in a model of wounded rat skin. The rats were anaesthetized; one cutaneous excision (4 mm diameter) was made on the back of each animal, close to the cervical area. Animals were then divided into 7 groups (five rats/group): control (treated with vehicle); locally treated with 10, 60 and 100 ng ALT-C or 10, 20 and 100 ng ALT-C PEP ALT-C PEP for 1, 3, 5 or 7 consecutive days. At the end of experiments animals were killed, the skin was removed; the cranial portion was used for histological analysis and from the caudal portion; protein were extracted, separated by SDS-PAGE and VEGF, FGF-1, TGFβ1, PDGF, IGF1 e TGFα expression was analyze a by Western blotting. The results show that both, ALT-C and ALT-C PEP induced the formation of new vessels, stimulate collagen synthesis and modulated the expression of growth factors, mainly VEGF and FGF1. The increase in VEGF expression could be detected up to 7 days after injury. FGF1 was also significantly increased, but at a lesser extension than VEGF. In conclusion, we present a protein belonging to the disintegrin group of snake venom toxins stimulates angiogenesis and collagen synthesis in an injured tissue. ALT-C may exert its effects via integrin binding and growth factor modulation. As angiogenesis is an important event for wound healing, ALT-C and ALT-C PEP could be candidates to the development of a new drug for wounded skin repair or regeneration.
A construção da gestão compartilhada da reserva extrativista do Mandira, Cananéia, SP
(Universidade Federal de São Carlos, 2008-05-07) Cardoso, Thaís Almeida; Nordi, Nivaldo; https://lattes.cnpq.br/8168865557947289; https://lattes.cnpq.br/1975625195000271
This study arose from the proposal of fisheries co-management, based on the sharing of decisions and responsibilities between the State, user groups and other local organizations. The research was developed in the Mandira Extractive Reserve, Cananéia, SP, Brazil, with the objective of contextualizing the way of life and use of resources by the beneficiaries and, using the Research-action, to build up the co-management of the Protect Area. The process was developed by the collective action, of a group of local residents and technicians, looking for the involvement of the other beneficiaries for the effective participation in the management of the Mandira Extractive Reserve. We took as a basis for process analysis the management of the oyster (Crassostrea brasiliana), the main resource used by the local residents, which was frightened by super-exploitation. From the structure of the co-management mechanisms in the Protect Area and of the formulation of simple rules, created in a participative way and contextualized to the local reality, we obtained important results, which involved since the empowerment of the community, in search of autonomy and legitimacy, to the recovery of the stock of the oyster in the Reserve. It is important to emphasize that for the consolidation of this process, making it effective along the time and applicable to other fishing resources used inside the Mandira Extractive Reserve, the strengthening of the local organizations, as well as the implementation of other monitoring mechanisms, with the support of the administrating agency, adopting them as public police, should be maintained.
Efeito do laser de baixa intensidade sobre a regeneração do músculo esquelético em camundongos através da análise de expressão gênica com branched de DNA(bDNA)1
(Universidade Federal de São Carlos, 2008-05-07) Brasileiro, Olga Sueli Moreira; Parizotto, Nivaldo Antonio; https://lattes.cnpq.br/5527703735628408