Browsing by Issue Date, starting with "2009-04-22"

Filter results by year or month

Now showing 1 - 20 of 20923

Significado social e sentido pessoal da atividade docente do professor de inglês da escola pública
(Universidade Federal de São Carlos, 2009-04-22)
Ianuskiewtz, Andréia Dias
;
Viana, Nelson
;
https://lattes.cnpq.br/6252579012192711
;
https://lattes.cnpq.br/8530008627127423
This research aims to investigate the social meaning of the public school English teacher s work and the personal sense he/she attributes to his/her teaching activity. To achieve this objective we used as theoretical basis the sociohistorical approach and the Activity Theory which was developed by Leontiev (1978; 2004). In order to understand the social meaning that is associated to the English teacher s activity we surveyed authors who deal with relevant issues about language learning and teaching (ALMEIDA FILHO, 1993, 2003; MOITA LOPES, 1996, 2003, 2008; PAIVA, 2003, 2008; CELANI, 2005; LEFFA, 1999, 2003; PENNYCOOK, 2008, among others) and we also reviewed official documents for the Brazilian educational public politics (Parameters, Orientations and National Curricular Proposals for the teaching of Foreign Languages). The research methodology involved the application of a questionnaire to twenty-six English teachers of public schools from a countryside city of the state of São Paulo. With the help of this instrument, we gathered data that allowed us to outline the present profile of those teachers and to obtain information on several aspects involved in their daily work. We aim to answer the following questions: (1) Which social meanings are attributed to the activity of the public school English teacher? (2) Which personal sense the public school English teacher attributes to his/her work? and (3) Which relation can be established between the social meaning and the personal sense related to the teaching activity? By exploring the multiple variables involved in the public school English teacher s work and by verifying the meanings and senses associated to his/her teaching activity, this study may contribute to a better understanding of the conditions that involve that activity, by allowing the problematization of values and attitudes that guide and ground the teaching of English in the context of public schools.
Avaliação do efeito do inóculo e do perfil de alimentação do mosto na produção em escala piloto e industrial de etanol
(Universidade Federal de São Carlos, 2009-04-22)
Martins, César Augusto Porto
;
Cruz, Antonio José Gonçalves da
;
https://lattes.cnpq.br/5480542225280883
Terminologia da indústria de artefatos de borracha: proposta de um vocabulário
(Universidade Federal de São Carlos, 2009-04-24)
Bazzon, Solange Cristina Maida
;
Almeida, Gladis Maria de Barcellos
;
https://lattes.cnpq.br/4046789388750478
;
https://lattes.cnpq.br/3722453885049927
The rubber industry is a sector that contributes significantly with the national economy due to the fact that it is part of a wide range of products. Therefore, we verified the need of a terminological systematization in this field. Based on this fact, our objective is to elaborate a monolingual vocabulary (glossary) of rubber goods in the automotive sector, with English equivalences. This paper is supported by the Communicative Terminology Theory proposed by Maria Tereza Cabré. Under a communicative perspective, the methodology of this research contemplated the compilation of a corpus as well as the utilization of a computer tool, the Unitex, in order to manage the texts that make part of this corpus. We extracted words (semi automatically) which we considered a term candidate, inserted them in the ontology, elaborated and filled out the terminology forms which were used as a term dossier; elaborated a text definition basis which was constantly fed with new information and sequentially we wrote the definitions. The definition texts of the rubber vocabulary (glossary) are based on the researches at Terminology Group Studies department GETerm. The microstructure - headword - contemplates systematic (obligatory) and asystematic (non obligatory) information that facilitates the peoples use. Concerning the vocabulary/ glossary microstructure we followed the TERMICAT s (1990) proposal, which aims the specialized communication.
Ecologia reprodutiva de Sterna hirundinacea Lesson, 1831 e Thalasseus sandvicensis (Lathan, 1787) (Aves, Sternidae) na ilha dos Cardos, Santa Catarina, Brasil
(Universidade Federal de São Carlos, 2009-04-24)
Fracasso, Hélio Augusto Alves
;
Verani, José Roberto
;
https://lattes.cnpq.br/0053232138684810
;
https://lattes.cnpq.br/6981162649888529
Despite being widely distributed in South America, the South American Terns (Tierra del Fuego to Bahia and all around the Pacific coast to Peru), and Cayenne Terns, (from the Caribbean Islands to the region of Deseado Port), and having relatively large populations, little is known about the ecology of these species in the Neotropical region. The present study is a contribution to the knowledge of the reproduction and trophic ecology aspects. Samples were taken daily on the Cardos island, SC, Brazil, along 2003, 2005, from 2006 to 2008, aiming to characterize the reproductive ecology of these species. The season of 2003 began with the arrival of the first adults in mid-May, and in 2005 and 2006 occurred in late April, with the departure of adults in early October. 2486 nests of S. hirundinacea were recorded in 2005 to 1,852 in 2006, with average clutch size ranged from 1.45 in 2006 to 1.19 in 2003 and the hatching success in 2006 was estimated at 82.16%. While for Thalasseus sandvicensis in 2003, it took on average 23.5±0.2 days for the hatching of chicks, and success of 83.33%. Was recorded 6,248, 5,140 and 4,006 preys transported by S. hirundinacea and 1157, 628 and 98 by Thalasseus sandvicensis for the colony during seasons of 2003, 2005 and 2006, respectively. The delivery of food to the chicks occurred since the early hours of the morning until late afternoon. The first chicks hatched in July 2003 and June 2005 and 2006, with reproductive success for S. hirundinacea of 50.94%, 35.96 and 53.47%, and Thalasseus sandvicensis of 48.00, 29.94 and 5.00%, respectively. The highest growth rate for S. hirundinacea occurred in the first 20 days of life with small increments until the flight and subsequent abandonment of the island around 46 days in 2003, 45 in 2005 and 42 in 2006. The small size of the island, the human low pressure and little activity of the gull (Larus dominicanus), considered the main predator on the Brazilian coast, during the egg laying period, allowed the regular occupation, making it an important site for the conservation of these species.
Modelagem do planejamento agregado da produção em usinas cooperadas do setor sucroenergético utilizando programação matemática e otimização robusta
(Universidade Federal de São Carlos, 2009-04-24)
Paiva, Rafael Piatti Oiticica de
;
Morabito Neto, Reinaldo
The main concern of this work is related to the development of an aggregate production planning model of a cooperative society of sugar and alcohol milling companies. This mathematical model is based on a hierarquical approach between the annual planning problem of the cooperative and the tactical planning horizon of the sugarcane mills. In the cooperative level the main questions are related to the allocation of production goals to each mill and the management of inventory and dynamic demands. In the milling companies level a process selection model aims at helping the decision makers to determine the quantity of sugarcane crushed, the selection of sugarcane suppliers, the selection of sugarcane transport system suppliers, the selection of industrial process used in the sugar, alcohol, molasses and energy production. Besides that, this work presents an analysis of the impact of uncertainties in the aggregate planning problem parameters, using robust optimization techniques. To solve the linear and mixed integer mathematical problem found in this modeling, we applied a state of the art modelling language with an optimization solver. A case study was developed in a cooperative of sugar and alcohol milling companies located in the state of Alagoas- Brazil and at Santa Clotilde mill, located in the city of Rio Largo-AL. The results of this case study helped us to verify the applicability of the proposed models in the aggregate production planning of the studied organizations. Computational results are presented and analyzed with real data application.
Avaliação do papel dos receptores 5-HT3 da substância cinzenta periaquedutal de camundongos submetidos ao labirinto em cruz elevado
(Universidade Federal de São Carlos, 2009-04-24)
Silva, Luana Tenório da
;
Souza, Azair Liane Matos do Canto de
;
https://lattes.cnpq.br/2352004564367849
;
https://lattes.cnpq.br/2915992384961428
The exposure of animals to aversive situations, such as elevated plus-maze (EPM), activates serotonergic pathways with projections into structures involved in the defense system, such as the amygdala, septum, hypothalamus, hippocampus and periaqueductal grey matter (PAG), producing behavioral changes that can be characterized as anxiety. However the serotonin (5-HT) presents a dual role in this modulation. Thus, while the stimulation of the receptor subtype 5-HT1A and 5-HT2 prosencephalic in structures such as amygdala and hippocampus result in potentiation of responses of anxiety in rodents, the activation of these receptors in the PAG, often tends to reduce behaviors related to anxiety . This study focused the role of 5-HT3 receptors in the PAG in the anxiety in the mice EPM test. In experiments 1 and 2, mice received infusions intra-PAG of ondansetron (0, 0.3, 1.0, 3.0 nmol/0.1 μL) and mCPBG (0, 40, 80 and 160 nmol/0.1 μL), 5-HT3 receptors antagonist and agonist, respectively. As the mCPBG not changed any of the conventional indices (% open-arm entries and % open-arm time) and risk assessment, we investigated in experiment 3 the possibility of interaction between 5-HT3 and 5-HT2 receptors. For this, we perform combined microinfusions of intra-PAG ondansetron and mCPP, an agonist of 5-HT2B/2C receptors, on behavior of maze-naïve mice. The results showed that intra-PAG infusions of ondansetron (3.0 nmol) increased the behavioral indices of anxiety. None of the doses of intra-PAG infusions of mCPBG modified the conventional and ethological indices of anxiety. The anxiolytic-like effect produced by intra-PAG infusions of mCPP (0.03 nmol) was blocked by infusions of ondansetron (1.0 nmol) in the same mesencephalic structure. All effects were observed in the absence of significant changes in locomotor activity (closed-arm entries). Our results indicate that there is a possible interaction between 5-HT3 and 5-HT2B/2C receptors modulation into the PAG of anxiety in mice.
Aspectos semânticos da definição terminológica (DT): descrição linguística e proposta de sistematização
(Universidade Federal de São Carlos, 2009-04-27)
Kamikawachi, Dayse Simon Landim
;
Almeida, Gladis Maria de Barcellos
;
https://lattes.cnpq.br/4046789388750478
The making of the definitions is the most complex step in terminological research which aims to compile specialized dictionaries, for the good ones are evaluated mainly as to the quality of their definitions. As there are differences between terms and words, there are also differences between defining them. While the lexicographic definition specifies and describes the meanings of words as language units, giving all their possible senses, the terminological definition (TD) identifies and describes terms only in reference to the specific conceptual system to which they belong. Therefore, the TD is the object of this Master s study. Our undergraduate experience in terminological research in the Group of Studies and Research in Terminology (GETerm), revealed several difficulties associated with the TD process. The defining method was changed, adapted, extended, and revised to handle the challenges of the research. From that experience, we came to a set of methodological procedures to aid the TD routines in any domain knowledge. Accordingly, this Master s dissertation research aims to systematize those procedures to be implemented into a Web-based, computational environment (E-Termos) to aid research groups in their terminological endeavor.
Análise das estratégias, condições e obstáculos para implantação de técnicas mais sustentáveis para tratamento local de efluentes sanitários residenciais. Caso: assentamento rural Sepé-Tiaraju, Serra Azul-SP
(Universidade Federal de São Carlos, 2009-04-27)
Martinetti, Thaís Helena
;
Teixeira, Bernardo Arantes do Nascimento
;
https://lattes.cnpq.br/1166854416745527
;
https://lattes.cnpq.br/0321765590670438
The lack of sanitation systems is one of the main causes of insalubrity and environmental degradation. The term sustainability has provoked a debate in the literature, derived, mainly with the environmental concern and the use of the non-endless natural resources. Besides environmental and ecological issues, the concept incorporates the economical, social, cultural and political dimensions. The last one emphasizes the people participation on making decisions, management and general control of the production process, through the comprehension of the reality and the analysis of different alternatives. The use of participatory processes for taking decision to the choice of more sustainable domestic wastewater system, besides easing the knowledge transfer, permit access to the nonconventional techniques. General purpose was to analyze the strategies, conditions and obstacles for the implantation of more sustainable domestic wastewater systems, containing as experimental object the Rural Settlement Sepé-Tiaraju, located in Serra Azul-SP (Brasil), considering the participatory process for families taking decisions. General strategy used was the participatory action research, which the researcher and the families interact with each other in a cooperative and participatory way. General steps of the action were: 1- participatory process for domestic wastewater systems choice; 2- executive project and the budget system; 3- execution plan of the pilot system; 4- family organization for the execution of the pilot system; 5- the execution of the pilot system; 6- the system maintenance and utilization instructions. For each verified hypothesis, it was elaborated a spreadsheet in where it was explained: the type of information (variable), the evidence sources, the proceedings for the data collection and analysis. It was used as evidence sources the reports, the spreadsheets, the audiovisual images from the discussion meetings with the families and the execution of the domestic pilot wastewater system. Products were: 1- characterization of the domestic wastewater system choice by participatory process for the case of rural settlement Sepé- Tiaraju; 2- characterization of the domestic pilot wastewater system execution by participatory process; 3- sustainability and the participatory action research analysis for the more sustainable domestic wastewater system implantation. Results indicate that the use of participatory process enables the information access, the choice of non-conventional techniques and more sustainable, although it takes more time of the evolved actors (researcher and families) and the adequacy of the activities to the social reality and the capacity of comprehension of the participants. Challenges are related to the actors governance, conflicts and financial resources governance, besides the match of disposable time for the research and the action in the social reality.
Niilismo, transvaloração e redenção na Filosofia de Nietzsche
(Universidade Federal de São Carlos, 2009-04-27)
Barbosa, Ildenilson Meireles
;
Baioni, José Eduardo Marques
;
https://lattes.cnpq.br/9613180348313909
;
https://lattes.cnpq.br/2624665531049867
Many are the productions already developed about nihilism in Nietzsche s work, wich the perspective of unconditional affirmation of the world and life is assumed as an inevitable consequence on the overcomig of nihilism itself. However, even admiting that Nietzsche s thougth about nihilism conducts him to an unconditional affirmation of every happening, our research is developed by the nitzschean notion of redemption. Since The Birth of Tragedy, Nietzsche explores the theme in the reconciliation fullfilled by the tragical art between the apollonian and dionisian.In his work of 1872, the philosopher finds in Dionysus shapes a god that affirms the suffering, wich is a crucial subject to the future guiding of his supreme-affirmative thought. In contrast to the tragical conception that was formed as a way to escape from the pessimism, the socratism-platonism is understood by Nietzsche as a movement that puts in progress the nihilist logical in the West, consummated on the christian conception of negativity. In front of a terrible diagnosis presented by Nietzsche in his works of maturity, we ve try to show in what measure on one hand, answering to the question about provenience of nihilism, in the other hand, explicitating it s developing on moderness, Nietzsche s philosophy is able to think radically on the overcomig of nihilism, reaching expressively in the project of transvaluation with the same notion of human s redemption.
As delegadas de polícia de São Paulo: profissão e gênero
(Universidade Federal de São Carlos, 2009-04-28)
Silveira, Maria Natália Barboza da
;
Bonelli, Maria da Glória
;
https://lattes.cnpq.br/0051682015787141
The central objective of the research was to study the presence of women in legal careers working as a district chief of police, which is traditionally a male profession. For the development of this research were used two sources of analysis. The objective of the first was to examine women's participation of the profession through the statistics on this professional group. The objective of the second was to evaluate the Women s Police Stations to enable people to verify if the market reserve established by this institution contributed to a process of subordination of these professionals in comparison to their peers. The study made it possible to find out that, on one hand, the inauguration of the DDM's (Delegacias de Defesa da Mulher) channeled more women to work in these stations, on the other, contributed to an "imprisonment" of their career. This is because, for many chiefs of police, working in a DDM is considered an obstacle for the career. We also examined the social representations that the DDM developed at the corporation's police and within the profession of delegate of police, and what has changed in the structure and image of those police stations for the police profession. The study was conducted in São Paulo, where police districts were studied and DDM s. The study was conducted from 2007 until 2009. As a research methodology, interviews were done with chiefs of police from DDM s and police districts in order to establish a comparison between these organs. Altogether 12 interviews were conducted, and with 9 women and 3 with men.
Determinante do voto partidário: uma análise do voto em eleições para o Executivo no Estado de São Paulo – 1998-2006
(Universidade Federal de São Carlos, 2009-04-28)
Tavoni, Fabrizio Martins
;
Braga, Maria do Socorro Sousa
;
https://lattes.cnpq.br/2311094074614215
;
https://lattes.cnpq.br/7053353604209470
The objective of this work is to analyze the partisan vote in elections for the Executive in the State of São Paulo, as much with the vote for Governor how much for President. The clipping that was made for this work is the period that understands the elections of 1998, 2002 and 2006, being that the analyzed parties are: PSDB (Brazilian Social democracy Party) and PT (Labor Party). The studied data had been collected in TSE (Electoral Superior Court) e, from them, made crossings between candidate the President for a party and candidate the Governor for the other party and vice versa, in order to observe the coherence of the vote in the parties in distinct disputes. From this maps of the vote in the State was made, having shown where each party gets better resulted and also to verify if in such a way in the dispute for the government of the State how much for the Presidency it has coherence in the partisan vote.
A trajetória política tenentista enquanto processo: do Forte de Copacabana ao Clube 3 de Outubro (1922-1932)
(Universidade Federal de São Carlos, 2009-04-28)
Viviani, Fabrícia Carla
;
Cepêda, Vera Alves
;
https://lattes.cnpq.br/4917331423373631
;
https://lattes.cnpq.br/5015093424728336
This work analyzes the Tenentismo (lieutenants´ movement) ´s political trajectory from 1922, date of its appearance, to 1932, moment when the October 3rd Club created the Sketch for the Social and Political Reconstruction of Brazil. Its central aim is to detect how, during this period, the Tenentismo made a transit from a military group, defending institutional interests, to a political group, formulating a project for the society as a whole, that is, it changed from a reacting to a proposing movement. That being so, the hypothesis here considered is that the construction of the movement as a political actor coincided with its manifestations in the national scenery between 1922 and 1932. Supposedly, this transformation happened due to the fact that the lieutenants emerged from a historical moment of modernization of the Brazilian society and of the Armed Forces, and the interaction with this scenario forged its collective identity, and made it capable of interfering with the process of social change. For this purpose, starting from the idea that the Tenentismo was under construction, we will analyze the documents produced during this period by both the São Paulo and the Rio de Janeiro segments. Thus, the methodology employed is the primary analysis of Tenentismo´s manifestos, programs, handouts and letters from the period, since we believe the texts produced by these actors are the intellectual product of reflections, formulations, re-significations that oriented them towards action.
Influência de defeitos nas propriedades mecânicas de juntas soldadas por FSW da liga AA6013-T6
(Universidade Federal de São Carlos, 2009-04-28)
Marconato, Eduardo Amado
;
Alcântara, Nelson Guedes de
;
https://lattes.cnpq.br/3123107570992184
;
https://lattes.cnpq.br/8675013826737290
In 1991, it was developed a solid state welding process called Friction Stir Welding - FSW, intended to increase the number of applications for the aluminum and its alloys. As every welding process the presence of defects should be avoided to not compromise the quality of the welded joint. Some typical defects originating from FSW process are found in some joints depending of the welding parameters. Thus, this study has the purpose to evaluate the quality of welded joints in 3 mm thick 6013-T6 friction stir welded joints in function of the obtained defects, using different welding parameters. The characterization was based on metallography by OM and SEM of the welded joints in order to analyze the obtained defects. The mechanical properties were evaluated by tensile, microhardness and fatigue tests.Welds joints were intentionally produced to contain inner defects in the weld nugget like tunnel shaped along the joint and lack of root penetration of the welding tool. All welded conditions, the microhardness profiles show a big drop in hardness when crossing from the HAZ zone into the center of each weld, while the tensile and fatigue tests showed a strong defects influence in the mechanical properties. The lack of penetration weld defect had the worst results of mechanical properties compared to tunneling defects, and the sound weld joint exhibited the best results of mechanical properties for all conditions investigated.
Comparação da diversidade microbiana intestinal em larvas do campo e laboratório do bicudo da cana-de-açúcar, Sphenophorus levis (Coleoptera, Cucurlionidae)
(Universidade Federal de São Carlos, 2009-04-28)
Rinke, Raquel
;
Silva, Flávio Henrique da
;
https://lattes.cnpq.br/1757309852446263
;
https://lattes.cnpq.br/4245978323080124
The sugarcane weevil, Sphenophorus levis, is an important pest in sugarcane culture in São Paulo state, Brazil. To complete its life cycle, S. levis may depends on microorganisms that inhabit its intestinal tract and play an important key in the insect physiology and nutrition. In this study we report the characterization of the intestinal microbiota from population of insect larvae from field and laboratory. Analysis of 16S rDNA sequences revealed a total of fourteen genera, one group from Candidatus category and two uncultivable groups represented by Alfa-Proteobacteria, Beta-Proteobacteria, Gamma-Proteobacteria, Firmicutes and Bacteroidetes phylum. Microorganisms isolated through culture-dependent methods were classified according morphological parameters and using 16S rDNA molecular marker. In addition to bacteria, four filamentous fungi were isolated. It was observed a slightly higher bacterial diversity in field than in laboratory according to Shannon index (Field H'= 3,36; Laboratory H'= 3,26). It is also our objective in this work to search for microorganisms capable to degrade cellulose, an important event in the insect attack. From the cultivable microorganisms, five genera of bacteria and two filamentous fungi presented cellulolytic activity. This is the first study about S. levis microbiota which may contribute to understand the interaction plant-pathogen and also be useful for future development of new strategies for control of S. levis in sugarcane cultivation.
Tutoria instrucional centrada na leitura de livros em escolares com distúrbio de aprendizagem e dislexia do desenvolvimento
(Universidade Federal de São Carlos, 2009-04-29)
Machado, Andrea Carla
;
Rose, Tânia Maria Santana de
;
https://lattes.cnpq.br/7978344886545493
The aim of this study was to evaluate the effects of instructional tutorship focused on book reading in students with developmental dyslexia and learning disability based on the proposals of Clay s Reading Recovery Program (1993). Among the specific objectives, the study aimed at characterizing the performance in percentage and identify the strategies related to reading and writing found in pre and post-testing of all groups involved in the research (with and without intervention) and compare, in pre and post-testing, the results of students with developmental dyslexia and learning disability. A group of twelve students participated in the study, and group I (DL) was subdivided into: GDL1 composed of three students with developmental dyslexia that participated in the intervention and GDL2 consisting of three students with developmental dyslexia that did not participate in the intervention. Group II (DA) was subdivided into GDA1 consisting of three students with learning disability that participated in the intervention and GDA2 consisting of three students with learning disability that did not participate in the intervention. The research presented the design: pretesting, tutorship, post-testing. The data were collected in sessions held in pre and post-testing with tasks of the Diagnostic Survey (Clay, 1993). The interventions, based on instructional tutorship, were performed in twelve individual sessions, once a week, lasting one hour each during the first semester of 2008. The results were analyzed in a descriptive way by the score in percentage of correct answers. Positive effects were verified on the teaching of strategies and the study also provides strong evidence that instructional tutorship focused on book reading gives students with developmental dyslexia and learning disabilities benefits with significant differences shown by the development of students who participated in interventions. However, the results of this study revealed that there was an improvement in the tasks that were observed and strategies that were trained in students with a learning disability when compared with students with developmental dyslexia.
Aspectos da dinâmica populacional e reprodutiva de Pimelodus maculatus (Siluriformes, Pimelodidae) no reservatório de Cachoeira Dourada (GO-MG)
(Universidade Federal de São Carlos, 2009-04-29)
Sabinson, Lia Mayrink
;
Peret, Alberto Carvalho
;
https://lattes.cnpq.br/0995579236780271
;
https://lattes.cnpq.br/8836864670457648
Growth and reproduction parameters of the yellow-mandi, Pimelodus maculatus (LACÉPÈDE, 1803) (Siluriformes, Pimelodidae), were determined for the Cachoeira Dourada reservoir (GO-MG). The field work occurred throughout February 2007 to January 2008 (with the exception of December 2007). Gill nets with mesh sizes from 1, 5 to 10 centimeters were placed in three different areas in the reservoir and were collected 24 hours later. A total of 538 specimens were captured, amongst which 242 were females, 219 males and 77 could not have their gender determined. Sex ratio differed from 1:1 only during July 2007 and January 2008, with males and females predominating in each of those months. Males occupied the medium length classes (18,9 to 24,3 cm) while females were most abundant in the superior classes (from 27 to 37,8 cm).The L50 for males were 24,8 cm and 26,2 cm for females. The growth constant K assumed a higher value for females (K=0,1356) than for males (K=0,0383), revealing a much faster growth rate for the females of P. maculates, which may be due to their greater investment in reproductive tissue, a fact also indicated by the elevated values of Kn and GSI during the summer. Having in mind that P. maculatus reproduces in the rainy season, a greater gain in weight is expected during the months before the reproduction season, and that after it occurs the fish loses fat and weight as a consequence of metabolic effort. The reduction in the ecosystems complexity is consequence of the reduction in the surrounding diversity, and it seems to be the main factor modifying the population`s and reproductive dynamics of Pimelodus maculatus in the Cachoeira Dourada reservoir.
Produção recombinante e estudos funcionais de uma cisteíno peptidase de Xanthomonas citri subspécie citri
(Universidade Federal de São Carlos, 2009-04-29)
Silveira, Rosseli Santos da
;
Silva, Flávio Henrique da
;
https://lattes.cnpq.br/1757309852446263
;
https://lattes.cnpq.br/9963382495354916
The citrus canker disease is caused by a phytopathogenic bacterium Xanthomonas citri ssp. citri (Xac). The common symptoms are defoliation, twig die-back and premature fruit drop. The infection process of this bacterium is not totally elucidated although it has been reported that peptidases are involved in the infection and virulence process. The genome sequencing of the bacterium Xanthomonas citri ssp. citri enabled the detection of a gene that encodes an enzyme cysteine peptidase like, possibly involved in infection. Aiming to characterize this enzyme and determine its possible involvement in the infection process were performed: expression in recombinant Pichia pastoris, purification of protein and enzyme studies for characterization were done. The kinetic characterization of recombinant enzyme was performed through the hydrolysis of synthetic substrates Z-Leu-Arg-MCA and Z-Phe-Arg-MCA (Z = Carbobenzoxy; MCA = 7-amido-4-methylcoumarin), as well as the use of substrates with intramolecular fluorescence suppression AbzKVRSSKQEDDnp, AbzKLRSSKQ-EDDnp and AbzKIRSSKQ-EDDnp (ABZ = Ortoaminobenzoic acid; EDDnp = Ethylene diamine [2,4-dinitrophenyl]). The best catalytic efficiency (Kcat/Km) of the enzyme was found using the substrate AbzKIRSSKQ-EDDnp that has a isoleucine residue at position P2, showing that the recombinant enzyme (HISCPXAC) prefer aliphatic amino acid residue in this position. Inhibitory experiments of enzyme activity were performed using different cysteine peptidase inhibitors including CaneCPI-1, CaneCPI-2, CaneCPI-3, CaneCPI-4 and E-64 (L- transepoxysuccinyl-leucylamido-[4- guanidino]butane), resulting in a constant of inhibition (Ki) of 84.64, 0.088, 0.10, 0.012 and 1.214 nM respectively. The polyclonal antibody anti- HISCPXAC was produced in mouse and Western blotting analysis revealed that the antibody was able to recognize the recombinant purified cysteine peptidase and also the native cysteine peptidase from Xanthomonas citri ssp. citri strain 306 cultivated in media inductor of pathogenicity. The same antibody did not recognize the protein in a Xac knockout strain for the cysteine peptidase gene. Our results suggest that the cysteine peptidase from Xanthomonas citri ssp. citri may be involved in the bacteria infection and/or virulence.
Algoritmo narfo para mineração de regras de associação generalizadas não redundantes baseada em uma ontologia difusa
(Universidade Federal de São Carlos, 2009-04-29)
Miani, Rafael Garcia Leonel
;
Santos, Marilde Terezinha Prado
;
https://lattes.cnpq.br/9826026025118073
;
https://lattes.cnpq.br/9487235096598355
Traditional approaches for mining generalized association rules are based only on database contents, and focus on exact matches among items. However, in many applications, the use of some background knowledge, as ontologies, can enhance the discovery process and generate semantically richer rules. In this way, this paper proposes the NARFO algorithm, a new algorithm for mining non-redundant and generalized association rules based on fuzzy ontologies. Fuzzy ontology is used as background knowledge, to support the discovery process and the generation of rules. One contribution of this work is the generalization of nonfrequent itemsets that helps to extract meaningful knowledge. NARFO algorithm also contributes at post-processing stage with its generalization and redundancy treatment.
Estudo dos efeitos biológicos de uma desintegrina recombinante do veneno da serpente Bothrops alternatus, DisBa-01, sobre a musculatura da parede abdominal após indução de hérnia incisional em ratos
(Universidade Federal de São Carlos, 2009-04-29)
Oliveira, Claudio Ricardo de
;
Araújo, Heloísa Sobreiro Selistre de
;
https://lattes.cnpq.br/4065824911933203
;
https://lattes.cnpq.br/5091705745613726
INTRODUCTION: The incisional hernia (IH) is one of the most frequent complications after laparotomy, causing high rates of re-operations. Advances in suture material, in the guidelines for incisions, in the techniques of closure and use of dentures (screens), have failed in the elimination of this surgical complication. In this context, advances in the understanding of biological and structural changes of collagen may offer a new strategy in the treatment of IH, since the matrix disorders that induces the formation of primary hernia probably also affect the wound healing in laparotomies. OBJECTIVE: To investigate the effect of a recombinant desintegrin (DisBa-01) on the abdominal muscle healing process with incisional hernia (IH) regarding the fibroblasts and vessels proliferation and matrix metalopeptidase activity as well. The role of α2 and anti- αvβ3 integrins were investigated by antibody competition. METHODS: IH was induced by sectioning of the midline in 20 Wistar rats, divided into two groups: control that received the topical application of phosphate buffered saline (PBS) and DisBa-01 protein (0.5mg/Kg) dissolved in PBS. Another 10 rats were divided in two groups: one received anti-αVβ3 antibodies and the second anti-α2 subunit antibodies. At the end of 14 days, the animals were sacrificed and muscle fragments were stored in liquid nitrogen for determination of MMPs by zymography. The remaining fragments of the control and DisBa-01 groups were fixed in 10% formol in PBS (v/v). Blood vessels density, mononuclear cells, fibroblasts and collagen fibers were studied by optical microscopy in blue reticuline and Masson-trichrome. Data was analyzed by parametric tests were applied with critical level of 5%. RESULTS: All animals after surgical induction developed IH. However, after 14 days the persistence of the opened hernial ring in the controls but not in the DisBa-01 group. This protein induced the formation of a membrane that occluded the entire hernial ring. A significant increase in the number of mononuclear cells, fibroblasts, new vessels and MMP-2 activity was observed in DisBa-01 group (p<0.05). DisBa-01 group significant increased the collagen content. Anti-αVβ3 integrin antibodies produced similar results but not anti-α2 integrin blocking antibodies. CONCLUSION: These results indicate that DisBa-01 promoted vascular and fibroblastic cell proliferation and monocytic, and matrix remodeling. In addition, these results strongly indicate that αVβ3 integrin has an important role in the control of would healing and the blocking of this integrin may be an interesting therapeutical strategy in tissue repair conditions, including IH.
Aplicação de nanopartículas em filmes utilizados em embalagens para alimentos
(Universidade Federal de São Carlos, 2009-04-30)
Aouada, Márcia Regina de Moura
;
Mattoso, Luiz Henrique Capparelli
;
https://lattes.cnpq.br/5839043594908917
;
https://lattes.cnpq.br/2870655742911951
Dispersions composed of chitosan (CS) were obtained by the template polymerization (CS-PMAA) and ionotropic gelatinization (CS-TPP). The nanoparticles were analyzed by FT-IR analysis, particle size, zeta potential, electron microscopy (transmission and scanning), and TGA analysis. The nanoparticles obtained presented a very homogeneous morphology showing a quite uniform particles size distribution and a rather spherical shape. The particle size and zeta potential were dependent on the chitosan concentration used during the preparation method and pH. Different particles were incorporated in the hydroxypropyl methylcellulose (HPMC) films in order to improve their properties. For this, a 3% HPMC (in w:v %) solution was prepared and added in this nanoparticles solutions of both syntheses. The films were characterized by mechanical (tensile, elongation and elastic modulus), barrier [water vapor (WVP) and oxygen], thickness, morphological, and thermal properties. The WVP of the control HPMC film was 0.79 g mm/kPa h m2. The WVP decreased significantly when CS-PMAA nanoparticles were incorporated to HPMC films. For example, WVP decreased to 0.64; 0.59 and 0.47 g mm/kPa h m2 for films containing 59, 82 and 110 nm, respectively. For CS-TPP nanoparticles the WVP decreased to 0.58; 0.45 and 0.33 g mm/kPa h m2 for films containing nanoparticles with 220, 110 and 85 nm, respectively. The same way, the mechanical properties of the HPMC films were strongly improved when CS nanoparticles were incorporated. In this way, HPMC films containing nanoparticles could be considered as promising materials for application in packaging for preservation of foods.