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Estudo do potencial de regeneração de uma floresta estacional semidecídua, São Paulo, Brasil
(Universidade Federal de São Carlos, 2010-06-02) Laurito, Sabrina Ferreira; Soares, João Juares; https://lattes.cnpq.br/6093800182799882; https://lattes.cnpq.br/3949776696807179
Análise de expressão e splicing alternativo do gene Mdh-1 de Apis mellifera L. (Hymenoptera: Apidae)
(Universidade Federal de São Carlos, 2010-06-02) Nagamati Junior, Keize; Del Lama, Marco Antonio; https://lattes.cnpq.br/7198095288825585; https://lattes.cnpq.br/7577806509170147
Mdh-1 enzyme locus coding for cytoplasmic malate dehydrogenase has three common alleles Mdh-1100 (F), Mdh-180 (M) e Mdh-165 (S) and it has been extensively used as racial marker in populational studies of Apis mellifera. Additional isoforms are detected by electrophoretic analysis during ontogenetic development of A. mellifera, indicating differential expression of this locus, and a large set of evidences suggests that Mdh-1 alleles are under temperature-mediated selection. In this work, we proposed to study molecular aspects of this locus aiming to understand the nature of observed polymorphism and possible mechanisms leading to the formation of additional isoforms. Our results suggest Mdh-1100 as the ancestral allele that gave origin to alleles Mdh-180 and Mdh-165, and electrophoretic mobility differences of allelic products may be attributed to the substitution of a single aminoacid in each variant. Regarding genic expression during development, we re able to determine that both loci Mdh-1 and Mdh-2, coding for mitochondrial malate dehydrogenase, have high expression in larval and late pupal stages, and present a significant decrease in expression during transitional stages between larva and pupa. We also determined that additional isoforms are characteristic of pupae fat body and hemolymph, not being detected in other tissues, appearing at late larval stage and starting to disappear with beginning of pupal pigmentation. We were not able to identify the causes promoting the formation of the new isoforms, but our results suggest that this phenomenon is not due to alternative splicing or expression of a stage- and tissue-specific gene.
Localização de terminais intermodais na rede de escoamento da soja em grão brasileira destinada à exportação
(Universidade Federal de São Carlos, 2010-06-02) Almeida, Marina Soares; Morabito Neto, Reinaldo; https://lattes.cnpq.br/5992462757071905
Soybean is one of the most important agricultural products grown in Brazil. Comparing to the total volume of grain produced, soybean corresponds to approximately 44% and the midwest region is the largest soybean producer in Brazil. Because of that, the soybean that goes for export, on average, have to go over large distances to reach the maritime ports from where it is exported. Transport costs represent approximately 25% of the total cost what influences directly on the product price in international market. Loading transportation over long distances, such as the Brazilian soybean flows, the intermodal transportation is an alternative to make the soybean flows for export more economical. The efficient loading transportation using more than one modal depends not only on the availability of the transport itself but also on support infrastructure, such as intermodal terminals. In order to support decisions regarding the design and operation of the network flow of soybean for export, this work proposes using a mathematical programming model based on the Theory of Facilities Location to locate intermodal terminals in the network, taking into account not only the physical network, but also flows of soybeans that are moved by its vertices and arcs. We made several computational preliminary tests to verify the adequacy of the model to the reality we want to represent. Given the adequacy of the model, new experiments were done on a network that represents more realistically the present situation of the soybean flows in Brazil.