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Estudo das propriedades estruturais através de espectroscopia Raman de amostras de grafite de poucas camadas (GPC) obtidas por clivagem mecânica
(Universidade Federal de São Carlos, 2011-08-25) Burgos Gavelán, Ricky Nelson; Moreira, Fernando Manuel Araújo; https://lattes.cnpq.br/1809254923092721; https://lattes.cnpq.br/4542523190192267
In this work, We report studies on samples of graphite few layers obtained from micromechanical techniques of cleaving Manual of highly oriented pyrolytic graphite (HOPG). In order to checking the number of layers in each sample, each was physically characterized by spectroscopic measurements using the Raman technique. We studied the ratio of the intensities of the first-order band permitted G, and the second-order band resonance G′(2D) (IG=I2D), from Raman spectroscopy, provides useful information about stacking of graphite layers to verify the presence of few layers in the samples. The results of this study show that by Raman spectroscopy is possible to obtain information from the structural composition of the material and allows knowing the stacking of graphite layers in the samples. Since was used HOPG, there is no information of the size of crystallite represented by disorder induced D band , since this material has long range crystalline symmetry where the crystal size is much larger than the spot of laser used for the measurements.
Expansão in vitro de células-tronco mesenquimais cultivadas em biorreator de fibra oca
(Universidade Federal de São Carlos, 2011-08-25) Mizukami, Amanda; Suazo, Cláudio Alberto Torres; https://lattes.cnpq.br/9591447226240450; https://lattes.cnpq.br/1505095854164456
The mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into various types of tissue, a characteristic that makes them interesting for applications in cell therapy. Moreover, cells are anchorage-dependent (ability to adhere to surfaces), easy isolation and rapid expansion in vitro. The traditional cultures of anchorage-dependent animal cells, usually grown in monolayers, resulting in low crop yield and cell recovery, preventing the use of MSCs in therapeutic applications. The need for alternative farming techniques for expansion of MSCs in a large scale have led researchers to the use of "bioreactor technology," which has been seconded from the bioreactor, spinner flask with microcarriers and hollow fiber (hollow fiber). The objective of this work was to develop a method of cultivation known as mesenchymal stem cell line hMSC-TERT in spinner flasks with microcarriers for subsequent inoculation into hollow-fiber bioreactor, aiming at the efficient expansion and the subsequent recovery of MSCs so that preserve their differentiation potential in order to be used in cell therapy. In cultured, we used the microcarrier Pronectin ® F 100 mL spinner flasks with culture medium α-MEM with 15% fetal calf serum, kept in a CO2 incubator at 37 ° C and pH between 7.2 and 7.4. In cultures in spinner flasks were adopted strategies such as supplementation of the culture medium, exchanges of culture medium during cultivation, addition of reagents to reduce clusters of microcarriers, to increase productivity for the subsequent cell inoculation in hollow fiber bioreactor. The use of these strategies has increased productivity cell, achieving a best result of cell multiplication factor (MCF) of 5.79. However, obtaining higher FMC was drastically limited by the formation of clusters of microcarriers in a gel matrix.The cell recovery was low maintenance and analysis of the antigenic phenotype by flow cytometry confirmed the conservation of phenotypic characteristics of hMSC-TERT. The hollow fiber bioreactor is an alternative to the clusters of microcarriers, it allows oxygenation and adequate supply of nutrients. Thus, in experiments carried out in hollow-fiber bioreactor used the α-MEM supplemented with 15% FBS (v / v), 2.0 g / L glucose, 2.50 mM glutamine, 2.60 mM arginine, 0.07% antifoam PPG. The cells were inoculated after 48 hours of cultivation in spinner flasks in a gel composed of collagen, hyaluronic acid, agar (1.5%) in the proportion 0.75: 0.037: 0.21 at pH 7.2 to 7.4 . Thus, the resulting gel was mixed with culture medium in α-MEM 1:3 ratio, to provide better grip on the fibers. The total expansion factor, ie the one calculated from the inoculation of the spinner to the end of cultivation in hollow fiber bioreactor was 11, 2, which can be considered the greatest value achieved in this work. It is clear that this expansion factor can still be easily overcome by adopting strategies to grow more frequent replacement of culture medium. This hypothesis is strengthened by the proven fact that the microcarriers are not yet saturated with cells at the end of the experiment.
Mecanismo para garantia de privacidade em redes sociais online
(Universidade Federal de São Carlos, 2011-08-25) Botelho, Rodrigo Pereira; Zorzo, Sérgio Donizetti; https://lattes.cnpq.br/2523715806470871; https://lattes.cnpq.br/3020853775955526
The use of online social networks for sharing personal data is becoming increasingly common. To join an online social network, users must agree to the online social networks policies and this generally implies agreeing that all posted data can be used for various purposes, for instance, to improve the service offered by the social network operator. Online social networks generally provide tools to allow users to restrict access to their shared data. However, these access restrictions applies only to social network users, enabling third parties and the social network itself access and use to these data. A data encryption based approach only is insufficient to ensure privacy and preserve the ability of the user to obtain personalized services. This paper presents a mechanism based on a well-known message format coupled with the use of methods for symmetric and asymmetric encryption to extend the privacy in online social networks, ensuring that certain user data types remain private to other network users, third parties and own social network, yet allowing the offering of personalized services. Additionally, a case study using the mechanism in a real online social network and an analysis of the mechanism are presented.
Correlação entre acidentes de trânsito, uso e ocupação do solo, polos geradores de viagens e população na cidade de Uberlândia-MG
(Universidade Federal de São Carlos, 2011-08-25) Alves, Priscilla; Raia Junior, Archimedes Azevedo; https://lattes.cnpq.br/6413793013018019; https://lattes.cnpq.br/7816834312341689
A alegoria histórica em Manoel de Oliveira: um filme falado
(Universidade Federal de São Carlos, 2011-08-25) Santos, Wiliam Pianco dos; Paiva, Samuel José Holanda de; https://lattes.cnpq.br/7222933274927263; https://lattes.cnpq.br/0171958971463706
This research consists of a discourse analysis of Um filme falado (2003), from the Portuguese film director Manoel de Oliveira. The idea is to investigate the meanings implied in the historical allegory built by the director in that film, questioning the notions of nation and globalization. In this sense, the theoretical references are conducted to the implications of the allegory in cinematographic discourse, as well as the interdisciplinary field located between the Cinema and History. For such end, thematic and formal aspects will be dealt based on the film analysis of the work in question.