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Interações fitoplâncton-bactéria: associações específicas e possíveis implicações ecológicas
(Universidade Federal de São Carlos, 2013-07-03) Bagatini, Inessa Lacativa; Vieira, Armando Augusto Henriques; https://lattes.cnpq.br/4428377384377272; https://lattes.cnpq.br/3904631503604006
The occurrence of nuisance phytoplankton blooms is a worldwide problem that affects water quality and other aquatic biota. Bacterial (non-cyanobacterial) communities can influence and interfere with the formation, maintenance and termination of such blooms via many biotic interactions. The bacterial communities associated with 3 bloom forming phytoplankton species, the Cyanobacteria Microcystis aeruginosa and Cylindrospermopsis raciborskii and the diatom Aulacoseira granulata, were assessed by the 454-Pyrosequencing of 16S rRNA gene amplicons (V3-V5 regions). The aims of this work were to verify if different bacterial communities would develop with different host species, or in different fractions (attached and free-living bacteria) and different growth phases (physiological state) of each phytoplankton species. Still using pyrosequencing data, the strength of phytoplankton-bacteria association was analyzed for M. aeruginosa and A. granulata by culturing the phytoplankton species with different bacterial inocula (and growth medium for the cyanobacteria). Furthermore, the effects of bacterial strains isolated from attached community of A. granulata and M. aeruginosa cultures were tested on the growth of the phytoplankton species. Significantly different bacterial communities developed: i) in response to physiological state and between fractions of the same phytoplankton culture, and ii) in response to the different host phytoplankton species, with major differences in the proportion of the OTUs between phytoplankton cultures rather than in the absence or presence of specific bacterial taxa. Differences between experiments significantly changed the BCC that developed associated with the same host species. However, some bacterial OTUs were found associated with the same phytoplankton species in different experiments, suggesting a tight association with the host species. Most of the strains either isolated from M. aeruginosa or from A. granulata affected negatively the growth of the diatom. However, the isolated strains had no effect on the cyanobacterial growth, suggesting the importance of bacterial community to the dominance of M. aeruginosa on Barra Bonita Reservoir, at least concerning to the competition with A. granulata.
A construção do atendimento em um núcleo de medidas socioeducativas em meio aberto
(Universidade Federal de São Carlos, 2013-07-03) Munhoz, Sara Regina; Villela, Jorge Luiz Mattar; https://lattes.cnpq.br/8301325600502054; https://lattes.cnpq.br/7134597984469299
This research aims to discuss the data from my ethnography of an assistance nucleus (MSE / MA) to adolescents who practices infractions in the East Zone of São Paulo. The ethnography was conducted with a team of professionals that assists these boys, and emphasizes the perceptions that these professionals have of the construction of assistance, and the relationships they establish with the boys, with the judiciary and other government agencies. I describe the ways the team handles, on the one hand, with the rigidity of established goals by judges and described in the legislation and, on the other hand, with requirement also legal that assistances are individualized. To deal with these two logics, and present to the judges the knowledge that can only be built from the nucleus, technicians use the writing and interpretation of documents as their main weapons. It is a routine exercise of building arguments that justify the paths taken in each assistance. I also describe the frequent moments that the technicians can not extend their perceptions to the judiciary, causing divergent views on the progress of cases. I argument, therefore, that the relationship between the team and the judges is always based on the uncertainty and the definition of assistance needs to be negotiated in each case. This uncertainty manifests itself by the coexistence, in the nucleus, of different knowledge and different forms of governance of the boys.