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Estudo comparativo de indicadores de saúde bucal em modelos assistenciais da Atenção Básica de São Carlos/SP a partir do SIA-SUS
(Universidade Federal de São Carlos, 2013-09-17) Magri, Laís Valencise; Silva, Geovani Gurgel Aciole da; https://lattes.cnpq.br/6146469366214279; https://lattes.cnpq.br/0408259619288134
The planning and evaluation of oral health practices are essential in the context of primary care. In this sense, the oral health indicators are important instruments for quantitative analysis, which can be used with these purposes. The aim of this study was to compare four oral health indicators [Income Indicator (II) Attrition (AI), Modified Prevention Emphasis (MPEI), Indicator Value Restoration / Extraction (IVRE), and the Relationship First Consultation/Urgency (RFCU)] in the Family Health Units (FHU) and Basic Health Units (BHU) in São Carlos/SP, in the period 2008-2011. Study participants were 15 FHU and 11 BHU. The data of the dental procedures performed were extracted from the Outpatient Information System (SIA-SUS) and the indicators were calculated according Narvai (1996). Statistical analysis was performed by Mann- Whitney test, adopting a significance level of 5%. The average (standard deviation) of II, AI, MPEI, IVRE and RFCU during the study period were, respectively, 20 (10.9), 7.5 (17.8), 0.06 (0.24); 5.2 (1.4), 0.7 (0.6) to BHU and 18.2 (12.2), 13.1 (20.1), 0.12 (0.33); 4.7 (2.1), 0.3 (0.5) for the FHU. Significant differences (p <0.05) were observed for IVRE and RFCU. It can be concluded that although BHU have shown a better performance than the FHU in São Carlos, in the period studied, the results also indicated a strengthening process of the Family Health Strategy. MPEI, AI and RFCU showed unsatisfactory results, pointing to the difficulty in implementing prevention and health promotion, difficulty in reaching the target audience and dental care focused on emergency and curative procedures. IVRE and II showed the best results, indicating high productivity in terms of dental care and a model focused on conservative practices.
A democracia no pensamento político dos comunistas brasileiros (1979-1983)
(Universidade Federal de São Carlos, 2013-09-17) Ramos, Carlos Alexandre; Cepêda, Vera Alves; https://lattes.cnpq.br/4917331423373631; https://lattes.cnpq.br/0352823597245001
This research investigates the "democratic issue" in the political thought of the Brazilian communists. Examines how it was conceived and treated inside and around the Brazilian Communist Party (PCB) over his career, with greater interest in the period between 1979 and 1983. Seeks to demonstrate the existence of a process of emergence of "democratic issue" with its shift to the centrality of this thought. This process has been accompanied by a redefinition of the concept of democracy as the basis of their political practice. Besides extensive bibliography, sources surveyed are classified basically into three types: a) party documents, such as resolutions, reports and notes, b) publications linked to the party, such as newspapers and magazines c) theoretical production of "organic intellectuals "Party. The first chapter recalls the genesis of this thinking, identifying it in the 1940s, The second chapter discusses the formulation of the "new politics" of the PCB from 1956 with the process of "de- Stalinization" which culminates in the document known as the Declaration of March 1958. The third chapter seeks to demonstrate the "change" that suffers this thought in the "decisive moment" between 1979 and 1980, analyzing the influence of theoretical and political authors as Lukacs and Gramsci and movement Eurocommunist and also the effects of the split between Luiz Carlos Prestes and PCBs. The fourth chapter deals with the expression of this "new" thinking between 1980 and 1983, especially in the way he was treated in the newspaper Voice of Unity. The results indicate some "traces" fundamental outlined this "democratic thought." As an expression of "continuity" in relation to the genesis and development identifies the continuing design politicist understood as choice of institutional policy, the defense reiterated the broad front multiclassist and multiparty, processes Constituents and participation in elections. As expressions of "breaks" (or attempted breaches), identifies the distance of dual loyalty towards the "matrix Soviet Marxism" and how they sought to assimilate and adapt to changes in the country's political agenda, which was characterized in the last decades by a significant change in the correlation strength on the one hand, between economics and politics, and on the other, between state and society, especially the appreciation of "civil society".
Produção de penicilina G acilase por organismos geneticamente modificados
(Universidade Federal de São Carlos, 2013-09-17) Escallón, Ana María Vélez; Giordano, Raquel de Lima Camargo; https://lattes.cnpq.br/0003899410308014
Penicillin G acilase (PGA) is a key enzyme in the industrial production of β-lactamic antibiotics. In this work, it was studied the production of PGA from Escherichia coli by recombinant E. coli and from Bacillus megaterium by recombinant B. megaterium. In E. coli, the enzyme accumulates in the periplasmic cell space, B. megaterium secretes PGA, what may reduce downstream costs. With E.coli, the study has begun at the expression step. Using a donated recombinant microorganism, it was studied, in shake flasks cultures, the influence of temperature (ranging from 18 to 28°C) during induction phase in the PGA production. High level expression of PGA E. coli was detected at 20°C, which was 4-fold superior than the volumetric enzymatic activity reached at 28°C. Fed-batch cultures were conducted with glycerol as carbon source, using both defined and complex media as well as IPTG and lactose as inducers. Final biomass concentrations of 100 gDCW/L and 120 gDCW/L and enzymatic activity 210000 and 80000 IU/L were achieved, for complex and defined media, respectively. The study with B. megaterium was initiated isolating the pac gene, encoding for PGA from B. megaterium ATCC14945, which was after cloned into the pLipAhp expression plasmid, together with the promoter gene and a gene codifying antibiotic resistance. This construct (pga-pLipAhp) was used to transform three different B. megaterium strain protoplasts, aiming studies to determine the best expression host in terms of PGA production. The tested strains, B. megaterium PV361, QM B1551 and ATCC 14945, have respectively shown about 95%, 95% and 10% plasmidial stability, after eight consecutive growths. The best PGA production was detected in PV361. Study of thermal and pH stability with the purified enzyme showed that PGA has a half-life of 5 min at 60° C, 20 min at 50° C, keeping 100% of activity for 1h at pH 10.0 and up to 8 h at pH 5.0. The values of temperature and pH for maximum PGA activity are 37° C and 8.0, respectively. The enzyme was stable at least for 5 hours in these conditions, with Michaelis-Menten estimated as Km=8.8 μM. A systematic study was developed to search the best condition to obtain the highest level of B.megaterium PGA production by recombinant B.megaterium. Media compositions for biomass and PGA production were evaluated using a genetic algorithm. The screening was carried out in 96 microtiter deep well plates, starting as a minimal medium and studying the concentration of 12 defined components. In 7 generations, 240 different kinds of media were tested for production and secretion and a 10-fold increase in PGA production and 5-fold increase in biomass compared to the previously used minimal medium could be achieved. It was scaled-up to shaker flasks obtaining 3-fold in PGA production and 1.8-fold in biomass. Later, it was scaled-up to bioreactors obtaining 3-fold in biomass and 8-fold in PGA production. Using glycerol as carbon source, it was tested different complex amino acids sources, using flask cultures. The best medium was scale-up in a 5 L bioreactor, in a batch culture with pulses. The highest production of PGA of recombinant B. megaterium achieved was 105600 IU/L and 36 gDCW/L.