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FrAMC-i: um framework de apoio à produção de conteúdo multimídia complementar interativo
(Universidade Federal de São Carlos, 2014-08-27)
Rodrigues, Kamila Rios da Hora
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Penteado, Rosângela Aparecida Dellosso
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https://lattes.cnpq.br/4061946080136286
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https://lattes.cnpq.br/9035684997439778
The television is the medium of information and entertainment most utilized by Brazilians. Despite its great reach, this vehicle, which should be an instrument to promote social inclusion, often causes the opposite effect. The manner some television programs are produced, in particular movies and series, combined with the lack of prior knowledge about certain subjects and contexts, trigger some viewers not to appreciate or enjoy enough of the programming, which may lead to a possible dissatisfaction in the audience. Cultural and educational matters may also accentuate the viewer's discontentment during a television session. New technologies such as the Interactive Digital TV (IDTV) allow the enrichment of television media with additional contents that may provide greater fruition of the narratives. This PhD project benefits from the flexibility and interactivity offered by the IDTV to propose a solution of accessibility based on the offer of additional specific contents, here denominated interactive Additional Multimedia Content (AMC-i). The AMC-i are extra information associated with the television media that may be offered to the main program considering different aspects, such as whether the offer: is synchronous or asynchronous, on demand or compulsory, with or without pausing the original program, by combining one or more media formats, among others. Through case studies, groups of spectators with distinct profiles, in different scenarios and interacting with varying kinds of media, were observed and had their interaction difficulties and experiences evaluated. This thesis proposes and presents the FrAMC-i, a framework consisting of a set of guidelines that support the production of AMC-i and emerged from the experience acquired during the observation of spectators in the studies. In addition to the guidelines, the FrAMC-i also provides tools for the enrichment of the media with AMC-i and a mobile application that enables the interaction with such contents by usage of a second screen. The framework was submitted to assessment by sixteen graduate students in Computer Science in the role of designers. These evaluators used the FrAMC-i artifacts to enrich media, given a provided fictional scenario. The results suggest a positive evaluation considering the utility, adherence to the proposed design solutions and usability of the artifacts supplied by the framework for the different stages of the production of the AMC-i.
Diretrizes, projetos, sala de aula: passos e descompassos da educação jurídica nos cursos de direito do estado de São Paulo
(Universidade Federal de São Carlos, 2014-08-27)
Mascaro, Arnaldo Braga
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Tagliavini, João Virgílio
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https://lattes.cnpq.br/6475591370235205
This dissertation aims to demonstrate the steps and missteps in legal education in law courses in the state of São Paulo based on the analysis of Curriculum Guidelines and Political Pedagogical Projects (PPPs) and of their effectiveness in courses, through sampling. At this study s core is the concept that, at the level of discourse, the guidelines and PPPs are well developed and have content and methodology in accordance with the most recent findings in educational science. However, results have demonstrated the divergence between theory and practice. Taking into account that the state of São Paulo offers 220 of the nearly 1300 law degree programs available in Brazil, an analysis is performed of their necessity when less than half of college-aged Brazilians are enrolled in degree programs, a figure promised by the National Education Plan and the debate over inclusion with quality vs. exclusion. This analysis is done through a reading of the PPPs available at the Legal Education Commission of the Brazilian Lawyer s Order in São Paulo, which enabled an evaluation of the courses from 2010 to 2012, as well as interviews with members of the Commission. The present dissertation also draws from a thorough review of all law degree programs in the state of São Paulo, and their data was processed so as to be able to be accessed, altered and completed, thereby assisting researchers and the research group Education and Law in Contemporary Brazilian Society UFSCar, of which I am a member.
Descoberta dinâmica, sensível ao contexto, de serviços web
(Universidade Federal de São Carlos, 2014-08-28)
Silva, Victor Gomes da
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Prado, Antonio Francisco do
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https://lattes.cnpq.br/2668568143800755
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https://lattes.cnpq.br/1888388000918745
Currently the Internet is one of the biggest media in the world, turning and accelerating forms of publishing and content consumption. At the same time, users have become more demanding for the content and the consumption of large amount of information that the Web provides. Since its inception, the Internet has gone through some phases, showing that the changes take place according to the requirements of users, such as the evolution of the Web to Web 2.0 which is marked by the interaction, collaboration and communication among users, who can publish and share their own content on the Internet. This reality has led to the need to build applications that support the large amount of information to users and different devices, arranged in multiple contexts of use, increasing the demand for software in Ubiquitous Computing, in which access to applications occurs anywhere, anytime and from different devices. The construction and maintenance of specific versions of applications for each context of use have become difficult due to the diversity of devices, users, access networks and other factors to be considered. Moreover, it is necessary for applications to be developed so that the compatibility of the contextual features of the environment in which they operate is maintained and does not impair the interaction of users. Motivated to overcome these challenges this work presents an architecture for Dynamic Discovery of Context-Sensitive Web Services that supports the creation of ubiquitous applications adaptive to different contexts. Applications driven by this architecture meet varying access networks in different contexts. The proposed architecture is based on the concepts of Service Oriented Architecture. The proposed architecture was evaluated with a case study and experimentation with users. The computational support that employs the proposed architecture has the advantage of discovering services at run time and can vary according to the context of the access network.
Controle preditivo não linear para sistemas de parâmetros distribuídos
(Universidade Federal de São Carlos, 2014-08-28)
Rodríguez, Diana Esperanza Sandoval
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Kwong, Wu Hong
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https://lattes.cnpq.br/0682354960749546
In general, the chemical processes can be represented using mathematical models, in the case of lumped systems include ordinary differential equations, or, partial differential equations, when distributed parameter systems using methods is necessary in both cases numerical resolution in these models, with the purpose to simulate, analyze and control the process. The implementation of control systems in chemical processes, brings with it many advantages, among these, the improvement and stability in production rates, ensuring product quality and the possibility of a safe operation of the process. Thus, in the last 30 years, different control methodologies were developed, one of the most used techniques, the Model Predictive Control Based on. Its success is due to the fact that this type of control accepts constraints on input variables and process output, determining the future of this movement, while optimizing an objective function can lead to the output of the process until the desired set point. The objective of this project is to implement the Model Predictive Controller with Nonlinear (CPNL ) for a reactor pulp bleaching by the use of chlorine dioxide, whose mathematical model is comprised of partial differential equations, thus being a model parameter distributed. Implementation of the controller, the plant is discretized by the Finite Difference Method and the process model is solved with the technique of Orthogonal Collocation. The integration of the resulting ordinary differential equations systems is performed by the method of Runge-Kutta. The Predictive Controller was compared with a Controller Proportional-Integral (PI). Studies have shown that CPNL has better performance, with faster response and values of the Integral Absolute Error (IAE) and Integral Square Error (ISE) smaller than those calculated for the PI controller.
Estudos estruturais de duas 3 (Fenoximetil)-4-fenilbut-3-en-onas e docking no fator letal (LF) do bacillus anthracis (Antraz)
(Universidade Federal de São Carlos, 2014-08-28)
Nucci Junior, Paulo Roberto
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Moreira, Fernando Manuel Araújo
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https://lattes.cnpq.br/1809254923092721
In this work the crystal structures of two 3 (phenoxymethyl)-4-phenylbut-3-en-ones were determined and the resulting 3D strutures were used as input for docking studies in the lethal factor (LF) of bacillus anthracis. The results were then compared with those of a known inhibitor.(3E)-3-(4-nitrophenoxymethyl)-4- phenylbut-3-en-2-one (1): the conformation about the C═C double bond [1.348 (2) Å] is E with the ketone group almost co-planar [C C C C torsion angle = 7.2 (2)°] but the phenyl group twisted away [C C C C =160.93 (17)°]. The terminal aromatic rings are almost perpendicular to each other [dihedral angle = 81.61 (9)°] giving themolecule an overall U-shape. The crystal packing feature benzene-C H O (aldehyde) contacts that lead to supramolecular helical chains along the b axis. These are connected by π π interactions between benzene and phenyl rings [inter-centroid distance = 3.6648 (14) Å] resulting in the formation of a supramolecular layer in the bc plane.(3E)-3-(2,4-dinitrophenoxymethyl)-4-phenylbut- 3-en-2-one (2): the conformation about the C=C double bond [1.345 (2) Å] is E, with the ketonemoiety almost coplanar [C C C C torsion angle = 9.5(2) °] along with the phenyl ring [C C C C = 5.9 (2) °]. The aromatic rings are almost perpendicular to each other [dihedral angle = 86.66 (7) °]. The 4-nitro moiety is approximately coplanar with the benzene ring to which it is attached [O N C C = 4.2 (2) °], whereas the one in the ortho position is twisted [O N C C = 138.28 (13) °]. The molecules associate via C H O interactions, involving both O atoms from the 2-nitro group, to form a helical supramolecular chain along [010]. Nitro nitro N O interactions [2.8461 (19) Å] connect the chains into layers that stack along[001]. The docking results, using as a target the lethal factor (LF) of bacillus anthracis, show that both Compound 1 and 2 located themselves in the same cavity where the known inhibitor is located, and making most of the interactions this last one does with the amino acid residues that are important for the enzyme activity, so that it can be postulated that they can be also inhibitors. Moreover, Compound 1adopts a pose closer to that of the inhibitor whereas Compound 2 is rotated so that an important interaction is missed, this may indicate that this last one can be a less effective inhibitor than Compound 1.
Consultas por similaridade e mineração de regras de associação: maximizando o conhecimento extraído de séries temporais
(Universidade Federal de São Carlos, 2014-08-28)
Andrade, Claudinei Garcia de
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Ribeiro, Marcela Xavier
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https://lattes.cnpq.br/0300141044144026
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https://lattes.cnpq.br/5690286318736653
A time series analysis presents challenges. There is a difficulty to manipulate the data by requiring a large computational cost, or even, by the difficulty of finding subsequences that have the same characteristics. However, this analysis is important for understanding the evolution of various phenomena such as climate change, changes in financial markets among others. This project proposed the development of a method for performing similarity queries in time series that have better performance and accuracy than the state-of-art and a method of mining association rules in series using similarity. The experiments performed have applied the proposed methods in real data sets, bringing relevant knowledge, indicating that both methods are suitable for analysis by similarity of one-dimensional and multidimensional time series.
Trailer do filme Psicose, para além do marketing cinematográfico
(Universidade Federal de São Carlos, 2014-08-29)
Constantino, Maria Julia évora
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Monzani, Josette Maria Alves de Souza
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https://lattes.cnpq.br/0505156751073470
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https://lattes.cnpq.br/8654431191020019
The objective of this dissertation is to study the narrative of the trailer of one of the most influential films in cinema history: Alfred Hitchcock´s Psycho (1960). The trailer will, therefore, be analyzed not only as a tool of movie marketing, but mostly as a 'shortmovie' which has a kind of parallel narrative and assist in the feature film, with a beginning, middle and end, trying to understand how that is constituted and how it still speaks to the disclosure´s posters and the opening credits of the film. Both 'core' sequence storyboard´s (trailer and movie) will also be used in this reading that seeks to comprehend the process of signification in the orbit of Psycho.
Bergson e o eu dividido
(Universidade Federal de São Carlos, 2014-08-29)
Ribeiro, Eduardo Soares
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Marques, Silene Torres
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https://lattes.cnpq.br/4822141288006918
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https://lattes.cnpq.br/1452378874288074
This work is aimed at investigating the inner life in Bergson s first book, Time and Free Will An essay on the immediate data of consciousness (1889). We try to analyze especially the notions of duration and space in light of what we call the divided self , i.e., the division established by Bergson between the superficial self related to space, social life and language and the deap-seated self which concerns the pure duration of consciousness, the continuous multiplicity of interpenetration of psychological states and creating and unpredictability that constitutes each free act. At the end, and already in possession of the conclusions on the Essay, we will discuss the bergsonian intuitive method and its relationship with the divided self.
Análise de risco do aproveitamento da água de chuva para uso não potável em edificações
(Universidade Federal de São Carlos, 2014-08-29)
Bassanesi, Karine
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Barreto, Douglas
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https://lattes.cnpq.br/7369326190168139
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https://lattes.cnpq.br/4154058724586056
This master degree contains a literature review on the conservation of water, rainwater utilization, quality and water treatment, legislation and risk analysis. Was verified that rainwater utilization systems vary widely in type and would be important to be able to correlate the precipitated water, uses and models for each situation. And for this work was used the risk analysis focusing the preliminary hazard analysis. A bibliographic review carried out presented some guides and legislation that could complete quality control parameters for non-potable rainwater utilization, and determine the time to review these parameters to control the operation of the system. The study's preliminary analysis was used to study the greatest dangers in the use of rain-related water quality system, critical control points, the user risks, control measures and mitigation measures. For the case study was addressed the same preliminary hazard analysis for knowledge about the contamination of rainwater in relation to the control parameters and on their own knowledge of project development for rainwater utilization. Through the two analyzes a proposal of guidelines was performed for implantation of rainwater utilization system for the case study and there is a possibility of a real fitness for safe use.
Teores de nutrientes minerais e metais pesados em açúcar mascavo produzido por diferentes sistemas orgânicos e convencionais
(Universidade Federal de São Carlos, 2014-08-29)
Luchini, Paulo Dirceu
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Borges, Maria Teresa Mendes Ribeiro
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https://lattes.cnpq.br/1800048119205212
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https://lattes.cnpq.br/1639064301786387
The levels of Cu, Zn, Mn, Fe, Pb and Cd were determined in brown sugar from sugar cane produced by different crop systems in the organic and conventional systems. Pb e Cd were determined by atomic absorption spectrometry with electro thermal atomization in graphite furnace (GFAA), and the mineral metals Cu, Zn, Mn and Fe were determined by flame atomic absorption spectrometry (FAAS). Cu, Zn, Mn and Fe were detected but in levels below the limits recommended by the World Health Organization. The brown sugar from the conventional system without limestone but with chemical fertilizer showed the highest concentration of Cu, Zn, Mn and Fe, and that from the organic system with liming and organic compost showed the lowest concentrations. The levels of Pb found in samples from: conventional system with organic amendment and chemical fertilizer, and with liming and chemical fertilizer, and from organic system with liming and organic compost were higher than the tolerable limit for the Brazilian National Health Surveillance Agency for sugar. Cd was never detected. The results indicate the need of constant monitoring studies of the brown sugar produced in organic systems to assure its food safety.
Instituições participativas: audiências públicas para revisão do plano diretor no município de Araraquara
(Universidade Federal de São Carlos, 2014-08-29)
Lopes, Maira Gonçalves
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Lahuerta, Milton
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https://lattes.cnpq.br/9326718732298388
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https://lattes.cnpq.br/8027586789638304
On the traversed course through the literature about institutional participation, it is possible to point distinct moments to deal with. In this dissertation was recovered the discussion approached by Carlos Estevam Martins, that in the end of the eighties and begin of nineties, bring forward the participation theme into the theory of the State and highlighted the relationship of participation and representation in this context. Following, we brought to the debate inspired research by the Habermasian theory, that pointed to an enthusiasm with the new forms of institutional participation. Subsenquently, research showed that was a large disparity between empiric bases and normative expectations in the literature until then. The last and current moment, focuses its efforts to the effective question of participation. During this period, many participation institutions (PIs) was analyzed and evaluated, however, the research about Public Hearings was rare. Based in the literature balance, this dissertation proposed to analyze Public Hearings, in the municipality of Araraquara-SP( Brazil), that was made throughout the revision process of the Master Plan of this city in 2013. The research point that the hearings, because of yours advisory character, worked as debate environments, but not as a political instrument of propositions and decisions. In addition, this Public Hearings operate as a subordinate participation instrument of government authority, because is that one who is able to incorporate or reject submitted suggestions by the population in this environment.
Ouvindo vazios : os silêncios em A erva do rato e Cleópatra de Júlio Bressane
(Universidade Federal de São Carlos, 2014-08-29)
Terezani, João Henrique Tellaroli
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Miranda, Suzana Reck
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https://lattes.cnpq.br/8566012598222563
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https://lattes.cnpq.br/4659424010227734
This work aims to discuss the representation of silence in films. Our objects of study are the films Cleópatra and A erva do rato both by Julio Bressane, since the phenomenon of silence emerges as a recurring theme in the style of this director. The analyzes found in this work focus on the discussion of the phenomenon linked to its discursive, and its physical appearance, and also about its manifestations in artistic fields like music, poetry and cinema. To do so, we had as main references studies of Paul Théberge, Fernando Morais da Costa, Virginia Flores about the sound in cinema, John Cage, Susan Sontag, José Miguel and Jorge Antunes Wisnik on the representativeness of silence for the arts in general.
Participação da neurotransmissão orexinérgica nas respostas respiratórias à hipercarbia e hipóxia em sapos
(Universidade Federal de São Carlos, 2014-08-29)
Fonseca, Elisa Maioqui
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Batalhão, Luciane Helena Gargaglioni
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https://lattes.cnpq.br/5850453468994497
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https://lattes.cnpq.br/4029501980983015
The hypocretin or orexin A plays an important role in the modulation of respiratory control in mammals, but there are no data available for the role of Orexins in the peripheral and central chemoreception of non-mammalian vertebrates. Thus, the present study was designed to evaluate the location of orexinergic neurons in toads (Rhinella schneideri). In addition, we investigate if the orexinergic system of this species is important to hypoxic (5% O2 and N2 for balance) and hypercarbic (5% CO2, 21% O2 and N2 for balance) drive to breath. We assessed the role of the orexinergic system on respiratory responses by using intracerebroventricular injection of SB-334867 (orexin A receptor antagonist) during the light and the dark phase. Our results demonstrated that orexinergic neurons of Rhinella schneideri are located in the suprachiasmatic nucleus of diencephalon. Additionally, the injection of the orexin antagonist attenuated the ventilatory response to hypercarbia during the dark phase by acting on tidal volume and breathing frequency, while in the light phase, there was an attenuation in the ventilatory response to hypoxia by acting just in the tidal volume. We conclude that central orexin A contributes to hypercarbic and to hypoxic chemoreflex in toads Rhinella schneideri.
Estudos estruturais e funcionais de duas β-glicosidases de Trichoderma harzianum
(Universidade Federal de São Carlos, 2014-08-29)
Mutti, Hêmily Sanches
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Polikarpov, Igor
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https://lattes.cnpq.br/9669532724764871
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https://lattes.cnpq.br/2114370378373874
The depletion of fossil fuels, the growing energy demand and the great need to reduce carbon emissions gradually increased the demand for new sources of renewable and clean energy. Bioethanol is obtained from the fermentation of lignocellulosic biomass, for example sugar cane, and can be considered a viable alternative. For this biomass can be used it is necessary to degrade the constituent molecules of the cell wall to fermentable sugars through the enzymes called cellulases. Among them there are three classes with different functions: the cellobiohydrolases, endoglucanases and β-glucosidases. In order to contribute to the viability and deployment of ethanol production technologies, purification of two β- glucosidases of the fungus Trichoderma harzianum was performed, named in this work as ThBgl1 e ThBgl2, as well as their biophysical and biochemical characterization. Clones in study were obtained by a cloning platform, expression and purification of recombinant proteins in high performance and expressed in bacteria Escherichia coli. Through the functional characterization of proteins under study it was found that they had the optimum pH in the range of 5.5 and optimum temperature around 40ºC. Results of enzyme kinetics showed that ThBgl1 has higher preference (specificity) by cellobiose, while in ThBgl2 cellobiose is a substrate having low specificity. In ThBgl2 specificity is higher by fucosideos. Furthermore, it was observed the occurrence of transglycosylation catalyzed by β-glucosidases in study and the action of some inhibitors on its enzymatic activity. The three-dimensional analysis of these proteins revealed the presence of the barrel tim typical of family 1 of glycoside hydrolases.
Uso da modelagem molecular na solução de problemas em química
(Universidade Federal de São Carlos, 2014-08-29)
Madureira, Lucas Sousa
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Schpector, Júlio Zukerman
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https://lattes.cnpq.br/4252331837170383
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https://lattes.cnpq.br/7944224842300652
The studies presented herein show the use of molecular modelling coupled to spectroscopic data to tackle four different problems in organic chemistry. In the first study, the sulfonylation of the primary hydroxyl group of the compound (2R,3S,6S)-2-(hydroxymethyl)-6-(1-phenyl-1H-1,2,3-triazol-4-yl)-3,6-dihydro-2Hpyran- 3-ol was addressed. The results show that the occurrence of the reaction depends on the stability of the S Cl secondary interactions and of the C-H Cl hydrogen bonds along the reaction pathway. The second investigation was the cycloaddition between benzyl azide and (organotelanil) alkynes. This reaction has a high driving force towards the products and generates fast and selectively a triazole with a n-butyltelluride substituent attached at position 5, which is stabilized by CH...Te interactions. So this cycloaddition fits the requirements of Click Chemistry. In third place, the conformations and relative configurations of 7 and 9 membered rings were elucidated by evaluating steric strain. The 7 membered ring shows a twisted chair conformation (TC) with C1, C7 and C10 having relative configurations S, R and S. The 9 membered ring has a twisted chair-chair conformation (TCC) and relative configurations R, S of C7 and C10. The fourth study was the optimization of rotational conformations of 1- (arylsulfonyl)indole derivatives. The different optimized conformations have the same stability and low rotational energy barrier, so that in the solid state the number of independent molecules in the asymmetric unit essentially depends on the packing intermolecular interactions. Regarding the stability of sulfonamide bonds the importance of hyperconjugative effects were showed, and the origin of the rotational barrier in the S-N bond is explained by the difference in steric hindrance and electrostatic interactions in the transition and the fundamental states.
O conceito de subjetividade em A Ideia da Fenomenologia
(Universidade Federal de São Carlos, 2014-08-29)
Souto, Andressa Alves
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Ferraz Neto, Bento Prado de Almeida
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https://lattes.cnpq.br/0084436724293824
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https://lattes.cnpq.br/2748513205466368
The objective of this research is the study of the concept of subjectivity as such it develops in lessons on The Idea of Phenomenology (1907), by Edmund Husserl. Such concept in this work is linked to a critique of the traditional conception of representation, according to which the psychic immanence designate one intramental ontological region in which they find representations as substitutes for reference objects. To the extent that this criticism is made, the relationship between immanence and transcendence are redefined, providing a new phenomenological concept of subjectivity. This, in turn, is founded on the idea of authentic immanence, which includes not only sensations and acts (real [reell] immanence), but intentional objects in their modes of appearance (intentional immanence). This conception of subjectivity signals a change in relation to what had been advocated in the work initiating the Husserlian phenomenology - the Logical Investigations (1900-1). At this, subjectivity is presented as containing only the noetic character of knowledge - the real immanence -, while in the work studied here it also includes what subsequently one designate, in phenomenology, as noematic character of knowledge the immanence in intentional sense. The hypothesis of reading proposed here is that this conception of subjectivity is made possible by analysis of Husserl on time-consciousness, from 1905, in which it reveals that even within the "flow" of experiences, there is something identical, which beyond the scope of real immanence. This recognition would have led Husserl to the conception that the limitation of subjectivity to the sphere of real immanence is founded on a mistaken association between the latter and immediate evidence. Therefore, the route of Husserl's text in The Idea of Phenomenology will moves itself around the dissociation between these two notions. Draw will be, therefore, the path taken by Husserl in this work in order to highlight the notion of subjectivity that emerges in it.
Estudos moleculares, estruturais e funcionais da Cel12A de Gloeophyllum trabeum, uma endo-1,4-β-glucanase da família 12 de hidrolases de glicosídeos
(Universidade Federal de São Carlos, 2014-08-29)
Miotto, Lis Schwartz
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Polikarpov, Igor
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https://lattes.cnpq.br/9669532724764871
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https://lattes.cnpq.br/5902555075011962
The production of second-generation ethanol by enzymatic hydrolysis of biomass is considered a viable and promising alternative to face the global energy crisis and to decrease our dependence on fossil fuels. Therefore, it is necessary to degrade the constituent molecules of the plant cell wall such as lignin, cellulose and hemicellulose to fermentable sugars. However, the use of enzymes for this purpose is still expensive, leading to the increase on studies seeking to make them more feasible economically and technically. The present study aimed the molecular, structural and functional characterization of the endoglucanase Cel12A from the fungus Gloeophyllum trabeum by different techniques. Biochemical data revealed the substrate specificity for the enzyme and showed that β-glucan is the best substrate for its activity (239.2 ± 9.1 U mg-1). Optimal conditions for activity were pH 4.5 and temperature of 50 oC. Thermal stability assay indicated a half-life of 84.6 ± 3.6 hours at 50 oC. The kinetic parameters Km (3.2 ± 0.5 mg mL-1) and Vmax (0,40 ± 0,02 μmol min-1) were determined using β-glucan as substrate. Analysis of scanning electron microscopy of oat spelts and filter paper samples submitted to the hydrolysis by GtCel12A evidenced the degradation effects of these substrates compared to control samples. Moreover, the low-resolution envelope and the crystallographic structure for GtCel12A were determined. The structure revealed a β-sandwich fold with two β-sheets (A and B) and three α-helices, while sheet A showed five strands and sheet B nine strands. The comparative analysis of the amino acid sequence and homologous structures prompted us to identify the catalytic residues, Glu142 and Glu227 in the active site of the enzyme. These results are important for understanding and elucidating the enzyme molecular mechanism of action and other glycoside hydrolase family 12 endoglucanases.
Desenvolvimento e caracterização de liga Fe-Mn-Si-Cr-Ni com efeito de memória de forma
(Universidade Federal de São Carlos, 2014-08-29)
Simon, Rafael Wagner
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Giordani, Enrico José
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https://lattes.cnpq.br/7477710392592430
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Kuri, Sebastião Elias
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https://lattes.cnpq.br/6842072916153866
The shape memory effect represents a physical property of materials with great potential applications in several sectors of Industry. In metallic materials, it’s a phenomenon associated to martensitic transformation and its reversion process in which the material, after being deformed over its elastic limit, can reestablish its original form through heat treatment. It’s really worthwhile to explore this feature because it allows the fabrication of joints and pipe coupling joints without the use of welds. The stainless Fe-Mn-Si-Cr-Ni alloys which this property are potentially eligible for this application and thus have attracted attention of researchers because they’re a cheap economical alternative and easy to be produced when compared to copper and Nickel-Titanium alloys with shape memory shape effect. However, these stainless alloys still feature discrete values of shape recovery and limited corrosion resistance. Besides that, the fabrication processes of this alloys reported in literature involve the production through induction vacuum furnaces that add great cost to the final product and imply few engineering applications. In this regard, the present work aimed to contribute to the study of Fe-Mn-Si-Cr-Ni alloys with shape memory effect and creating and characterizing an alloy obtained by fusion in inductions furnace without controlled atmosphere, assessing the different conditions of material processing when in terms of microstructure, mechanical properties, degree of shape recovery and corrosion resistance. The fusion of the alloy has generated an ingot with a high level of non-metallic inclusions and a certain amount of interdendritic porosity as well as the formation of secondary phases in the microstructure. The homogenization treatment has dropped some of the secondary phases, but the air cooling generated precipitation in grain boundaries. In the heat-treated condition, the alloy featured better results in terms of shape recovery and corrosion resistance.
Inibição da síntese de óxido nítrico reduz termogênese durante a inflamação sistêmica induzida por LPS em pintainhos
(Universidade Federal de São Carlos, 2014-08-29)
Dantonio Junior, Valter
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Bícego, Kênia Cardoso
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https://lattes.cnpq.br/0169967378071426
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https://lattes.cnpq.br/5692455955272475
Low and high LPS doses (endotoxin) have been used as models to study fever and endotoxic shock, respectively. In rats, systemic nitric oxide (NO) directly affects thermogenesis, but does not mediate those responses. Even though few studies in birds suggest NO as a pyrogenic signalling to brain, there is not enough evidence for confirming this suggestion. We hypothesise that, compared to mammals, systemic NO presents similar thermogenic action during endotoxin challenge in birds, even in early life. As precocial birds, chickens have a developed thermoregulatory capacity immediately after hatching, which constitutes an interesting model to study thermoregulation in early life. Thus, we investigated the role of NO in LPS-induced fever and endotoxic shock in 5-day-old chicks. The doses of 2 (LPS2) and 10 g kg-1 of LPS induced fever 3-5 hours after intramuscular (IM) injection, while 50 and 100 (LPS100) g kg-1 decreased Tc within the first hour followed by fever at 4-5 hours postinjection. Plasma nitrate levels increased 4 hours, but not 1 hour, after treatment with LPS2 and LPS100 (higher increase). There was no correlation between plasma nitrate concentrations and Tc at both LPS doses. L-NAME (non-selective NOS inhibitor; 50 mg kg-1; IM) inhibited LPS2- and LPS100-induced fever and increased the magnitude of the fall in Tc caused by LPS100. In contrast, no effect of L-NAME was observed in LPS-treated chicks in warm conditions. L-NAME decreased oxygen consumption in LPS2- and LPS100-treated chicks, but the response was more pronounced with LPS100. Moreover, LPS-induced huddling (heat conservation behaviour) was accentuated by LNAME. Our results seem to indicate that, like in rats, systemic NO is not a mediator of fever and endotoxic shock in chicks, but does affect thermogenesis in these animals, which may constitute a common effect of NO in the endothermic vertebr.
Ajustes da variabilidade da frequência cardíaca em repouso e durante o exercício em indivíduos com asma controlada
(Universidade Federal de São Carlos, 2014-08-29)
Araújo, Adriana Sanches Garcia de
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Di Lorenzo, Valéria Amorim Pires
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https://lattes.cnpq.br/9071791546812165
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https://lattes.cnpq.br/0104886088821769
Asthma is a chronic disease, with periods of exacerbation and worsening of airflow obstruction and may be triggered by various stimuli, including exercise, and is associated with dysfunction of the autonomic nervous system (ANS). In this context, two studies were conducted to assess adjustments of ANS in asthmatic volunteers in different situations. The first study was entitled: Increased sympathetic modulation and decreased response of the heart rate variability response in controlled asthma , was designed to evaluate the adjustments of indices of autonomic modulation of heart rate (HR) at rest in supine and seated positions and during the respiratory sinus arrhythmia maneuver (M-RSA). The assessment of 12 asthmatic volunteers included pulmonary function tests and measures of heart rate variability (HRV). The results suggest that in asthma appears to be an association of airflow obstruction with reduced total variability, and in addition, to postural change seems to be reduced adjustment of the sympathetic nervous system in asthmatic women. The second study entitled: Airway responsiveness at exercise recovery and adjustments of heart rate variability at rest and exercise in controlled asthma , evaluated the adjustments of cardiac autonomic modulation during exercise its association with the exercise induced bronchoconstriction. Sixteen asthmatics volunteers underwent constant speed exercise test, forced vital capacity maneuver before and after exercise testing and register of HR and R-R intervals. With this second study, it can conclude that in asthmatics, even with controlled disease, injury presents postural and autonomic adjustments during the year and reduced complexity of dynamical systems of HRV during exercise.