Browsing by Issue Date, starting with "2020-03-04"

Filter results by year or month

Now showing 1 - 20 of 11050

Estudo dos efeitos antiangiogênicos da desintegrina-símile Alternagina-C (ALT-C) em células endoteliais (HUVEC), tendo como alvo a integrina α2β1
(Universidade Federal de São Carlos, 2020-03-04)
Santos, Patty Karina dos
;
Araújo, Heloísa Sobreiro Selistre de
;
https://lattes.cnpq.br/4065824911933203
;
https://lattes.cnpq.br/1859294987166162
Angiogenesis is a crucial process in tumor progression, and it is mainly regulated by the vascular endothelial growth factor (VEGF) and its receptor, VEGFR2. Studies have shown that VEGF/VEGFR2 axis signaling is directly associated with functional responses of integrins in endothelial cells (crosstalk). Alternagin-C (ALT-C), a disintegrin-like protein from Bothrops alternatus snake venom, has high affinity for α2β1 integrin, interfering in cell adhesion, proliferation and migration, besides modulating angiogenesis in a concentration-dependent form by a not completely understood mechanism. Here we evaluate the antiangiogenic activity of ALT-C in human umbilical vein endothelial cells (HUVECs) associated or not with VEGF, as well as its interference in α2β1/VEGFR2 crosstalk. ALT-C was purified from B. alternatus venom and 1000 nM of the protein was tested in several in vitro experiments using HUVECs. ALT-C strongly inhibited HUVEC tube formation and decreased VEGFR2 and α2β1 protein levels. The disintegrin-like protein affected actin cytoskeleton, decreasing the number of cell filopodia, inhibiting HUVEC adhesion to collagen I and cell migration. ALT-C interfered in ERK 1/2, PI3K and FAK-Src/paxillin pathways, besides inducing autophagy, resulting in inhibition of the angiogenic process. Additionally, co-localization and surface plasmon resonance assays demonstrated that ALT-C interacts with α2β1 and VEGFR2, respectively, having a 10-fold higher affinity for the integrin. All these results suggest that ALT-C, after binding to α2β1 integrin, inhibits VEGF signaling by interfering in the α2β1/VEGFR2 crosstalk, which results in angiogenesis impairment. We conclude that ALT-C is a potential candidate for the development of antiangiogenic therapies for tumor and metastatic treatment.
Organização partidária e coordenação eleitoral no Brasil
(Universidade Federal de São Carlos, 2020-03-04)
Souza, Fabiana Cristina dos Santos de
;
Ribeiro, Pedro José Floriano
;
https://lattes.cnpq.br/6336151484882362
;
https://lattes.cnpq.br/4653921266247913
Faced with the importance of parties for the consummation of democratic regimes, some issues emerge in relation to their functioning, mainly related to how decisions are made within the consortia. Thus, the central objective of this research is to analyze the political parties through their internal organization, since, as Panebianco (2005) points out, the parties must, first, be understood as organizations that vow to occupy the government and the legislative. The key question that permeates the analysis is how party strategy, nationalization or provincialization, whether pure or national, focuses on the chances of a party launching nominations for governors and senators in national elections, as well as sewing agreements elections? The intention is to understand how the parties outline their electoral strategies according to the autonomy or centralization granted to subnational bodies. The proposed cut includes the six main Brazilian parties: PT, PSDB, PMDB, PSB and DEM, analyzing the elections that took place in 2010, 2014 and 2018 for governors and federal deputies of the 27 Brazilian states. The hypothesis is that the launch of presidential candidacies corroborates the adoption of a presidential strategy, causing the national summit to centralize the process of launching candidatures for Governor and Senator, seeking support at the national level. Thus, parties directly involved in the PT and PSDB presidential elections together with the DEM, which traditionally is a source of support for the latter, and the traditional PT partner PSB would impose further restrictions on the state summit by establishing certain options for partisan coalitions in the states as well as in the launching of applications, in view of the national scenario. The PMDB seeks through the state elections to nationalize their votes, which incurs greater autonomy at the state levels of the party. To test the hypothesis will be observed the electoral resolutions of the parties, which dictate the main guidelines that guide them in the national elections.
Produção científica do periódico Cadernos Brasileiros de Terapia Ocupacional no período de 2012 a 2017: um estudo bibliométrico
(Universidade Federal de São Carlos, 2020-03-04)
Santarpio, Éder José
;
Hayashi, Carlos Roberto Massao
;
https://lattes.cnpq.br/9136586124586219
;
https://lattes.cnpq.br/3135584595001001
Introduction: Scientific communication is an important stage in the production of knowledge, as it is through it that the process of knowledge transmission occurs. Among formal communications, scientific journals occupy a prominent place, as it is one of the main and most widespread means of scientific communication. Because it is one of the main means of scientific communication, the scientific journal becomes an object of extreme importance for bibliometric studies. Bibliometrically evaluating a given scientific journal allows to reveal, by obtaining indicators, certain characteristics that will serve as a basis for the representation of the scientific field linked to the journal. Objective: the study has as its general objective to map knowledge production in Occupational Therapy in the journal Brazilian Journal of Occupational Therapy, between the years 2012 to 2017, through the bibliometric approach. Methodology: Based on the objectives, the research can be classified as exploratory and descriptive with a quantitative and qualitative approach (multimethod). The research was developed in the following stages: 1) Construction of the theoretical framework - Scientific communication; the role of journals in the formal scientific communication process; bibliometry; Science, Technology and Society. 2) Elaboration of the collection protocol and the definition of the technological resources used in the study. 3) Data collection: Data were collected for 417 articles, 26 articles from dossiers and 25 editorials published in the journal Brazilian Journal of Occupational Therapy. 4) To store the data, Microsoft Office Excel® and Microsoft Office Word® spreadsheets were used. Vantage Point® software, scriptLattes and Microsoft Office Excel® were used to process the collected data. Results and Conclusions: It was found that the area has multidisciplinary characteristics, but most are linked to the large area of Health; the predominance of females in the area of Occupational Therapy was confirmed; a collaborative authorship process was identified; with regard to the authors' productivity, it was possible to verify that there is a low dispersion in relation to their productivity. It was possible to identify the internationalization of the journal and, as a consequence, the influence of foreign research on it; the results revealed that the majority of authors are linked to Public Institutions of Higher Education. We can conclude, based on the data of this research, the relevance of this journal for the area of Occupational Therapy in Brazil.
Desenvolvimento de processos tecnológicos para preservação e descontaminação de alimentos
(Universidade Federal de São Carlos, 2020-03-04)
Perez, Shirly Marleny Lara
;
Blanco, Kate Cristina
;
https://lattes.cnpq.br/9029359807019219
;
Bagnato, Vanderlei Salvador
;
https://lattes.cnpq.br/4947860249518663
Contamination of foods caused by microorganisms occurs through their handling and or processing, which can change their physical-chemical characteristics and decrease shelf life, and cause infection. The objective of this work was to evaluate the effect of gaseous ozone (O3) and to the combination of ultraviolet light and aqueous ozone, to analyze the best protocol that makes it available to inactivate bacteria contained superficially on beef with in natura contaminations and by Escherichia coli. A prototype for gaseous ozone was designed, and the evaluation in inert samples submitted to gaseous ozone decreased around 3,88 Log (CFU / cm2) of E. Coli in 30 minutes, and for beef samples with in natura contamination, the microbial reduction was 50% after 10 minutes. Experiments were conducted to evaluate the microbiological behavior of the meat simulating exposure situations in the 12-hour butcher shop, the results showed that in the 30 minutes exposure time there was a reduction of 2,18 Log (CFU / cm2), and in the second refrigeration the load was inactivated, however, the control sample under equal temperature conditions and without treatment had a proliferation of 1,7 Log (CFU / cm2). To evaluate the combined treatments of ozone in aqueous phase and ultraviolet light, the equipment was built and tested against E. coli on contaminated beef. Samples were added to 10 repetitive cycles for the combined and isolated treatments. For the isolated treatment of ultraviolet light, a reduction of 1.3 Log (CFU / cm²) was obtained at the end of cycles and for the ozone treatment, there was no significant difference in microbial inactivation (p <0,05), but there was a difference for the final control sample of the experiment, which grew by 0,6 Log (CFU / cm²), the combined cycles showed similar inactivation of 0,7 Log (CFU / cm²) approximately and all combined and isolated treatments showed food preservation avoiding the exponential proliferation of microorganisms. The organoleptic properties of the meat were evaluated after the treatments, the pH was checked, the protein quantified by the Bradford method, and TBARS tests were performed for both prototypes and gaseous ozone, histological slides were also analyzed for structural damage and water holding capacity. Organoleptic evaluations did not show significant differences about the control samples, for both technologies; gaseous ozone and combination of ultraviolet light and aqueous ozone and showing potential for decontamination of food without deteriorating its quality.
A dinâmica da ciência e da tecnologia nos intercâmbios entre Brasil e China: o caso dos Institutos Confúcio
(Universidade Federal de São Carlos, 2020-03-05)
Martinelli, Marina
;
Andrade, Thales Haddad Novaes de
;
https://lattes.cnpq.br/5882842888759363
;
https://lattes.cnpq.br/9368198175936317
THE DYNAMICS OF SCIENCE AND TECHNOLOGY IN EXCHANGES BETWEEN BRAZIL AND CHINA: THE CASE OF CONFUCIO INSTITUTES IN BRAZIL This work addressed the topic of academic exchange between Brazil and China, specifically the case of Confucius Institutes in Brazil, through the investigation of the experiences of UNESP and UNICAMP. The objective was to understand how CIs contribute to academic exchange in the area of economic and cultural cooperation between Brazil and China and how these particular relations contribute to the social stratification of those two countries, affecting Brazil's social structure in particular. In the other hand, understanding how the dynamics of Science and Technology works in the production of a globalized elite that will lead a new way of acting, which will influence the direction of the future of life in society. The instruments through which these young people are working and how to democratize this category were studied through the Confucius Institutes. In this research, bibliographic and bibliometric research was done; the websites of the CIs of UNESP and UNICAMP were analyzed; documents produced by the CIs were analyzed; a questionnaire was applied for semi-structured interviews with the directors of CIs, key people and exchange students, through a multi-method methodology, which combines quantitative and qualitative researches, through a macrophysical and microphysical look. The justification is found in the political, cultural and economic movement provided by Brazil - China relations, through the BRICS. The Ciência Sem Fronteiras, Idioma Sem Fronteiras and Paraná Fala Inglês programs were studied because they are the most recent models of Brazilian exchange, although CIs are closely linked to programs such as those of the Goethe and Cervantes Institute, only institutionally, rooted in the universities. Brazil has 12 CIs and none of them intends to indoctrinate Confucianism, but rather to spread the Mandarin language, therefore, the experience has demonstrated the importance of a truly bilateral assessment of tasks in the face of Chinese soft power.
Caracterização de rizobactérias associadas à Solanum lycocarpum St. Hil. quanto ao potencial biotecnológico para promoção de crescimento vegetal, enzimas e agentes antimicrobianos
(Universidade Federal de São Carlos, 2020-03-05)
Lisboa, Paulo Henrique Gomes
;
Sousa, Cristina Paiva de
;
https://lattes.cnpq.br/9002619114161319
;
Lacava, Paulo Teixeira
;
https://lattes.cnpq.br/8947678349043750
Solanum lycocarpum St. Hil., endemic plant of the Brazilian tropical savanna, known as the “Cerrado”, has been studied for its medicinal properties and for having the ability to grow in acidic and nutrient-poor lands, attracting interest in the investigation of its rhizospheric microbiota, which includes plant growth-promoting rhizobacteria (PGPR). Industrial enzymes and antibiotics can also be obtained from them. In this work, the biological nitrogen fixation capacity was tested in NFb semi-solid culture medium and 32.6% of the isolates showing positive results. In the inorganic phosphate solubilization tests, 45.8% of the isolates demonstrated this capacity in nutrient agar supplemented with insoluble phosphate. The solubilization index (SI) for calcium phosphate by the bacterial isolates grown in solid medium varied from 0 to 4.833. All bacteria produced indole acetic acid (ranging from 0.07 μg.mL-1 to 134.1 μg.mL-1) in Tryptic Soy Broth (TSB) 10% + L-tryptophan medium. In assays against pathogens, antagonistic activity was demonstrated against a spectrum of fourteen phytopathogenic fungi through the technique of direct pairing in Potato Dextrose Agar (PDA) medium and three clinical pathogens using the agar block method. The bacterial isolates were striated in special mediums whose carbon sources were substrates of the enzymes in the enzyme activity assays and 88.4% of them had at least one of these activities. The best isolates were identified by partial sequencing of the 16S gene as belonging to the genera Bacillus, Burkholderia and Microbacterium.
A dimensão relacional entre curadoria digital e metadados
(Universidade Federal de São Carlos, 2020-03-05)
Triques, Maria Lígia
;
Arakaki, Ana Carolina Simionato
;
https://lattes.cnpq.br/9896600626524397
;
https://lattes.cnpq.br/9360108532923377
The amount and variety of data associated with cultural heritage have grown significantly in digital environments, generating a diversity of possibilities and challenges related to information representation, especially in studies focused on digital curation. In the Web environment, there is need for data informational treatment to take into account, in addition to all the stages through which they circulate, the ability to guarantee their informational purposes. In this scenario, digital curation is highlighted as an interdisciplinary activity that seeks to establish guidelines not only to maintain and preserve informational resources but also to ensure that data are continuously consistent with their informational purposes. To achieve this goal, digital curation adds value to data through the representation of information from metadata. Thus, this study questions how metadata influence digital curation actions and aims analyze the relationship between metadata and digital curation. This theoretical qualitative study was carried out through bibliographic survey and exploratory systematization, also using a documentary study of the Europeana platform to exemplifying digital curation and data representation, in this case, cultural heritage data made available on the Web environment. Results showed that digital curation seeks, through the process of adding informational value, to enrich data in such a way as to facilitate sharing and reuse. Therefore, it was found that metadata influence digital curation actions to the extent that these elements enable explanation of the semantic definitions necessary for communication within the management process, mainly in an automated way. In the end, the study about the Europeana platform provided the basis for the results, demonstrating how metadata, through instruments, principles, models and technologies, allows the semantic context of informational resources of cultural heritage to be ensured. In conclusion, the importance of metadata for digital curation is perceived not only as an essential factor to guarantee planning and execution of its actions, but mainly to allow digital resources to have their informational purpose assured and remain with quality and reliability for long-term access, use and reuse.
Habilidades sociais e adaptação da criança à pré-escola: concepções e habilidades sociais de educadores
(Universidade Federal de São Carlos, 2020-03-05)
Alho, Naara de Oliveira Gonçalves
;
Del Prette, Zilda Aparecida Pereira
;
https://lattes.cnpq.br/8113238388739093
;
https://lattes.cnpq.br/9891697288291700.
School enrollment is a crucial moment in child development. The adaptation process in this space has been the object of theorization and research. School adaptation at this stage was understood from Bronfenbrenner’s concept of ecological transition, as a process of acquiring new social roles and affective bonds, established in order to provide mutual trust, favoring and influencing development, in addition to experiences that allow the formation and maintenance of psychic, cognitive and social skills. An important resource in adaptation is the child’s social skills repertoire to make friends, demonstrate empathy, self-control, emotional expressiveness, civility, assertiveness, solving interpersonal problems. In Brazilian literature, there are still few studies that have investigated the child’s adaptation period to school, particularly regarding the influence of social skills on the child’s insertion in school. The objectives of this study were to characterize the repertoire of social skills of children in pre-school age, to establish the relationship with indicators of adaptation to school, as well as to identify favorable and unfavorable indicators for school adaptation with an emphasis on teacher’s educational conceptions and social skills. Three schools were selected, two public ando one private, 4 teachers from these institutions and 10 children aged between 4 and 4 years and 6 months. For data collection, a Protocol for Observation of School Adaptation (PROAE) was prepared, which was completed by the teachers during the child’s first 4 weeks at school and in the first 3 months. The teachers and guardians of the children responded to the Scale of Social Behaviors of Preschoolers (PKBS-BR). Teachers and parents also answered a questionnaire with questions about how they conceived school adaptation and the Inventory of Social Educational Skills (IHSE-Prof). The data were computed in scores, organized in SPSS 25.0 spreadsheets and analyzed using descriptive and inferential statistics. The results indicated that children who had problems with school adaptation over 4 weeks and in the general evaluation of 3 months had worse repertoire of social skills and their teachers had a deficit or a lower average in terms of educational social skills, this data corroborates with some studies in the literature that have shown that children with a worse repertoire of social skills, have more problems for psychosocial adjustment. The responses of teachers and parents about school adaptation differed in some aspects, such as, for example, regarding the concept of school adaptation, parents associated adaptation as something geared to the child’s ability to adjust to the new environment, to behave well and demonstrate emotional well-being, while the teachers included their own performances and abilities to deal with this period. Some methodological and educational implications of the results were discussed, suggesting intervention planning based on them.
A criança como leitora, suas representações e significações: práticas de letramento em uma sala de educação infantil na Unidade de Atendimento à Criança – UAC - UFSCar
(Universidade Federal de São Carlos, 2020-03-05)
Hermann, Amanda dos Reis
;
Zuin, Poliana Bruno
;
https://lattes.cnpq.br/0389350936548546
;
https://lattes.cnpq.br/8719958510398247
The present Master's research aimed to investigate the ways in which the child in Early Childhood Education, aged between 3 and 4 years of age, constitutes itself as a reader through literacy practices experienced during school routines. The specific goals were: I. To identify how this process occurs, through literacy practices established by the class’ teacher, such as reading and conversation circles, dialogue with the children, contact with the various discourse genres, projects and world experiences; II. To analyze how they read and what they understand; III. To check how the reading of images and the retrieval of already read stories are retold by them; IV. To investigate which linguistic marks are used by them in the story telling of different discourse genres, and V. To investigate which the teacher’s actions provide literacy in a child of during the small childhood. Our theoretical reference is based on the Philosophy of Language (BAKHTIN, 2003, 2011), Historical-Cultural Psychology (VYGOTSKY, 1982, 1996, 2005, 2009; LURIA, 2000, 2005; LEONTIEV, 2005), Critical-Libertarian Pedagogy (FREIRE , 2005, 2009, 2011) and studies about Literacy (KLEIMAN, 1995, 2005, 2009; ROJO, 1995; TFOUNI, 1995; SOARES, 2003, 2010, 2017). In order to answer the objectives proposed in this study and based on the theoretical reference mentioned above, a field research with a qualitative focus was carried out. 12 insertions were made in a classroom at the Child Care Unit - UAC-UFSCar, with children between the ages of 3 and 4, during the second semester of the year 2018. To constitute the corpus of this work, the following data collection instruments were used: observation in locus, registers, filming, photographs and analysis of documents. The data analysis pointed out that the children signified and re-signified the readings based on literacy practices carried out by the teacher, and they had as mediator elements: memory reading, imitation reading, taking as a model those who had read to them, the oral retelling, the image reading and the reading by interpretation and the world reading. It is interesting to point out that the readings also occurred in the representations through games, social roles and drawings.
Avaliação da secagem convectiva de resíduos da indústria cervejeira
(Universidade Federal de São Carlos, 2020-03-05)
Pinto, Guilherme Henrique Alves
;
Freire, Fábio Bentes
;
https://lattes.cnpq.br/8937961078558996
;
https://lattes.cnpq.br/6919620534872525
Brazil is the third largest beer producer in the world, with an estimated production of 14.1 billion liters of the product for the year 2018, generating several co-products throughout the process, being brewer’s spent grain and spent yeast the main ones. The reuse of these materials is more interesting from the economic and logistical point of view in relation to the usual destination, however it is necessary to understand their drying to make their new destination viable. The present work aimed to evaluate the operational feasibility of reducing the humidity of both materials through convective drying, applying different dryers for each co-product. To malt bagasse drying was used a system consisting of a rotary pan with a capacity of 10 liters associated with a blower responsible for the hot air supply. It was evaluated how the drying kinetics, temperature profile, specifically the temperature on the inner surface, Tw, and the spent grain temperature, Ts, and the solids circulation intensity were affected by the dryer operational conditions, specifically the air temperature at the blower outlet, TaE, the spent grain mass added to the system, ms, and the pan rotation, Ωdrag. For this purpose, two different values were used for each parameter, TaE=67.7 and 101.2 °C, ms,0=1.0 and 1.5 kg, and Ωdrag=25.1 and 33.9 rpm. The drying tests were carried out until the dynamic equilibrium moisture content was reached. It was necessary 180 minutes of drying to achieve dynamic equilibrium with TaE = 101.2 °C and ms,0=1.0 kg, while for TaE=67.7 ° C and ms,0=1.5 kg were necessary 420 minutes of drying, being noticed a significant influence of TaE and ms,0 on the kinetics. By modifying Ωdrag maintaining TaE and ms, dynamic equilibrium was achieved at the same drying time, with no significant influence of Ωdrag on the process. The solids circulation, Ωs, presented a parabolic profile with a minimum value of approximately 16 rpm at the beginning and end of drying and a maximum value of approximately 27 rpm in the intermediate periods. As the dryer rotation remains constant throughout the operation, it was concluded that the bed properties modifications, mainly spent grain moisture, induced the profile observed for Ωs. Tests were carried out using the centrifugal method to relate the adhesion force between the dryer surface and the spent grain with its moisture content, being observed forces of greater intensity for the material in its original conditions and practically null forces with a completely dry material, behavior commonly observed in materials in which the capillarity effects predominate in surfaces contact. The temperature profile showed a behavior that could be divided into three distinct periods, a period corresponding to the beginning of drying, in which the dryer surface and spent grain were in thermal equilibrium, with Tw approximately equal to Ts, an intermediate period, in which Tw was greater than Ts, and a third period corresponding to the end of drying, in which Tw was lower than Ts. It was possible to conclude that the adhesion between the spent grain and the surface also caused this temperature behavior, especially in the first period, when the stronger contact between the phases by capillarity provided the thermal equilibrium. It was concluded that the spent grain adhesion to the dryer surface affected its operation, especially in the particles circulation and the temperature profile. In the spent yeast drying, a vibrofluidized bed dryer was used, fixing the vibrational conditions, A=0.015 m and f=500 rpm, and the paste feeding at 14 mL/min, varying the drying air temperature at 80 or 100 °C, the mass of glass spheres at 0.5 or 1.5 kg and the superficial velocity of the fluid at 1.5umf and 1.2umf to evaluate the effect of these parameters on product formation, material accumulation, fluid dynamics of the bed and product quality. Each drying test was conducted for 30 minutes, followed by 30 minutes with no paste feeding for the dry material detachment. There was a change in the fluid dynamics curve for different inert masses, with a reduction in the minimum fluidization velocity and in the pressure drop with a lower static bed height. For 1.5 kg of inert, 80 °C and 1.2 umf, no product formation was observed, unlike the other tests with different operational conditions, evidencing their influence on the production rate, the highest rate, 0.263 g/min of dry poder, was observed using na inert mass of 1.5 kg and air feeding at 1.2 umf and 100 °C. However, the production rates achieved were considerably small compared to the paste feed. At the end of drying, it was possible to observe a low detachment of material from the inert surface, maximum 0.532 g/min. Thus, most of the material fed into the dryer remained attached to the inert and chamber wall, indicating that the material detachment limited the operation. Drying was sufficient to reduce the moisture content, from 90.63% on a wet basis to a maximum of 8.12%. For each test, the product moisture content for 10 to 20 minutes of drying was higher than that obtained in the initial and final 10 minutes. It was not possible to obtain a clear relationship between the product’s cell viability and the operational conditions.
Currículo e Base Nacional Comum Curricular: o processo de implementação na rede estadual paulista
(Universidade Federal de São Carlos, 2020-03-05)
Nascimento, Rodrigo Rios
;
Furlan, Elaine Gomes Matheus
;
https://lattes.cnpq.br/3179865576965852
;
Faria, Paulo Cezar de
;
https://lattes.cnpq.br/3630521378837174
This research aims to analyze the Brazilian learning standards known as Base Nacional Comum Curricular (BNCC) – following as a normative document that guides the formulation of curricula - and its implementation process in the public education network of the state of São Paulo, as well as the elaboration of the São Paulo curriculum (CP), which emerged as its unfolding. The theoretical reference is based in Sacristan (2017), which understands the curriculum in a procedural dimension, that links practices and configures them, presenting curricular concepts such as cultural selection, the prescribed curriculum and the curriculum offered to teachers. The method included a documentary survey (Gil, 2007) based on the BNCC and the Curriculum of the State of São Paulo, and the analysis of semi-structured interviews with teachers, managers of school boards and public schools. It is based on the textual discursive review (Moraes and Galiazzi, 2016) to understand the interviewees ' lines on aspects of BNCC and CP. It was possible to observe that BNCC, as a normative document, can guide the formulation of regional curricula, as mentioned in CP confirming the hypothesis of this investigation; and have implications on the production of educational material that supervises the pedagogical practice, by the Programa Nacional do Livro e do Material Didático (PNLD). In conclusion, the process of implementation in the public education network of the state of São Paulo may not be satisfactory in virtue of the comprehension and expectations from some educational agents about the BNCC and the CP, itself. However, the topic has high relevance in the Brazilian education scenario because it is a latent and urgent topic, considering that the BNCC will have implications in the (re)reorganization of regional and local curricula in a country with great regional diversities. This research aims to analyze the Brazilian learning standards known as Base Nacional Comum Curricular (BNCC) – following as a normative document that guides the formulation of curricula - and its implementation process in the public education network of the state of São Paulo, as well as the elaboration of the São Paulo curriculum (CP), which emerged as its unfolding. The theoretical reference is based in Sacristan (2017), which understands the curriculum in a procedural dimension, that links practices and configures them, presenting curricular concepts such as cultural selection, the prescribed curriculum and the curriculum offered to teachers. The method included a documentary survey (Gil, 2007) based on the BNCC and the Curriculum of the State of São Paulo, and the analysis of semi-structured interviews with teachers, managers of school boards and public schools. It is based on the textual discursive review (Moraes and Galiazzi, 2016) to understand the interviewees ' lines on aspects of BNCC and CP. It was possible to observe that BNCC, as a normative document, can guide the formulation of regional curricula, as mentioned in CP confirming the hypothesis of this investigation; and have implications on the production of educational material that supervises the pedagogical practice, by the Programa Nacional do Livro e do Material Didático (PNLD). In conclusion, the process of implementation in the public education network of the state of São Paulo may not be satisfactory in virtue of the comprehension and expectations from some educational agents about the BNCC and the CP, itself. However, the topic has high relevance in the Brazilian education scenario because it is a latent and urgent topic, considering that the BNCC will have implications in the (re)reorganization of regional and local curricula in a country with great regional diversities.
A inativação química do córtex cingulado anterior e da ínsula reverte a ansiedade em camundongos expostos ao modelo de empatia induzida pela nocicepção
(Universidade Federal de São Carlos, 2020-03-05)
Cezar, Giovana Benassi
;
Souza, Azair Liane Matos do Canto de
;
https://lattes.cnpq.br/2352004564367849
;
https://lattes.cnpq.br/7886063932893257
Empathy is characterized by the ability to recognize and respond to the emotional signals of others, being a crucial factor in the emotional experience and social interaction, allowing humans, as well as other species, to detect affective states of others. In this sense, recent studies have shown that cohabitation with an animal with chronic pain induces increased nociception and anxiety in the partner, that is, in the observer. These results reinforce the assumption that responses related to anxiety make it possible to identify the existence of emotional responses, which may be related to empathy. Evidence has suggested the involvement of the anterior cingulate cortex (ACC) and insula in the modulation of anxiety. However, no study has evaluated whether these structures participate in the increased anxiety factor observed in mice living with their partner with chronic pain. Thus, the aim of this study was to investigate the effects of reversible inactivation of ACC and insula on the modulation of anxiety induced by the empathy model in mice exposed to the elevated plus-maze (EPM). For this, male Swiss mice (n = 10-12/group), 21 days after birth, were housed in pairs to establish familiarity. On the 14th day of cohabitation, one of the animals of the pair underwent surgery for sciatic nerve constriction (NC) or surgery without nerve constriction (Sham). On the 23rd day, cagemates were submitted to stereotactic surgery for implantation of guide cannulas directed to the CCA (experiment 1) or to the insula (experiment 2). On the 28th day, cagemates (CNC and CS) that lived with their respective NC and Sham groups received bilateral intra-CCA or intra-insular saline or cobalt chloride (CoCl2) (reversible and non-selective synaptic blocker) injections, and after five minutes they were exposed to the EPM. The t test for independent samples showed that in Experiments 1 and 2, the NC mice submitted to the hot plate test showed decreased paw withdrawal latency when compared to the Sham mice. The two-way analysis of variance (ANOVA) followed by the Duncan test revealed that cohabitation with nociception mice produced an increased anxiety response in observers, confirming previous results in the literature. The chemical inactivation of the CCA and the insula produced an increased percentage of entries and time spent in the open arms, without altering the entries in the closed arms of the LCE. Furthermore, it also decreased the display of risk assessment behaviors, such as percentage of time in the center, percentage of head-dipping and percentage of protected stretched attend postures and total of stretched attend postures in experiment 1; and in experiment 2, decreased the percentage of head-dipping and protected stretched attend postures, and the total of head-dipping and stretched attend postures, which suggests a decrease in anxiety-like behaviors. The results suggest that the inactivation of the CCA and the insula reverses the display of anxiety-like behaviors in mice submitted to the empathy model evaluated in the LCE. In addition, they indicate that these structures are essential for the display of anxious behaviors induced by emotional contagion, assessed through cohabitation with animals subjected to neuropathic pain.
Oficina de língua inventada e o ensino de conceitos linguísticos
(Universidade Federal de São Carlos, 2020-03-05)
Girardi, Jane Eder
;
Conde, Dirceu Cleber
;
https://lattes.cnpq.br/2630773088169073
;
https://lattes.cnpq.br/3939612232315995
This dissertation aims to explore the possibility of teaching linguistic concepts by using as a method the creation of a language by speakers. The objective of this research is to verify, initially, what would be the advantages and disadvantages of this possibility. For this, students from the 6th and 7th grades of a public school in the city of São Carlos participated as subjects in the experiment. The experiment was applied in two different groups, with different approaches and activities, so that the results were different. In the first application, the students did not receive any direction to create their fictional language. The results were essential to the consideration of the second part. The part II of this research has been directed in the activities and discussions about languages. Thus, the data from both parts of the research were analyzed and compared, namely: students' language intuitions. We realize from this research that students do not distinguish spoken language from written language, that is, for them, both have the same language system. Therefore, in the second part, the difference between speech and writing was discussed to guide the students regarding language elaboration. Moreover, it was noted that the languages of the first phase were less economical than those of phase II. This is due to the concept that students have about language, because language is synonymous with writing for them, and writing usually assumes that there is some difficulty that encompasses it, because to learn it, it is necessary to master the rules of language cultured standard norm. To dispel this stigma, we tried to show students that every speaker has a language intuition, that the speaker is born with an internalized grammar and that it is possible to understand the linguistic aspects and phenomena about languages. Therefore, it was proposed the activity of creating a language, which aims, at this early stage, only to collect and analyze the data to investigate whether through this activity the student's intuition can emerge.
A aventura narrativa da Telltale Games: um estudo de caso de Game of Thrones – A Telltale Games Series
(Universidade Federal de São Carlos, 2020-03-05)
Bisson, Camila Brandão
;
Andrade, Leonardo Antônio de
;
https://lattes.cnpq.br/3921612856948891
;
https://lattes.cnpq.br/2717863969349057
Adventure games, as a genre defined in the context of electronic games, have had throughout their history evolutions and transformations that allowed more, and more, complex stories to be told through video games. The objective of this work starts from a macro analysis, bringing the history of adventure games as a genre, and then, in a second moment, analyzes the style and trajectory of one of the main companies recognized as a reference in the production of narrative games, Telltale Games, starting from a panorama that begins in the history of its predecessor, LucasArts, and ends in the analysis of the particular characteristics of the games produced by the company. From this context, the objective is to carry out a specific analysis of one of the company's titles: Game of Thrones - A Telltale Games Series. It is with the construction of such an analysis that it will be possible to understand how adventure games have effectively evolved as a genre and how narrative and gameplay come together around building an experience based on the player's choices and the development of the story told by the game in question.
Efeito do tratamento com hidroxiureia sobre a expressão gênica de ATF4, MSN, PBXIPI e SNCA em pacientes com anemia falciforme e em células K562
(Universidade Federal de São Carlos, 2020-03-06)
Palombo, Fernanda Cristina
;
Cunha, Anderson Ferreira da
;
https://lattes.cnpq.br/0329741640375661
;
https://lattes.cnpq.br/6201302531057089
Our study aimed to analyze the expression and protein content of four genes (ATF4, MSN, PBXIP1 and SNCA) by qPCR and Western Blotting in individuals with sickle cell anemia treated and not treated with hydroxyurea (HU) in order to establish associations with pathophysiology sickle cell anemia and, mainly, with HU treatment. To prove the results, analyzes were also performed with K562 cells culture stimulated for erythroid differentiation in the presence and absence of HU. Our results show decrease in the expression of MSN in individuals with the disease, which can be justified by the increase in cell adhesion in this group. The data obtained also suggest that treatment with HU induces the expression of PBXIPI and inhibits SNCA. The PBXIPI activation would assist in stimulating the differentiation and proliferation of hematopoietic cells, while the decrease in the content of SNCA may be associated with the reduction of inflammatory processes, which is an effect of great importance in worsening the disease. For the ATF4 gene, associated with oxidative stress and neovascularization, we expected that treatment with hydroxyurea would lead to a reduction in expression, however, we did not obtain a statistically significant difference in the comparison between groups. Finally, we suggest that the increase in oxidative stress by adding H2O2 to the culture medium also influences the expression of our genes and affects the potential effect of hydroxyurea.
Alienação parental: revisão sistemática de estudos documentais e análise da aplicação do conceito em sentenças judiciais
(Universidade Federal de São Carlos, 2020-03-06)
Oliveira, Ricardo Pereira da Silva
;
D'Affonseca, Sabrina Mazo
;
https://lattes.cnpq.br/0381029115416584
;
https://lattes.cnpq.br/3904654445289766
The phenomenon of Parental Alienation (PA) has been identified more frequently in the context of child custody disputes by parents in courts of law, especially after the enactment of Law no. 12,318 (2010) (Parental Alienation Law). In this context, this dissertation sought to understand how the judicial system has evaluated and responded to suspected cases of PA. For this purpose, two studies were carried out, the first with the objective of conducting a systematic review of national and international documentary studies with judicial samples involving AP and the second with the objective of analyzing judicial sentences of Family Courts of the Court of Justice of the State of São Paulo (TJSP) with charges of AP. The systematic review was carried out following the guidelines of the PRISMA protocol. The keywords "parental alienation" and "parental alienation", were searched in 4 databases. The book collection of the Laboratory for Analysis and Prevention of Violence was also consulted. Six studies were selected, of which 4 were Brazilian, 1 Canadian and 1 Italian, demonstrating that there are still few studies on AP that use samples of judicial origin. The revised literature indicates that the justice system has based most of its decisions on PA cases in the conclusions of psychological reports produced by court experts. However, the reviewed studies identified deficiencies in the psychological assessments of suspected PA. The documentary study, on the other hand, carried out a search in an electronic tool for accessing TJSP information, resulting in 217 sentences that mentioned the term “parental alienation” between 2010 and 2019. 128 sentences were selected for analysis. Among the results, the participation of expert psychologists stands out for the outcome of the process, since their reports served as a basis for 75% of the sentences, with the magistrates showing confidence in their conclusions, which calls attention to the psychologists’ responsibility for the protection of children involved in marital litigation. Most of the accused parents of AP were female guardians. Only in 19 sentences was someone declared alienator, usually women. They were also the target of false accusations more frequently than men, indicating that PA accusations were generally used as an instrument of gender-based violence. From the analysis performed, it was possible to describe an overview of how PA cases are analyzed, substantiated and judged in the State of São Paulo. Finally, the present dissertation allows us to understand how the evaluation and judgment of PA accusations takes place. The results found were similar to those of other researches in the area, indicating trends that overcome cultural barriers. It should be noted that the assessment of the occurrence of PA proved to be complex and challenging, requiring a strong professional commitment from the technicians and magistrates involved, considering that the family dynamics are diverse, deserving an individualized assessment of each litigation so as to contemplate its particularities at the same time that privileges the rights of the children involved.
Comparação do desempenho de diferentes estratégias de montagem do genoma de Bertholletia excelsa Bonpl. (Lecythidaceae)
(Universidade Federal de São Carlos, 2020-03-06)
Barros, Laís Rossetto Ferraz de
;
Martins, Karina
;
https://lattes.cnpq.br/9367637840430435
;
https://lattes.cnpq.br/3170045878886584
The Brazil Nut (Bertholletia excelsa) is an arboreal species, native to the Amazon forest, by which there’s a concern referring to its ecological sustainability. Therefore, sequencing and assembling the genome might contribute to the conservation of genetic diversity and maintenance of natural populations, besides assisting the comprehension of the success of future populations. Therefore, the objectives are to assemble the genome of Bertholletia excelsa, compare different assembly methodologies, and define the methodology that results in the best quality. The methodology consisted in sequencing DNA samples from an adult individual of Bertholletia excelsa with Pacific Biosciences is Single Molecule Real Time. This step resulted in a genomic coverage of 187× and a N50 of raw reads of 14,02 kb. Following up, the haploid genome size was estimated from k-mer distribution (k=22), resulting in ~596Mpb. Subsequently, there were tested five assemblers (wtdbg2, MECAT2, SMARTdenovo, Flye and Canu) with six genomic coverages (47×, 63×, 97×, 126×, 187× and 60× with corrected reads by Canu). QUAST was used to compare the efficiency of these methods through the following parameters: contig N50, number of contigs, L50 and assembly size in relation to genome size estimative. BUSCO was used to determine the genomic completeness. The LAI score validated its quality, considering the contiguity and integrity of repetitive sequences. In conclusion, the best assembly was SMARTdenovo with genomic coverage of 187×, resulting in a contig N50 of ~2,6 Mb, genic completeness of 95,1% and LAI score of 10,53; totaling an assembly of 649.349.366 pb.
Conquistando o ambiente urbano: valor adaptativo e comportamento parental nos ninhos de Turdus leucomelas (Aves, Turdidae) em edifícios
(Universidade Federal de São Carlos, 2020-03-06)
Batisteli, Augusto Florisvaldo
;
Ferreira, Marco Aurélio Pizo
;
https://lattes.cnpq.br/1663295770796752
;
Sarmento, Hugo
;
https://lattes.cnpq.br/4515469289999439
Reproduction is a crucial stage of the life cycle of all species, and the related energetic conflicts are especially important on species that present intense parental care, such as altricial birds. Nest site selection is directly linked to adult fitness by potentially minimizing the costs of offspring rearing and predation risk for both adults and young. However, the influence of anthropogenic environments on bird behavior and reproduction is still poorly known. Here, I address the effect of trees and buildings as nesting substrates on the incubation behavior and clutch survival in the Pale-breasted Thrush (Turdus leucomelas). I also assess the causes and consequences of mate change and nest reuse, and the relationship between the use of buildings as nesting substrates and female personality. Clutches on buildings had higher success due to a protective effect of buildings during the egg phase. The internal temperature of nests was higher on buildings, creating a compensatory effect for the incubation recesses took by females along the daytime. Consequently, females of nests on buildings showed lower nest attentiveness due to longer incubation recesses. I also found that pairs that stayed together had higher chance of nest reuse, and produced more fledglings. Nests were more often reused after a successful clutch, especially within the same breeding season. Nest reuse, in turn, enhanced earlier clutches at the beginning of the breeding season, and reduced the chance of brood parasitism by the Shiny Cowbird (Molothrus bonariensis). Finally, female personality differed between nesting support types, with those that nested on buildings were less neophobic. The results suggest that pairs that nest on buildings may have a higher fitness, by either lower incubation costs and higher clutch survival, the last also related to the increased chances of nest reuse and its advantages. Additionally, the difference on the personality of females that nested on buildings indicates that the advantages of exploiting that anthropogenic resource are dominated by a specific subset of the population. In conclusion, the use of buildings as nesting substrates increase the breeding performance of our study species in the urban environment in multiple ways, contributing to the adaptation of this species to this environmental context.
A edição brasileira do objeto editorial “S.”: uma leitura do paradoxo de O Navio de Teseu
(Universidade Federal de São Carlos, 2020-03-06)
Doretto, Vitoria Ferreira
;
Salgado, Luciana Salazar
;
https://lattes.cnpq.br/5206927424265495
;
https://lattes.cnpq.br/6900992998539019
In this study we analyze the Brazilian edition of S., by JJ Abrams and Doug Dorst, as a medium (DEBRAY, 1993; 1995; 2000), a confluence of modes of material inscription and forms of circulation made by the combination of institutions that endorse the speeches, that produce technical objects that support them, that is, a sensitivity vector that points to a sociability matrix. We show how this editorial object points to its sociability matrix, Editora Intrínseca, which participates in a complex system of constituent elements of the editorial field, dealing with its material formalization (FLUSSER, 2007), describing the elements that relate to the material dimension of the object, highlighting aspects of the organized material to approach the book as a vector of sensitivity. We describe the details of the book's constitution, that is, the editorial genetic rites (SALGADO, 2016) involved so that it could go public. We also presented the marketing actions of the original edition and the Brazilian edition of the book, differentiating the type of approach taken by the publishers Mulholland Books and Intrínseca to show how the Brazilian publisher bet on the use of Abrams' image and fame and how they reinforced the idea that the book is, in fact, a puzzle book, a literary experience that could be read with the same expectations as to when watching a production by the American filmmaker. When we verified how the publicization of S. took place, how it was presented, and how it is a medium, we realized that its materiality and amalgamated circulation produce a reception phenomenon that gives it value. So, as we are interested in the meanings emanating from the Brazilian edition, we also focused on the questions concerning its discursive ethos (MAINGUENEAU, 2001; 2006). Finally, S., being a hybrid work of book and puzzle book, is related to the scenography of games and the world of entertainment, with the cinematic narrative constructions, pointing to the functioning of this other matrix crossing the literature, which opens space for the creation of other materials, for the expansion of its universe based on its narrative and materiality, and achieves its objective: to be both a codex and a multiple, accompanied by several inserts, ephemeral, emulating literature.
Efeito da espécie invasora Hedychium coronarium J. König (Zingiberaceae) na evapotranspiração e no lençol freático
(Universidade Federal de São Carlos, 2020-03-06)
Vergne, Driélli de Carvalho
;
Wendland, Edson
;
https://lattes.cnpq.br/3893936996168895
;
Matos, Dalva Maria da Silva
;
https://lattes.cnpq.br/4728734263109138
Biological invasions are among the main causes of biodiversity loss, to being able to change processes at ecosystems’ level, such as primary and secondary productivity, nutrient cycling, hydrological cycle, soil development and disturbance frequency. Changes caused by invasive species in the composition of environment can reduce or eliminate the provision of ecosystem services. Water is one of those services that can be damage by biological invasion, and evapotranspiration (ET) and variation in the water table level can also be affected. We assessed the impact of the Hedychium coronarium species on ET over 14 months and water table level, in an invaded and non-invaded area. We calculated the reference evapotranspiration (ET0) and hence the plant coefficient (Kp) for the invasive species and a group of native species of riparian forest; and we relate the growth of species to the monthly ET. The daily ET exhibited by H. coronarium was low compared to native species and mixture of invasive and native species. Thus, daily ET and accumulated ET of the mixture was higher than the other treatments, showing that the heterogeneity of the vegetation presents higher ET values. In addition, monthly ET was related to height, diameter at ground level and number of genets (for H. coronarium) indicating that species (invasive or native) have greater investment in growth to find light and occupy the area. The Kp values tend to increase over time reflecting the different stages of development of these species. Water table depth was greater in the invaded areas. Our results show that anthropic actions are directly linked to the invasion processes by H. coronarium and that the absence of natural enemies, as well as the competitive ability of this species, favors the environments’ dominance, which reduces evapotranspiration, as well as infiltration of water in the soil. In addition, this species is can cause positive feedback for its own advantage.