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Cistatinas de cana-de-açúcar: produção recombinante em vacúolos de cana-de-açúcar transgênica (e outros sistemas de expressão em plantas) e estudos de interação com uma pseudo protease do coleóptero Sphenophorus levis
(Universidade Federal de São Carlos, 2021-03-29)
Shibao, Priscila Yumi Tanaka
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Silva, Flávio Henrique da
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https://lattes.cnpq.br/1757309852446263
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https://lattes.cnpq.br/1170485433440664
Sphenophorus levis (Curculionidae: Coleoptera) is one of the major pests of sugarcane crop in Brazil and presents cysteine proteases as main digestive enzymes. In this project, we report the characterization of Sl-CathL-CS, a variant of the main digestive cathepsin (Sl-CathL) of the insect S. levis that presents a serine residue instead of a cysteine in the catalytic site at position 138. In addition, a mutant enzyme, in which serine 138 was replaced by a cysteine residue was produced and named Sl-CathL-mutSC. Sl-CathL-CS did not show proteolytic capacity, but Sl-CathL-mutSC was able to hydrolyze milk proteins and the substrate Z-Phe-Arg-AMC (Vmax = 1017.6 ± 135.55 and Km = 10.766 mM). This mutant was inhibited by E-64 (Ki = 38.52 ± 1.2 μM), but not by PMSF, which is an inhibitor of serine proteases, and the static docking showed the difference of the active site of the two proteins. Interaction assay between them and the cystatin CaneCPI-1 revealed that Sl-CathL-CS has greater interaction with this cystatin, indicating that the protein can assist in the insect digestive process by interacting with the plant cystatins thus allowing the true proteases to work. Considering the importance of the cystatin/cysteine proteases interaction, Laboratory of Molecular Biology has dedicated itself to the study of sugarcane cystatins, being responsible for the identification and characterization of six of them (CaneCPI-1 to CaneCPI-6). Among them, CaneCPI-5 presented high inhibitory capability against Sl-CathL, which is the main digestive enzyme of the insect, besides inhibiting other cysteine cathepsins. Interestingly, this cystatin can protect tooth enamel against acid erosion and cavities. For this reason, there is a need to upscale its production to fulfill potential industrial demand. In this work, CaneCPI-5 was produced in a plant-based system by cell lysates (cell-free systems) of Nicotiana tabacum cv Bright Yellow cells (BY-2 cells), transient expression in leaves of Nicotiana benthamiana and stable expression in BY-2 cells. It was possible to obtain 25 μg of purified protein per gram of infiltrated leaf and the protein showed similar inhibition (Ki = 10 nM) to that of CaneCPI-5 produced in bacteria. CaneCPI-5 was also produced in transgenic sugarcane vacuoles and was purified from its juice, yielding 200 ug per mL, which, in the field, would allow the production of up to 15.3 kg of protein per hectare of sugarcane. The transgenic sugarcane events had their resistance against the attack of S. levis larvae assessed, however the transgenic clones failed to express the protein in the juice after propagation in the greenhouse, which may have occurred by gene silencing. This may have been responsible for them not showing increased resistance.
Conhecimento, uso e aceitabilidade do Tratamento Restaurador Atraumático (ART) por cirurgiões-dentistas da atenção básica
(Universidade Federal de São Carlos, 2021-03-29)
Madastavicius, Sheila Cristina Leite
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Aquilante, Aline Guerra
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https://lattes.cnpq.br/5699716799010108
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https://lattes.cnpq.br/1425588129288407
This study is part of the research line “Management of Care, Work and Education in Health” of the Postgraduate Program in Clinical Management at the Federal University of São Carlos (UFSCar), São Carlos-Brazil. Because of the intrinsic relationship between this Professional Masters’ project with health policies, this research investigated the knowledge and opinions of dentists working in Primary Care in the city of São Carlos on Atraumatic Restorative Treatment (ART) and the receptivity of these professionals to use the strategy in the public service. This is an exploratory, observational, cross-sectional, and institutional study, based on a study population of 50 dentists, which corresponds to the total number of professionals working in primary care in the municipal network, 41 of whom answered the questionnaire. The variables herein investigated involved the following: general characteristics of the participants, their knowledge, suggestions, and materials used in the ART technique; intended use and actual use of ART in the public service; and potential barriers of not applying the ART technique, such as: operator’s opinion; operator’s opinion about the patient’s perception; workload; material support; clinical expertise for ART; supervision of the oral health service; oral health assistant. Descriptive analyses were performed on the following data: characterization of the participating professionals, frequency of responses, and comparison with their expected response, as well as associations between the usage and intended use variables and other variables of all blocks using the chi-square test or Fisher's Exact Test (significance value < 0.05). Regarding the general characteristics of the participants, 80.49% graduated before 1995 and 58.54% had more than 25 years of experience in public service. Regarding knowledge of the researched individuals, their suggestions, and the material used in the ART technique, it was noteworthy that 46.34% of the individuals understood it to be a type of provisional intervention or the same as the suitability of the environment; when analyzing the uses and intended application of the ART technique, 90.24% believed this strategy interrupts the process of caries and prevents the loss of the dental element and 70.73% agreed that it should be widely used in Primary Care. Concerning the barriers to not applying the technique, 46.34% of the participants considered it to be an inferior treatment when compared to other restorative techniques and only 39.02% indicated that the manager understands the concept of ART. In view of these findings and given the need for non-invasive procedures in oral health care services due to the arrival of the COVID-19 pandemic, a proposal for ART training for dentists in primary care in São Carlos-SP will be developed in collaboration with the Oral Health Coordination of the city administration.
Determinantes das emissões de gases de efeito estufa da economia brasileira: uma análise de decomposição estrutural (2010 a 2017)
(Universidade Federal de São Carlos, 2021-03-29)
Campos, Carlos Eduardo Espinel
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Castro, Eduardo Rodrigues de
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https://lattes.cnpq.br/5702172673123334
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Oliveira, Maria Aparecida Silva
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https://lattes.cnpq.br/3455553543736709
The evolution of the concentration of different gases in the Earth's atmosphere is a worrying phenomenon, because the ongoing process of global warming is not explained only by natural causes and is largely due to greenhouse gas emissions (GHGs), which are aggravated by human activities, in particular transport-related emissions. Thus, this study aims to assess the structural changes in the Brazilian economy (level of intensity, technology, structure of final demand, volume of final demand, size of the population, among others) related to GHG emissions, from both an aggregate and sectoral point of view and, in this case, focusing on the transport sector, which is responsible for a large volume of GHGs emitted in Brazil. The methodology applied was the structural decomposition analysis (SDA) with an environmental approach, based on input-output matrices estimated for Brazil, between 2010 and 2017 and data from the Sistema de Estimativa de Emissões e Remoções de Gases de Efeito Estufa (SEEG). The results showed that Brazil showed an increase in its level of GHG emissions, fueled both by technological effects and by effects linked to final demand. Therefore, the intensity of emissions and changes in production technologies in economic sectors, combined with population growth and the level of final demand, have contributed to the increase in pollutant emissions in the country. From a sector perspective, the ‘Land Transport’ sector has been characterized as an intense pollutant, as it emits a high level of pollution per monetary unit produced and indirectly influences GHG emissions in other economic activities.
Consequências da exposição intra-uterina ao agonista canabinóide WIN 55,212-2: impacto sobre o sistema cardiorrespiratório e ciclo sono-vigília durante o desenvolvimento em ratos
(Universidade Federal de São Carlos, 2021-03-30)
Patrone, Luis Gustavo Alexandre
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Batalhão, Luciane Helena Gargaglioni
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https://lattes.cnpq.br/5850453468994497
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https://lattes.cnpq.br/8833369074938340
Policies for the flexibility and legalization of Cannabis use in several countries has increased considerably in recent years and the use of drugs of abuse during pregnancy can affect the development of physiological systems including the respiratory control network, mainly due to the susceptibility of the prenatal period to external and pharmacological interventions resulting in possible consequences in the offspring’s postnatal life. Cannabis psychoactive compounds can act directly on the endocannabinoid system present in the Central Nervous System (CNS) already in the early stages of embryonic development, being an important element for structural and functional regulation of CNS maturation, including for the areas responsible for cardiorespiratory control. However, despite the increased consumption of this substance during pregnancy, knowledge about the influence of exogenous cannabinoids on the development of the respiratory system and the consequences on postnatal life is limited. The present study evaluated the possible effects of cannabinoid receptor (CB) agonist exposure during pregnancy on the respiratory control system of neonatal (P0, P6-7 and P12-13), juvenil (P27-28) and adult (P80-81) male and female rats through subcutaneous implantation of osmotic pumps in pregnant female rats at embryonic day 0 and delivered vehicle or agonist (WIN 55212-2, 0.5 mg/kg/day) for 21 days. WIN exposure interfered in a sex-specific maner with breathing regulation of offspring, thereby promoting a greater sensitivity to CO2 in neonatal, juvenile and adult males. An altered chemoreflex in response to hypoxia was observed in P0 and P6-7 newborn males. In females, prenatal treatment resulted only in a hyperventilation during hypercapnia at juvenile age and a reduced ventilatory response to CO2 and O2 at adulthood. In addition, brainstem neuroanatomical analysis showed an increase in the number of catecholaminergic neurons and CB1 receptor expression and alteration of tissue respiration in early stages of males. A significant reduction in lung compliance was also observed in treated juvenile males. Finally, cardiovascular changes were also observed for male and female animals in adulthood due to prenatal WIN exposure. These findings demonstrate that exposure to the cannabinoid receptor agonist during pregnancy results in prolonged and sex-dependent consequences for the cardiorespiratory control system.
Determinação social da saúde e sofrimento psíquico na universidade: uma pesquisa com estudantes do Instituto de Ciências Matemáticas e de Computação (ICMC) do campus da USP de São Carlos
(Universidade Federal de São Carlos, 2021-03-30)
Silva, Emanuela Pap da
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Barbosa Neto, Jair Borges
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https://lattes.cnpq.br/9551566039906499
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Bleicher, Taís
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https://lattes.cnpq.br/9075358493860166
Questions about psychic suffering are often associated with the university environment, and they represent challenges that require institutional policies to be sought. It is crucial to understand how psychic suffering manifests in different university contexts, to know which are its actual dimensions and determinants. Through a critical perspective, knowledge about psychic suffering can allow the interpretation of the health-disease process of organizing social life, work, and social reproduction. This study intends to identify the prevalence of psychic suffering and its associations based on the understanding of social determination in health among undergraduate and graduate students at USP São Carlos, at one of its teaching units, the Institute of Mathematical and Computer Sciences (ICMC). The research was based on an exploratory epidemiological population, observational, cross-sectional, institutional studies with a critical analysis of 310 undergraduate students and 122 graduate students. The data collection was carried out electronically between September and November 2020, through a self-administered questionnaire composed of a socio-demographic inventory and on health issues and the instrumental Self-Reporting Questionnaire (SRQ-20), which signals the presence of psychological suffering by screening for Common Mental Disorders (CMDs). Data was exported to the RStudio program (R CORE TEAM, 2020) and the Stata program version 14 (College Station, Texas, USA) to analyze distributions and associations. Among undergraduate students, the results showed a general prevalence of CMDs was 72.9%. There was an association with family per capita income, income variation from the period before the pandemic caused by COVI-19 and the moment of research, being a cisgender woman, LGBTQIA+ sexual orientation, reporting difficulty in staying at the University, being followed up with a mental health professional and making use of psychiatric medication. In the adjusted analysis, we found an association and odds ratio between positive screening for CMDs and being a cisgender woman (OR = 2.69), reporting difficulties in staying at the University (OR = 3.45) and being monitored by a mental health professional (OR = 3.74). For graduate students, the general prevalence of CMDs was 72.1%. We found an association with family per capita income, reporting difficulty staying at the University, being monitored by a Mental Health professional, and using psychiatric medication. In the adjusted analysis, we found an association and odds ratio between positive screening for CMDs and difficulty staying at the University (OR = 10.3). The prevalence of psychic suffering was high in both groups. These findings can serve as the basis for the development and improvement of public policies for Student Assistance in the context of Collective Health and the Unified Health System. Among the recommendations is the strengthening of the national assistance policy student, with the expansion of coverage to graduate students, strengthening and creating actions and diversity policies that combat prejudice and discrimination at the national and local levels. It is necessary to consider the transformation of the university environment as well as beyond its limits, considering that what is lived at the university is also a reflection of life outside it.
Um modelo de suporte à decisão para seleção e priorização de KPIs baseados nos sete desperdícios do Lean Logistics e no Balanced Scorecard
(Universidade Federal de São Carlos, 2021-03-30)
Santos, José Rodolfo Laurentino dos
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Silva, João Eduardo Azevedo Ramos da
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https://lattes.cnpq.br/3823047207711289
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https://lattes.cnpq.br/9794870424222532
The definition of an efficient set of performance indicators can guide the organizations to reach its main strategics objectives. It is understood as an efficiente set, when the organization have a number of indicators that cover its process but in an adequate amount. In addition, such indicators can be used as a guide for the deployment of all tactical and operational actions that organizations perform. Methodologies based on continuous improvement use indicators for the measurement and control of leaner alternatives in its processes, among which, the logistical processes. In parallel, strategic deployment methodologies such as the Balanced Scorecard (BSC) assist in translating the organizations strategic objectives into their operations. The Lean Logistics is a derivation of lean manufacturing, which focuses on the logistics processes between supply chain agents as well as the internal logistics processes of organization. The focus of lean logistics is to oppose the seven wastes of lean philosophy, applied to logistics processes, as: Overprodutction, Waiting, Transportation, Motion, Defects, Inventory and over-processing. Relating the themes presented, this research proposes a model for the selection and definition of a set of indicadors or KPIs (key performance indicators), necessary for the measurement and control of seven wastes of lean logistics, prioritized by the organization’s strategy, based on the BSC four perspectives. This set of indicators, identified through a literature review, was grouped according to the seven wastes, and used later within a selection method. The use of DEMATEL and AHP decision support tools, helped to choose the main indicators, primarily through the waste in which they were grouped and after ranked considering the BSC’s perspectives as criteria. The research is done through a case study, carried out in a large company, in a moment of the implementation of lean philosophy. As a result, a model proposal was developed to assist the definition and prioritization of indicators, in addition to providing a list of indicators ranked to control the logistics processes, based on the seven wastes of lean logistics and the perspectives of the BSC. These indicators were sufficient to cover the 7 wastes according to the initial grouping. The solution suggested by the methodology considers a final set of seven indicators ranked according to the preference of decision makers in operational and strategic biases within the applied case study, considering the indicators based on financial perspectives as more applicable to support the strategies of the organization.
Quinoa e amaranto: aspectos gerais, hábito de compra e consumo e cinética de hidratação dos grãos
(Universidade Federal de São Carlos, 2021-03-31)
Schmidt, Davi
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Forti, Victor Augusto
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Verruma-Bernardi, Marta Regina
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https://lattes.cnpq.br/5742976162363922
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https://lattes.cnpq.br/3470912236544684
Quinoa (Chenopodium quinoa) and amaranth (Amaranthus spp.) are pseudocereals with elevated nutritional and functional qualities. The first chapter presents a review of the literature on quinoa and amaranth, highlighting their morphological, agronomic, nutritional and socioeconomic aspects. In the second chapter, the objective was to identify the profile of consumers of quinoa and amaranth in addition to observing their consumption habits in relation to these foods. The data was collected through the application of an online questionnaire, which contained questions about the consumer's profile, the knowledge and consumption of quinoa and amaranth, the diversification of food and the preference and choice of packaging. In the third chapter, the objective was to evaluate the hydration kinetics of quinoa and amaranth grains, in order to observe the transfer of water mass to the grains. Tests were carried out at four temperatures (25, 50, 75 and 100 °C), monitoring the mass gain by water absorption and applying the Peleg model to the data obtained. It was concluded that quinoa and amaranth have great potential for composing functional diets, bringing benefits to the health of those who consume them, and that broader dissemination of these foods can contribute to their inclusion in the diet of more and more people.
San Tiago Dantas: ideias e rumos para a Revolução Brasileira (1929-1964)
(Universidade Federal de São Carlos, 2021-03-31)
Ribeiro, Renato Ferreira
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Cepêda, Vera Alves
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https://lattes.cnpq.br/4917331423373631
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https://lattes.cnpq.br/9352262891584264
Francisco Clementino de San Tiago Dantas (1911-1964) was a jurist, politician and intellectual from Rio de Janeiro who played an important role in Brazilian public life between 1929 and 1964. His intellectual production and political action are deeply connected to the construction of the centralized national State in Brazil and to the country’s transition from agrarian to urban, industrial society. Similar to several other intellectuals of his generation, San Tiago Dantas began his trajectory linked to the Brazilian Integralist Action (a fascist inspired movement), converting to the democratic and developmental field after World War II and becoming a leader and an ideologist of the Brazilian Labor Party in the 1950s and 1960s. The objective of this thesis is to investigate Dantas social trajectory - highlighting his networks of relationships and attachment to groups - and his intellectual production - seeking to map his theoretical and political-ideological formulations - in the period from 1929 to 1964. In the research, I used published and unpublished texts by the author (books, speeches, manuscripts, interviews, transcripts of classes and courses), correspondence sent and received, memories and interviews of contemporary characters and newspaper and magazine reports. The analysis of this material allowed the identification of three political-ideological phases in the trajectory of San Tiago Dantas, described in this thesis: 1) catholic/ integralist (1929-1945); 2) non-nationalist developmentalist (1945-1955); 3) laborist/reformist developmentalist (1955-1964).
Avaliação de geradores automáticos de dados de teste com ênfase no teste de mutação
(Universidade Federal de São Carlos, 2021-03-31)
Araujo, Filipe Santos
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Vincenzi, Auri Marcelo Rizzo
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https://lattes.cnpq.br/0611351138131709
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https://lattes.cnpq.br/5213023573055458
Context: With the increasing presence of technology in daily life, it is crucial to guarantee the quality of software products, which can be reached by testing activity. However, testing is highly expensive, once it is responsible for, in most cases, more than half of the software development cost. Among the existing testing criteria, mutation testing is highlighted once it is capable of revealing specific faults in software. Although mutation testing is effective, test data generation and live mutants analysis make the activity unfeasible because it is still required considerable human intervention. Goals: Therefore, this paper aims at evaluating four automatic test data generators (EvoSuite, JTeXpert, Palus, and Randoop), focusing on mutation testing, by measuring adequacy, effectiveness and cost. The first three of these generators implement heuristics during test data generation and they are evaluated both individually and combined, using as baseline a randomic generator. Method: Such generators are compared by conducting an experiment with 33 software products implemented in Java. Adequacy is measured regarding statement coverage, effectiveness regarding mutation score and cost regarding the number of generated test cases. For each generator we generated 10 different test sets for each one of the 33 analysed programs, totaling 1320 distincts test sets. Results: Results show, when we analyze the generators individually, it is not possible to conclude there is a difference between them. However, when we combine the heuristic-based generators, it is possible to conclude there is significant improvement when compared to the randomic generator. Moreover, when we combine all generators, including the randomic one, we obtained improvments of 0,2% in statement coverage and 4% in effectiveness, but we also obtained a significant increase in cost. Conclusion: In general, we observe there is a complementary aspect between the three automatic test data generators and they should be combined to improve the quality of test sets regarding mutation score. However, even with such combination, there is still 25% of the generated mutants which remain alive, indicating there is still room for improvements on quality of the used generators.
Produtividade, qualidade química e sensorial da ora-pro-nóbis fertilizada com adubo orgânico e mineral
(Universidade Federal de São Carlos, 2021-03-31)
Barbosa, Gisiliana de Oliveira
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Fontanetti, Anastácia
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Sala, Fernando César
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https://lattes.cnpq.br/3598362104615834
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https://lattes.cnpq.br/6771975466075202
The search for barbados gooseberry (Pereskia aculeata Mill.) has encouraged its cultivation on a bigger scale. However, the species’ demand for nutrients and response to fertilization is not yet established. The aim of this study was to evaluate the effects of fertilization in agronomical, chemical and sensorial characteristics of Barbados gooseberry in a narrow row spacing and successive crops. The individuals have been cultivated without fertilization, and also with mineral and organic fertilizers, in a randomized block design with 7 replicates. Production analyses were carried out at 210, 270 and 330 days after transplanting (DAT), whereas mineral and protein contents were measured at 210 and 330 DAT and sensory analysis happened at 330 DAT. The mineral fertilization was more efficient to supply nutrients (P,K) and increase productivity.Fertilizers didn’t differ on their leaf protein content, chlorophyll concentrations and sensory characteristics.
Produções de animações em stop motion na aula de física: possibilidades de novas percepções
(Universidade Federal de São Carlos, 2021-03-31)
Morais, Danilo de
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Cruz, Tersio Guilherme de Souza
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https://lattes.cnpq.br/0705292304279277
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Silva, Fernanda Keila Marinho da
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https://lattes.cnpq.br/6390725197649567
In this text I describe the application of the educational product carried out for the preparation of the dissertation of the MNPEF (National Professional Master's Degree in Physics Teaching). The developed product is a text material to guide the production of stop motion animations in the teaching of Physics. The work is based on the concept of perception proposed by Gestalt thinkers. One of the main representatives of Gestalt, Arnheim criticizes the exclusion of intuition in science education as opposed to an overvaluation of inductive logical reasoning. This work is inspired by this criticism to propose a method of visual creation that can be adopted by the teacher as an alternative for visual production that does not depend on computer programming skills typically used in making educational animations and simulators. The creation of stop motion can also be a tool for exercising intuition when produced by students. As for the application in the classroom, both possibilities were tested: video creation by the teacher using concepts of visual perception in the study of movements and the production of videos by students to exercise their perception and also to test the ease of execution of the procedures. guidance material.
Proposta de adaptação de cicloergômetro e os efeitos de sua aplicação no treinamento de membros superiores na capacidade funcional e de exercício e na qualidade de vida em pacientes com DPOC
(Universidade Federal de São Carlos, 2021-03-31)
Gonzaga, Luana Aparecida Vieira
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Jamami, Maurício
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https://lattes.cnpq.br/4274129560250050
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https://lattes.cnpq.br/0722245980119266
Exercise intolerance can affect upper limbs (upper limbs) and lower limbs (lower limbs) differently, with the upper limbs' muscle function being relatively preserved as they are more frequently used in routine activities of daily living (ADL), including personal hygiene or involvement of some of these muscles in breathing work. Despite the relative preservation of the function of the upper limbs muscles, it is common to complain of dyspnea on the part of patients with COPD when performing activities with the limb without support, that is, at or above the shoulders without support. One of the treatments indicated is endurance training, as for example on the arm cycle ergometer. The MMSS training in an arm cycle ergometer allows, by keeping the distal end of the limb supported, that the scapular waist musculature can assist in breathing, prolonging the duration of the exercise and delaying the onset of dyspnea. Despite the benefits such as improved exercise tolerance, quality of life and reduced dyspnea, this method has been passed over to training without support,. Patients with COPD and functional disability may have a greater tolerance for training without support when performing an adaptation period with the upper limbs training on a cycle ergometer, thus regaining their independence. However, there is difficulty regarding its use, since cycle ergometers with the necessary characteristics (measurement of load and cadence, and height adjustment) for the application of training with MMSS support are expensive and generally inaccessible to clinics and patients. Therefore, this study aims to propose a cycle ergometer adapted for upper limbs (article 1) and to verify the effects of its use on upper limbs training of COPD patients (article 2). To adapt the cycle ergometer of article 1, the Mini Bike PRO E13 from Acte Sports® was attached to a table with height adjustment plus a torque wrench on the right crank and a cadence sensor on the table. The study included the participation of patients of both genders, with a clinical and spirometric diagnosis of COPD who underwent an Incremental Exercise Test (IET) on the cycle ergometer adapted until exhaustion. Obtaining as results the evaluation of 13 individuals with COPD, (FEV1 / FVC: 0.50 ± 3.4), 67 ± 3 years, BMI 24 ± 4.14, irregularly active and active. The IET lasted an average of 9 minutes, reaching a maximum load of 4.5 N.m on average, with VO2max reached 0.80 ± 0.25 l/min, equivalent to 36 ± 12% of the predicted. We concluded that the equipment was able to assess the functional capacity when reproducing a submaximal IET in patients with COPD. In article 2, an experimental study was carried out, such as a randomized controlled clinical trial in a controlled block, blind evaluator and parallel group, using the MMSS cycle ergometer adapted in article 1. The training protocol lasted a total of 8 weeks, three times a week, 50 minutes each session. Patients were divided into Intervention (IG) and Control (CG) groups. Both started the sessions with stretching of the main muscle groups, followed by heating for three minutes with no load on the MMSS cycle ergometer at a 70 rpm without load. In the IG, they performed another 30 minutes with a load of 70% of the work rate found in the incremental upper limb test and ended with three minutes of cooling down without load. In the CG, all training on the cycle ergometer was carried out without load. To test the exercise capacity, IET and constant load test (CLT) were performed, to verify the functional capacity, the six minute pegboard and ring test (6-PBRT) and the TGlittre were performed, and to verify the health-related quality of life Saint George Respiratory Questionnaire (SGRQ) is applied. The IG obtained better results after the intervention than the CG in the physical tests IET load (+0.91), IET VO2 (+0.20), CLT time (+ 3min), and only the IG reached the minimum important difference in the CLT (+1.75 min). Both groups reached the minimum important difference in Glittre (GC -38 sec; GI -30 sec). Despite not having found statistical relevance, the present study showed that MMSS training in the proposed adapted cycle ergometer tends to be effective in improving physical capacity and functional status.
Uma leitura midiática sobre o fortalecimento institucional do Ministério Público na Constituinte de 1987/88
(Universidade Federal de São Carlos, 2021-03-31)
Ribeiro, Desirée Ferreira Marques
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Oliveira, Fabiana Luci de
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https://lattes.cnpq.br/9487149052862292
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https://lattes.cnpq.br/4448458537304001
The gradual obtaining of prerogatives from the Federal Prosecution Service started in the dictatorial period and enshrined with the promulgation of the 1988 Constitution, points to a course of institutional strengthening. This development was accompanied by the national press, with articles portraying the protagonists of the debates in this context. This monograph aims to reconstruct this process from the news coverage, identifying actors in dispute, regarding the MPF's prerogatives, during the 1987/88 National Constituent Assembly. The theoretical approach starts from the Bourdieusian concept of the field to identify the narratives and forces in dispute. The methodology is based on document analysis based on three strategies: 1) analysis of news content published by the newspapers “O Estado de S. Paulo” and “Folha de S. Paulo”, between 1985 and 1988; 2) exploratory bibliography study that addresses the lobby carried out by the Federal Prosecution Service; and 3) documentary analysis of the preliminary draft approved by the Subcommittee of the Judiciary and the Federal Prosecution Service, as well as the articles referring to the MPF in the constitutional text promulgated in 1988. The media outlined a positive portrait in defense of an institution's prerogatives and attributions that it was beginning to emerge as a defender of society and that, therefore, constituted a substantial legal protection against the arbitrariness experienced in the regime of exception. There was a congruence between the content of the speeches conveyed by the news coverage, by the MPF, by the preliminary draft of the SPJMP and, in large part, by the constitutional text approved in 1988.
A alfabetização é nossa! Contribuições da educação dialógica à alfabetização e ao letramento do campo: um estudo bibliográfico
(Universidade Federal de São Carlos, 2021-03-31)
Oliveira, Cristiane Fontes de
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Mello, Roseli Rodrigues de
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https://lattes.cnpq.br/5233854244313683
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https://lattes.cnpq.br/8379968467967973
This paper presents a qualitative research in the field of Education, focusing on literacy skills in the modality of rural education, specifically that literacy, as a teaching and learning process, offered to students living in agrarian reform settlements and other peasant communities that practice family agriculture, seeking to verify the contributions of research based on dialogical learning to this theme. This is a bibliographical study, dedicated to the analysis of articles gathered from international scientific indexing databases, with the objective of verifying, analyzing, characterizing, and categorizing the contributions of theories and practices that have, as one of their theoretical foundations, the dialogical learning perspective, especially Paulo Freire's studies on literacy skills in rural education. The research work sought to verify how dialogical education can collaborate to the development of school practices in literacy skills that better correspond to the educational needs of peasant populations. The data were analyzed using the techniques of Content Analysis and Communicative Methodology, with the theoretical input of Paulo Freire and other authors with a communicative base, that is, who take the dialogical perspective as a strategy to overcome inequalities, through critical and problematizing thinking, which is done in the social praxis and constitutes action and reflection in interaction. The research has shown that there is a consensus in the studies of rural education with dialogic foundation about the need to adopt teaching and learning methodologies in literacy skills of rural populations, which take into consideration the rural territorial identity, demonstrating that not paying attention to the specificities can generate exclusion, collaborating to the historical marginalization experienced by rural people. The studies also present a set of didactic and pedagogical indications that point to a specific methodology to literacy skills in rural contexts, in order to value and promote historically subjugated rural identities. It also shows the occurrence of two important subfields of international studies, “Place-based Education” and “Rural Literacies”, which were not found in the national sample set. Thus, this research intended to present contributions to the practice of teaching literacy skills in the modality of rural education, which can promote instrumental learning and critical consciousness and, thus, collaborate to the social sustainability of rural people and their territories, promoting the ethical commitment to help rural communities to think about their demands, problems and, above all, their own social, economic, and cultural survival.
Análise da interação compressor-tubo capilar na adaptação de um sistema de refrigeração de R134a para R290 ou R600
(Universidade Federal de São Carlos, 2021-04-01)
Jesus, Gabriel Fernandes Freitas de
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Ávila, Márcio Turra de
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https://lattes.cnpq.br/8581555057590142
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https://lattes.cnpq.br/6110691088236313
In a context with environmental concern getting more and more importance, products that are aggressive to the environment are receiving less and less demand. For instance, the refrigeration industry is being directed to abandon some not ecological refrigerant fluids (CFCs e HFCs).Just as the R134a has replaced the R22, today the goal is to replace the R134a by more ecological fluids as R600a and R290. In that way, this monograph has as principal objective to analyze the transition of a refrigeration system, which works originally with R134a, to R600a and R290. For that, it was realized a theoretical comparison study, looking for analyzing the impacts that this change causes to the mass flow. It was used a numerical modelings which involved volumetric efficiency for the compressor and the capillary tube length’s calculus. The goal is to achieve the mass flow balance on the system. It was observed that the superheat influence to the capillary tube length is not expressive in comparison to the evaporation temperature. In all the cases, R600a needed a bigger length to the same difference of pressures in comparison to R134a and R290. Besides that, at evaporation temperature equal to -15ºC, an interchangeability is observed between R134a and R290. In that case, it is possible to switch the gas of the circuit with no changes at the capillary tube length. For the R600a, that is not possible at all the studied conditions.
Estudo de percepção das atividades de coordenação e compatibilização de projetos de edificações nos processos tradicionais e nos processos BIM: o caso de São Carlos (SP)
(Universidade Federal de São Carlos, 2021-04-01)
Teixeira, Gabriel Araujo
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Silva, Sandra Regina Mota
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https://lattes.cnpq.br/9973870842834947
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https://lattes.cnpq.br/5451540033439484
Inside the theme of projects management, the present work had the purpose of investigate and analyze building projects’ coordination and compatibilization, starting from data and reflections about procedures shapes applied on these processes. Firstly, as part of the Literature Review, it was made a research into technical and scientific papers, searching for methods and contributions that uses traditional models and modern models based in BIM (Building Information Modeling). The repertoire due this research provided the necessary subsidy to build a practical study, based on quizzes/interviews, using Google Forms, about practices and procedures adopted by architecture and engineer companies that works at São Carlos (SP). This work investigation’s double character, with theoretical and practical bases, tried to compare the different methods to better understand the projects’ current scenery on this region, as evaluate its tendencies, identifying that the transition between project’s methods is occurring, instead of the low rhythm, and the professionals mindset about the themes of project’s coordination and compatibilization, and also the quality of their products, exists and is growing.
Conhecimento metacognitivo e diferentes momentos da escolha: compreendendo o processo da escolha profissional e suas relações com componentes metacognitivos
(Universidade Federal de São Carlos, 2021-04-01)
Foresto, Emanuelle dos Passos
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Schelini, Patrícia Waltz
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https://lattes.cnpq.br/6185333517392859
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https://lattes.cnpq.br/9852211884449955
Professional choice is characterized as a process that concerns the construction of the occupational trajectory of individuals and can occur throughout life, including the transitions that may arise along this trajectory. In choosing, two large groups of information are important: knowledge of internal sources and knowledge of external sources, thus, based on the accumulation and management of awareness of the aspects involved, subjects are able to decide more assertively and reliably. In this sense, cognitive and metacognitive skills will play a prominent role throughout the process, for example in the stages of knowledge, understanding and coordination of all acquired and processed information. The general objective of the study was to investigate the metacognitive knowledge for choice, verifying whether there is a significant difference between the variables when considering three moments: first choice, implementation/crystallization and change in choice. In addition, the specific objectives involved the elaboration of the materials used in data collection and the investigation of influencing aspects in the choice, also considering these three moments. For this, two protocols were developed: the Protocol for Assessment of Aspects Involved in Choice and the Protocol for Assessment of Metacognitive Knowledge for Professional Choice. The sample consisted of 108 participants with a mean age of 22.5 years, distributed into groups according to the moment in which they were found. The composition of the groups was as follows: 29 in the first choice group, 49 in the implementation of choice and 30 in the change in choice group. A significant difference was observed in the scores of the Metacognitive Knowledge Assessment Protocol for Professional Choice between first-choice and implementation of choice groups, which may be related to the degrees of development of these skills, whether due to the influence of age group and/or exposure to environments that influence its use and evolution. In addition, for the three sample groups, the main aspects indicated as most influential in the choice were: identification with professional performance, personal values and course subjects.
"É estudo do meio, não passeio!": a educação ambiental crítica através do vínculo entre escolas e unidades de conservação
(Universidade Federal de São Carlos, 2021-04-01)
Lobo, Lara Bittar
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Mariano, Neusa de Fátima
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https://lattes.cnpq.br/5003743301847104
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https://lattes.cnpq.br/1233497225721187
This paper proposes to discuss the contribution of critical Environmental Education to the perspective of environmental studies carried out in Conservation Units, through the analysis of the Management Plan for the National Forest of Ipanema in concomitance with the laws and educational and environmental guidelines that dialogue about the presence of this educational perspective in formal and non-formal teaching spaces. This discussion took place through the rescue of the man-nature relationship through the concepts “nature”, “ecology” and “environment”, which enable the construction of critical Brazilian environmental thinking, together with the concept of “interdisciplinarity” and “study of the environment”, dialoguing with the dialectical approach to properly consider the histories of human intervention in the environment, where critical AE will converge directly on this dynamic. The discussion ends with the report of the experience of a public school teacher who, by exposing his whole process of articulation between the school and the conservation unit in question, demonstrates that his practice converges directly with the elements brought along job.
Estudo dos condicionantes espaciais para avaliação imobiliária utilizando técnicas de inteligência artificial – São Paulo/SP
(Universidade Federal de São Carlos, 2021-04-01)
Honda, Felipe Pereira
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Melanda, Edson Augusto
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https://lattes.cnpq.br/1554762456965991
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https://lattes.cnpq.br/8554345401299021
The increase in the supply of real estate in recent years has influenced the increase in credit, and, however, has raised sales prices. However, the measurement of real estate values is still a very complex process, as it is influenced by numerous factors, among them physical, geolocation and political-economic. Thus, the objective of this research is to present which variables are relevant in the formation of the value of urban properties using artificial intelligence technique. It was decided to use the decision tree technique for mining and statistical analysis, created from the Randon Forest algorithm. Through mining it was possible to notice that there is a strong correlation between the categories and subcategories analyzed, and, however, the influence they have on the formation of the value of urban properties in the 32 administrative regions of the municipality. Among the factors that had the greatest influence on the value of the properties were the proximity to Shopping Centers, police stations, areas where geological hazards occur, slums, consulates, fire departments and train stations. It was also found that the different environmental aspects, positive and negative, influence the real estate valuation and that the adopted methodology is efficient in the evaluation of large databases, as well as an important tool for decision making and for urban planning.
Análise de chatter no torneamento de aço endurecido utilizando o método de elaboração de diagramas de lóbulos de estabilidade
(Universidade Federal de São Carlos, 2021-04-01)
Fernandes, Matheus Broder
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Antonialli, Armando Ítalo Sette
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https://lattes.cnpq.br/4367459395417045
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https://lattes.cnpq.br/2314535766668767
Chatter is a self-excited phenomenon that affects manufacturing processes, which causes a poor surface quality in final pieces, excessive tool wear, waste of material and uncomfortable noise, causing financial loss and bad operation conditions to the operator. This phenomenon can be found in most traditional metal cutting processes. This study researches the occurrence of chatter in Turning operations for specimen of tool-steel class O ASTM A681-O1 using the development of Stability Lobes Diagram (SLD) in 6 different modellings of a metal Lathe considering 2 and 6 Degrees of Freedom (DOF) and rigid or flexible operation conditions. The investigation was based on adopting parameter obtained on the references and estimate some other to get the SLD in graphic of Depth of Cut alim versus the Lathe spindle speed n, after that, compare the parameters adopted in the four condition of cutting of the reference. It was found that for chatter identification processes through SLD, modelling can cause a serious impact in the results obtained, given that using a poor representative model, considering the Lathe a 2 DOF mass-spring-damper system in which the bed mass is the mass of the system, and the spring and dumping coefficients between the bed and ground are the system coefficients, the system was tagged as stable, being safe for chatter occurring. However, when considering the model as a 2 DOF mass-spring-damper system in which the tool plus tool holder mass is the mass of the system, and the spring and dumping coefficients between the tool plus tool holder and the turret are the system coefficients or considering a 6 DOF model dependent on each other, the system was tagged as unstable, in which the phenomenon might have occurred. Finally, one could be noticed that in orde to get a better surface quality in specimens, the author could use better parameters of cutting, like a higher spindle speed or a lower depth of cut.