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Gestão de projeto do design de um gêmeo digital baseada em requisitos aplicada a uma plataforma de manufatura aditiva, customizada com robô tipo Delta
(Universidade Federal de São Carlos, 2022-11-09) Parreira, Edson da Silva Lamounier; Malavolta, Alexandre Tácito; https://lattes.cnpq.br/8477313173581967; https://lattes.cnpq.br/0849173973045338
In the digitalization era several technology features have become essential in order to increase the productivity of industries and decrease the environmental impact during the product life cycle. This means that engineering, marketing, sales, and other areas that constitute a modern company are driven by data, which accumulates and tends to become more complex with time. Information acquisition and data storage technologies are at an advanced stage in the national industries. However, even though data from process areas such as engineering, manufacturing, processes, and services can be obtained, data integration, management, and implementation in the virtual world are not a focus of research. With the growing demand for individualized products, the digital twin offers an effective solution for modeling, development, and innovation. This work seeks to provide a better product-digital interface for better design execution and product development management, making the life cycle more efficient, integrated, and sustainable. Through the conducted study, a framework was developed for the initiation of engineering projects with the intention of building a digital twin and finally, this same project structure was put to test by means of a case study. The construction of the digital twin of an additive manufacturing platform, customized with a Delta-type robot, was started in the form of an integrated project from the requirements, generating at the end, a digital product, with all information integrated and related to the product objects and engineering project. Therefore, it is stated that the created process structure presents a valid and tested strategy for the initiation of any digital twin project, so that the product can follow the life cycle with all the necessary information for further manufacturing and service development.
Imunoensaio de fluxo lateral para diagnóstico de tuberculose
(Universidade Federal de São Carlos, 2022-11-09) Silva, Vinicius Cauan Mendes da; Pranchevicius, Maria Cristina da Silva; https://lattes.cnpq.br/4772148921558977; Nascimento, Isabella Sampaio do; https://lattes.cnpq.br/5598322661148873; https://lattes.cnpq.br/8257371551653352
Tuberculosis is the leading cause of death among infectious diseases, according to the World Health Organization, and represents a major public health problem. About 1.4 million people died from tuberculosis worldwide in 2019, with about 4,500 cases per year in Brazil since 2010. Early diagnosis of tuberculosis is essential for treatment to be more efficient, avoiding nonspecific therapies that contribute to the emergence of resistant forms of the pathogen. Current diagnostic methods, such as immunoassays and molecular tests, require infrastructure and specialized professionals, compromising diagnosis in low-resource settings. In this sense, new biomarkers have been researched for the development of new diagnostic tools. Among these biomarkers, we have the Hsp65 and CFP-10 proteins, which are found in different samples from tuberculosis patients and are secreted in the early stages of infection. Therefore, the present study presents the development of a paper-based immunosensor for the simultaneous detection of Hsp65 and CFP-10 proteins using a lateral flow system. For this, the biomarkers were immobilized on the nitrocellulose membrane for detection through the competitive model, in a device composed of the sample, detection, and absorption zone. Gold nanoparticles were functionalized with antibodies against the targets to act as detection labels. After optimizing the immunosensor in different experimental parameters, such as sample volume and incubation time, it was possible to build calibration curves for both biomarkers for the concentration range of 2.5 μmol.L-1 and 40 μmol.L-1 . The device presented several advantages over the diagnostic methods already used, such as low-cost, easy-to-use, fast results detectable with the naked eye, providing a promising tool for the diagnosis of tuberculosis at the point of care.
Diversidade e distribuição de ciliados da Família Strombidiidae (Ciliophora) no oceano global
(Universidade Federal de São Carlos, 2022-11-09) Leite, Greyce Kelly Espolau da Silva; Huber, Paula; https://lattes.cnpq.br/2063255762418966; Sarmento, Hugo; https://lattes.cnpq.br/4515469289999439; https://lattes.cnpq.br/0716089460688359
Ciliates dominate microzooplankton and play an essential role in marine food webs. By ingesting flagellates, bacteria and other organisms from pico and nano-plankton, and then being consumed by zooplankton, ciliates are the main pathway of carbon flow that links the microbial loop to the classical trophic chain. However, the diversity and distribution of ciliates in the global ocean is still poorly understood due to the difficulty of observing and classifying these organisms under light microscopy. When fixed, most ciliates are lost or change their morphology, making their identification difficult. Thus, the use of molecular tools represents an opportunity to study the ciliate community in the ocean. Within ciliates, the Strombidiidae family is amongst the most abundant, but there are still few systematic studies at the global scale on the distribution, abundance and phylogeny of this important group in the ocean. This study aims to: 1) evaluate the phylogenetic diversity of Strombidiidae, 2) understand its distribution in the global ocean, 3) determine the ecological niche of the most abundant clades. We used a metabarcoding dataset (region V4 18S rRNA) of 1008 samples from the TARA Oceans and TARA Polar circum-navigation expeditions. We constructed a Strombidiidae reference tree with complete 18S rRNA gene sequences from the literature and inserted the metabarcoding sequences for phylogenetic assignment. Then we related the distribution data of these clades with environmental data to define ecological niches. The phylogeny of Strombidiidae revealed 20 distinct clades, almost all of them widely distributed in most oceanic regions. The most abundant clade (Strombidium_clade2) is cosmopolitan. Furthermore, we obtained 12 environmental clades whose sequences (V4 18S rRNA region) are not identical to those described in the reference tree, and thus, may represent new clades. Niche analysis showed that the different clades of the Strombidiidae family had different distribution patterns in the global ocean, determined by temperature, salinity and oxygen. The Arctic Ocean had greater abundance among the Strombidiidae clades and very characteristic niches were observed for these oceanographic regions. This work represents the first study of the taxonomic and phylogenetic diversity of the Strombidiidae family in the global ocean, thus contributing to the knowledge of the ecology of marine ciliates.