Desenvolvimento de instrumentação eletrônica portátil para medida de pH em campo por meio de sensor óptico à base de filme de Polianilina
Resumen
The pH measurement has an important role in biotechnological processes as enzymatic
reactions, fermentation processes and others. This ways the general objective of this work is
therefore the development of a portable electronic instrumentation for reading in field an
optical pH sensor based on a conductive polymer. The polyaniline, when used as an active
element of the sensor it has useful electro-optical intrinsic properties for the measurement of
pH in the range of 2 to 12 properties. Thus, the chemical synthesis of the sensor has been
reproduced and the spectroscopy in the UV - Vis has been used for its characterization. For
the electronic instrumentation, the colorimetric transducer comprises an "Emitted Ligth
Diode" (LED) type "Red, Green, Blue" (RGB), and a light sensor was proposed to measure
the attenuation caused by the sensor optical positioned between the sender and receiver of the
light beam. The calibration method of the optical assembly was efficient, as the response
curve obtained. For the evaluation of the instrument with the sensor, repeatability and
reproducibility of assays were performed by calculating the respective standard deviations.
The results in terms of precision and accuracy were satisfactory for measuring in the range of
2-12. Thus, a microcontrolled, functional and low cost electronic instrument was developed,
presenting relevant advantages such as no need for a reference sensor and no susceptibility to
electromagnetic interference. Against commercial equipments, the instrument has significant
potential and it presents some possibilities of pH measurements in field applied to
biotechnology, agricultural and others.
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