Complexos de rutênio contendo lapachol e lausona: síntese, caracterização e suas propriedades quimioterapêuticas
Abstract
The present work focuses on the synthesis and characterization of
new ruthenium complexes containing the naphthoquinones lapachol and
lawsone, due to the interesting biological properties exhibited by them.
Complexes of general formula [Ru(L)(dppb)(N-N)]PF6,
trans-[Ru(L)(PPh3)2(N-N)] PF6 and cis-[Ru(PPh3)2(lap)2] where L refers to the
ligands lapachol (lap) or lawsone (lau), N-N corresponds to 2,2'-bipyridine, 4,4'-
dimethyl-2,2'-bipyridine, 4,4'-dimethoxy-2,2'-bipyridine and 1,10-
phenanthroline, dppb = 1,4-bis(diphenylphosphino)butane and PPh3 =
triphenylphosphine. All synthesized compounds were characterized by molar
conductivity, elemental analysis, infrared absorption spectroscopy, UV-visible
absorption spectroscopy, 31P{1H}, 1H and 13C{1H} nuclear magnetic resonance,
cyclic and differential pulse voltammetry, and for some complexes, by X-ray
crystallography. The complexes were evaluated with respect their cytotoxic
activity in tumor lines cell MCF-7 (breast adenocarcinoma) and L929 (normal
mouse fibroblast), which showed very promising results with IC50 values in the
range 0.12 to 1.11 μM. In addition, the complexes proved to be more active than
the reference drug, cisplatin, which has IC50 μM to 13.98 MCF-7 lineage and
20.14 uM to L929. For the purpose of elucidating the possible mechanism of
complex action, studies of interaction with DNA and with bovine serum albumin
(BSA) were performed.