Estudos citogenéticos em algumas espécies de peixes da família loricariidae pertencentes à Bacia do Rio Piracicaba.
Resumen
Willing to develop the number cytogenetically reports on fish species belonged to the
Loricariidae family, the actual work had as the basic aim to feature chromosomically three
specimen of fishes from the basin of the Piracicaba river: Hypostomus albopunctatus;
Liposarcus anisitsi and Corumbataia cuestae. The specimen H. albopunctatus and L.
anisitsi which were previously feature cytogenetically in other basins and these
cytogenetically data are correlated to the data obtained on this work, concerning to the
species C. cuestae, the cytogenetically data obtained puts together the first feature of
chromosome framing of a specie belonging to this king. The samples were exposed to
conventional and differential cytogenetically methodologies, involving bandings-C, Ag-
RON e FISH (hybridization fluorescent in situ) with probe 18S. The specimen H.
albopunctatus and L. anisitsi were collected in Piracicaba river (Piracicaba country, SP),
the H. albopunctatus, showed 2n=74 chromosomes (10M, 20SM, 44ST/A) the parts
organized of nucleolus were seen as diversified, with until 3 chromosome marked by Silver
Nitrate, featuring on this specie multiple RONs, being acrocentric chromosome with
terminal bandings on short arms and of small submetacentric pair with telomeric bandings
on short arms, coinciding to the results obtained by de FISH technique. The preparations to
marking C, made it clear large heterochromatin interstitial arm of a acrocentric
chromosomal pair, on short arm of a submetacentric pair pericentromeric marking of a long
arm of a large metacentric pair beyond short markings in centromeric parts of chromosomal
submetacentric. The samples of L. anisitsi showed 2n=52 chromosome (16M, 28SM, 6ST,
2A), after Silver Nitrate staining, the markings were placed on terminal position of a long
arm by a submetacentric chromosomal pair, featuring a single RON, confirmed by FISH.
The cytogenetical result obtained with two specimen on the actual work when compared to
the same specimen already described belonged to other basins they show a considered
conservation state. Differences were found, however, we might suggest that these specimen
seem to be cytogenetically very preserved when we compare the cytogetical results already
obtained to the species H. albopunctatus and L. anisitsi available in literature. The specie
Corumbataia cuestae, colleted at Lapa river (Itirapina county-SP), showed 2n=54
chromosomes (28M; 20SM; 6ST/A) just to Nucleolus Organized Regions were observed,
placed on the terminal part of short arm from number 2 pair featured as metacentric, and
they were seen as heteromorphic thought the size, sequential analysis of metaphases in
Giemsa/Silver and Giemsa/banding-C, discovered that the secondary constrictions
correspond to RONs. The constitutive heterochromatin pattern is observed clearly
nucleolus chromosomal pair, with large pericentromeric heterochromatic blocks. The
results obtained to offer new subsidy for studies evolution of Loricariídae, to open
perspective for best understanding of correlations between species of Hypostominae and
Hypoptopomatinae.