• português (Brasil)
    • English
    • español
  • English 
    • português (Brasil)
    • English
    • español
  • Login
About
  • Policies
  • Instructions to authors
  • Contact
    • Policies
    • Instructions to authors
    • Contact
View Item 
  •   Home
  • Centro de Ciências Biológicas e da Saúde - CCBS
  • Programas de Pós-Graduação
  • Genética Evolutiva e Biologia Molecular - PPGGEv
  • Teses e dissertações
  • View Item
  •   Home
  • Centro de Ciências Biológicas e da Saúde - CCBS
  • Programas de Pós-Graduação
  • Genética Evolutiva e Biologia Molecular - PPGGEv
  • Teses e dissertações
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsAdvisorTitlesSubjectsCNPq SubjectsGraduate ProgramDocument TypeThis CollectionBy Issue DateAuthorsAdvisorTitlesSubjectsCNPq SubjectsGraduate ProgramDocument Type

My Account

Login

Produção recombinante e caracterização de duas cistatinas de cana-de-açúcar

Thumbnail
View/Open
6416.pdf (7.255Mb)
Date
2014-09-05
Author
Miguel, Mariana Cardoso
Metadata
Show full item record
Abstract
Cystatins are reversible inhibitors of cysteine peptidases. The cystatins found in plants are called phytocystatins, and represent an independent subfamily of the cystatins superfamily. Some studies have reported significant pleiotropic effects for recombinant cystatins expressed in transgenic plants, notably including tolerance phenotypes against attack of herbivorous arthropods and pathogens, and against abiotic and biotic stresses. Besides, the recombinant sugarcane cystatin, CaneCPI-4, showed potential to inhibit development of melanoma cells. Thus, the study and knowledge about phytocystatins, become interesting from agricultural and medicinal point of view. The sugarcane genome project (SUCEST) allowed the identification of about 25 putative cystatins in this plant, which were gathered in 4 groups, by phylogenetic analysis. In this study, we propose a new classification for the putative cystatins found in the SUCEST database. Furthermore, we describe the heterologous expression, purification and characterization of two novel sugarcane cystatins, CaneCPI-5 and CaneCPI-6, which showed different inhibitory activities against human cathepsin B. While protein CaneCPI-6 was not able to inhibit this enzyme efficiently (Ki = 1,83 μM), the protein CaneCPI-5 showed a good inhibitory capacity against the same enzyme (Ki = 6,87 nM). The CaneCPI-5 cystatin was also analyzed against recombinant cathepsin L from the beetle Sphenophorus levis (rSl-CathL), and showed a good inhibitory capacity against this enzyme (Ki = 0,059 nM). Finally, both of proteins, CaneCPI-5 and CaneCPI-6, proved to be thermostable when kept at 100°C for 30 minutes.
URI
https://repositorio.ufscar.br/handle/ufscar/5553
Collections
  • Teses e dissertações

UFSCar
Universidade Federal de São Carlos - UFSCar
Send Feedback

UFSCar

IBICT
 

 


UFSCar
Universidade Federal de São Carlos - UFSCar
Send Feedback

UFSCar

IBICT