Desenvolvimento de plataformas nanométricas híbridas baseadas em polímero/enzima visando aplicações em biossensores
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2016-10-27Autor
Rodrigues, Stanley Endrigo Bilatto
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Foodborne illness cases are common in Brazil and in the world. However, in many cases the microorganism causing the illness cannot properly identified. In addition, traditional analyses usually employed for identifying such microorganisms are often expensive and time consuming. In this context, the development of novel methods capable of detecting such microorganism in a fast way, similarly to a pre-selection method to indicate the positive or negative presence of pathogenic, are highly
pursued. In this work, two systems using a concept of Electronic Tongue were developed. The first system used the Layer-by-Layer (LbL) technique to immobilize lysozyme, trypsin and pepsin enzymes in nanostructured films. In the second system, the immobilization process was combined using the LbL technique and covalent bonding for lysozyme and trypsin enzymes. The trypsin and pepsin can react with bacterial sub-products released in the medium, while the lysozyme can react directly
with the gram-positive bacteria cell wall. All parameters required to obtain stable films
with preserved enzymatic activity were investigated and determined. In the first case,
the detection of Escherichia coli using the Electrical Impedance Spectroscopy yielded
detection of bacteria at 105 UFC (intermediate values). The second electronic tongue
was composed by sensing units formed by lysozyme and trypsin immobilized by
covalent and electrostatic (LbL) bonds, allowing the reaction with either by products
or with the bacterial cell wall. Contamined solutions with the Gram-positive
Staphylococcus aureus, Gram-negative Salmonella bacteria and a mixed solution
with both were analyzed. The second system was able to detect small bacteria
concentrations at 103 CFU. Finally, the solution re-suspension treatment showed to
be beneficial for improving the detection results. The electronic tongue was capable
of distinguishing solutions contaminated with bacteria, showing potential to aid in the
prevention of foodbourne diseases.