Avaliação molecular do fígado de girinos de rã-touro americana (Lithobates catesbeianus) por espectroscopia de infravermelho com transformada de Fourier (FTIR)

Abstract

Fourier Transform Infrared Spectroscopy – FTIR – is an easy-to-perform technique that can be applied to different types of samples, such as: solids, liquids, gases, semi-solids, polymers, biological materials, among others. The present work used the FTIR technique to detect possible molecular alterations in the liver of American bullfrog tadpoles (Lithobates catesbeianus) exposed to water collected from the Itupararanga dam in Votorantim (BV) and its reservoir in Ibiúna (RI); both points belong to the Sorocaba river. In this study, molecular changes were observed in the range of 3200 – 2600 cm-1 of the infrared spectrum for the liver of tadpoles exposed to the collected water. The observed changes may be associated with the presence of metals (Al, Cd, Mn and Zn) in the water. Although alterations have been identified in the spectra of the samples that were exposed to the water collected in relation to the control, complementary analyses to this study need to be carried out so that the exposed is, in fact, confirmed. Therefore, in this work, it was endeavoured to understand the changes observed in the spectra and also to propose the use of this technique as an alternative tool for application in ecotoxicology.