Avaliação de parâmetros e produção de materiais de referência de suplemento mineral para nutrientes e contaminantes inorgânicos

Abstract

This paper was prepared a material candidate for reference material for mineral mixture for dairy cattle. Shape certain analytes Ca, K, Na, P, Cu, Fe, Mn, Mg, Zn and contaminants As, Cd and Pb The material was crushed, sieved until a particle size less than 80μm and packaged. A study was conducted in the best sample preparation through the use of factorial design, planning Doehlert and 4 in a closed system open system experiments. Through a principal component analysis, we chose to sample preparation that was used in 3 mL of HNO3 acid 3mol.L-1 and 1 mL of H2O2 in a closed system. Techniques of absorption and emission spectrometry were evaluated for the determination of analytes in each of the study. Form testing for between-bottle homogeneity and within-bottle homogeneity to check if the sample is homogeneous. Were also carried out tests in the short-term and long-term stability. The values assigned to each analyte were obtained by an interlaboratory collaborative program. In the end, the uncertainties were determined for each stage of preparation of candidate material and obtained expanded uncertainty pair each of the analytes under study.