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Estudo de interação lectina-carboidrato por meio da técnica de microbalança de cristal de quartzo
(Universidade Federal de São Carlos, 2006-10-20) Pedroso, Mariele Mucio; Faria, Ronaldo Censi; https://lattes.cnpq.br/8659496864305621; https://lattes.cnpq.br/9851115714877073
In this work a study of proteins Jacalin and Concanavalin A, belonging to the class of lectins, and the interaction with different carbohydrates was accomplished through Quartz Crystal Microbalance (QCM). The lectins were immobilized by self-assembly monolayer by means three methods. In two of them were used the cystamine and glutaraldehyde and the protein immobilized in a single layer or multilayer. In the third method, a solution of thiols, 11-mercaptoundecanoic acid and 2-mercaptoethanol, in a proportion of 1:10, and the reagents reagents Nethyl- N-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were used to formed a intermediated reactive to binding a protein of interest. Significant differences were not observed among the methods, being opted for the lectins immobilization in a single layer using cystamine and glutaraldehyde due to the simple preparation and low consumption of reagents. The formation of self-assembly monolayer formation was studied using the Electroacoustic Admittance, QCM and a flow injection system coupled to QCM. In all studies a cystamine concentration of 15 mmol. L-1 produced the highest frequency variation and therefore the largest observed mass deposition on the gold surface with good repetitivity. However, it was observed a strong interaction between the free cystamine and to that was binding to the gold surface forming a monolayer, but this interaction was not specific. The flow injection system coupled to the QCM technique was also used to study the interaction between the lectin-carbohydrate and lectin-glicoconjugate. This study allows determining that the variation of the quartz crystal frequency was the same magnitude that observed for a system when using a QCM technique. The Langmuir adsorption isotherm was used to calculate the values of the apparent affinity constant for the lectins with different ligands. The apparent affinity constant was 1.4x105 M-1, 9.4x103 M-1 and 5.6x102 M-1 for Jacalin- Fetuin, Jacalin-Galactose and ConA-Maltose interactions respectively. The determinations of the apparent affinity constants using QCM, demonstrated the viability of the technique in the study of molecular interactions.
Análise genética de características de cresciemento e de reprodução em um rebanho da raça Canchim
(Universidade Federal de São Carlos, 2006-10-20) Mucari, Talita Buttarello; Alencar, Maurício Melo de; https://lattes.cnpq.br/7762361734413347
The objective in this study was to estimate genetic parameters for the traits days to calving (DC), days to first calving (DFC) and gestation length (GL), associated with reproductive performance of females, as well as to evaluate the relationships of DC and DFC with scrotal circumference at 12 months of age (SC12) and body weights at 12 months of age (W12), at calving (WC), at first calving (WFC), at beginning of the breeding season (WBS) and at beginning of the first breeding season (WFBS), and of GL with weight at birth (BW) and W12. Analyses were done using animal models and the derivative free restricted maximum likelihood methodology. Estimates of heritabilities varied from 0.02 to 0.09 for DC, from 0.15 to 0.23 for DFC and from 0.04 to 0.41 for GL, depending on the data set and the model used for each trait. Genetic correlations of DC and DFC with SC12 showed that it is possible to improve these female reproductive traits by selecting for increase in SC12. With respect to W12, selection to increase this trait should not produce significant changes in DC, but may increase DFC. Associations of the other female body weights with DFC and DC indicated that heavier females experience lower reproductive performance. Estimates of genetic correlations of GL with BW and W12 indicated that selection to change GL will result in correlated response in BW with no changes in W12.