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Proposta de indicadores de desempenho de processo e qualidade para unidades produtoras de biodiesel do norte do estado de Mato Grosso
(Universidade Federal de São Carlos, 2011-08-31)
Rodrigues, Sergio Guaraci Prado
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Toledo, José Carlos de
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https://lattes.cnpq.br/9421627326330417
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https://lattes.cnpq.br/4946351112537467
Corporate management has been a major challenge due to the changes of behavior of society as a whole. Much has been discussed in relation to meeting the needs and expectations of customers, models are developed (BSC, Sink and Tuttle, Performance Prism among others) in order to integrate the internal and external perspectives and approaches to organizations in order to facilitate understanding and measurement of an increasingly selective market and increasingly agile changes. Among these concerns is the issue of greenhouse gas emissions, the impact of ozone mainly by the use of fossil fuels (petroleum), besides the risk of its scarcity. Thus, research has been conducted in the area of renewable biofuels, such as ethanol, biodiesel, and other bioenergy sources. In these studies, has been shown to be economically viable, but mostly, the feasibility from the viewpoint of decreasing the rate of air pollution, greenhouse gas emissions by the use of biodiesel added to diesel used for transport. Because the biodiesel production process still developing industries with processes still under implementation, early production and / or stage of process improvement, usually with initial concern in technical procedures, variables of financial order, the early production, the soon return of investment, achieving the goals established in the strategic planning, among others, let aside a major issue: the measurement and evaluation of process performance in terms of quality and productivity. In this respect, the need arises for indicators measuring the performance of processes and products of unit producing biodiesel. Thus this dissertation discusses methods, tools and indicators of process performance, with an emphasis on quality and productivity, from the "needs" of the internal users of the company, as well as existing legal requirements. The study of multihulls, with interviews and observations "in situ" with three units producing biodiesel from the northern region of the state of Mato Grosso, supported by semi-structured questionnaire was used as the research method. From tabulation, analysis and compilation of data, have been proposed measurement indicators appropriate to the reality and needs of managers performance.
Estudo de parâmetros cinéticos do sistema comercial Bac-to-Bac® para produção de proteínas recombinantes
(Universidade Federal de São Carlos, 2011-08-31)
Corrêa, Thaís Portantiolo
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Mendonça, Ronaldo Zucatelli
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https://lattes.cnpq.br/0886073943430823
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https://lattes.cnpq.br/9123699930520301
The expression system of recombinant proteins in insect cells/baculovirus (BEVS) was described and introduced in 1983 and since then has become a powerful tool for the production of recombinant proteins of biotechnological interest. This work studied the kinetics of Bac-to-BacR Baculovirus Expression System (Invitrogen ) used for the production of recombinant proteins, evaluating growth patterns and consumption of nutrients from Sf-9 insect cells infected with this commercial bacmid and determining the effect of viral inoculum. For this, Sf-9 cells grew at 29oC in T flasks containing 5 mL of SF-900 II medium or Schott bottles with working volume of 15 mL and orbital shaking at 90 rpm. UFLAG-286 cells were also tested at 29°C in TC-100 medium supplemented with 10% bovine fetal serum. The baculovirus used were the Autographa californica nuclear polyhedrosis virus (AcMNPV) as a wild baculovirus infection model, the bacmid extracted from DH10Bac bacteria and transposed with the transfer vector empty (pFastBac 1), called Bac sprec and the bacmid recombinant AcNPV with the GUS coding sequence of the gene β- glucuronidase, used as a standard for transposition experiments and transfection. Cells were infected at different times (TOI), on the initial phase, the exponential phase or stationary phase of cell growth, and with different multiplicities of infection (MOI) (0.01, 0.1, 1 and 10). It was observed that the MOI does not influence the viral replication, because even with the low MOI the cell growth was completely inhibited. TOI had already affected the production of total cell proteins, and infection in the early exponential phase was that obtained the highest number of total proteins. The supplementation of the cultivation with Bac sprec with serine and glutamine did not lead to increased viral production. The supernatant of cultures infected with Bac sprec was able to inhibit cell death where there was induction of apoptosis by terbutyl, indicating contain some protein with anti-apoptotic activity. This effect was not observed on wild baculovirus.
Desenvolvimento local com economia solidária: aspectos relativos à atuação de atores envolvidos
(Universidade Federal de São Carlos, 2011-08-31)
Amado, Rafaela Fernandes
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Silva, Ricardo Siloto da
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https://lattes.cnpq.br/5346985934880710
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https://lattes.cnpq.br/6134058510884250
Poder e resistência em Michel Foucault: uma genealogia do acontecimento
(Universidade Federal de São Carlos, 2011-08-31)
Temple, Giovana Carmo
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Fonseca, Thelma Silveira da Mota Lessa da
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https://lattes.cnpq.br/7055557169644792
Cette étude a pour objet fondamental analysé la relation entre la résistance et l exercice du pouvoir d après les études de Michel Foucault. Pour ce fair, nous allons développer cette sujet à partir d une généalogie de l`événement dans lequel nous pouvons analysé les écrits de Foucault en travers la notion stoïcien d événement et de la notion nietzschéenne de généalogie.
O processo de constituição da cadeia do biodiesel no estado de Mato Grosso
(Universidade Federal de São Carlos, 2011-08-31)
Ferreira, Geraldo Alves
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Paulillo, Luiz Fernando de Oriani e
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https://lattes.cnpq.br/9919942357089937
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https://lattes.cnpq.br/1463889626426485
The search for renewable energy sources, clean, puts the state of Mato Grosso in the forefront when it comes to biodiesel production, the institutional environmentcreated from the 70's with the modernization of agriculture in the state, facilitated the development Agroindustrial Complex of Soy - CAIS and consequently the biofuel industry that has great relevance to the state's economy. Thus, this paperaims to show the main process of establishing the chain of biodiesel in the state ofMato Grosso on the assumption that the chain of biodiesel is developed with high interdependence of the agroindustrial complex of soy in Mato Grosso. To collectthe data we used a structured questionnaire and semi structured, the researcher presents the guidelines, but also gave the respondent the option of mentioning other points considered relevant and which were not addressed. The questionnaires were administered personally by the author and some others, sente-mails requesting information for institutional aid in the understanding of the relationships of the actors present in this chain. The results indicate the existence of a strong link in the chain of biodiesel with soybean agro-industrial complex in the state and that the production of alternative raw materials for the replacement of soybeans in the biodiesel production chain, is still in its infancy, there is noproduction sufficient scale to offer a competitive with soybeans.
Rotinas de revisão no processo de geração e adoção de tecnologias na cafeicultura: estudos de casos
(Universidade Federal de São Carlos, 2011-08-31)
Bicalho, Ellenise Elsa Emídio
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Nantes, José Flávio Diniz
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https://lattes.cnpq.br/1900976112613026
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https://lattes.cnpq.br/4244515570556074
The project‟s object was to identify how the generation, diffusion and adoption process of agriculture machinery and equipments occur in the coffee production. In order to achieve this goal, a qualitative approach was chosen through a case study. The sector companies and coffee cooperative members were analyzed. The results showed that the technology innovation and generation process is accomplished, mainly by the company itself and through partnership with suppliers and clients. Research institutes, competitors or other institutions did not participated in the innovation and generation process. The main reason for innovative activity accomplished by the studied companies is the high level of competition in the sector. Another factor considered important was the accomplishment of the consumer‟s necessities and wishes. It was observed that the best way of innovation diffusion take place in the participation of events and fairs. The client‟s properties products testing is another way used in the innovation diffusion. By the farmer‟s side, the diffusion takes place through the direct and personal information trade. Among the coffee growers studied, the technology diffusion takes place, many times, by the cooperative, through conferences offered to them. The adoption process meets several challenges to be overdone, but two factors can be outstanding: The labor cost and sales coffee price. These two factors are crucial for the grower makingdecision.
Sistemas agroflorestais e a construção do conhecimento agroecológico em assentamentos rurais
(Universidade Federal de São Carlos, 2011-08-31)
Nobre, Henderson Gonçalves
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Schlindwein, Marcelo Nivert
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https://lattes.cnpq.br/9690898537150950
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https://lattes.cnpq.br/0039137780834982
The Sepé Tiaraju, the first one settlement in the mode of Sustainable Development Project PDS of São Paulo state, founded in 2004 in the sugarcane region of Ribeirão Preto/SP, has as a proposal being an alternative to the conventional forms of land occupation and management of natural resources adopted in the region. Then a group of farmers along with Embrapa Environment, INCRA/SP and other partners has been trying to consolidate a participative strategy of development and generation of agro-ecological knowledge in the settlement using Agroforestry SAFs. Based on this ongoing process in the Sepe Tiaraju settlement, this study uses methodologies such as Participatory Rural Appraisal, Participatory Action Research and Participant Observation to analyze the strategies of development adopted, and the contribution of the SAFs for the construction of agro-ecological knowledge and the consolidation of sustainable production systems. In summary we can say by this study that the Agroecology and the use of participative methodologies are essential to conduct local development strategies, thereby promoting the construction, validation and appropriation of knowledge by farmers. It also became clear the contribution of the agroforestry to conciliate the environmental conservation with the diversified food production for the families selfconsumption and even surplus for commercialization and income generation.
Relação entre línguas: diferentes modos de dizer o português brasileiro
(Universidade Federal de São Carlos, 2011-08-31)
Silva, Adriana da
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Silva, Soeli Maria Schreiber da
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https://lattes.cnpq.br/8794598988783452
Our work intitled Languages relationship: Brasilian Portuguese, several ways is based on the theory of the Semântica do Acontecimento(Semantics of the Events). Our corpus is formed by interviews maked by students linguistic graduate at the Federal University of São Carlos city , in 2009, during the Linguistic course taught by Prof. Dra. Soeli Maria Schreiber da Silva. In this research, we propose to study the functioning of the Portuguese language, so with the imaginary one language . Our objective was to observe it as the Portuguese -speaking establish a relationship with the colonization story at the utterance time. About our analysis, we observe speakers affected by the symbolic subjects that are located one at a time and place. In this sense, subjects are constituted in the Event enunciation, it is that we analyzed their positions, places that speak and the way they talk, how they speak and how their mean words. Thus the goal of this work is the language relationship where we consider, based on this theoretical foundation, that the senses are formed in the enunciative events, connected to the history, to the social as well as to the politics.
Estratégias, condições e obstáculos para implantação de técnicas mais sustentáveis no manejo da água em assentamentos rurais. Caso: assentamento rural Horto Vergel 12 de outubro , Mogi Mirim-SP
(Universidade Federal de São Carlos, 2011-08-31)
Barboza, Renato Matos de Lopes Torres
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Teixeira, Bernardo Arantes do Nascimento
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https://lattes.cnpq.br/1166854416745527
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https://lattes.cnpq.br/2156186273034545
When we analyze the basic needs of human beings, the quantity and quality of available water determines the life quality of a family or community. This research brings to light the importance of access to water to ensure the basic needs of rural communities based on social inclusion and self-sufficiency. A unique case study, based on Martin (2008), was proposed to study water management in the Rural Settlement Horto Vergel in Mogi Mirim-SP. The proposal aims to bring up the strategies and analyze the conditions and obstacles to implement more sustainable techniques in water management in rural settlements. A diagnosis of the settlement was developed based on a survey of existing documents, interviews with settlers, leaders, agents and Technical Assistance and Rural Extension (Ater) institutions working on site, as well as practical activities for implementation and evaluation of participatory methodologies developed during the research. It was found that sustainability in management and conservation of water in the settlement is directly linked to its historical development (camping, entering the lot, opening the water hole wells, installation of the power grid, semi artesian wells, opening of wells, installation of network distribution system, and creation of the rural district), forms of public investment and organizational models used. The institutions of Ater should systematize the adapted technologies for family agriculture already charted and/or tested related to water management and conservation, and develop instruments that will ensure the continuity and transfer of information between entities and teams. The existence of structural and organizational problems within the settlement, in addition to insufficient financial resources, lack of alternative methods and technologies, and research on water management and conservation by the Ater specialists, have interfered and still complicate the development process of the settlement.
O processo de planejamento da produção utilizando conceitos orçamentários para fins de avaliação de desempenho operacional numa empresa fabricante de bens de capital
(Universidade Federal de São Carlos, 2011-08-31)
Pedrino, Cintia
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Politano, Paulo Rogério
The fast growth of business and the new needs of the globalized market require companies to maximize their results, which are measured by the economic and financial market, throughout performance indicators of business activities. To do so, an organization goes, necessarily, through stages of planning and budget control where every sector shows its contribution. The planning process of the companies is divided into strategic planning, tactical planning and operational planning whose focus is to produce products and services efficiently in order to implement the overall goals and strategies of the company. The integration between the sectors as well as the quality of information becomes vital to make a good plan. For this, the use of systems and information technologies supporting the planning may have an important aspect in the search of competitiveness. The purpose of this work is to use the methodology of Action-research to systematize and automate, throughout a management information system, the production planning based on monetary values, distribution and allocation of the production cost, and to promote a maximization of the results.
Ao sabor das águas acreanas. Etnografia das oficinas de vídeo do projeto Vídeo nas aldeias: Ashaninka e Huni Kuin
(Universidade Federal de São Carlos, 2011-08-31)
Busso, Adriana Fernanda
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Cohn, Clarice
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https://lattes.cnpq.br/1714080901123094
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https://lattes.cnpq.br/3430193753255761
This ethnography presents two moments of learning processes by which latter indigenous filmmakers members of the Pontão de Cultura Vídeo Nas Aldeias experience during their training: Shooting and Editing. The research took place from two field works: following the Indigenous Ashaninka Filmmakers Training Workshop of Amônia river in Acre, which resulted in the movie called UMA ALDEIA CHAMADA APIWTXA and the edition process of the Huni Kuin movie called KENE YUXI, AS VOLTAS DO KENE , in Olinda (PE).
O ensino de análise combinatória no ensino médio por meio de atividades orientadoras em uma escola estadual do interior paulista
(Universidade Federal de São Carlos, 2011-09-01)
Vazquez, Cristiane Maria Roque
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Malagutti, Pedro Luiz Aparecido
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https://lattes.cnpq.br/5462523657624050
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https://lattes.cnpq.br/7759375964594345
This paper has the aim to describe the design, development and application of guiding teaching activities in an area that is usually little explored, the Combinatorics. The study was developed through an intervention that consisted of three activities applied to guiding students in four classes of second grade high school of a state school in São Paulo. The activities were designed with the goal of putting students in a position of action and decision making to facilitate the understanding and the process of knowledge construction and were developed in groups of four or five students. The research classified as naturalist, by the fact that data collection was carried out directly on the site where the problem, is the central issue is to verify whether the teaching of Combinatorics, without the excessive use of formulas, through tutoring activities and use of multiplicative principle, can improve the teaching and understanding of that content. The results were obtained by analyzing the activities addressed by the students who were recorded, by observation, by notes taken by the researchers and also by the evaluation at the end of the research. It was found that the activities were essential for guiding a better performance of students who felt more secure and confident to carry out new activities. These activities represent the final product of this work and it is expected that constitute reference material for teachers who tirelessly seek for new methodologies.
Comportamento multicrítico no modelo de Heisenberg frustrado: transições de fases clássica e quântica
(Universidade Federal de São Carlos, 2011-09-01)
Nunes, Wagner Antonio da Silva
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Souza, José Ricardo de
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https://lattes.cnpq.br/0253265789709463
In this work we study the critical behavior of systems described by the Anysotropic- Heisenberg model of spin 1/2 with competitive interactions (J1-J2 Model) on planes, and interplane nearest-neighbor interactions. We applied Effective-Field techniques for finite clusters with two (EFT2) and four (EFT4) central sites, as well as Linear Spin-Waves Theory (LSWT). We define α=J2/J1, as the frustration parameter, which together with the the anysotropy exchange and the spatial one lambda, we obtain the respective phase diagrams. Classic phase transitions are studied at finite temperatures, as well as the quantum ones at T=0. We observed the presence of two ferromagnetic (antiferromagnetic) states, a quantum paramagnetic, colinear ferromagnetic (CF) and antiferromagnetic (CAF) at T=0 for J1 < 0 and J1 > 0. We characterized the type of transition (continuous or discontinuous) existing in them. For T > 0, the same phases are found, with the exception of the quantum and the normal paramagnetic ones. The CAF a CF states are characterized in three dimensions in the following way: spin lines with the same orientation adjacent to lines in the opposite sense, and spin planes with the same orientation adjacent to spin planes with opposite sense. In the two dimensions these two states are equivalent. We analyze the classical and quantum limits of the model through the phase destruction of PQ in the ferromagnetic and antiferromagnetic case under the influence of anisotropies. We also applied to the frustated model in the plane and the λ interaction between planes the Theory of Linear Spin Waves and compared their results with those found for the Effective Field Theory.
A influência do fósforo na toxicidade de cobre e composição bioquímica de Chlorella vulgaris
(Universidade Federal de São Carlos, 2011-09-02)
Souza, Imyra Maíra Martins de
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Lombardi, Ana Teresa
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https://lattes.cnpq.br/6737850858443813
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https://lattes.cnpq.br/3930565568802402
Metal toxicity to microalgae is dependent on environmental conditions, evolutionary history of the microalgae, and previous exposure of the organism to the specific metal. Also, it is importantly influenced by the physiological condition of the algae in the moment of metal exposure. In this research we investigated several combinations of phosphorus (P) and copper (Cu) concentrations on the biochemical composition and Cu toxicity to Chlorella vulgaris. Due to its known toxicity, copper was considered in its free Cu2+ ions specie. Because microalgae physiology reflects the environmental conditions, but it also stores some of the inorganic nutrients, previous to Cu spike in the cultures, the algae were acclimated to each treatment s specific P concentration to be tested in combination with Cu. We considered cells were acclimated to a specific P concentration after its growth rate had been stabilized for at least four generations, always transferring the cells while in the beginning of the exponential growth phase. Biomass and physiological parameters analyzed were cell number (cell.mL-1), chlorophyll-a concentration, dry weight, lipid classes (Iatroscan TLC/FID), and total cellular proteins and carbohydrates at several combinations of P/Cu. The P concentrations tested were 5.0x10-5, 2.5x10-5, 5.0x10-6 and 1.0x10-6 mol.L-1 and the free Cu2+ ions concentrations ranged within 1x10-10 and 5x10-8 mol.L-1, and were determined through ion selective electrode (ISE). Our results showed that Cu toxicity to C. vulgaris increased at low P. Carbohydrate, protein and lipid productions were in general triggered at low P and high Cu, with some exceptions. TAG was the lipid class most affected by stressing situations. AMPL and PL were the lipid classes with the higher percent composition amog the classes; HC, WE and ST were present in minor amounts even under stressing situations.
Estudos citogenéticos em espécies das tribos Hypostomini e Ancistrini (Loricariidae, Hypostominae)
(Universidade Federal de São Carlos, 2011-09-02)
Rubert, Marceléia
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Moreira Filho, Orlando
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https://lattes.cnpq.br/3927076022470557
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https://lattes.cnpq.br/5065670903691241
Conventional and molecular cytogenetic analysis were performed on 15 species of the subfamily Hypostominae, comprising 12 species of the Hypostomini tribe, genus Hypostomus, and three of the Ancistrini tribe, genera Ancistrus and Hemiancistrus. The chromosome number of 2n=52 was observed for Ancistrus multispinis (14m+14sm+24st-a), A. brevipinnis (14m+14sm+24st-a) and Hemiancistrus punctulatus (14m+26sm+12st-a). The nucleolar organizer regions (Ag-NORs) and 18S rDNA sites are simple and terminal for species, A. brevipinnis, A. multispinis and H. punctulatus. In these species, it can be seen that the NORs are also CMA3+/DAPI-. As for the heterochromatin, H. punctulatus showed a small amount of this chromosome component, while A. multispinis and A. brevipinnis showed a larger amount, with large blocks on the long arm of some st-a pairs. In turn, the different species of Hypostomus showed a variation from 64 to 82 chromosomes, exhibiting differences in the karyotype formula between species. The NORs were multiple for most species analyzed, with different phenotypes observed in the number and position, which was also confirmed by FISH analysis, showing an interindividual and interspecific variability. In all species, the NORs were also CMA3+/DAPI _. The heterochromatin pattern distribution was different in each species, being another important chromosome marker. Cytogenetic, molecular, and morphometry analysis were performed in the species Hypostomus albopunctatus (Piracicaba river, SP) and H. heraldoi (Pirapitinga river, GO), including those morphologically similar samples present in the Mogi Guaçu river (SP). All individuals had 2n=74, whereas H. albopunctatus showed 10m+20sm+44st-a and H. heraldoi 8m+20sm+46st-a. These two karyotype formulae were found in the Mogi Guaçu river population. Distinct Ag-NOR phenotypes were observed among the three populations and confirmed by FISH. Regarding the heterochromatin pattern, H. albopunctatus presented terminal bands and an interstitial block. In H. heraldoi, the heterochromatin was found widely distributed in the terminal and interstitial regions of some st-a chromosomes. In these species, the NORs were also CMA3+/DAPI _. RAPD analysis and sequencing of the cytochrome oxidase subunit I (COI) gene were carried out in these two species, in the Mogi Guaçu population and in three more species, H. regani, H. margaritifer, and H. hermanni, obtaining the same dendrogram pattern. All species could be differentiated by their COI sequences, resulting in a total of 16 haplotypes. These analyses, combined with the morphometric data, showed that H. heraldoi and H. albopunctatus are two valid species and the specimens from the Mogi Guaçu river still retain some characteristics of H. albopunctatus, and were provisionally designated as Hypostomus aff. albopunctatus. Due to the large number of species currently included in the Hypostomus genus, along with both the intra and interspecific variability in morphology and color pattern, there are often problems in the taxonomy of this group, which can be assisted by cytogenetic data. This study provided new chromosomal data for Loricariidae, being a valuable cytotaxonomic tool for the Hypostomini tribe. The joint analysis of different characters, such as cytogenetic, molecular, and morphometric, allowed a more precise characterization of different populations of Hypostomus species, helping to clarify their validity as distinct evolutionary units.
Os Tupi Guarani de Barão de Antonina-SP: migração, território e identidade
(Universidade Federal de São Carlos, 2011-09-02)
Almeida, Lígia Rodrigues de
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Peggion, Edmundo Antonio
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https://lattes.cnpq.br/9849805103860827
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https://lattes.cnpq.br/9263580065240516
This thesis discusses how the Tupi Guarani who live in the district of Barão de Antonima, in the southwest of São Paulo state, conceive their territory in the context of demarcation of indigenous lands. Considering that the area they inhabit is currently undergoing under a process of demarcation. It also reflects how this indians rethink this context of territorial shifts, referring themselves as definers of their territories. And discussing the way how the myth treats about seeking for Lands free from evils. It (re) means locating the place where this land can be found. Not in the east, how the classical biography presents, but in all those places that the ancestors lived in, assuming different ways and contexts. The investigations carried out in the field articulated the bibliography review wish made me believe that if the demarcation of the lands didn't make sense to the Tupi Guarani groups, nowadays it became necessary. It's a way to guarantee inhabitable spaces where those groups would be able to reproduce their life style. This way it's not about the limitation of the movement or the fixation on a delimitated territory, but a way to guarantee that those groups continue to dislocate around a vast territory, circulating around villages and around demarcated indigenous lands.
Utilização dos mecanismos de apoio financeiro à inovação tecnológica por empresas de São Carlos - SP
(Universidade Federal de São Carlos, 2011-09-02)
Bueno, Alexandre
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Torkomian, Ana Lúcia Vitale
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https://lattes.cnpq.br/6407503018881551
Deformação da fase dispersa e degradação de blendas poliméricas em extrusora dupla-rosca aberta e fechada
(Universidade Federal de São Carlos, 2011-09-05)
Zborowski Sobrinho, Leonardo
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Canevarolo Júnior, Sebastião Vicente
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https://lattes.cnpq.br/4153664441338178
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https://lattes.cnpq.br/4744657966626932
In order to obtain flexibility of the processing in a twin-screw extruder and operate it as an internal mixer, the second phase dispersion and deformation and degradation were analyzed during processing in an open and closed twinscrew extruder. Three polymer mixtures with different viscosity ratios were reprocessed up to three times. After each reprocessing, samples were collected to in-line characterization in the extruder. Two characterization methods were applied: analyses of second phase deformation via turbidity and degradation analyzes. During measurements, the die pressure was controlled by a PID controller inserted in a software developed to permit the flexibility. In characterization of second phase deformation two discharge methods were used: ramp and step pressure increase and decrease. The level of droplet deformation was observed with a turbidity sensor during the melt flow through the extruder die. In the polymer mixture with viscosity ratio close to 1, the most intense turbidity variation with the die pressure has been detected due to higher reversible droplet deformation, mainly in polypropylene/polystyrene mixture. With high matrix elasticity, it was observed a hysteresis formation of the droplets due to high relaxation time of the matrix chains and with high viscosity ratio the droplet deformation was lower. In in-line degradation characterization, the die remained closed in all experiments, and three modes were applied: constant screw rotation speed, constant die pressure and oscillatory die pressure. Process parameters shifts were observed during experiments, except during high viscosity mixture processing. Immediately after closed die processing, it was applied a melt flow characterization during the melt discharge at constant die pressure. It was detected branching of polyethylene and chain scission of polypropylene and polystyrene.
Desenvolvimento de um sistema de produção recombinante de uma cistatina em uma linhagem industrial de Saccharomyces cerevisiae
(Universidade Federal de São Carlos, 2011-09-09)
Nakayama, Darlan Gonçalves
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Fuentes, Andrea Soares da Costa
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https://lattes.cnpq.br/7234493834465420
Saccharomyces cerevisiae is the most important microorganism used in alcoholic fermentation process. A strain of Saccharomyces cerevisiae widely used to produce alcohol in Brazil is the PE-2, due to its high fermentation capacity. The goal of this study was to develop an expression system for recombinant proteins using the industrial strain of Saccharomyces cerevisiae, PE-2. The protein chosen to be used as a model for this system was the Cane-CPI-1, an inhibitor of cysteine protease. Initially the construction of plasmids containing CaneCPI-1 gene was performed and yeast cells were transformed with pYADE4-CaneCPI-1 construction. The transformed strain was submitted to expression analyses during batch fermentative process with cell recycle. The recombinant protein was expressed in yeast cells and it was possible to purify the recombinant protein by affinity chromatography on nickel column. This purified protein was immunodetected using a polyclonal anti-CaneCPI-1antibody and monoclonal anti His-Tag antibody, which were also able to detect the CaneCPI-1 in a crude extract from Saccharomyces cerevisiae cells. Assays of inhibitory activity performed with the purified CaneCPI-1 revealed its ability to inhibit the catalytic activity of a cysteine proteinase. This study showed that the use of transformed strain did not affect the fermentation process and that the PE-2 industrial strain of S. cerevisiae can be a viable expression system for recombinant protein production and open perspectives for production of any protein of biotechnological applications during fermentation alcoholic process.
Avaliação de parâmetros e produção de materiais de referência de suplemento mineral para nutrientes e contaminantes inorgânicos
(Universidade Federal de São Carlos, 2011-09-09)
Silva, Patricia Helena Toniolo da
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Nogueira, Ana Rita de Araújo
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https://lattes.cnpq.br/7034773971317045
This paper was prepared a material candidate for reference material for mineral mixture for dairy cattle. Shape certain analytes Ca, K, Na, P, Cu, Fe, Mn, Mg, Zn and contaminants As, Cd and Pb The material was crushed, sieved until a particle size less than 80μm and packaged. A study was conducted in the best sample preparation through the use of factorial design, planning Doehlert and 4 in a closed system open system experiments. Through a principal component analysis, we chose to sample preparation that was used in 3 mL of HNO3 acid 3mol.L-1 and 1 mL of H2O2 in a closed system. Techniques of absorption and emission spectrometry were evaluated for the determination of analytes in each of the study. Form testing for between-bottle homogeneity and within-bottle homogeneity to check if the sample is homogeneous. Were also carried out tests in the short-term and long-term stability. The values assigned to each analyte were obtained by an interlaboratory collaborative program. In the end, the uncertainties were determined for each stage of preparation of candidate material and obtained expanded uncertainty pair each of the analytes under study.