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Sistema automático de supervisão e controle de cultivos de alta densidade celular de E. coli recombinante
(Universidade Federal de São Carlos, 2011-12-22)
Horta, Antonio Carlos Luperni
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Zangirolami, Teresa Cristina
;
https://lattes.cnpq.br/4546701843297248
;
https://lattes.cnpq.br/5923938048634505
High cell density cultivations of recombinant E. coli are a fast and economical way to produce recombinant proteins. Through this bioprocess, products with high added value and pharmaceuticals of great importance such as insulin, human and bovine growth hormone, protein antigens for formulation of vaccines, enzymes, among others, are obtained. However, keeping these cultivations within the desired conditions becomes a major challenge, since some variables such as dissolved oxygen concentration (DOC) and substrate concentration are difficult to control. Therefore, the development and implementation of an automatic monitoring and control tool are key requirements for the performance of high density cultivation. The present work has as main objectives to study feeding strategies for high cell density cultivation of recombinant Escherichia coli and develop a computational tool capable of ensuring the implementation of the chosen strategies, performing the monitoring, control and supervision of the cultivations. Fed batch cultivations were carried out under the supervision of the tool in a 5 L in-house bioreactor, equipped with sensors for temperature, dissolved oxygen, pH, pressure and biomass (sensor that measures the concentration of viable cells based on permittivity measurements), peristaltic pumps and connected to the gas analyzer. The tool was developed with LabView 8.0 and MatLab 6.5, being the acquisition and communication with the different bioreactor accessories via compact Field Point. Twenty two fed-batch cultivations with 5 different clones of E. coli, BL21(D3) expressing the enzyme penicillin G acylase (PGA) as well as antigenic proteins of S. pneumoniae (PspA3, PspA245 and PspA4Pro) and E. rhusiopathiae (SpaA) were performed during the development of the tool and the studies of feeding strategy. Both defined medium (HDF modified) as complex medium (ZYM-5052 modified), usually having glycerol as main carbon source and IPTG or lactose as inducers were used. In all cultivations, samples were collected to quantify the concentration of cells (dry weight method in filter of 0.22 m and optical density at 600 nm), organic acids, glucose, glycerol and lactose (HPLC) as well as protein expression (densitometry and NIPAB method for PGA) and plasmid stability (plating). The tool SUPERSYS_HCDCR (registered as a free software) developed, implemented and validated in the performed cultivations, carries out the basic functions of bioreactor supervision software, such as monitoring and data acquisition of pressure, temperature, pH, DOC, fraction of CO2 and O2 in the outlet gas as well as real-time estimate of the respiratory quotient, the rate of oxygen consumption and CO2 production. However, it also has the following special features, including: i) automatic control of air and oxygen flow according to cellular demand, ii) automatic activation of the feed pump at the end of the batch; iii) automatic control of feeding flow rate as function of the specific growth rate inferred in real time; iv) automatic control of feeding flow rate constrained by the concentration of dissolved oxygen, v) audible alarms indicating failures in the process; vi) failure messages sent via email; vii) automatic control of dissolved oxygen concentration; viii) control of the bioreactor pressure; and ix) control of bath temperature. Regarding the studies of feeding strategies aimed at biomass productivity increase in high cell density cultivations of recombinant E. coli, using the supervision tool developed together with changes in the composition of the synthetic culture medium available in the literature, a cellular concentrations greater than 150 g/L was achieved in less than 24 hours of cultivation, corresponding to a productivity of 9.2 g/Lh. This value, which is higher than the reported in the literature, was obtained without acetate accumulation and allowing high production of recombinant protein.
Desenvolvimento e distribuição de espécies de macrófitas aquáticas em áreas alagadas da represa do Lobo-Broa (UHE Carlos Botelho) em função do estado trófico
(Universidade Federal de São Carlos, 2011-12-22)
Medeiros, Guilherme Ruas
;
Tundisi, José Galizia
;
https://lattes.cnpq.br/5730659239785432
;
https://lattes.cnpq.br/9794950718150922
A comunidade de macrófitas aquáticas se constitui como importante componente em ecossistemas lênticos e lóticos, habitando áreas permanentemente alagadas ou sujeitas a inundações. Desta forma o presente estudo caracterizou as áreas alagadas e as possíveis diferenças nas características físicas e químicas dos sedimentos e da água circundante aos estandes das espécies de macrófitas Eichhornia crassipes, Salvinia auriculata e Mayaca fluviatilis ocorrentes no reservatório do Broa em distintos tributários. Estes vegetais possuem importante papel como fonte de matéria orgânica e dos nutrientes fósforo e nitrogênio contribuindo para uma maior estocagem dos mesmos. A qualidade das amostras de água circundante aos estandes foram influenciadas principalmente pela ação dos ventos ocorridas na amostragem do período seco (agosto de 2010), que contribuíram para o revolvimento da coluna d`água agindo diretamente sobre os estandes. A avaliação do estado trófico do reservatório verificou que os ambientes estudados apresentam diferenças nas concentrações de fósforo na água, onde foi possível identificar uma área alagada hiper-eutrófica próximo ao rio Itaqueri e oligo-mesotrófica nas áreas alagadas formadas pelos Ribeirão do Lobo e Córrego das Perdizes. Contatouse que os teores de fósforo e nitrogênio apresentam uma distribuição desigual entre as espécies e também entre as suas estruturas, sendo as raízes responsáveis pelo armazenamento de maiores concentrações destes nutrientes. The aquatic macrophyte communities constitutes an important component in lentic and lotic ecosystems, inhabiting areas permanently flooded or subject to flooding. Thus the present study characterized the wetlands and possible differences in physical and chemical characteristics of sediments and water to the surrounding stands of macrophytes Eichhornia crassipes, Salvinia auriculata and Mayaca fluviatilis occurring in Broa reservoir in different tributaries. These plants play an important role as a source of organic matter and nutrients phosphorus and nitrogen contributing to greater storage of same. The quality of water samples from the surrounding stands were mainly influenced by the wind occurred in the sample of the dry period (August 2010), which contributed to the turning of water column acting directly on the stands. Assessing the trophic state of the reservoir found that the studied environments having different concentrations of phosphorus in the water, where it was possible to identify a hyper-eutrophic wetland near the Itaqueri river and oligomesotrophic in Ribeirão do Lobo and Cóorego das Perdizes. It was noted that the levels of phosphorus and nitrogen have an unequal distribution among species and also between their structures, the roots being responsible for the storage of higher concentrations of these nutrients
Estudos preliminares de produção de ácido glucônico a partir de sacarose invertida em biorreator airlift
(Universidade Federal de São Carlos, 2011-12-22)
Lopes, Sidney Magalhães
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Badino Júnior, Alberto Colli
;
https://lattes.cnpq.br/4796007900701417
The use of sucrose for the production of industrial inputs with higher added value has a great industrial interest, especially glucose and fructose which present many advantages over sucrose in industrial processes. Gluconic acid and its derived salts can be mentioned as second generation products also having wide industrial applicability. This study aimed to perform a preliminary evaluation of the enzymatic process for simultaneous production of gluconic acid and, consequently, its derived salts and a high-fructose syrup from sucrose inverted using glucose oxidase (GOD) enzyme in a concentric draft tube airlift bioreactor with 2.0 working volume. For this propose, the volumetric oxygen transfer coefficient (kLa), obtained under different aeration conditions using solutions with different inverted sucrose concentrations, was firstly evaluated. The results led to a correlation to estimate kLa during the oxidation of glucose to gluconic acid in an airlift bioreactor as a function of air flow rate and medium viscosity. Experimental trials were carried out in batch at 35 °C and pH 5.5 in order to produce gluconic acid with different initial glucose concentrations (20 and 40 g.L-1) in the presence or absence of fructose, and with two different enzyme concentrations. The consumption of glucose and dissolved oxygen was monitored throughout the trials. The kinetic parameters for the process (Vmax, KS and KO) were obtained by fitting of the kinetic model to the experimental values. The results showed that the presence of fructose did not significantly affect the affinity of GOD for glucose due to the small variation of KS, as well as it did not alter Vmax values for equal concentrations of KO showed that, although the dissolved O2 concentration was small compared to that of glucose in the medium, the oxygen was not the limiting factor of the process. The results, even though preliminary, demonstrate the viability of developing a simultaneous production process for gluconic acid and highfructose syrup from inverted sucrose.
As classes de objeto e a linguística de corpus na construção de equivalências para glossário francês-português : o verbo na cozinha
(Universidade Federal de São Carlos, 2011-12-28)
Odorissio, Renata Maria
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Vale, Oto Araújo
;
https://lattes.cnpq.br/2277403284693571
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https://lattes.cnpq.br/5399341243418266
Notre recherche a pour but la construction des équivalences à partir du modèle de classes d objets de Gaston Gross (1994) pour un prototype de glossaire bilingue de la Culinaire. C est-à-dire que notre proposition première vise à l application d une métodologie tenant en compte l importance de l abordage observationnel en ce qui concerne les équivalences, sans nier l importance des définitions dictionnarisées des langues paires, traditionnellement employée dans la Lexicographie bilingue. Ainsi, notre recherche a comme principes épistémologiques les apports du Lexique-Grammaire (GROSS, M. 1975, 1981, 1994), dont les outils ont été fondamentaux aux études contrastives des prédicats appropriés, ceux qui sont représentatifs du domaine en question ; les paramètres de la Linguistique de Corpus (BEBER SARDINHA, 2004) importants tant à la compilation de corpus qu aux analyses des données obtenues. La Lexicographie (KRIEGER ; FINATTO, 2004) tant que téorie, nous offre certains concepts et, aussi, on y situe notre point de départ pour la reflexion à propos des métodes appliquées à la construction des équivalences bilingues. Le Lexique-Grammaire s appuie sur des mécanismes combinatoires sélectifs pour comprendre la façon par laquelle la phrase devient le locus où les éléments linguistiques se recouvrent de définition et ainsi de stabilité sémantique. Cette nature sélective du prédicat, conçu par l association d un opérateur et des arguments, ne doit pas être négligée au moment où on en arrive à la quête des termes équivalents. La phrase se présente comme l unité linguistique minimale et un outil essentiel pour les analyses de degrés d équivalence. À présent, on considère trois niveaux d équivalence : équivalence totale, équivalente partielle et non-équivalence. À partir du recensement de prédicats apparus en corpus, c est-à-dire, d opérateurs et des arguments agroupés en classes d objets, il est possible de vérifier l identité la non-identité sémantique entre ces prédicats appropriés et puis établir des niveaux d équivalences. Nos analyses contrastives permettent d évaluer les incongruences entre les candidats à équivalent et le(s) mieux choisir, tandis que les fréquences nous servent de paramêtres selon la préférence des termes concourants. Il va de soi que la Culinaire ne se restreint plus aux moeurs des foyers puisque le marché gastronomique représentent des secteurs de poids pour l écomonie des deux pays traités par notre recherche et que nos résultats ne sont qu une dégustation du thème présent.
O uso da técnica cliente oculto como ferramenta de avaliação do atendimento aos usuários de bibliotecas públicas: uma experiência brasileira na graduação de Biblioteconomia
(Universidade Federal de São Carlos, 2012)
Furnival, Ariadne Chloe Mary
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Ouchi, Marcos Teruo
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Pinto, Euzébio Luiz
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https://lattes.cnpq.br/1291482506649810
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https://lattes.cnpq.br/4500649569193100
No contexto de uma síntese de alguns métodos e técnicas de avaliação de serviços encontrados na literatura sobre o assunto, este artigo relata a experiência da aplicação da técnica de cliente oculto, ou mystery shopping, para a avaliação de atendimento ao público numa rede de bibliotecas públicas no interior do estado de São Paulo, Brasil. Detalhase como a técnica foi aplicada e os resultados tabulados são apresentados. Excluindo o grupo de controle, 60% das bibliotecas avaliadas conseguiram uma avaliação de “excelente” para a variável “informar”, que se refere à capacidade do funcionário atendente em resolver as perguntas e as pesquisas dos usuários, resultado esse muito satisfatório. Em relação à forma com a qual os “clientes ocultos” foram atendidos, a avaliação global foi igualmente boa, tendo 50% das bibliotecas alcançado o valor “excelente” para a função “acolhimento”. A aplicação da técnica se deu no contexto da oferta da disciplina de “Serviço de Referência e Informação” no curso de graduação em Biblioteconomia e Ciência da Informação de uma universidade federal brasileira, com os alunos da supracitada disciplina agindo como “clientes ocultos” dos serviços prestados aos usuários nas bibliotecas públicas avaliadas. Nesse sentido, espera-se que o exercício contribua para a formação de profissionais bibliotecários reflexivos que possam futuramente propor ações baseadas em evidências.
Análise eletromiográfica da escrita manual: estudo de dois padrões de preensão
(Universidade Federal de São Carlos, 2012-01-05)
Almeida, Pedro Henrique Tavares Queiroz de
;
Vergotti, Iracema Serrat
;
https://lattes.cnpq.br/1833552250793623
Handwriting is an essencial skill for the performance of activities of daily life related to the expression of information in academic, work-related and leisure situations. Among adult population, there is a prevalence of two grasp forms used for handwriting, classified in neurophysiological development as mature or transitional grasps. These grasp patterns may be related to upper limb muscular and postural dysfunctions due to the use of different muscular groups in handwriting activity. The objective of this study was to analyze the electromyographic activity of upper trapezium, biceps braquii, extensor carpi radialis brevis and flexor digitorum superficialis among subjects who use the dynamic tripod grasp and the static tripod grasp during handwriting. Twenty-four university students between 18 and 28 years, without functional dysfunctions of the upper limbs underwent electromyographic analyze during a patterned handwriting task. A significant increase in electromyographic activity of upper trapezium and biceps braquii was observed among subjects who use the static tripod grasp. There was no significant change between the myoelectric activity of the extensorcarpi radialis brevis and flexor digitorum superficialis between the two groups. The increase in electromyographic activity among subjects using static tripod grasp indicates potential higher energy expenditure and increased risk of muscular lesions with the maintence of this motor pattern during handwriting tasks. Since the increased demand for speed and expression of information through handwriting is expected during academic life, the careful observation, evaluation and therapeutic monitoring could stimulate the development of mature grasps and optimize the performance of writing tasks, an important area of intervention and clinical research to occupational therapy.
Algumas extensões da distribuição Birnbaum-Saunders: uma abordagem bayesiana
(Universidade Federal de São Carlos, 2012-01-09)
Cahui, Edwin Chaiña
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Cancho, Vicente Garibay
;
https://lattes.cnpq.br/3503233632044163
The Birnbaum-Saunders Distribution is based on an physical damage that produces the cumulative fatigue materials, This fatigue was identified as an important cause of failure in engineering structures. Recently, this model has been applied in other areas such as health sciences, environmental measures, forestry, demographic, financial, among others. Due to it s importance several distributions have been proposed to describe the behavior of fatigue resistance. However there is not an argument about which is more effective for the analysis of data from fatigue. A major problem to choose a statistical distribution, is that often several models fit the data well in the central, but, however, the extremes of distribution raise questions about the decision to select some of their models. The lack of data at the extremes distribution is justified to consider other arguments like the use of a specific statistical distribution, and thus reject other models. In this work we study some extensions of the distribution Birnbaum-Saunders with a mixture of normal scale, in which the procedure will for obtaining inferences will be considered from a Bayesian perspective based on the methods Monte Carlo Markov Chain (MCMC). to detect possible observations influential in the models considered, we used the Bayesian method of analysis influence in each case based on the Kullback-Leibler divergence. Moreover, the geometric Birnbaum-Saunders model is proposed , for data survival.
Processo de trabalho e produção do cuidado de enfermagem em uma instituição de apoio ao indígena
(Universidade Federal de São Carlos, 2012-01-09)
Ribeiro, Aridiane Alves
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Arantes, Cássia Irene Spinelli
;
https://lattes.cnpq.br/4246837549578145
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https://lattes.cnpq.br/8256619308165279
This study aimed primarily to examine the workers conceptions about the process of nursing care in an institution of attention to indigenous health care. The starting point was the reference of the work process and its relation to the use of technologies in health care production. It was also considered the National Policy for the Health of Indigenous Peoples, particularly the distincted attention that should permeate the work process at the institution being studied. The empirical material was obtained from document analysis, systematic observations, guided by the flowchart Merhy Analyzer and by ten interviews with the nursing staff. A thematic analysis of categorical data was conducted and it revealed a fragmented labor process, focused on procedures and cast by institutional and bureaucratic rules. In the process of nursing work there is a clear gap from thinking to acting because planning the Schedule is a job for the responsable worker and the nursing station performs it. The worker s reports showed a sense of equality among human beings in the caring process. Therefore, it was also learned that in some situations, there is an establishment of asymetric relationship showing a certain denial of what is said. The assisted Indians in Support House are perceived by respondents as a retracted and suspicious individual. The care process should be based on a relationship based on trust. To approach the Amerindian, the nursing worker needs to undertake, especially light Technologies, empathy and affection. However, work in live action, hence the use of relational technologies, can be captured by the rigidity of the bureaucratic organizational structure. The act is cast and creative potential of workers is not valued. The caring process presents a special care, the monitoring, which is the activity of nursing staff to accompany the Indians to specialized health services outside the home support. Although the National Policy advocates a distincted attention, the work process in the institution being studied, seemed not to potentiate nor favors a care where it is considered the indigenous peculiarities of self-health care.
Bases conceituais da teoria histórico-cultural : implicações nas práticas pedagógicas
(Universidade Federal de São Carlos, 2012-01-10)
Garay González, Abel Gustavo
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Mello, Maria Aparecida
;
https://lattes.cnpq.br/8338726751746542
;
https://lattes.cnpq.br/3053243876333008
The intention of this research was to analyze and clarify the conceptual foundations of the Historical-Cultural Theory, based on the Historical-Dialectical Materialism by Marx, and their direct implications for the pedagogical practices, specifically on the processes of teaching and learning, in order to contribute to the Brazilian Basic Education, in the search to understand and overcome the problems related to the difficulties of teaching and student learning. The Historical-Cultural Theory is based on the Historical-Dialectical Materialism by Marx and has its methodological foundation founded in the microgenetic analysis. The Marxist theoretical presumptions assists Vigotsky s analysis with the discussion of humans being not only a biological structure, but rather a consequence of the historical-cultural relation, having work as the main mediator. Vigotsky and collaborators present important theoretical and practical contributions to the understanding of the dialectical relations between Teaching and Learning. Vigotsky s microgenetic analysis shows us how to overcome this dilemma between the processes of teaching and learning. From Vigotsky s point of view, the pedagogical processes are intentional, deliberate, and the school is the place where the intentional pedagogical intervention triggers the learning and quality teaching processes. The research is of a bibliographic nature and uses two sources: the primary ones, pieces of work of authors from the Historical-Cultural Theory, and the secondary ones, pieces of work of authors who interpret the primary pieces of work. The question that guided the research was: What are the core concepts of the Historical-Cultural Theory, based on the Marxist theory and its implications for the pedagogical practices? The objectives outlined to answer this theme were: to analyze, in the writings of the Marxist philosophy, the major theoretical points of the Historical-Dialectical Materialism that assist the Historical-Cultural Theory; to clarify the contributions to the Historical- Cultural Theory to the understanding of the dialectical relations in the processes developed in the pedagogical practices. The results clearly show the necessity of overcoming the biological paradigm of Education and presenting an educative model oriented around the viewpoint of society, of human being and of knowledge as highly social, as well as the conceptions of teaching and learning as processes of human activity mediated by human relations and of nature itself. An educative model oriented around the teacher s mediated activity in students zone of proximal development. The work intended to contribute to the advancement of pedagogical practices and in-depth academic discussion about the mediated activity and the zone of proximal development, as focuses of teachers performance, towards school teaching and learning problems.
Comprometimento funcional tardio do membro superior e qualidade de vida de mulheres submetidas à cirurgia do câncer de mama
(Universidade Federal de São Carlos, 2012-01-12)
Assis, Márcia Regina de
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Vergotti, Iracema Serrat
;
https://lattes.cnpq.br/4710357803442871
Breast cancer is the most common malignancy among women in Brazil. In recent years there has been significant progress involving surgical techniques, and a corresponding increase in the number of breast-conserving surgical procedures. However, in terms of function complications and pain, post-operative morbidities still represent a clinical problem. These sequelae and complications have a strong impact on the daily lives, occupational performance and the quality of life of breast cancer patients. The objective of this study was to verify if there is a relationship between late upper limb complications and health-related quality of life in women who have undergone breast cancer surgery. Eighty-one women who had breast surgery between 1 and 5 years previous participated in the study. It was also carried out a survey of the complaints brought by patients regarding the upper limb and the application of the Disabilities of the Arm, Shoulder, and Hand (DASH) and the European Organization for Research and Treatment of Cancer (EORTC QLQC-30 and BR23) questionnaires. The main complaints reported by the patients were as follows: numbness (67.9%); muscle weakness (49.4%); pain (45.7%); lymphedema (22.2%); neck pain (9.9%); and range of motion limitation (6.2%). There was a statistically significant correlation between the morbidity reported by patients with upper limb dysfunction assessed using the DASH and the quality of life related to health. It was concluded that late function complications in women who have undergone breast cancer surgery present a significant impact on the upper limb, in daily life and the health-related quality of life. To invest in occupational therapy can improve functionality not only the upper limb, but also the future perspective, social and emotional function.
Efeitos de carboidratos no desempenho, na digestão e no metabolismo de matrinxã (Brycon amazonicus) e híbridos de pintado (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum) em um período curto de alimentação
(Universidade Federal de São Carlos, 2012-01-13)
Camilo, Rodrigo Yamakami
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Moraes, Gilberto
;
https://lattes.cnpq.br/4427903557163246
;
https://lattes.cnpq.br/6399985363412772
Improvements in fish farming production require continuous research in nutrient formulation and diet technology. Feeding is the most important handling daily practiced by fish farmers and diets must supply the nutritional requirements to increase fish biomass. Aimed at improving the diets, a study was conducted to determine the ability of matrinxã (Brycon amazonicus) and of hybrid pintado (Pseudoplatistoma corruscans X Pseudoplatystoma fasciatum) to utilize different sources of carbohydrate in their diets. Triplicate groups of juveniles matrinxã were fed visual satiety one of five diets isonitrogenous (30% crude protein) and isolipidic (10% crude lipids) containing: 40% glucose, 40% maltose, 40% dextrin, 40% pregelatinized or 40% raw starch for 27 days. Weight gain, feed conversion ratio, feed consumption, digestive enzymes (amylase, maltase, unspecific proteases, trypsin, chymotrypsin, unspecific lipase and alkaline phosphatase), metabolites (glucose, lactate, pyruvate, ammonia, protein, fat acids, triglycerides, amino acids and glycogen) were measured. The same was done with juvenile hybrid, but the diet containing 30% crude protein, 10% crude lipids and 30% one of five carbohydrates. The matrinxã amylase and maltase activities were higher than those of pintado. In both species, fish fed diet containing glucose had the worst weight gain, feed conversion ratio and feed consumption, digestive enzymes (P < 0.05) among all groups. These results were followed by fish fed with diet containing maltose. Weight gain and feed conversion of matrinxã fed with diets containing pregelatinized starch or raw starch and of hybrid pintado fed with diet containing pregelatinized were higher than those of fish fed with the other carbohydrates. In intestinal of both species, amylase activity was higher in group fed with raw starch diets. In matrinxã, the activities of other digestive enzymes showed no significant changes. In pintado the highest maltase activity was observed in fish fed with pregelatinized. The alterations in plasmatic, muscular and hepatic metabolic intermediaries concentration become evident that the matrinxã and hybrid pintado were able to adapt its intermediary metabolism to the changes on the feeding condition to preserve vital functions.
Modelagem de eventos raros: um estudo comparativo
(Universidade Federal de São Carlos, 2012-01-16)
Scacabarozi, Fernanda Nanci
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Diniz, Carlos Alberto Ribeiro
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https://lattes.cnpq.br/3277371897783194
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https://lattes.cnpq.br/3609264817897147
In some situations, in various areas of knowledge, the response variable of interest has dichotomous distribution extremely unbalanced. In the _nancial market is the common interest in determining the probability that each customer will commit a fraudulent action, and the proportion of customers fraudsters is extremely small. In health there is interest in determining the probability that a particular person will present some epidemiological infection that a_ects only a small fraction of the population. However, there are studies that show that the usual logistic regression model, widely used in the modeling of binary data, does not produce good results when it is built using databases extremely unbalanced. In the literature, we _nd some proposals for adjusting models them that take into account this characteristic, such as KZ estimators suggested by King and Zeng (2001) for the logistic regression model applied to databases with events rare. We present this methodology and a simulation study to verify the quality of these estimators. Other proposals in the literature are limited logit model suggested by Cramer (2004) that upper limit to the probability of success and the generalized logit model suggested by Stukel (1988) which has two shape parameters and works better than the usual logit model in situations that the probability curve is not symmetrical around the point 1 2 . In this paper we present some simulations to verify the advantages of the use of these models. Palavras-chave: model logit model limited, generalized logit model, logit model with response of origin, KZ estimators, measures forecasts.
Aspectos da biologia, caracterização foliar e manejo químico de Conyza bonariensis (L.) Cronquist (Asteraceae) e Conyza canadensis (L.) Cronquist (Asteraceae)
(Universidade Federal de São Carlos, 2012-01-16)
Inacio, Estela Maris
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Monquero, Patricia Andrea
;
https://lattes.cnpq.br/2766526793830455
;
https://lattes.cnpq.br/5689412549407138
The genus Conyza includes approximately 60 species, which are distributed throughout most of the world. Globally the weed species Conyza canadensis and Conyza bonariensis infest more than 40 types of crops. Both species are noted for their ability to infest abandoned areas (vacant lots and roadsides), pastures, perennial crops (citrus and coffee) and annual crops (cotton, corn, soybeans and wheat). Knowledge of the biology of these weeds is required to inform management actions to control their spread for example to identify which is the best control strategy and time for action. We conducted experiments at the Center for Agricultural Sciences / UFSCar, between 2010 and 2011, to achieve 3 research objectives: (1) determine the germination behavior of C. canadensis and C. bonariensis under different environmental conditions [different temperatures (10oC, 15oC, 20oC, 25oC, 30oC and 35oC), light (with and without), sowing depth (0, 0.5, 1, 2, 3, 5, 7, and 10 cm) and germination in soils with different textures (sand, intermediate and clay)], (2) characterize the different types of leaf surface of C. bonariensis and C. canadensis by scanning electron microscopy, (3) determine the effects of the application of the herbicide glyphosate (0.42 kg a.i. ha-1), when mixed with ammonium glyphosate (0.5 kg a.i. ha-1), bentazon (0.72 kg a.i. ha-1), chlorimuron-ethyl (0.15 kg a.i. ha-1), carfentrazone-ethyl (0.03 kg a.i. ha-1), 2,4-D (1.0 L a.i. ha-1), metribuzin (0.48 kg a.i. ha-1) and sulfentrazone (0.6 kg ha-1), in addition to these herbicides applied in isolation. For both C. bonariensis and C. canadensis, the best seed germination and germination rate temperature was approximately 250C. Seed germination rate of both species was higher when exposed to light, compared with seeds germinated in the dark. Sowing depth and soil texture also influenced germination, with seeds of both species showing higher emergence rates in intermediate texture soils when they were sown on the soil surface (0 cm), whereas emergence rates declined at sowing depths ≥0.5 cm. Analysis of leaf surfaces revealed that both species are amphistomatic with stomata on the adaxial and abaxial leaf surfaces, that the non-glandular trichomes are unicellular and multicellular, and that C. bonariensis has a visually larger amount of trichomes on the adaxial surface. The most effective herbicide treatments for the chemical control of C. bonariensis were glyphosate + ammonium glyphosate, glyphosate + bentazon, glyphosate + chlorimuron - ethyl and glyphosate + metribuzin, whereas for C. canadensis, the best treatments were mixtures of glyphosate + ammonium glyphosate, glyphosate + bentazon and glyphosate + metribuzin.
A cultura como mediadora do desenvolvimento das funções psíquicas superiores na criança: a atividade docente de musicalização na Educação Infantil
(Universidade Federal de São Carlos, 2012-01-23)
Souza, Carlos Eduardo de
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Mello, Maria Aparecida
;
https://lattes.cnpq.br/8338726751746542
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https://lattes.cnpq.br/0180561132480429
The present work discusses culture as promoting the development of humanity in humans. The concept of culture working is based on the marxist concept that comprises with culture all production developed by humankind throughout its history. The Historic-Cultural Perspective developed by Vigotsky, based on the theoretical assumptions of dialectical and historical materialism of Marx, was used as a theoretical framework for understanding how culture transforms the humans, modifying its natural functions in higher mental functions. In the methodology we seek to draw a relationship between the historical-cultural theory and its grounding marxist, demonstrating how Vigotsky use of dialectical materialist method in carrying out his studies on the human psyche. In the methodological procedures is presented the form of data collection, which characterizes this research of bibliographic nature, showing what were the criteria for selection of theses and dissertation that were analyzed. In data analysis we seek to demonstrate through the performance of the teacher in the process of teaching music, as he in his mediating activity can promote the chidren s access the culture and, how he, making his practice in the zone of proximal development can enhance the development in of higher mental functions. We discussed how the child in their activity mediated by intencional social relations (in this case, the musicalization at school) and access to culture, develops higher mental functions, unique to humans. Keywords:
Mapa de caracterização do texto científico : produção e funcionamento em disciplinas de química do ensino superior
(Universidade Federal de São Carlos, 2012-01-24)
Oliveira, Jane Raquel Silva de
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Queiroz, Salete Linhares
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https://lattes.cnpq.br/7925870606416937
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https://lattes.cnpq.br/2800602875373171
The main objectives of this research were to develop and to implement a tool ‐ the Characterization Map of Scientific Text ‐ which could assist students in recognizing the structural and rhetorical aspects of the scientific language and to investigate its functioning as a facilitator in improving scientific writing of undergraduate chemistry students and as a scientific texts analysis tool. We adopted the theoretical studies of Latour, Coracini, and Campanario on the rhetoric aspects of scientific articles and the studies of Oliveira and Queiroz on the structural aspects of scientific texts in the chemistry field. We analyzed the main characteristics of the scientific language and elaborated the categories of analysis of the scientific text structural and rhetorical elements, as well as the Preliminary Characterization Map of Scientific Text. We also developed didactic materials about the structural and rhetorical aspects of scientific texts, which were applied in higher education chemistry courses. We analyzed the written production of students in activities about rhetorical aspects using peer review. We analyzed the final scientific texts (reports and miniarticles) written by the students and scientific articles written by researchers in the chemistry field. We also investigated the criteria used by professors in the evaluation of scientific texts assigned related to the courses. From these results we developed the Final Characterization Map of Scientific Text, including considerations reported in the studies of Kelly and Takao, Zohar and Nemet, and Sandoval and Millwood. We also developed didactic material about this final Map, which was applied in a different class. We analyzed the initial and final texts produced by the students of this class, as well as their responses to an evaluation questionnaire about the activities based on the Map under consideration. In the activities about the rhetorical aspects, the students developed skills to recognize and analyze critically such strategies in the scientific texts. In the peer review activities, although some rhetorical aspects were also mentioned, the students focused on the structural aspects of the scientific texts. The texts written by the students showed few rhetorical resources compared to those written by researchers. Although considering some rhetorical aspects, the professors focused on structural aspects in the evaluation of scientific texts assigned related to the course. From the activities based on the Final Characterization Map of Scientific Text, the students understood how the scientific text is structured and strengthened, and they improved their scientific writing using structural and rhetorical elements adequately in written texts.
Família do doente com câncer: percepção de apoio social
(Universidade Federal de São Carlos, 2012-01-24)
Rodrigues, Juliana Stoppa Menezes
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Ferreira, Noeli Marchioro Liston Andrade
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https://lattes.cnpq.br/6899526646546927
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https://lattes.cnpq.br/3778200455180493
Cancer is a chronic degenerative disease, considered a public health problem and one of the consequences is to induce the family to live a long time with a diseased family member. This can brake the family apart, because of the treatment process and also causing a demand for a social network that offers the support needed to cope with this complex trajectory. This study is quantitative, descriptive, exploratory cross-sectional, which aimed to characterize the feeling from the families about the social support in cancer patients, assisted by a specialized service in a city of Sao Paulo. All researches considerations with humans were respected. Interviews were conducted with every family that met the inclusion criteria, comprising 69 families and 161 people total. It was used for data collection the following instruments: The family profile of the patient with cancer , Social Support Scale of the Medical Outcomes Study (MOS-SSS) and The Convoy of Social Support Diagram. The interviews were done at their homes with at least family dyads and also after the consent and respecting the date/time stipulated by the family. Data were coded and entered in Microsoft Excel and Biostat 5.0 and analyzed using descriptive and correlational statistics. Patient profiles showed: 65% were female, aged 28-89 years-old and the most prevalent neoplasm was breast cancer (36%), of which 48% were in advanced stages of disease. The second most frequent cancer was prostatic (10%), of which 72% were older 65 years-old. The Convoy of Social Support Diagram showed 506 members in the patient social networks, between 1-89 years-old. The most social network members was female, who knew the patient for 33 years-old on average and 78.7% lived less than 30 minutes from the patient house. The profiles of families showed 28% with only two people in their composition, living in social class D (70%). The MOS-SSS identified that affective support was the most frequently mentioned (88), followed by emotional support (81), material support (80), information support (78) and positive social interaction (76). An average positive correlation was found (p=0.002 e r=0.4) between social support and number of people on the network. Statistically significant associations were found with p<0.0001 between the dimensions of social support and p<0.0003 between the variables number of women in social networks and social support, indicating that social support tends to be greater in larger social network and especially if people are female. It is considered that the possibilities for support of the family are diverse and health professionals have an important role in increasing the viability of the contacts between the micro system (family) and supra system (neighborhood, community and religious organizations), but they must be open to the family, realizing it like a unit of care, recognizing the social network as a fundamental source in the maintenance of wellbeing which improves life satisfaction and effective support. So, identifying and giving the social network its value in the family context and understanding the real needs and potential of the family unit, helps the health professional in the planning of the care centered in the family. This may improve the confront of the families face difficulties inherent to chronic situation and provides a humanized care. The implement of public policies which promote access of population to social support networks is very important.
Caracterização da fabricação e uso de compósitos poliméricos contendo fibras naturais com ênfase no polipropileno/sisal a partir da análise de documentos de patentes
(Universidade Federal de São Carlos, 2012-01-24)
Scopel, Felipe
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Gregolin, José Angelo Rodrigues
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https://lattes.cnpq.br/7842651773105125
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https://lattes.cnpq.br/3716210326293016
Natural fibers have became important as substitute for synthetic fiber reinforcement and filling in polymer composites, looking for better properties, lower cost and appeal of sustainability. To do so, there are challenges and limitations to be overcome, such as the compatibility of fiber/matrix, the processing temperature, the moisture absorption and so on. The global auto industry, including Brazil, has led the use of composites containing natural fibers in commercial products and also serves as reference to other economic areas. In Brazil, the fomentation agencies have supported projects in natural fibers and their application in composites, with a significant amount of academic research groups working in this area. The aim of this study was to analyze the situation and trends in the manufacturing and use of materials containing natural fibers through patent analysis, with emphasis on sisal, and also to simulate a sisal/polypropylene composite in home appliance part. Materials, processes, practices and actors involved in these composites were mapped, based on indicators made from patent documents. The analysis of patent documents associated with natural fibers and their use in polymer composites, in the period of 1960 to 2009, demonstrated the growing importance of this subject in the world and particularly in Brazil. Among other results, it was found that Brazil holds the 16th position in patenting associated with natural fibers in general and the 12th place in patenting on the sisal fiber, despite being the world's largest producer of sisal and one of the largest producers of natural fibers in general. Companies and individuals assignees have the largest number of patent documents, when compared to universities and institutes. It was possible to make recommendations for the development of a sisal/polypropylene part through exploratory structural simulation. It was also needed to generate new data to enable simulation of the injection process.
Avaliação de programas sociais: uma análise do impacto do Bolsa Família sobre o consumo de alimentos
(Universidade Federal de São Carlos, 2012-01-24)
Baptistella, Juliana Carolina Frigo
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Ferro, Andrea Rodrigues
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https://lattes.cnpq.br/3432809140419477
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https://lattes.cnpq.br/9764000286649164
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Almeida, Alexandre Nunes de
This thesis aims to evaluate the impact of the Bolsa Familia Programme in food consumption of attended families. Given that the beneficiaries are poor and extremely poor families, we want to know what are the program s contribution for security of these families. We used data from the Pesquisa de Orçamentos Familiares 2008/2009, conducted by the Instituto Brasileiro de Geografia e Estatística, related to food consumption of the beneficiary (treatment group) and non-beneficiary (control group) families. Using propensity score matching method was possible to compare families very similar in their observable characteristics. We have found that the program contributes to increase food consumption of beneficiary families, once these families presented statistically and significantly higher expenses than non-beneficiary families. Also, he program collaborates to elevate the proportion of children and youth with normal corporal mass index, and increases the proportion of adults with obesity problems - these results can be indirectly associated.
Metodologia para monitoramento da mortalidade empresarial, e sua aplicação nas empresas de base tecnológica de São Carlos
(Universidade Federal de São Carlos, 2012-01-25)
Oliveira, Meire Ramalho de
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Ferrari Júnior, Roberto
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https://lattes.cnpq.br/1581806209106850
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https://lattes.cnpq.br/7648082931441728
This dissertation presents a proposed methodology for monitoring corporate mortality, and some results of its use to evaluate the mortality of technology-based companies in São Carlos, SP. The proposed methodology comprises 12 steps, and provides guidelines for defining the criteria for characterization of mortality (Stage I), selection of sources of information (Stages II and III), choice of the period of observation (Stage V), construction of the universe of research (Stages VII and VIII), determining rates of mortality (Stage XI), and identification of causes of death (Stage XII). The methodology also guides actions for routine monitoring of corporate mortality, and to compare results. The methodology was applied to evaluate the mortality of technology-based companies in São Carlos. The universe of the research was formed by the companies created by the year 2005, organized in four entries, namely ParqTec, GETEC, PIPE, and São Carlos. The criterion used for mortality was the formal closure, as detected in the JUCESP (Board of Trade of São Paulo State), and Internal Revenue Service taxpayer number (CNPJ). The period of observation of mortality ranged between 2006 and 2010. The following mortality rates were obtained: 5.2% of the companies from ParqTec established by 2005 formally ended their activities from 2006 to 2010. Similarly, the mortality was 6.8% of GETEC register, PIPE 0% and the rate of São Carlos register was 5.6%. The causes of death were explored by consulting the leaders of the companies closed. Due to the small number of responses, 8 questionnaires, the results regarding causes of death should not be regarded as conclusive. The methodology contributes primarily to guide the process of designing and implementing research to evaluate business mortality, to guide the choice of methodological alternatives, and comparing results.
Mecanismos reacionais dos sistemas Pb/H2SO4 e Pb/PbO2/H2SO4
(Universidade Federal de São Carlos, 2012-01-26)
Fernandes, Fabio Plut
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D'Alkaine, Carlos Ventura
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https://lattes.cnpq.br/8699821828310072
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https://lattes.cnpq.br/4836435433244717
The thesis will address a modern view of electrochemistry for the discharge and charge reactions of Lead Acid Batteries study. Even though these batteries have existed for more than 200 years, electrochemical mechanisms are still not fully understood. Recently, there is a discussion in Brazil about the need to improve energy accumulation processes that have not kept pace with the development of technologies such as electric car engines, telephony devices, computers. The production of lead-acid batteries is the only Brazilian energy accumulator. These Batteries continue to grow worldwide, increasing their forms of use, leaving the current car starter batteries and stationary stabilizers for hybrid cars (micro and medium hybrid), electric motorcycles, electric trains, mobile phone towers, oil platforms, among others. In this thesis, we started a critical review of the published data bibliography and its meanings. Most of the published work proposes that the mechanisms of unloading and loading the plates are by dissolving precipitation and would be similar. However, studies with negative and positive flat electrodes show almost no dissolution during these electrochemical reactions. Thus, the precipitation dissolution mechanism would not be justified. In the last decades, several studies already indicate that these mechanisms can be understood as solid-state mechanisms by adding the partial disruption phenomenon, which we will explore in this thesis. The literature data shows differences between the negative and positive plates mechanisms that need modern treatments because their discharge products are different films and from nanometer order. Finally, electrochemical and analytical studies will be carried out, with positive and negative flat electrodes. Experimental methods for negative flat electrodes are treated in detail, while positive flat electrodes still have a lot to be developed.