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Caracterização fenotípica, genômica e transcriptômica de parasitos Crithidia sp. LVH60a obtidos de pacientes diagnosticados com Leishmaniose Visceral
(Universidade Federal de São Carlos, 2023-11-09) Rogerio, Luana Aparecida; Maruyama, Sandra Regina Costa; https://lattes.cnpq.br/5719754923228879; https://lattes.cnpq.br/6652725921997633
Leishmaniasis is a group of neglected diseases that can be caused by over 20 species of protozoa from genus Leishmania. Since 1980, monoxenous trypanosomatids have been found in cutaneous and visceral leishmaniasis cases in both immunocompetent and immunocompromised patients. In Brazil, parasites of Crithidia genus were first reported in 2019 in a fatal case of human visceral leishmaniasis in Sergipe (SE), referred to as Crithidia sp. LVH60A. To characterize clinical isolates of Crithidia sp. LVH60A parasites obtained from a relapsing patient from the same region, this study aimed to characterize phenotypically and perform genomic and transcriptomic analyzes of these clinical isolates. In this regard, parasite cultures were analyzed for growth curves, morphological features, in vitro infectivity and in silico identification of virulence-related proteins in Crithidia sp. LVH60A genome. Genomic DNA was sequenced, assembled, and compared to other trypanosomatid species. Additionally, we assessed the difference in gene expression of parasites and human macrophages infected with different species of Leishmania and Crithidia using messenger RNA sequencing (mRNA-seq), employing a dual RNA-seq approach. Phenotypic analyses demonstrated that the Crithidia sp. LVH60A parasites were capable of replicate at temperatures of 25 °C and 35 °C, surviving and multiplying within different macrophage lineages. Morphological analyses revealed that the cellular body of Crithidia sp. LVH60A is rounded, possesses shorter flagella than L. infantum, and presents some differences when compared to the Crithidia fasciculata TCC039E strain. Genomic analyses showed that the clinical isolates from 2017 had over 98% nucleotide identity with the LVH60a C1 strain (Crithidia sp. LVH60A). Genome alignments of Crithidia sp. LVH60A and C. fasciculata strains showed an average nucleotide identity ranging from 92.7% to 94.8%, depending on the software used. Chromosomal rearrangements of Crithidia sp. LVH60A was also observed and validated when compared to C. fasciculata CfCl reference genome. The mRNA-seq results showed that infection with different parasite strains did not significantly alter the transcriptional expression of macrophages; however, slight changes were observed shortly after the infection. However, there was a clear difference in the expression of the trypanosomatid transcripts. Some differentially expressed transcripts are shared by strains of Leishmania spp. and Crithidia sp. LVH60A. These transcripts encode proteins that mediate responses to stress, nutrition, and host immune response evasion. Our results suggest that the Crithidia sp. LVH60A may be a new species within the Crithidia genus; however, more isolates of Crithidia sp. LVH60A need to be analyzed with a focus on population genomics to confirm this evidence.
"A gente tinha que se virar sozinha": processos educativos emergentes da andarilhagem de mulheres atletas de futsal
(Universidade Federal de São Carlos, 2023-11-09) Bianconi, Ana Cláudia; Souza Júnior, Osmar Moreira de; https://lattes.cnpq.br/9176123942671062; https://orcid.org/0000-0002-2915-5634; https://lattes.cnpq.br/2627342414497317
This research aims to identify, understand and describe the educational processes that emerge from the social practice of wandering among female futsal athletes. To participate in this study, five women futsal athletes who were part of the main team of the club Associação Sãocarlense de Futsal – ASF were selected from records in field diaries of a conversation circle. This is a qualitative research based on dialogicity and centered on subjects, which was developed from interviews with the participants, which were recorded and transcribed in field diaries. For data analysis, we chose to carry out an episodic analysis, in which excerpts from the interviews were selected, presented and discussed, in order to promote reflection around the episodes, associating them, when possible, with Freirean concepts. The results found point to the denunciation of processes of dehumanization and situations of oppression experienced by female futsal athletes when walking as they establish themselves professionally as athletes, and reveal educational processes of coping, mutual care, support, identity, which function as a foundation and contribute to the process of developing critical consciousness that leads to liberating praxis, based on the permanent and constant state of being subjects that they are being.