Análise in silico para definição de common core microbiano associado a condições com degradação de surfactante aniônico alquilbenzeno linear sulfonado (LAS)
Abstract
Linear alkylbenzene sulfonate (LAS) is the anionic surfactant of greatest economic
interest worldwide. Its biodegradation route involves reactions of fumarate addition,
β-oxidation, cleavage of the aromatic ring and desulfonation. Since this is a complex process,
the use of varied sets of microorganisms makes it possible to take advantage of different
metabolisms and enzymes in the degradation of LAS. However, there is a lack of literature on
the microorganisms that play a key role in the degradation of surfactant in biological reactors.
This study aimed to define the microbial common core associated with the degradation of
anionic surfactant linear alkylbenzene sulfonate in bioreactors, from data mining available in
public databases containing raw 16S rRNA gene sequences. Forty-six 16S rRNA gene
samples, obtained from 12 scientific papers, were analyzed using bioinformatics tools
combined with a workflow in R software. The most abundant genera among the samples were
Methanosaeta (4.6%) and C39 (2.3%). The genus SJA-28 was predominant in the common
core clusters and was present in six of the seven clusters constructed, followed by the
Reyranella and Christensenellaceae R-7 group, found in five common cores, and Candidatus
Magasanikbacteria, in four cores. By functional profile analysis, enzymes were found related
to the fumarate addition, desulfonation and β-oxidation steps of LAS biodegradation.
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