Bioprospecção de fungos filamentosos e leveduras para a produção de enzimas visando obtenção de etanol de segunda geração

Abstract

Ethanol has stood out as a promising alternative to reduce the environmental impact caused by fossil fuels. Sugarcane, being the main raw material for ethanol production in Brazil, becomes a source of renewable energy. In this context, second generation ethanol appears as a promising advance, making more efficient use of lignocellulosic waste. Therefore, the use of physical, chemical or biological pre-treatments on waste is necessary to release sugars for ethanol production. Biological treatment using enzymes produced by fungi has proven to be an efficient and economically viable approach. In this context, the present study isolated four strains of filamentous fungi and three yeasts from decomposing sugarcane bagasse and, after the strain selection stage, three of these filamentous fungi were isolated, together with three yeast strains previously bioprospected. by the research group, they were used in growth tests in medium enriched with sugarcane bagasse, in addition to enzymatic analyzes (CMCase and FPase). The results obtained revealed less favorable growth conditions in medium enriched with sugarcane bagasse compared to control media. However, the strains incubated in medium with bagasse demonstrated positive numbers of FPase and Endoglucanase production, where strain L3 presented the best result analyzed among CMCase (0.47 U/mL), and L2 obtained the best result for FPase (0.25 U/mL). These results indicate the potential of these isolated strains for the production of lignocellulolytic enzymes from sugarcane bagasse, which is relevant for the more efficient use of lignocellulosic residues in the production of second generation ethanol.