Navegando por Data de Publicação, começando com "2012-11-08"

Filtrar resultados por ano ou mês

Agora exibindo 1 - 2 de 2

Estudo histomorfométrico da necrose em tecido hepático de ratos: terapia fotodinâmica combinada com ablação a laser e terapia fotodinâmica com o fotossensibilizador Luzitin®
(Universidade Federal de São Carlos, 2012-11-08) Rego, Raquel Ferreira; Moreira, Fernando Manuel Araújo; https://lattes.cnpq.br/1809254923092721; https://lattes.cnpq.br/2622028338048884
Photodynamic Therapy (PDT) is known to be limited to applications in large volume tumours due to its limited penetration. Therefore, the PDT with the Luzitin® photossensitizer as well as a combination of PDT and laser surgery may constitute a potential to destroy bulk tumors. Thus, with the aim of proposing a minimally invasive treatment protocol involving the application of PDT for large tumors, the present study analyzed histomorphometrically necrosis resulting from both a combination of a laser ablation with PDT as PDT with the photosensitizer Luzitin® (a bacteriochloryn synthetic with greater potential for penetration into biological tissue) in livers of healthy rats. In the first study, 87 animals were divided into 2 groups: CO2 laser and diode laser. Each of these groups were subdivided into six subgroups: 1) only laser ablation, 2) administered the PS and ablated with laser, 3) only PDT (drug and ligth), 4) drug and light (PDT) followed by laser ablation, 5) ) laser ablation followed by a PDT and 6) drug, followed by laser ablation and ligth. For each subgroup, three types of photosensitization were used: topical 5- aminilevunic acid (ALA), intravenous ALA and intravenous Photogem®. Thirty hours after the different treatments, the animals were sacrificed and the livers removed for the histological and morphometric study of necrosis. The results showed that the effects of treatment were considerably improved when PDT was used in combination with laser ablation. For the group CO2 laser, the average depth of necrosis obtained showed a minimum difference between the studied conditions for the group photosensitized Photogem® and with an increased depth of necrosis after the combined procedures in comparison with the isolated techniques for the subgroups fotossensibilizados with ALA, especially treatment with PDT was performed before ablation by CO2 laser. In the diode laser group, subgroups with better performance were those in which the ablation was performed before PDT using intravenous photosensitizers and ALA topic. From these results, it is suggested that PDT and laser ablation can synergistically in the treatment of large tumors. In the second study, sixteen normal male rats were randomly divided into 4 groups with 4 animals each. Initially, all groups were intravenously photosensitizated with 2 mg/kg or 2.6 mg/kg of the drug and after 12 hours, a 1cm2 of area, in the right abdominal region, corresponding to the liver, was irradiated during 22 minutes and 13 seconds or16 minutes and 40 seconds. Groups are described following: 1) control group: photosensitized with 2mg/kg, but untreated, 2) treated group 1: irradiated with light dose of 100J/cm2 after photosensitization with 2mg/kg of 12hs and 3) treated group 2: irradiated with light dose of 70J/cm2 and photosensitized with 2mg/kg of Luzitin®, 4) treated group 3: photosensitized with 2.6 mg/kg of the drug and irradiated with light dose of 70J/cm2 Animals were sacrificed 30 hours after irradiation, except the control group, which were sacrificed 42hs after drug administration. Livers were removed and prepared for histological analysis. Moreover, macroscopic aspects of liver were observed. Results showed significantly more extensive necrosis and greater severity in treated group 1. Data suggested that, among the conditions studied, light dose suitable for PDT treatment of photosensitized tissue by Luzitin® is 100J/cm2 and that 30% of reduction in this value produces decay in PDT response, even with proportional increase of drug concentration in the organism.
O uso das tecnologias da informação e comunicação nas visitas escolares do centro de ciências de Araraquara: análise sobre a gincana tecnológica e investigativa de química
(Universidade Federal de São Carlos, 2012-11-08) Maruyama, José Antonio; Marques, Clélia Mara de Paula; https://lattes.cnpq.br/0451284088402660; https://lattes.cnpq.br/5287836991245906
This paper discusses the use of Information and Communication Technologies (ICT) in Chemistry Technological and Investigative Gymkhana, activity of the Sciences Center of Araraquara, an UNESP science museum. The objectives of this study were to investigate the use of these resources in the activity, the conception of these participants on such use and contributions to the Teaching of Chemistry. The research subjects were museum visitors students and data collection was done through a questionnaire answered at the end of the visits. The data indicated the participation of students from public, private and SESI system during the period of analysis. It was observed that the computer is materialized as a cultural tool and is used by visitors during GTIQ to perform the action for resolution of the proposed activity. Also making use of the internet to search for information, visitors indicated appropriate of this resource as a mediator of the action. So we can suggest that the use of technological resources changes the relationship of the students with the chemical contents, since they showed more interested when using equipment to which they have affinity (cellular and computers). Another important factor to be noted is that the use of ICT makes the search for information more dynamic, which, in the view of visitors, facilitated research and become more motivating. ICTs used during GTIQ played motivational role, arousing the interest of visitors by subjects and drawing attention to the same themes. ICTs being used in a investigative approach encouraged the active participation of visitors. Technological resources used during the activities of the Gymkhana had their potential exploited, helping assisting visitors in the problems solving presented and stimulating the use of the Internet as a learning environment.