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Estudo dos efeitos do bloqueio da integrina αvβ3 por uma desintegrina RGD recombinante na cicatrização de hérnia incisional em ratos
(Universidade Federal de São Carlos, 2014-06-27)
Oliveira, Claudio Ricardo de
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Araújo, Heloísa Sobreiro Selistre de
;
ttp://lattes.cnpq.br/4065824911933203
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https://lattes.cnpq.br/5091705745613726
Introduction: Incisional hernia (IH) is considered a connective tissue disease characterized by defective wound healing process, and it is found in high incidence after abdominal surgeries. Previous studies have shown that DisBa- 01 presents high-affinity to αvβ3, which can have wide therapeutic application and/or laboratorial in disorders related to collagen. Hypothesis: Considering the regulatory role of the vitronectin receptor, the αvβ3 integrin, in wound healing, we hypothesized that blocking this integrin would affect tissue repair. Objectives: To evaluate the DisBa-01 (a desintegrin RGD-recombinant by snake venom of Bothrops Alternatus) as a blockage of αvβ3 integrin on improvement of HI healing. Methods: IH was successfully induced in rats of Sham and DisBa-01 group. DisBa-01 group were treated with different doses (0.25; 0.5 and 1.0 mg/kg). A control group without surgery was included to analyze the surgery effects. Were evaluated the histology, zymography and gene expression of MMP-2, type I and III collagen and VEGF. Type 1A collagen were analysed by Western Blotting. Results: After 14 days, the presence of IH and hernia ring were observed in all sham-operated animals; in contrast, 100% of animals treated with 0.25 and 1.0 mg/kg of Disba-01 did not present visible signals of hernia and showed closed hernia rings. The surgery procedure strongly activated MMP-2 gene expression as well as its activity (P<0.05). Up-regulation of collagen 1 (col-1) and 3 (col-3) gene expression was also observed, DisBa-01 reduced the expression of col-I, however, did not affect col-III, while VEGF expression was down regulated at day14. In contrast, DisBa-01 significantly inhibited MMP-2 gene expression and its activity. Western Blotting analysis revealed the presence of Type1A collagen in all groups, however only DisBa-01 differed statistically of Control group. The thickness of the collagen layer in each of control group presented reduced. However, Sham and DisBa-01, there was a relative increase in all studied animals (P<0.05). Conclusion: These results indicate that αvβ3 integrin has an important role in the control of wound healing and its blockade modulate repair conditions, including IH. This information may be especially helpful for patients with recurrent IH.
Atuação e formação em estimulação precoce : caracterização da percepção dos profissionais em três instituições
(Universidade Federal de São Carlos, 2014-06-27)
Bobrek, Andreia
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Gil, Maria Stella Coutinho de Alcântara
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https://lattes.cnpq.br/1673770301699940
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https://lattes.cnpq.br/0727084816163852
In Brazilian context, the official guideline recommends that Early Stimulation have the purpose of providing the development of children with special needs. In the use of the institutions, this practice involves different types of professionals and of guided acquirements to children with risk to their development. The diversity of practices makes it relevant to describe the performance of the professionals that work in Early Stimulation programs as a way of enabling the improvement of the work carried out, aiming the harmonization of concepts, guidelines and practices. In this research, the purpose was to describe the performance of 13 professionals experienced in working at early stimulation programs, in three institutions, and to analyze the relationships established by these professionals between their professional practice and the knowledge acquired during graduation course. For this purpose, an exploratory study was carried out, whose data collection consisted of semistructured interviews with the abovementioned professionals and the collection of the institutions’ data through consults to the coordination and to the available documents. Regarding the results, the interviewed professionals conceived early stimulation as the provision of opportunities to the child’s development; and/or support to the Family or the prevention of risk for the development. The professionals considered it important for a good service in the early stimulation program the professional involvement and/or specific working conditions and/or partnership with the family. Regarding the difficulties faced in carrying out the work, the reports were focused on the lack of familiar participation/involvement, the inadequacy of work conditions and the work with the child itself. Regarding the preparation/training that the Higher Education Institution (HEI) offered for the work in early stimulation program, eight interviewed professionals informed that the graduation course offered the training on the Early Stimulation; however, half of them related that this training was inadequate/superficial. From the thirteen professionals, five declared that the HEI did not offer preparation/training to work with early stimulation. From all the professionals, tem considered that the HEI could have offered better training, even those that offered some; from those tem, five declared that there should be better articulation between theory and practice.
Estudo de inserção de jogos computadorizados como recurso complementar ao ensino de leitura
(Universidade Federal de São Carlos, 2014-06-27)
Marques, Leonardo Brandão
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Souza, Deisy das Graças de
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https://lattes.cnpq.br/4404800720856419
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https://lattes.cnpq.br/3705407022339177
The literature cites educational games as a procedure that increase the spontaneous engagement in educational task and assist the retention of what was learned. Choice procedures and the identification of preference among different teaching procedures can be taken as an indicator of further spontaneous engagement .Were proposed two complementary studies to investigate the influence of electronic games in the learning of reading: The Study 1 tested an application of a software and a procedure for teaching reading in large-scale (a school environment) with 11 participants; the Study 2 incorporated this same teaching procedure in a game (gamified version) and assessed the preference between the game and the standard condition of teaching (without game) among 27 elementary students. The results indicated that for both conditions (standard and gamified) the teaching program was effective in teaching basic reading skills. Most participants presented preference for the gamebased teaching condition format. The gains in reading performances were kept in this condition and the number of repetitions of the teaching procedure was also lower in game situation. It has not been possible to identify a preference between game or the conventional procedure for about 30% of the participants. Participants who showed preference for Game condition tended to repeat less blocks of training components of each teaching session. A task who asked the students to choose between the default condition and return to the classroom indicated that participants with preference for Game condition remained engaged in teaching procedure when was given the opportunity to return to the classroom. The discussion focuses on the parameters used in the choice task, pointing manipulations that optimizes the method to measure the preference, in order to make it more sensitive to different measures of the game as a motivational factor.
Estudo dos Doryctinae (Hymenoptera: Braconidae) em áreas de mata da região sudeste brasileira
(Universidade Federal de São Carlos, 2014-06-27)
Pinto, Clóvis Sormus de Castro
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Dias, Angélica Maria Penteado Martins
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https://lattes.cnpq.br/8637344058859147
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https://lattes.cnpq.br/1027467404762561
The Hymenoptera parasitoids have great importance in maintaining of the environmental balance in the population control of many insect hosts. For the present study, was made a faunistic survey of Doryctinae from collected at INCT- Hympar Sudeste. Samples were collected in 32 municipalities each one of them with five sampling points, excluding São Luís do Paraitinga city with seven points, all with different sampling efforts. To capture of insects, Malaise, Moericke and swep of vegetation traps were used. The total number of individuals captured was 8,210 specimens, distributed in 32 valid genera, during the period between 2009 and 2011. Jundiaí city had the largest number of specimens of Doryctinae, with 1,411 individuals and occurrence frequency of 17.18%, and also the largest genus richness, with 15 in total. Seven municipalities had a unique genus to the location. Heterospilus Haliday was the dominant genus with 83.11% of the total collected specimens (6,824). The collection method with which most individuals was captured was swep of vegetation with 47.24% of the total Doryctinae collected by this technique (1,239). Although avoidance about 7% of the biome, and to be quite fragmented, the Atlantic Forest concentrated at Southeastern region in Brazil presented very abundant and rich in genus of Doryctinae, being found in this work two of them endemic to the Atlantic Forest: Percnobracon Kieffer and Rimacollus Marsh. Six new species of Hecabolus Curtis, a new species of Lissodoryctes Marsh, and a new species and first record of Nervellius Roman, and three new first records of Megaloproctus Schulz were published during the development of this work.
Caracterização dos fatores para implantação do processo de Sales and Operations Planning (S&OP): um estudo multicaso
(Universidade Federal de São Carlos, 2014-06-27)
Pedroso, Carolina Belotti
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Silva, Andrea Lago da
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https://lattes.cnpq.br/4287346156065964
In today's dynamic market scenario to develop skills for quick and assertive decision making is still one of the challenges faced by companies around the world. In order to assist managers to meet the demand in a competitive way, a new approach to integrated management of business processes has emerged in recent years the S&OP (Sales and Operations Planning). This approach encompasses the master production planning, demand planning and managing the flow of information, resulting in a unique planning to the company. Thus, the purpose of this research is to characterize the success factors for S&OP implementation in three companies that are in distinct S&OP maturity level. Firstly, a Literature Review was conducted about Sales and Operations Planning (S&OP), in order to identify relevant points in literature, for this research. For this porpouse, it was used the Systematic Literature Review principles. The empirical part of the research included the method of multi case study in which three organizations that have already deployed the S&OP, but are in different stages of maturity, were investigated in order to clarify different perspectives of the research problem and find common characteristics. After conducting semi-structured interviews, that were used as a research tool, it was used the technique of content analysis to survey the main features of the implementation of S&OP process. From the results, it was possible to develop a set of factors for companies wishing to deploy the process, in which the main determining factors of success were: the involvement of senior management, cross-functional integration, metrics and performance monitoring, information systems and understanding of the process or training. Conversely, the main barriers identified in the study included the culture of silos and inadequate technology and information systems. It is assumed that both external variables such as culture and local economy and internal variables, such as the production strategy adopted, can influence the process of S&OP, elements yet not explored by the literature about the subject.
Procedimentos para gestão de ocorrência de inundações em cidades de pequeno e médio portes com o uso de SIG e logística humanitária. Estudo de caso: cidade de Jahu, SP
(Universidade Federal de São Carlos, 2014-06-27)
Feltrin, Talita Fernanda
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Raia Junior, Archimedes Azevedo
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https://lattes.cnpq.br/6413793013018019
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https://lattes.cnpq.br/9165563114411890
The scenarios of natural disasters are a reality today, and tend to become increasingly frequent in view of climate change that generate abnormal conditions intensively, since the increase of people living without adequate infrastructure susceptible to the ocurrence of disaster areas, even increasing number of vehicles on the streets, which may cause and/or exacerbate the intensity of disasters. In Brazil, municipalities suffer from landslides, strong winds, drought and floods mainly. Through emergency situations, the logistics network is fundamental to manage the supplies should be stored in strategic locations and care teams should be positioned near the sites affected by the disaster and may meet in a timely manner, especially sites that were affected with greater intensity. In view of this, it is necessary, through the use of Geographic Information System (GIS) map areas of risk, define and quantify the strategic points that will support the management of the Humanitarian Logistics, in order to ensure lower response time, minimize risk, and optimal performance in cases of emergency contingencies. Based on these aspects, this research aimed to develop a method of disaster management, by analyzing the areas of floods risk, through a Geographic Information System, seeking to identify, from this mapping, the locations that serve as supply warehouses and central of assistance for care teams. GIS, as well as LSS (Logistics Support System), tool that was used in this research, assisted in the preparation of the flow procedures for the logistics management of flooding for small and medium cities. Through simulations, could observe that for the case of a mapping realized using models and mathematical methods and due to lack of data registered along the basin studies, that was used the Method Muskingum-Cunge that best applied to types of data collected. Whit this, the study focused only on the river macrodrainage Jahu. To determine the strategic locations were considered urban facilities such as schools, kindergartens, hospitals, and others to act as local shelters and warehouses for humanitarian assistance. With the use of LSS could be analyzed that the tool allows organizing, quantify and qualify supplies received and distributed, to avoid the lack of essential surpimentos unnecessary and excessive supplies. Finally, from the use of LSS and GIS tools as well as the development of procedures for the management of humanitarian logistics is possible prepare the contingency plan for the occurrence of flooding to attend the Federal Law 12,608, with the main objective to reduce the time of decision making by Civil Defense and service teams.
Eletrodo de diamante dopado com boro para a determinação voltamétrica/amperométrica de antihelmínticos e anti-hipertensivos
(Universidade Federal de São Carlos, 2014-06-27)
Lourenção, Bruna Cláudia
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Fatibello Filho, Orlando
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https://lattes.cnpq.br/9859737944357808
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https://lattes.cnpq.br/0205548799348406
In this work it was developed, firstly, one method for determination of anthelmintic albendazole (ABZ) using differential pulse voltammetry (DPV) and the boron doped diamond (BDD) electrode catholically pretreated (BDD-CPT). The corresponding analytical curve showed linear response in the concentration range from 0.08 to 8.36 ^mol L-1 with detection limit (LD) of 0.06 ^mol L-1. The proposed method was applied in the determination of ABZ in three pharmaceutical formulations with similar results those obtained using a comparative method. The second part of this work it was to develop two electroanalytical procedures using the amperometry technique coupled to a flow injection analysis system (FIA) for the individual determination of two anthelmintic, the ivermectin (IVM) and levamisole (LVM), where it was used the BDD electrode anodically pretreated (BDD-APT) for LVM and BDD-CPT for IVM. Good linear ranges were obtained for the respective analytical curves with values of 0.60 to 50.0 ^mol L-1 for IVM and 0.01 to 6.00 ^mol L 1 for LVM with LD of 0.30 ^mol L 1 and 1.0 nmol L 1, respectively. These analytes were determined in samples of commercial pharmaceutical formulations and synthetic urine and the results obtained are in agreement with the results obtained using two comparative methods (one for each analyte). The analytical frequencies for each method were 48 and 56 determinations per hour for IVM and LVM, respectively. Another procedure was developed for simultaneous determination of two anthelmintics LVM and albendazole sulfoxide (ABZ-SO) in synthetic urine samples using a FIA system with multiple pulses amperometry (MPA) detection and the BDD-APT electrode. This method was applied for directly determination of ABZ-SO and for indirectly determination of LVM. The employed sequence of dual-potential waveform as a function of time, previously optimized, were Edet1 = +1.40 V / ms 200 (pulse potential at which occurs only ABZ-SO oxidation) and Edet2 = 1.90 V / 200 ms (pulse potential at which the oxidation of both anthelmintic, ABZ-SO and LVM, occurs). The analytical curves showed linearity from 2.00 to 100.0 ^mol L-1 (ABZ-SO) and 0.03 to 1.00 ^mol L-1 (LVM), with LD of 1.00 ^mol L-1 and 6.0 nmol L-1 for ABZ-SO and LVM, respectively. Samples of synthetic urine doped with two different concentrations of ABZ-SO and LVM presented recoveries from 93.3 to 100%. The procedure was simple, accurate and fast, with an analytical frequency of 53 samples per hour. In the last one procedure it was developed a method for simultaneous determination of hydrochlorothiazide (HTZ) and enalapril (ENP) (antihypertensives) using a FIA-MPA system and BDD-CPT electrode. The FIA-MPA method was also proposed based on the direct determination of HTZ and indirect of ENP. The sequence application of the pulses as a function of time, were, Edet1 = +1.50 V / ms 150 (pulse potential at which HTZ occurs) and Edet2 = +1.80 V / ms 150 (pulse potential at which oxidation of both analytes (HTZ and ENP) occurs). The analytical curves presented linearity from 0.40 to 8.00 ^mol L -1 to HTZ and from 0.03 to 1.00 ^mol L-1 to HTZ and LD obtained for HTZ and ENP were 0.20 and 0.01 ^mol L-1, respectively. It was analysed three pharmaceutical commercial samples containing both analytes and the results obtained using the FIA-MPA method are in agreement with those obtained using a comparative method (HPLC) with a confidence level of 95%. The procedure was simple and fast, with an analytical frequency of 89 samples per hour.
Índice sustentável de bem-estar econômico: uma proposta de mesuração para os municípios paulistas
(Universidade Federal de São Carlos, 2014-06-27)
Silva, Jonathas de Melo Cristovão
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Ferro, Andrea Rodrigues
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https://lattes.cnpq.br/3432809140419477
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https://lattes.cnpq.br/6244697096298561
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Gomes, Alexandre Lopes
The objective of this research is incorporate environmental conditions in a synthetic index of well-being for the counties beyond traditional dimensions of income, education and health in order to contribute to the diagnosis of the quality of life. Among the many challenges presented to policymakers, one of the applicants is a relative scarcity of public resources to meet a growing demand for goods and services. Another set of challenges is the difficulty of making more accurate diagnoses of problems and needs of the population, making the choice of priorities for action no trivial task. Analytical tools that can assist planning and monitoring of public policies are essential to establish alternatives for expanding the economic and social well-being, integrating economic growth and environmental sustainability. To meet the proposed goal, this work uses MIQL-M - Multidimensional Index of Quality of Life, a multidimensional index composed by the capabilities, concepted by Sen, still incorporating environmental sustainability indicators present in IAA- Index Environmental Assessment, established by the State Secretariat of Environment of São Paulo. Obtained a new index of well-being, perform a comparison of the systems of the municipalities according to the relative position obtained with and without the environmental variable. The main results show that the best municipalities assessed by modified MIQL-M did not show good results when environmental sustainability is considered. The districts with low quality of life were more dispersed throughout the territory and more concentrated in the east and south of the state. The municipalities that have improved their positions when the environmental factor is considered were more concentrated in the area of environmental preservation, and the relative improvement did not guarantee high degree of development since the evaluation considers other socioeconomic factors. Those who have lost positions in the ranking were located in regions where industrial dynamics is strongly present in the state. However, the global Moran's I test rejects the hypothesis that municipalities with high industrial participation are influencing the quality of life of neighboring regions due to spatial proximity.
Avaliação das habilidades sociais e variáveis sociodemográficas de adolescentes com e sem indicadores de depressão
(Universidade Federal de São Carlos, 2014-06-27)
Campos, Josiane Rosa
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Del Prette, Almir
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https://lattes.cnpq.br/2191226494887247
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https://lattes.cnpq.br/4613953640278499
Researches indicate that adolescents who have diagnostic or indicators of depression present many difficulties to start and maintain social relationships. Frequently, they are rejected by the society, which products a clinically relevant ill-being. The literature points associations between depression among adolescents and low repertory of social skills and, on the other hand, adolescents without depression indicators would present higher frequency of social skills. Therefore, the presence of social skills in the adolescents repertory would constitute a protection factor, and the low frequency, risk to the development of depressive symptoms. However, although this hypothesis is plausible at the theoretical point of view, empirical studies that investigate the theme depression in adolescence and social skills are few, especially in the Brazilian context. Studies found in the literature also didn t insert socio-demographic variable like age, gender, socio-economic status at the researches about social skills and depression in adolescence, which are also considered variables associated with depressive symptoms and social skills. In this sense, the general objective of the present research was to characterize, compare and predict the social skills repertory of adolescents with and without depression indicators, considering socio-demographic variables. This thesis is composed by four studies where one of them is theoretical and the other three are empirical. The first study, of theoretical nature, reviewed the last 20 years of scientific literature on the theme of social skills and depression in adolescence. The second characterized and compared the social skills repertory of 103 adolescents with and 103 without depression indicators, considering differences in gender. The third one suggested a model of multiple logistic regression, where the social skills of 642 adolescents (103 with depression indicators and 539 without it) were investigated as protection/risk factors and the predictive power in indicating depression in adolescents. The fourth study compared two samples: a general one (composed by 103 adolescents with depression indicators and 539 without it) and another one, nonclinical (composed by just 539 adolescents without depression indicators), aiming to check the impact of this control on the social skills scores and the influence of gender and social-economic status on the results. The participants of all empirical studies were 13 years old in average. The instruments used in these empirical studies were the Inventory of Social Skills for Adolescents IHSA Del Prette, Inventory of Infant Depression and Brazil Criteria. Data were collected at municipal schools, of a city in the interior of São Paulo state. Descriptive and inferential statistical analyses were used. In the first study were found 28 articles, the biggest part of them were empirical , done outside the country, with pre-experimental design, emphasis on the use of instruments of self-report, aiming to predict variables and categories of social skills investigated were: assertiveness, communication and approach. In the second study, there was difference significantly statistical in the general score, on the classes of self-control, civility, and assertiveness in the female sample; in the case of male sample, there was difference significantly statistical just for the categories of social resourcefulness. In the third study, the skills more frequent of empathy and self-control as well as 12 years old were shown as risk factors. The model presented low predictive power. In the fourth study, the results pointed debasement of the overall score and the social skills of empathy and self-control, just for the female sample. The implications limitations and reach of these data are discussed.
Efeito do tempo entre passes na interação recristalização-precipitação de um aço inoxidável austenítico com alto teor de nióbio e nitrogênio
(Universidade Federal de São Carlos, 2014-06-27)
Silva, Mariana Beatriz dos Reis
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Jorge Júnior, Alberto Moreira
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https://lattes.cnpq.br/6360362248108490
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https://lattes.cnpq.br/8849710516030183
In this work was studied the influence of interpass time on the recrystallizationprecipitation interaction occurring during the thermomechanical processing of ISO 5832-9 austenitic stainless steel, which has high contents of niobium (0.28%) and nitrogen (0.35%). The simulation of the industrial processing was carried out through hot torsion testing with multiple deformations in continuous cooling, in which the interpass time ranged between 5 and 50 s. The obtained plastic flow curves showed that shortest interpass times lead to increased levels of stress during straining and that the non-recrystallization temperature for this steel is close to 1100°C. The microstructural evolution was investigated by interrupting the tests on certain deformations before and after Tnr, and for this it was used the microscopy techniques of optical, transmission electron and scanning electron associated with electron backscattered diffraction. Small interpass times resulted in a larger volume fraction of small precipitates (< 50 nm) and smaller grain sizes. It was observed that dynamic recrystallization also occurs, and that these smaller precipitates were effective in pinning grain boundaries and dislocations (continue...).
Identificação de regiões genômicas e genes candidatos posicionais e funcionais para características de eficiência alimentar em bovinos da raça Nelore
(Universidade Federal de São Carlos, 2014-06-30)
Oliveira, Priscila Silva Neubern de
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Regitano, Luciana Correia de Almeida
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https://lattes.cnpq.br/2168962905598747
This thesis has been divided into three chapters for better understanding of the experiments. The first chapter refers to a brief Literature Review, with the main concepts and justifications for this work. The second chapter discusses the strategy of positional and functional candidate genes for traits of feed efficiency in Nelore cattle, and the third chapter presents a genome-wide association study for feed efficiency traits in the same population. Feed efficiency is an important production trait in beef cattle production systems. The aim of this study was to identify genes/QTLs associated with feed efficiency traits in Nelore cattle genotypes using the Illumina BeadChip BovineHD (770K) of 593 Nelore steers. The traits analyzed were: average daily weight gain (ADG), dry matter intake (DMI), feed conversion (FC), feed efficiency (FE), residual feed intake (RFI), efficiency of maintenance (EM), efficiency gain (EG), partial efficiency of growth (PEG) and relative growth rate (RGR). The first strategy discussed in this work, using candidate genes, investigated the association of Neurogenic differentiaton 1 (NEUROD1), Kv channel interacting protein 4 (KCNIP4), and Vascular endothelial growth factor C (VEGFC) genes, with production traits in the same population. Our results are in according to literature that indicate NEUROD1, KCNIP4 and VEGFC as candidate genes for feed efficiency; and this study is the first to suggest the NEUROD1 gene as a candidate for gene backfat thickness, body weigth and metabolic weight in Nelore cattle. In the genome-wide association study some QTL regions identified in this study minimally overlapped with QTL regions previously reported for feed efficiency in Bos Taurus cattle, and harbor genes with biological functions related to metabolic processes, lipid and protein metabolism, energy generation and growth. A comparison with published results indicates that different QTL and genes may be involved in the control of feed efficiency in this population of Nelore cattle. The validation of the results obtained in this work in independent populations may contribute to elucidation of the mechanisms will involved in the variation of feed consumption and specific improvement in Nelore programs.
Localização, distribuição e orientação dos quimiorreceptores de O2 branquiais e o controle neural periférico dos reflexos cardiorrespiratórios de um teleósteo de respiração aérea, o bagre-africano, Clarias gariepinus
(Universidade Federal de São Carlos, 2014-06-30)
Belão, Thiago de Campos
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Rantin, Francisco Tadeu
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https://lattes.cnpq.br/3546242299713690
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https://lattes.cnpq.br/8876132456472368
The African sharptooth catfish Clarias gariepinus is a facultative air-breather with the ABO present in the 2nd and 3rd gill arches. Like other bimodal respirators, the African catfish presents cardiorespiratory adjustments to survive low PwO2. These regulations are modulated by gill O2 chemoreceptors monitoring the PwO2 and/or the PaO2. The present study analyzed the distribution, location and orientation of the branchial O2 chemoreceptors of C. gariepinus, and the role of these receptors on the cardiorespiratory function and the modulation of the heart autonomic control. C. gariepinus were divided into two experimental groups (Control GC, intact fish, and Experimental GD, with the 1st gill arches excised) and their cardiorespiratory variables were analyzed during graded hypoxia with and without access to the atmospheric air, internal and external NaCN injections and autonomic nervous system antagonists. During hypoxia without access to air, both GC and GD showed increases in the respiratory variables (fR, VT and 𝑉 𝐺 ). However, the GD displayed higher values, since normóxia until severe hypoxia, to compensate the decrease in the EO2 resulting from the ablation of the 1st gill arches. Furthermore, the decrease in EO2 was responsible for the decrease in 𝑉 O2 and, consequently, for the increase of the PcO2 from 61 mmHg (GC) to 79 mmHg (GD). The GD featured bradycardia similar to GC, however, their values were higher in all PwO2. In hypoxia with access to air, both groups showed decrease in the fR and VAMP in the mores hypoxic tensions. This was attributed to the increases in the fRA in GC which compensate the O2 uptake. However, the GC increases in fRA were attenuated by the excision of the 1st gill arches. Moreover, both groups developed bradycardia pre-RA (typical of aquatic respirator) and tachycardia post-RA (typical of aerial respirator). Internal and external NaCN injections caused bradycardia in both groups, while the internal injections increased the ventilator variables only in GC. Thus, it is concluded that C. gariepinus possess branchial O2 chemoreceptors modulating the fH distributed by all gill arches, monitoring both PwO2 and PaO2. Conversely, fR and VAMP are modulated by receptors located in the 1st gill arches monitoring exclusively the PaO2. Furthermore, NaCN internal injections elevated more the fRA in GC than the external ones, but with low effect on the fRA of GD. Thus, the fRA is primarily modulated by O2 chemoreceptors located in the 1st gill arches monitoring, predominantly, the PaO2. Injections blocking the autonomic system to the heart showed that the modulation of pre-AR bradycardia and post-AR tachycardia occurs due to variations in the vagal cholinergic tonus, which is elevated during bradycardia and reduced during tachycardia. The adrenergic tonus, in spite of the decrease in the post-AR, is lower than the cholinergic, suggests a "reflex adrenergic" arising from the peripheral vasoconstriction, important to optimize the perfusion of the ABOs.
Produção de compostos antimicrobianos por Paenibacillus polymyxa RNC-D: otimização das condições de cultivo, purificação e caracterização dos bioprodutos
(Universidade Federal de São Carlos, 2014-06-30)
Serrano, Nadja Fernanda Gonzaga
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Sousa, Cristina Paiva de
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https://lattes.cnpq.br/9002619114161319
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https://lattes.cnpq.br/3313420666550077
The increase in the production of antimicrobial metabolites by Paenibacillus polymyxa RNC-D was appraised through the study of cultivation variables. Two process variables, namely the glucose and inoculum concentrations, were evaluated in different levels (5 to 40 g/l, and 2.5% to 5.0% v/v, respectively), and their effects on biomass formation, minimal inhibitory concentration (MIC) against Escherichia coli and surface tension reduction (STR) were studied. The fermentation process was firstly carried out using non-optimized parameters, where the dependent variables biomass, MIC and STR reached the values of 0.6 g/l, 1.000,0 μg/ml and 18.4 mN/m, respectively. The optimum glucose (16 g/l) and inoculum concentrations (5.0% v/v) were defined in order to maximize the biomass formation, with low value of MIC and large STR of extract. Under these conditions, a biomass of 2.76 g/l, MIC of 15.8 μg/ml, and STR of 14.58 mN/m were predicted by the model. Data attained by experiments using optimized settings showed the following values: biomass 2.05 g/l; MIC 31.2 μg/ml; STR 10.7 mN/m. Thus, the percentage of improvement for each target response was: biomass 241.6%; MIC 96.88%; STR 41.85%. It was found that high concentrations of glucose substrate, although reflected in an increase in bacterial biomass, inhibited the microbial secondary metabolism, resulting in a low production of biomolecules associated with high values of MICs. Thus, initial concentrations of glucose and inoculum are shown as variables of strong influence in the production of antimicrobial metabolites by P. polymyxa RNC-D. Through the methods of experimental factorial design and surfaceresponse followed by graphical optimization it was possible to determine the optimum operating condition to achieve both maximum biomass and RTS as well as and lowest possible values of CIM. The validity of the proposed model was verified and confirmed. This is the first study on the optimization of culture conditions for the production of antimicrobial metabolites by P. polymyxa RNC-D, and constitutes an important step in the development of strategies to modulate the production of antimicrobial molecules by this microorganism in elevated levels. Novel antimicrobial compounds were isolated from the fermentation broth of P. polymyxa RNC-D, here named total extract (TE). It was possible to verify the presence of lipopeptide and peptide active compounds through enzymatic assays made with ET. Total extract was subjected to a two-phase system, resulting in lipopeptide extract (LPE) and aqueous fraction (AF). According to the results of bioassays, LPE has broad-spectrum activity against Gram-positive bacteria, Gram-negative bacteria and fungi. The mass spectrometry analysis of PLA revealed the existence of a novel compound that was named polycerradin. The purification of a novel antimicrobial peptide (AMP) from the AF was carried out by using chromatography. The compound was active against Gram-negative bacteria. Nterminal analysis determined the amino acid sequence, as well as MS / MS analysis confirmed the primary structure of this new compound. This research reports firstly the production of PAM PpRNCD that has an unusual amino acid in its constitution. It is an unprecedented fact considering the bacterial specie P. polymyxa. In terms of molecule size, PAM PpRNCD can be considered one of the smallest active natural peptide reported to date. It was also possible to isolate from FA the depsipeptides IL-F04a (m/z 883), LI-F04b (m/z 897), LI-F03a (m/z 947) and LI-F03b (m/z 961) previously described in the literature. The photoluminescence study of the LPE, TE, AF in both at room temperature (RT) and low temperature (T = 8K) was performed. In addition, this technique was applied to evaluate the action of the ELP on Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 29212, Shigella sonnei ATCC 1578 and Candida albicans ATCC 10231 in two different situations: (a) immediately after mixing LPE with the bacterial and fungus cell suspension, and (b) after thirty minutes. The photoluminescence emission was collected by a triple spectrometer (three diffraction gratings) T64000 model from Jobin Yvon, equipped with an optical microscope. For the detection of the radiation emitted by the sample we used a CCD camera (charge coupled device) cooled by liquid nitrogen. The slits of the spectrometer were adjusted to produce a spectral resolution of the order of 10-4 nm. The excitation source used was the line of 457 nm (violet) from an argon laser. The behaviors here observed indicate a strong potential for applications in biosensors as well as molecular markers.
Tribunal constitucional: uma proposta da Ordem dos Advogados do Brasil na constituinte de 1987/88
(Universidade Federal de São Carlos, 2014-06-30)
Serotini, André
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Souza, Márcia Teixeira de
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https://lattes.cnpq.br/2846115924284615
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https://lattes.cnpq.br/1395564531728373
The aim of this research is the performance of the Bar Association in Brazil concerning the Constituent Process of 1987/88, which was an important political time at the end of the twentieth century in Brazil. Marked by an ambivalence of vocations, the Brazilian Bar Association managed to move self confidently between corporate and institutional interests. It is by taking advantage of this characteristic and its history, mainly related to conflicts of legal and democratic nature, that we are motivated to immerse into a universe of this sui generis institution, drawing Law and Policy closer in such a way that they seem inseparable. The chosen arena, the Constituent of 1987, brings all the characteristic features of a good political novel , full of alliances and strategies. The topic that we report as ideal is the analysis of questions which involve setting up a Constitutional Court, proposed by the association, which went through procedures in the Subcommittee of the Judicial Power and the Public Prosecutor. One of the main obstacles was the centuries-old tradition of the Supreme Constitutional Court, an organ of the Judicial Power, which would be substituted, if the progressive intent of the Bar Association in Brazil had been successful. Moreover, taking this into account, we attempt to clarify which factors contributed to this frustration, even knowing that approximately 75% (seventy five percent) of the constituents belonging to the Subcommittee were professionally declared as lawyers.
A teoria de gêneros na formação inicial de professores de língua inglesa: investigando contribuições para o desenvolvimento do conhecimento docente
(Universidade Federal de São Carlos, 2014-06-30)
Campos-Gonella, Cristiane Oliveira
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Augusto-Navarro, Eliane Hercules
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https://lattes.cnpq.br/5716027350340906
This study aims at investigating the effects of using the swalesian genre analysis theory in the process of teacher education. The participants are pre-service teachers of English as a foreign language, who are in the third-year of their undergraduate course in Languages and Literature. The theoretical frameworks of this research combine the theory and pedagogy of genres and the theory of teacher education, the latter in light of the teacher cognition studies. The investigation can be classified as essentially qualitative, with an interpretive and ethnographic perspective. The data analysis process reveals that the use of the genre theory in language teacher education can be considered an adequate instrument for both, a better understanding of the text, ultimately promoting a better understanding of the situated use of the target language by student-teachers, as well as an exercise of methodological choices and implementation of education. These aspects, together, converged on an improvement of teacher knowledge. In this sense, this study contributes to the affirmation of the genre theory in English language teacher education by providing both, subsidy and varied perspectives to the process.
Existência e multiplicidade de soluções para uma classe de equações de Schrödinger com expoente supercrítico
(Universidade Federal de São Carlos, 2014-06-30)
Moreira Neto, Sandra Imaculada
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Miyagaki, Olímpio Hiroshi
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https://lattes.cnpq.br/2646698407526867
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https://lattes.cnpq.br/1983605058147243
Ação ergonômica e estratégias de operações: proposta de integração na prática
(Universidade Federal de São Carlos, 2014-06-30)
Gonçalves, Juliana Machion
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Camarotto, João Alberto
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https://lattes.cnpq.br/1427853773681255
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https://lattes.cnpq.br/4059272741028061
This research has, with main objective, the construction of activity ergonomics management model that is related operations strategies, developed from practical experience in the company with metal - mechanics production of white goods, involving a group of approximately 1.500 people and 400 workplaces, aimed at structuring an ergonomic action, based on the activity ergonomics, which it is aligned with the operations strategy and understanding the consequences (positive and negative) about ergonomics action, the perception of the operators and managers of the company, the relationship between health and work. The construction involved a participatory process the transformation of the working conditions and workplace conception. The theoretical-methodological approach has the assumptions of ergonomics work analysis and action research were used to construct the model based on the activity ergonomics of operations strategies related in order to emerge among all the actors involved greater understanding of the work, its determinants and constraints, as well as the instrument to represent the perceptions of the actors of this process and its contradictions. The demand started from high rates of removal by musculoskeletal disorders in the area of manufacturing company. The data were indicators that guided the certificates and clearances. Training, analysis / diagnosis and projects were the steps of action research. Relation about training, a ergonomics committee was formed with the performance of health professionals, process engineers and production supervisors. These actors were trained and helped to survey the descriptions of the task, the constraints involved, the rules and expected results and project items, so they would get to the point of view of activity and operations strategies, to further suggest recommendations necessary. Moreover, the process of analysis / diagnosis was developed from the ergonomics law (NR 17) with assumptions of ergonomic work analysis (AET). Finally, the stage was set designs from the results of a summary of the analysis / diagnosis. Every problem encountered was given a solution, aligning with operations strategies (organization of work, performance measures, process technologies, and human resources) through implementation of breaks, job rotation, procedure for development projects, order to modify the organization of work and technical and organizational design and in case devices, design of design processes and correction operations. From there, the team can count on financial support from the direction of the company and a specialized and skilled in analyzing work situations, innovative practices and active participation of the operator internal technical structure to generate credibility and consistency in actions that enhance and stimulate quality of work life.
Diferentes modelagens de análise da bacia hidrográfica do Ribeirão-Araras, São Carlos, São Paulo, para a espécie Antilophia galeata (Lichtenstein), 1823 (Aves, Passeriformes, Pipridae)
(Universidade Federal de São Carlos, 2014-06-30)
Ruy, Leonardo Seneme
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Pires, José Salatiel Rodrigues
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https://lattes.cnpq.br/2276704131769342
The present study aimed to analyze and discuss the results of the analysis of the landscape at different scales and levels of details of its structure interfere in predicting the presence and population size of a species and environmental quality of a unit of study. For this, executest were three main steps: 1) The choice of the species of study; 2) Review the unit of study with different models for the species of study; and 3) Propositions for the unit of study. The choice of the species of study was based on a questionnaire to various ornithologists, where we tried to select the most suitable species for the study. For the analyzes of the study unit was used , on the conception of Landscape Ecology , tools such as scanning a satellite image obtained by Google Earth ® and landscape analysis by MapInfow ® program. Propositions for the unit of study was based on analysis of the model considered more real and critical to the species of study aiming at establishing minimum viable conditions for the establishment and maintenance of the species studied. This specie of study was chosen Antilophia galeata, the unit of study is located in the municipality of São Carlos (SP), and the whole basin of Ribeirao das Araras. This study showed that one must have a very great care in analyzing the most appropriate model for a given species, because the way that the landscape can be analyzed can generate completely different results and sometimes lagged the actual situation, as evidenced by this paper.
Trabalho padronizado e equipes semi autônomas: adaptação, aplicação e análise de um modelo a partir de múltiplos casos em uma empresa do setor químico
(Universidade Federal de São Carlos, 2014-06-30)
Renó, Gece Wallace Santos
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Truzzi, Oswaldo Mário Serra
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https://lattes.cnpq.br/5005536921435787
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https://lattes.cnpq.br/3591115081135676
The manufacturing companies are increasingly investing time and attention to improving its operating performance by accepting that this is one of the few ways to be competitive in the medium and long term and thus leaving the short-term vision where operational improvements are mainly evaluated as cost reductions of production and labor. The manufacturing management must seek the proper standardization of operations, compliance with these standards and continuous improvement, using this as a basis for synchronization of material flows and information, and to guarantee the quality and safety requirements, as well as the increase responsibilities and autonomy of people in the workplace. This is the main base of lean manufacturing (lean), which, associated with the concept of semi autonomous teams (this aimed at increasing the responsibility of the workers), significant operational success is bringing to companies that have deepened in its implementation and support. This thesis presents six case studies where there was the application of a model of standardized work and the concept of semi-autonomous teams simultaneously in different plants of the same company in the Brazilian chemical industry. The developed approach was the adaptation and application of a systematic model with five pre-defined steps, and structuring teams with semi autonomous work patterns as well as their roles and responsibilities. The model applied in the six cases studied was adapted based on a reference from the company's headquarters and research of market practices, adapted to reality and to the organizational structure of these units. The plants studied have different levels of technology and products portfolio, as well as operation time that distanced up to four decades of each other, where it was observed significant impact on speed of deployment, with different levels of acceptance by the leadership and comprehensiveness of results arising from the application of the model. Quantitative aspects of implementation, such as Safety, Quality and Productivity, which showed significant weight gains after the implementation of the model were evaluated and achieved medium gains of 25% in Productivity, 33% in Quality and 47% reduction in setup times as well as qualitative aspects such as absenteeism, the perception of those involved on the shop floor by the physical change in the working environment and the impact of standardization and systematization of activities on the motivation and performance of employees working in the semi autonomous teams. As conclusions, it´s discussed the potential constraints that influence the greater or lesser success of the applications in each of the six units.
Adubação com cama de galinha poedeira peletizada no desenvolvimento inicial da cultura do milho
(Universidade Federal de São Carlos, 2014-07)
Patroni, Bruno Henrique
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Soares, Marcio Roberto
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https://lattes.cnpq.br/6337462438517339
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https://lattes.cnpq.br/6337462438517339
The amount of commercial laying hens in Brazil is estimated at 90 million, with an average production of 2.20 kg of poultry litter per bird. Therefore, it is necessary to seek appropriate management practices for the final destination of the waste, in order to minimize environmental impacts. Transforming waste into a by-product with economic value has been an important strategy, especially as an alternative source of nutrients to reduce the production costs of important agricultural crops. The 2013/2014 Brazilian maize crop reached 78 million tons. For technified crops, with expected productivity above 10 t ha-1, the crop may require amounts of nitrogen (N) greater than 150 kg ha-1, of which a large part can be supplied by alternative organic sources, such as pelleted laying hen litter (PLHL). The objective of this study was to evaluate the use of PLHL, applied at different times, as a source of N for the initial development of maize, AG-8061PRÓ hybrid. The experimental design used was completely randomized, consisting of seven treatments with four replications: T1 - control; T2 - conventional chemical fertilization; T3 - organic fertilization with incorporation of PLHL at sowing; T4 - organic fertilization with PLHL split application at sowing and topdressing; T5 - organic fertilization with PLHL at sowing, followed by 50% of the dose indicated for topdressing chemical fertilization; T6 - organic fertilization with PLHL at sowing, followed by 100% of the dose indicated for topdressing chemical fertilization; T7 - organic fertilization with PLHL at sowing, followed by 200% of the dose indicated for topdressing chemical fertilization. The effect of treatments on the supply of N by the PLHL was compared with those obtained by the treatment that provided N with ammonium sulfate and by the control treatment without fertilization. The biometric parameters evaluated at 90 days after planting were: height, stem diameter, and dry biomass. The results were subjected to analysis of variance, with subsequent comparison of means by the Tukey test (p<0.05). The management of N from PLHL, whose content was 21.0 g kg-1, considered the annual mineralization rate of 10% throughout the experimental period, resulting in the application 80 t ha-1 of PLHL at sowing, in the treatment T3, and 14 t ha-1 in treatments T4, T5, T6, and T7. Miaze plants grown with PLHL, either exclusively or in combination with chemical fertilizer, showed better results in height, stem diameter, and dry biomass than plants grown with conventional chemical fertilization. The application of PLHL incorporated in the soil provided greater increments in the biometric parameters of the maize plants. Fertilization with PLHL, as the only source of N applied at sowing, promoted better results than chemical fertilization or chemical fertilization combined with organic. It should be noted that the organic source as PLHL provides N gradually, decreasing the chances of toxicity or leachate losses. In the case of complete replacement of the mineral source of N by PLHL, a large amount of material is necessary to supply the requirements of the maize crop, which residual effect benefits subsequent crops, such as winter crops. There is, however, the option of combining the application of chemical and organic sources of N, with satisfactory results when compared to conventional chemical fertilization.