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Uso de plantas medicinais para tratamento der síndromes respiratórias e suas aplicações por povos indígenas na pandemia da Covid-19
(Universidade Federal de São Carlos, 2021-01-21) Santos, Lívia Maria dos; Mattos, Sérgio Henrique Vannucchi Leme de; https://lattes.cnpq.br/8205497519881678; https://lattes.cnpq.br/6312298173944366
Ethnobotanical studies are important to contribute to conservation of biodiversity as well as the knowledge of traditional peoples, who interact constantly and deeply with other living beings, forming their own and unique relationships with nature. This work aimed to evaluate the use of medicinal plants to treat respiratory syndromes, especially by indigenous peoples. It also intented to make analysis fabout medicinal properties of the plants, the parts used and the forms of application as medicines The methodology was based on bibliographic research of data and information, both on the use of medicinal plants in the treatment of respiratory syndromes, especially by brazzilian indigenous peoples, as well as the impact of COVID-19 on these populations. In this study, the current pandemic moment was contextualized and highlighted how these people are combating the symptoms of COVID-19 with plants that have been known and used for some time. It was observed that there are groups of plants that are frequently used because they have important medicinal properties, as is the case of plants belonging to the Lamiaceae family. Both by the information conveyed by traditional media and, mainly, by alternative media, it is possible to verify that the Brazilian indigenous populations are using their traditional knowledge to treat symptoms associated with COVID-19 in order to mantain their resistance and resilience during the pandemic. Future research on this theme, aggregating and systematizing more data and information regarding the use of medicinal plants made by indigenous peoples during the pandemic, may collaborate in building a sustainable future based on a more ecocentric view of the relations between society and nature and subsidized by a scientific paradigm that favors a more systemic and open approach to dialogue between different human knowledge and practices.
Análise da expressão das proteínas PP2 em plantas com Huanglongbing e customização de um vetor de clonagem para silenciamento do gene PP2B10, por meio da tecnologia de RNA de interferência
(Universidade Federal de São Carlos, 2021-01-21) D’Alessandre, Nathália Da Roz; Granato, Laís Moreira; https://lattes.cnpq.br/9983674820658498; Schinor, Evandro Henrique; https://lattes.cnpq.br/2162760988837984; https://lattes.cnpq.br/4125672351303695
Huanglongbing (HLB) is one of the major phytosanitary problems in today's citrus culture. The commercial varieties of oranges, tangerines and tangelos are considered susceptible to HLB, while Poncirus trifoliata and some of its hybrids are considered tolerant. HLB is caused by the bacterium Candidatus Liberibacter asiaticus (CLas) and the symptoms have been related to callose deposition and the accumulation of phloem proteins (PP2) in the phloem-stained element tubes o of the infected plant, causing the obstruction of the tubes and inhibiting the transport of photoassimilated throughout the plant. In Arabidopsis thaliana, as PP2 are encoded by 30 genes, which are divided into two groups (AtPP2-A1 to AtPP2-A15 and AtPP2-B1 to AtPP2-B15). In this work, the first objective was to evaluate an expression of six genes that code phloem proteins (PP2) in Citrus sinensis (highly susceptible) and Poncirus trifoliata (tolerant), infected with CLas. For the RT-PCR experiments, ten plants of C. sinensis and P. trifoliata are inoculated with bubbles infected with CLas (HLB+) and five plants inoculated with healthy bubbles. A positive reduction in the genes obtained in HLB+ plants was observed in comparison with plants not inoculated for C. sinensis. For P. trifoliata, only the pp2B10, pp2B13 and pp2B15 genes were regulated positively. Positive expression of all genes obtained in C. sinensis was also observed when compared to P. trifoliata. Based on the results, the pp2B10 gene was selected to be used as a target for silencing by means of interference RNA (RNAi) technology. Thus, the second objective also exists in this work, which has been customized a cloning vector, using RNAi technology, for silencing the pp2B10 gene in sweet orange (C. sinensis). For this, fragments of the target gene were cloned in opposite escorts in the vector pHANNIBAL, and later, the hairpin construction under the control of the 35S promoter was subcloned into the vector pCAMBIA2301. The construction pCAMBIA2301 + hairpinpp2B10 is being used for genetic transformation of sweet orange to confirm the function of the gene and obtain plants tolerant to HLB.