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Saberes de diretores escolares: conhecimentos, experiências práticas e relacionais
(Universidade Federal de São Carlos, 2025-02-07)
Beltrami, Valcenir Aparecido
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Luiz, Maria Cecília
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https://lattes.cnpq.br/7425361719028650
;
https://orcid.org/0000-0001-5140-2753
;
https://lattes.cnpq.br/0380571875541361
The present research aims to analyze the balance of knowledge of principals who have passed public examinations, of the staff of a Municipal Department of Education (SME) of a medium-sized municipality in São Paulo, to verify how they knowledge should be constituted for the performance of their functions. The problem of the investigation was related to the issue of the filling of positions of Brazilian principals and to the understanding that there are figures of learning in theoretical, practical, and relational knowledge, according to Charlot's Relationship with knowledge (2000). This qualitative research, with an exploratory approach, conducted an empirical part with a methodology divided into two stages: the first, called Balance of Knowledge (Charlot, 2000) and, the second, Focal Group. Ten directors were invited to participate, each director represented one of the ten administrative regions of the SME. The data were analyzed and divided into three constellations/dimensions, according to the theory of Relationship with Knowledge: 1) Knowledge of the profession: validation and understanding of the functions of the principal; 2) Technical-rational knowledge: needs of the organization and of the school daily life; 3) Knowledge of coexistence: conflict situations, dialogue and active listening. It is concluded that the school principals who participated in the research constituted and constitute theoretical, practical, and relational knowledge when they validate their functions, mainly related to technical-rational doing. When they face difficulties in relational dealings, conflicts, or school violence, they use theoretical foundations that they would acquire in their training, but always valuing practical and relational activities, which are the drivers of their decisions.
Rotulagem nutricional frontal na América do Sul: uma análise comparativa das regulamentações
(Universidade Federal de São Carlos, 2025-02-07)
Barbosa, Rayssa Azuza Ichimura Gonçalves
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Matos, Andreia Pereira
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https://lattes.cnpq.br/6155202940087696
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https://orcid.org/0000-0001-9502-116X
Nutritionally unbalanced products have contributed to the alarming increase in chronic non-communicable diseases, such as obesity, diabetes and cardiovascular problems. In this context, front-of-pack nutrition labeling has emerged as an important regulatory strategy to inform consumers about the risks associated with excessive consumption of certain ingredients, promoting more conscious food choices. Countries such as Chile, Argentina and Brazil have adopted different approaches to implementing these labels, with emphasis on the use of clear and easy-to-read nutrition labels. The successful implementation of these regulations has the potential to generate a positive impact not only on consumer health, but also on innovation and transparency in the food industry. Therefore, front-of-pack nutrition labeling in South America is a reflection of the social and economic transformations in the region, with direct implications for public health and the business models of food companies. A comparative analysis of the different models adopted by South American countries offers a broad view of the challenges and opportunities present in this constantly evolving scenario.
“Big in Japan”: Análise das transformações, interações e encadeamentos estruturais do Japão e Estados Unidos entre 1965 e 2000
(Universidade Federal de São Carlos, 2025-02-07)
Leite, Lucas Martins
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Oliveira, Maria Aparecida Silva
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https://lattes.cnpq.br/3455553543736709
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https://orcid.org/0000-0001-7729-4068
This paper proposes a comparative analysis of the economic structures of Japan and the U.S. between 1965 and 2000. Through the lens of input-output analysis, the internal policies of both countries, and their bilateral trade, the influence that industries and sectors—grouped by technology—exerted on each other across the Pacific is assessed within the context of rivalry between a dominant U.S. and a rising Japan. Using regional input-output matrices, the study explores the evolution of sectoral linkages at both domestic and interregional levels, as well as the sectors that drove each country's growth. The analysis verifies the interdependence between differentiated and scale-intensive sectors in both countries, evidenced by their high common interregional linkage indices. Structural differences shaped interregional spillovers and trade patterns in both countries. While Japan expanded its exports in differentiated and scale-intensive industries, the U.S. maintained a less prominent presence in these sectors, with a greater share of resource-intensive industries, despite measures taken to reduce these asymmetries.
Análise de concentração e rivalidade no mercado de aviação civil no Brasil: um cenário hipotético de duopólio
(Universidade Federal de São Carlos, 2025-02-07)
Pereira, Renan Pegas
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Pitelli, Mariusa Momenti
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https://lattes.cnpq.br/2810339285129049
The present study aims to analyze market concentration and indications of rivalry in the Brazilian civil aviation passenger transport sector, considering a hypothetical merger scenario between Gol and Azul in the main domestic air routes. To achieve this, market share data, the Herfindahl Hirschman Index (HHI), and the relative market share instability test were applied to the most demanded routes in Brazil between January 2021 and July 2024. Among them, the key routes analyzed include Rio de Janeiro – São Paulo (SBRJ – SBSP; Santos Dumont and Congonhas airports), São Paulo – Brasília (SBSP – SBBR; Congonhas and Presidente Juscelino Kubitschek airports), and Recife – Guarulhos (SBRF – SBGR; Recife/Guararapes International and Guarulhos airports). In the current scenario, high levels of concentration are observed. In the hypothetical merger scenario, concentration increases significantly, establishing a dominant position for the new company and practically reducing the market to a duopoly. The ADF tests indicate instability in market share participation in the hypothetical scenario, which may suggest rivalry. The results emphasize the need for a thorough and careful competitive assessment in the event of a merger or acquisition in the sector.
Etnografia da luta infinita: assentamento e expulsão no Acampamento Capão das Antas (São Carlos, SP)
(Universidade Federal de São Carlos, 2025-02-07)
Savarego, Júlia Aricó
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Villela, Jorge Luiz Mattar
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https://lattes.cnpq.br/8301325600502054
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https://lattes.cnpq.br/1852273495891237
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https://orcid.org/0000-0002-6524-4415
This dissertation aims to ethnograph the resistance strategies of the residents of Acampamento Capão das Antas, a rural occupation fighting for Agrarian Reform located in the municipality of São Carlos, in the east-central region of the state of São Paulo, Brazil, in response to a currently suspended land repossession process. This process, which began in November 2011 and is now moving toward the establishment of a settlement, was legally grounded in environmental legislation. According to the group of residents with whom this work is conducted, the research concerns the creation of a documentary to write the history of the camp’s struggle. The methodological projections resulting in this text weave together an ethical-philosophical commitment, but also an aesthetic one, guiding the research’s execution. Thus, it makes sense to think of methodology necessarily as ethics expressed in the aesthetics of the text, which presents itself as a documentary—a ‘storytelling of the struggle.’ The documentary is simultaneously method, object, and research goal. I aspire for the text to be entangled as part of the struggle and to have its own political agency, given the understanding and commitment to the idea of writing as a world-creating practice. But, as the title of this dissertation announces, what does it mean to finish writing an endless struggle? In a context where exhaustion and depression were the most recurring themes, I raised the following problem: How can the certainty of the unattainable be precisely what keeps people fighting? The making of ‘Anthropology in and with the Endless Struggle’ initially led me to perceive a tension—and often contradictions—that, throughout the fieldwork begun in 2018, proved to be my own. From this realization, I wove oppositions into the ethnographic writing in an attempt to echo the movement of the struggle itself, such as the interdependent relationship between eviction and settlement in the current design of public policies aimed at land redistribution. This dissertation deals with the entanglements of hope and failure that mark what is a collective project: making home through land as a connector of temporalities, people, plants, and non-humans. I argue that the land struggle of the people of Acampamento Capão das Antas is not only about obtaining legal use but also about the constant work of forging bonds with the land, memory, and one another. And these bonds can only be sustained through care—for and with the land and the people, living and dead. It is because there are ‘affective bonds’ that people choose to stay or leave, and the affect involved is not just love and hope but also its relationship with pain and failure. This movement is echoed in this ethnography as a trigger for building comparative and imaginative bridges, enhancing life and projecting other possible futures.
Análise não linear geométrica e física global/local de pilares de edifícios em concreto armado
(Universidade Federal de São Carlos, 2025-02-07)
Corelhano, Angelo Giovanni Bonfim
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Christoforo, André Luis
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https://lattes.cnpq.br/7623383075429186
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Almeida Filho, Fernando Menezes de
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https://lattes.cnpq.br/0447825383222965
In this thesis, aspects inherent to the nonlinear physical and geometric analysis of columns of bracing structures for buildings in reinforced concrete will be addressed. The paper focused on the structural analysis of the columns through the use of a computational tool capable of performing rigorous non-linear analysis, studying conventional models using the finite element method. The inertia reductions in the columns are evaluated, as well as the effects of nonlinearities in global and local analysis in an integrated way in a single model. The models were previous designed to present reinforcement rates lower than the maximum allowed by ABNT NBR 6118:2014. Models with three different relationships between columns sides and three concrete strength classes were analyzed. The results showed that the stiffness of beams and columns for global modeling is close to those proposed by ABNT NBR 6118:2014 for concrete C25 and indicates that they are lower for higher concrete classes, in addition to indicating that the relative stiffness beam/column is lower than 0.5 as given by the aforementioned standard. Local second-order effects were very small, even for the most displaceable models.
Migração e redes digitais: narrativas e apoio entre brasileiros em Londres
(Universidade Federal de São Carlos, 2025-02-10)
Torres, Letícia de Lucca
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Machado, Igor José de Renó
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https://lattes.cnpq.br/4705450652848752
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https://orcid.org/0000-0001-7811-2641
This study analyzes how digital social networks, focusing on YouTube, influence the migration experience of Brazilians in London, serving as a pedagogical space for migrants and potential aspirants. Using a netnographic approach and qualitative content analysis, channels such as Nathalia Lambranzi, Livrestrada, Guri in London, Vida na Inglaterra, Na Vibe, Realidade Inglesa-Londres, Alvaro Azevedo, Enjoy Londres, Everton Eduardo-Vida de Imigrante UK, Situação do Imigrante, Nova Utopia, Destino Inglaterra, DK Vlogs, and Uai Londres were studied, as well as interactions that shape narratives (video comments), offering emotional support, practical information, experience sharing, cultural insights, and the creation of support networks. The research reveals how these platforms facilitate migrants' transition and adaptation, promoting the construction of hybrid identities, professional opportunities, social connections, and spaces of belonging. Furthermore, it discusses the contradictions within migration narratives, which fluctuate between romanticization and lived reality, influencing expectations, daily challenges, and future perspectives.
Quantificação de elementos potencialmente tóxicos em sombras para olhos
(Universidade Federal de São Carlos, 2025-02-10)
Zeppini, Anna Claudia Tocchio
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Carvalho, Mayara de Almeida Ribeiro
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https://lattes.cnpq.br/6211067303954955
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https://orcid.org/0000-0001-8143-318X
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Oliveira, Luciana Camargo de
The presence of potentially toxic elements in cosmetics, especially in eye shadows, is an increasing concern due to the health risks associated with these elements. Metals such as cadmium (Cd), chromium (Cr), nickel (Ni), and lead (Pb), which are often used in metallic pigments, can exceed safety limits, causing irritation, dermatitis, and, in cases of prolonged exposure, systemic toxicity. Strict regulation of raw materials and manufacturing processes is crucial to ensure consumer safety. Studies on the composition of cosmetics are essential to improve regulations and mitigate health risks. In this context, this study aimed to quantify Cd, Cr, Ni, and Pb in samples of eye shadows from different brands and shades, as well as to discuss the potential health effects on users. The samples were subjected to microwave-assisted digestion and then quantified by Microwave Plasma-Atomic Emission Spectrometry (MP-AES). The results indicated that while Cd, Ni, and Pb were below the detection limits, Cr was found in concentrations above the limit set by ANVISA (1 ppm) in all samples, particularly in more intense shades like brown and wine. The findings may be associated with quality control failures in manufacturing and high light concerns about contamination, considering the health risks. Therefore, it is recommended to implement more effective quality control measures and strengthen regulation.
A conlang como método de ensino de conceitos linguísticos: problemática e possíveis encaminhamentos
(Universidade Federal de São Carlos, 2025-02-10)
Girardi, Jane Eder
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https://lattes.cnpq.br/3939612232315995
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https://orcid.org/0000-0002-3542-9945
Terapia Ocupacional e Saúde Mental na e com a comunidade: análise de uma experiência
(Universidade Federal de São Carlos, 2025-02-10)
Roman, Loise Carlos
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Minatel, Martha Morais
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https://lattes.cnpq.br/3621113172277748
This study aims to analyze my experience as an Occupational Therapy student in a mental health promotion group within the community, coordinated by an Occupational Therapist. Based on the principles of Popular Education and Psychosocial Care, the project “Território do Cuidar”, immersed in the social dynamics of oppressions, suffering, and possibilities, seeks to enable the production of life, meaning, and sociability through collective construction and the valorization of popular knowledge. The objective was to understand how the experience as an extensionist contributed to my education, using the experience report to describe and reflect on the practices experienced. It was observed that the approach to the community and territory revealed the potential of the collective, in which, through welcoming, belonging, sharing, lovingness and affection, it is possible to generate transformation and promote mutual and shared care that overflows into the daily lives of the participants. It is concluded that the experience in “Território do Cuidar” allowed me to be affected by the practices experienced, generating transformations, guiding my education and professional practice, and expanding my understanding through the diverse practices of Occupational Therapy. This experience reaffirms the importance of the university’s approach and joint construction with the territory, in order to promote a practice that is increasingly contextualized and transformative.
Educação Popular em Saúde e infâncias: Uma análise das produções nacionais sobre práticas educativas e de cuidado
(Universidade Federal de São Carlos, 2025-02-10)
Poiatti, Gabriela Gonçalves
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Minatel, Martha Morais
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https://lattes.cnpq.br/3621113172277748
This study analyzes national productions on Popular Education in Health (EPS) in the context of childhood, highlighting the potentials and challenges of this practice in Brazil. Based on the principles of Paulo Freire, EPS aims to promote a humanized, dialogical, and participatory approach to healthcare. The objective was to identify and analyze scientific articles on the application of EPS with children, seeking care practices and the development of inclusive public policies. The methodology adopted was a narrative literature review, selecting open-access articles published in the last 20 years, through searches in Scielo, BVS, and the CAPES Periodicals Portal. The research identified a lack of studies focused on children, particularly at the intersection with EPS. The results indicate that EPS practices for childhood are still underexplored, with more emphasis on adult and social vulnerability contexts. Furthermore, the studies highlight the need for robust institutional and political support for the implementation of these practices. The final considerations emphasize the importance of strengthening EPS public policies, aiming for the integration of various sectors and the creation of care spaces that respect children's uniqueness and rights, with a focus on expanding research on the topic.
Uso de macrófitas aquáticas como espécies fitorremediadoras no tratamento terciário de efluentes de laticínios
(Universidade Federal de São Carlos, 2025-02-10)
Camargo, João Vitor
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Schwantes, Daniel
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https://lattes.cnpq.br/5254472099796913
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https://orcid.org/0000-0002-2251-5887
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Baron, Daniel
The possible environmental contamination by heavy metals (HMs), such as cadmium (Cd), lead (Pb), and chromium (Cr), has become recurrent due to the increase in anthropogenic activities when these do not meet the correct technical recommendations. Thus, in order to minimize the damage caused by irregular activities, phytoremediation emerges as an efficient and cost-effective technique in which plants are used for environmental decontamination. In this context, the aquatic macrophytes Pontederia crassipes (Mart.) Solms (arrowhead water hyacinth), Pistia stratiotes L. (water lettuce), and Salvinia auriculata Aubl. (giant salvinia) stand out, as they have remediation potential for inorganic organic pollutants present in water bodies. The dairy industry is present throughout the national territory, and when this industrial sector does not properly manage its waste before discharging it into water bodies, severe cases of contamination occur. Therefore, we tested the hypothesis that aquatic macrophytes have phytoremediation potential as a biotechnological tool in the tertiary treatment of dairy effluents contaminated with pollutants. Our objective was to evaluate the phytoremediation efficiency of macrophytes in water bodies contaminated by heavy metals. We adopted a completely randomized experimental design, consisting of the species P. crassipes, P. stratiotes, and S. auriculata cultivated in dairy effluents containing heavy metals (Cd and Pb). To achieve this, we conducted analyses of physicochemical parameters, total dry matter mass (DMM) to indicate good plant adaptation, and analyzed the concentrations of toxic elements in plant tissues to understand HM accumulation. The collected data were subjected to Tukey and Friedman tests, and after data processing, we created time series graphs for the analyzed variables. The literature reports the existence of a family of protein transporters called 'ZIP', which is involved in the transport of Fe, Zn, Mn, and Cd. Other proteins and transporters stand out and show affinities with divalent ions. We speculate that the transport of potentially toxic elements (PTE) occurs through high- to low-affinity systems (HATS and LATS, respectively). The PTE concentrations were not detrimental to the vegetative growth of the macrophytes, and we highlight their ability to "polish" the physicochemical parameters of the effluent, demonstrating the efficiency of these plants in adjusting the effluent quality before its discharge into water bodies. Therefore, we accept our initial hypothesis that aquatic macrophytes have phytoremediation potential as a biotechnological tool in water bodies contaminated with pollutants.
A importância do brincar na alfabetização e no letramento na educação infantil
(Universidade Federal de São Carlos, 2025-02-10)
Pereira, Emily Larissa Bortolott
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Monteiro, Maria Iolanda
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https://lattes.cnpq.br/4189205834370563
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https://orcid.org/0000-0002-4534-1437
The paper entitled The Importance of Play in Literacy Development in Early Childhood Education investigates how playful practices are presented for the development of initial reading and writing skills in Early Childhood Education children. A bibliographic and qualitative research study analyzes the relationship between playing and learning. For this purpose, a bibliographic survey was carried out, from October to November 2024, in the Brazilian Digital Library of Theses and Dissertations (BDTD). From this survey, postgraduate works related to the proposed theme were selected and, still using official documents such as the National Common Curricular Base (BNCC), the National Curricular Guidelines for Early Childhood Education (DCNEI) and the Statute of the Child and Adolescent (ECA). The results indicate that playing is a central pedagogical tool, promoting not only cognitive and motor development, but also social, emotional and linguistic development. Singing games, storytelling, pretend play and activities involving sounds and rhymes are strategies that stimulate phonological awareness, creativity and interest in the world of literacy. In addition, the role of play in the development of children's autonomy, critical thinking and ability to express themselves is highlighted. However, the study also highlights challenges, such as the lack of teacher training to plan intentional playful activities and the pressure for early academic results, which can hinder the effective use of play in literacy. The research reinforces the need for public policies that value playfulness as a pedagogical strategy and guarantee the ongoing training of educators. It is concluded that playfulness is essential for meaningful and inclusive literacy, respecting the rhythm and particularities of each child. Playfulness should be understood as a right and a necessary practice in the daily routine of Early Childhood Education, promoting comprehensive development and preparing children for the challenges of Elementary School and life in society.
Detecção heteródina do campo elétrico da luz transmitida por amostras atômicas
(Universidade Federal de São Carlos, 2025-02-10)
Pereira, Thales Martins Batista
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Teixeira, Raul Celistrino
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https://lattes.cnpq.br/6108339817883159
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https://orcid.org/0000-0001-8358-861X
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https://lattes.cnpq.br/2957525493396643
This work presents the heterodyne detection technique for light electric fields, a photodetection method capable of measuring both the amplitude and phase of an electromagnetic field. Applied to the context of light-matter interaction, the study of this photodetection technique allows the complete characterization of the electric field of light coherently transmitted by a cloud of cold atoms. Thus, after presenting the Beer-Lambert Law, which governs the behavior of light intensity coherently transmitted by atomic samples, and the principles behind the heterodyne detection technique, the construction of the experimental setup is detailed, explaining the particularities of the optical and electronic circuits, as well the digital demodulation carried out in Python language in order to treat the experimental data. Finally, as the main results of this work, a system calibration stage is carried out, capable of detecting light powers in the order of picoWatts. Furthermore, with digital demodulation, phase acquisition occurs with satisfactory precision. Finally, with the aim of investigating transmission through atomic samples, the system was used to capture the amplitude of the signal transmitted by a liquid sample.
Desenvolvimento de software aplicativo para diagnóstico de doenças, pragas e distúrbios fisiológicos em tomateiro (Solanum lycopersicum L.)
(Universidade Federal de São Carlos, 2025-02-10)
Almeida, Felipe Henrique Millk de
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Oliveira, Anna Hoffmann
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https://lattes.cnpq.br/6666918682171234
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https://orcid.org/0000-0002-5479-8359
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https://lattes.cnpq.br/6359216243662195
Tomato (Solanum lycopersicum L.) holds significant economic importance in Brazil; however, a wide range of diseases, pests, and physiological disorders can affect its production. Therefore, this study aims to develop a software application for the diagnosis of diseases, pests, and physiological disorders in tomato crops, based on symptoms and signs that are easily identifiable by producers. A database was created in MySQL, containing signs, symptoms, and management recommendations. The interface and diagnostic logic were developed in Java, allowing the producer to select the observed symptoms. The application operates interactively, enabling the producer to gradually select the symptoms observed in their plants. Based on this information, the system analyzes and provides an accurate diagnosis, along with recommendations for appropriate management. The efficiency and functionality of the application were evaluated through usability and integrity tests. Since Test 1, the software showed satisfactory usability performance, with 100% approval regarding its intuitive interface and response speed. The main challenge was related to agronomic information. In Test 2, diagnostic accuracy increased from 5% to 95%, and agreement with management suggestions rose from 5% to 80%, with a significant decrease in errors. The application received 100% recommendation. Despite not achieving 100% accuracy, the errors were occasional, with some problems being difficult to diagnose due to symptoms similar to other issues. The evolution of the application increased its accuracy, speed, and usability, making it more reliable and recommended. Thus, it can be concluded that the application has great potential to assist in the identification and management of phytosanitary problems, with possibilities for expansion to other crops and integration with machine learning to further enhance diagnostic accuracy.
Diferentes formas de obtenção do geraniol
(Universidade Federal de São Carlos, 2025-02-10)
Mandarino, Giovanni Catossi
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Schwab, Ricardo Samuel
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https://lattes.cnpq.br/6935890756504964
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https://orcid.org/0000-0001-8722-9403
Essential oils are a mixture of volatile and lipophilic molecules found in plants. These substances are widely used in the cosmetic and pharmaceutical industries due to their specific properties. Thanks to their characteristic aromas, they play a historical and essential role in the fragrance and perfume market. This relevance is confirmed by the constant growth in revenue within Brazil's personal hygiene and cleaning sector. One example is geraniol, an acyclic monoterpenoid alcohol found in rose leaves, which has a light floral rose note. Among the various existing extraction methods, only steam distillation allows for satisfactory yields of geraniol. However, due to the increasing demand for essential oils, extraction exclusively from natural sources has become insufficient to meet global market needs. This, in turn, has contributed to the development of more efficient synthetic methodologies for obtaining these molecules. Thus, geraniol can be synthesized in four reaction steps from β-pinene or obtained from citral through the reduction of the aldehyde group. Additionally, geraniol can be produced through biosynthetic pathways based on the mevalonate pathway, replicating the natural process that occurs in plants.
O turismo diaspórico como prática de aquilombamento
(Universidade Federal de São Carlos, 2025-02-10)
Gonçalves, Elisa de Paula
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Ferragi, Cesar Alves
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https://lattes.cnpq.br/8059661338196579
;
https://orcid.org/0000-0001-8066-500X
;
http://lattes.cnpq.br/2569654740506166
This study explores diasporic tourism as a practice of quilombo formation, analyzing its impact on the reaffirmation of the identity of Black people. Using a qualitative approach, with interviews conducted with Black Brazilians, we investigated how these experiences promote cultural and emotional ties with ancestry, strengthen self-acceptance, and contribute to resistance against structural racism. Diasporic tourism, as a practice of connecting with the roots and communities of the diaspora, reveals itself as a transformative strategy for mental health and the appreciation of Afro-descendant culture. The research seeks to broaden the understanding of the role of tourism in the emancipation and belonging of Black communities.
Impactos ambientais e soluções sustentáveis para o descarte de máscaras faciais descartáveis: reciclagem e redesign
(Universidade Federal de São Carlos, 2025-02-10)
Nardini, Larissa Batista
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Cruz, Sandra Andrea
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https://lattes.cnpq.br/9632409046763256
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https://lattes.cnpq.br/0763898878050614
The COVID-19 pandemic has increased the production and use of disposable face masks made of non-woven fabric. Although essential for health control, their improper disposal has caused significant environmental impacts, such as the increase in microplastics in terrestrial and aquatic ecosystems. This study reviews all the environmental impacts caused by the improper disposal of masks and, consequently, the challenges for recycling. This study provides a review of the complete composition of the mask, indicating that it is a material composed of different polymers, namely polypropylene, polyethylene terephthalate, polyethylene and polyurethane, and the presence of a metal found in the moldable region of the nose. Therefore, with the complex and variable composition of the mask, attempts to recycle it result in an immiscible and incompatible blend, which makes the process even more difficult. Therefore, the solution to this problem may be the redesign and production with a single component, polypropylene. This study concludes that, to mitigate environmental impacts, it is essential to integrate more efficient recycling strategies and processes, thus reducing pollution caused by the improper disposal of disposable masks.
Otimização de protocolos de expressão e purificação de metiltransferases de ZIKV e YFV
(Universidade Federal de São Carlos, 2025-02-10)
Dantas, Letícia Piazentin
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Fuentes, Andrea Soares da Costa
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https://lattes.cnpq.br/2426157257540389
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https://orcid.org/0000-0002-7379-1136
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Mesquita, Nathalya Cristina de Moraes Roso
This study aimed to optimize protocols for the expression and purification of methyltransferases from Zika (ZIKV) and Yellow Fever (YFV) viruses, which belong to the Flavivirus genus, which includes important pathogens associated with epidemic outbreaks and serious complications, such as microcephaly, Guillain-Barré syndrome and hemorrhagic hepatitis. These viruses have RNA genomes with a 5' cap modified by methyltransferases (MTase), ensuring greater stability and evasion of the host's immune system. To explore these proteins as potential therapeutic targets, expression tests were performed in Escherichia coli Rosetta DE3, identifying the ideal conditions as plasmid M11, ZYM 5052 medium and a temperature of 18°C for methyltransferase from the virus that causes Yellow Fever and for methyltransferase from the virus that causes Zika Fever. YFV methyltransferase purification was performed by affinity and molecular exclusion chromatography, resulting in proteins of high purity and stability in HEPES (NaOH) buffer (pH 7.5, 500 mM NaCl and 5% glycerol), which is ideal for functional preservation of the protein. However, Zika virus methyltransferase showed lower efficiency in the purification process, which negatively impacted its stability and final yield.This study provides a solid basis for expression and purification of methyltransferases for futurebiochemical and structural investigations, in addition to contributing to the advancement in thedevelopment of antiviral strategies against these flaviviruses. The next work steps include the optimization of the ZIKV methyltransferase purification protocol, biophysical, biochemical and structural characterization of the methyltransferases, using techniques such as size exclusion chromatography (SEC) with multiangle light scattering (MALS) (SEC-MALS), differential scanning fluorimetry (DSF), circular dichroism (CD), fluorescence spectroscopy and X-ray crystallography, in addition to functional analysis of enzymatic activity.
Desenvolvimento e caracterização físico-química de molho beterraba, goiaba e tomate
(Universidade Federal de São Carlos, 2025-02-10)
Branco, Nathalia Beatriz
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Silva, Thaís Jordânia
;
https://lattes.cnpq.br/4582114700719319
The growing demand for convenience foods and the need for sustainable solutions for the use of fruits and vegetables have boosted the sauce market by enabling the use of perishable raw materials and adding value to the product. This study aimed to develop and characterize sauces based on guava and beetroot, exploring the potential of these ingredients, known for their high nutritional value and for contributing to sustainability by minimizing the waste of fresh food. The study consisted of developing sauces with different formulations, using varied combinations of tomato, guava and beetroot pulp. Physicochemical analyses were performed, including pH, soluble solids content, titratable acidity, water activity, color and viscosity. The guava sauce stood out for its high acidity, while the beetroot sauce presented intense color and high viscosity. In addition to the technical aspects, the study highlighted the positive impact on reducing waste and valuing local ingredients, promoting a sustainable approach to the development of new products. The use of agricultural surpluses and the transformation of perishable fruits and vegetables into sauces with a longer shelf life contribute to mitigating losses throughout the production chain, aligning with food safety and market innovation guidelines. It is concluded that the sauces developed not only meet the practicality and health expectations of modern consumers, but also represent a significant opportunity for the expansion of the sustainable food market in Brazil.