Mecanismo de ação da cedrelona na morte celular programada de células de câncer de mama
Becceneri, Amanda Blanque
MetadataShow full item record
Cancer is among the leading causes of death in the world and breast cancer is the type of cancer that affects more women. The treatment of this disorder requires a careful selection of one or more methods of intervention. The majority of drugs currently used in chemotherapy causes DNA damage in tumor cells and also in normal cells, therefore the development of new chemotherapeutic agents is challenging, since these chemotherapeutic agents should be able to kill or inhibit the growth only of tumor cells. From ancient times until today plants and their products have been used for the treatment of diseases, thus the natural compounds may provide new opportunities for the development of cancer drugs. Cedrelone is an isolated limonoid from methanol extract of Trichilia catigua aryl, a native Brazilian plant that grows abundantly in various regions of the country and is popularly known as "cedrinho", "catuaba" or "catiguá". Studies have shown that cedrelone has insecticidal and antifungal activity and previous studies performed by our research group have demonstrated that cedrelone is able to inhibit proliferation, colony formation, cell adhesion, migration, invasion and moreover, to induce apoptosis in triple negative breast cancer cells from the line MDAMB- 231. Therefore, the aim of this study was to identify the mechanism of action of cedrelone in triple negative breast tumor cells, MDA-MB-231, through cedrelone analysis, and through cell morphology assay, colony formation, apoptosis assays such as DNA fragmentation and nuclear fragmentation, cell cycle, qRT-PCR and western blotting. The results demonstrate that cedrelone has an affinity for biological membranes and no antioxidant activity. Furthermore, cedrelone is capable of inducing morphological changes, reduce the number and size of colonies and induce apoptosis by increasing the gene expression of pro-apoptotic protein Bax and decreasing gene expression of anti-apoptotic Bcl-2 in MDA-MB-231. These results indicate that cedrelone has immense potential in cancer therapy based on their antiproliferative and apoptosis inducing effects.
Showing items related by title, author, creator and subject.
Desenvolvimento de dispositivos descartáveis e de baixo custo para a detecção de biomarcadores relacionados ao câncer de colo uterino e endometrial Baldo, Thaisa Aparecida (Universidade Federal de São Carlos, UFSCar, Programa de Pós-Graduação em Química - PPGQ, Câmpus São Carlos, 31/05/2019)Cervical cancer (CC) and endometrial cancer (EC) are malignant neoplasms that affect women worldwide and the early diagnosis of these cancers is still limited. The incidence of these malignancies has been increasing year ...
Efeito de um complexo de rutênio em células de câncer de mama em diferentes modelos 2D e 3D in vitro e in vivo Becceneri, Amanda Blanque (Universidade Federal de São Carlos, UFSCar, Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF, Câmpus São Carlos, 18/10/2019)Cancer is among the leading causes of morbidity and mortality in the world. Specifically, breast cancer is an extremely heterogeneous disease, being composed of different subtypes and this heterogeneity makes treatment ...
Teles, Ramon Handerson Gomes (Universidade Federal de São Carlos, UFSCar, Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF, Câmpus São Carlos, 06/09/2019)Breast cancer is the second most common type in the world and can be considered a disease with good prognosis if diagnosed early and treated timely. The treatment of breast cancer aims to increase survival and improve the ...