Estudo dos efeitos do laser de baixa intensidade na angiogênese em ruptura parcial do tendão calcâneo de ratos.
Abstract
The purpose of this study was to analyze the 660 nm GaAlAs laser effect with 10 mW and
40 mW, during 10 seconds, on angiogenesis process and repair quality after partial lesion of
Achilles tendon in rats. It was used 104 male rats, that 96 rats had been submitted to lesion
by a falling of 186 g from a height of 20 cm, above the right tendon. These rats had been
randomly divided in 3 groups, treated in 3 (n = 32), 5 (n = 32) and 7 (n = 32) days,
respectively. Each of these groups was divided again into 4 subgroups, which were
submitted to laser with mean output of 10 mW (n = 8) and 40 mW (n = 8), a sham
treatment (n = 8), and a group with no treatment (n = 8). The analyzes were made by
vessels count that have been previous prepared with nanquim ink and by a semiqualitative
analyze of polynuclear cells presence, mononuclear cells presence, collagen organization
and neovascularization. A fourth group with 8 animals was established as control, without
lesion and used as reference for qualitative analyze. The comparison of the groups made
evident that the animals submitted to laser showed more vessels number than the control
groups (p<0,05) and the 40 mW subgroup showed better vascularization than the others
after 3 days of irradiation. (p<0,01). The semiqualitative analyze showed statistical
difference (p<0,05) when considering collagen organization and neovascularization in the
comparison between the 10 mW, 40 mW and control. Qualitatively, the groups treated with
laser showed better repair aspect. The results permit us to conclude that Low Level Laser
Therapy with 660 nm promoted an increase of the blood vessels number and better repair
quality and the mean output of 40 mW had begun the neovascularization precocious,
showing significant difference after 3 days of application.