Aplicação de técnicas de análise de imagens na quantificação da concentração celular no cultivo de células de inseto

Abstract

Paradoxally, in spite of the cellular concentration to be the most important variable in a bioprocess, in practice is the less monitored as a consequence of the experimental difficulty found in its quantification in an aseptic, non invasive, discriminative (viable and not viable), in real time and, above all, in reliable way. For this reason, little progress has been reached in the quantification of cellular concentration in bioprocesses, situation that creates difficulties for the application of knowledge based techniques for control and bioprocesses optimization in large scale. The analysis of digital images is being investigated as a promising tool for quantification of the cellular concentration seeking for monitoring and control of bioprocesses. In this work, the growth of the insect cells Spodoptera frugiperda (Sf9) and Dosophila melanogaster(S2), was accompanied when cultivated in Schott shaken flasks, in complex medium in temperatures and pHs convenient for each species. The new Wavelet transform was used for the processing of the images making use of an application of MATLAB 5.2 and the obtained results along the cultures with the two cells were compared with experimental measurements of cellular concentration and with computational measurements of Densitometry of the acquired images. The results indicate that the Wavelet transform technique as well as the Densitometry are potential alternative for estimation the cellular growth of insect cells.