Estudo químico do fungo endofítico isolado de Hortia superba (Rutaceae) na produção de compostos secundários
Abstract
The order
Sapindales, compost for the families Rutaceae, Meliaceae, Burseraceae,
Simaroubaceae, Bierbersteiniaceae, Kirkiaceae, Anacardiaceae, Sapindaceae and
Nitrariaceae, is rich in various classes of speciais metabolites, being the most
frequents: coumarins, alkaloids, flavonoids, quassinoides and limonoides. The family
Rutaceae is characterized by a great diversity of those metabolites not common in other
families the order (Silva et al., 1988) and is constituted by different genera, of which
stands out Hortia, compost for five species distributed from Panama to the state of São
Paulo, and some of the area Amazônica (Groppo and Pirani 2012), besides of to be
endemic in the area of Minas Gerais, where take the plant as a sample. In this work
the endophytic fungus belonging to the family Xylaria, was isolated .This fungus was
inoculated in the malt extract agar culture medium, where was produced several
secondary metabolites, and between they were separated and identified three
compounds: Declorogriseofulvin, Griseofulvina, 3,4-Dihydro-8- hydroxy-3-
methylisocouarin-5-carboxylic acid, Spiro[benzofuran-2(3H),1'-[2] cyclohexene]-3,4'-
dione, 5-cloro-7-declorogriseofulvina, a compound that is still unpublished and 5-
clorogriseofulvin. The second compound is isomer of this last substance and was
identified by the NMR and Massa Spectrometry techniques. The hemolytic activity in
blood was measured for the substances, resulting the griseofulvin being less toxic in
blood than your analogue, as declorogriseofulvin. In addition, the biological activity of
the compounds was analyzed against to the strain Staphylococcus aureus, by the
Inhibitory Concentration Method for bacteria (MICs) (Barbosa. S et al, 2016),
concluding as result that the substances do not have antimicrobial activity against the
Gram- positive bacteria.